MyavA.00304.a

Cysteine desulfurase

CENTER ID: MyavA.00304.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00304.a.A1.PS00583 Full length( MyavA.00304.a ) 1 408
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_128800
PATRIC ID: fig|243243.7.peg.3243
RefSeq: YP_882497.1
UniProt: A0A0H2ZWX6
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAIRADFPI LKRIMRSGNQ LAYLDSGATS QRPLQVLDAE REFLLTSNGA VHRGAHQLME ESTDAYEQGR ADIAAFVGAD PDELVFTKNA TEALNLVSYV VGDNRFERAV GPGDVIVTTE LEHHANLVPW QELARRTGAS LRWYGVTDDG RIDLDSLQLD ERVKVVAFSH HSNVTGAVAP VAELVGRARA VGALTVLDAC QSVPHQPVDF HALDVDFAAF SGHKMLGPNG IGVLYGRREV LSQLPPFLTG GSMIETVTME SSSYAPVPQR FEAGTPMASQ VVGLAAAARY LGALGMDAVQ AHEERLVAAA IEGLSGIDAV RIVGPTSTEN RGSPVSFVVD GVHAHDVGQV LDDEGVAVRV GHHCALPLHR RFGLAATARA SFAVYNTAEE VDRLVAGVRR ALDFFGGA
NT Sequence
GTGGCCGCGA TCCGCGCCGA TTTCCCCATT CTGAAACGGA TCATGCGCAG CGGAAACCAG TTGGCGTACC TGGATTCCGG TGCGACGTCG CAACGCCCAC TGCAGGTGCT CGACGCCGAG CGGGAATTCC TGCTGACCTC CAACGGCGCC GTGCACCGCG GTGCGCACCA GCTGATGGAG GAGTCCACCG ACGCCTACGA GCAGGGCCGC GCCGACATCG CGGCGTTCGT CGGCGCCGAC CCGGACGAGC TGGTGTTCAC CAAGAACGCC ACCGAGGCGC TCAACCTGGT GTCGTACGTG GTGGGGGACA ACCGTTTCGA GCGGGCCGTC GGCCCCGGTG ACGTGATCGT CACCACCGAG CTGGAGCATC ACGCCAACCT GGTGCCGTGG CAGGAGTTGG CCCGGCGCAC CGGGGCCAGC CTGCGCTGGT ACGGCGTCAC CGACGACGGG CGGATCGACC TGGACTCGTT GCAGCTCGAC GAGCGGGTCA AAGTGGTTGC CTTCAGCCAT CATTCGAACG TGACCGGGGC GGTGGCGCCG GTGGCCGAGC TGGTGGGGCG GGCCCGCGCG GTGGGCGCGC TGACGGTGCT GGACGCCTGT CAGTCGGTGC CGCACCAGCC GGTGGACTTC CACGCGCTCG ACGTCGACTT CGCCGCGTTC TCCGGGCACA AGATGTTGGG CCCCAACGGG ATCGGCGTGC TCTACGGGCG CCGCGAGGTG TTGTCGCAGC TGCCGCCGTT CCTCACCGGT GGCTCGATGA TCGAGACCGT GACCATGGAG TCCTCCAGCT ACGCGCCGGT GCCGCAGCGG TTCGAGGCCG GCACCCCGAT GGCCTCCCAG GTGGTCGGGT TGGCCGCGGC CGCCCGCTAT CTCGGCGCCC TCGGCATGGA CGCCGTGCAG GCCCACGAGG AGCGGCTGGT CGCCGCGGCC ATCGAGGGCC TGTCCGGTAT CGACGCGGTC CGCATCGTCG GGCCGACGTC GACCGAAAAC CGTGGCTCGC CGGTGTCTTT CGTCGTCGAC GGGGTGCACG CGCACGATGT CGGGCAGGTG CTCGACGACG AGGGCGTGGC GGTGCGGGTG GGCCACCACT GCGCGCTGCC GCTGCACCGC CGGTTCGGAT TGGCGGCCAC CGCCCGGGCG TCGTTCGCGG TGTACAACAC CGCCGAGGAG GTCGACCGCC TGGTGGCGGG TGTGCGCCGC GCCCTGGACT TCTTCGGCGG AGCG
Details for MyavA.00304.a.A1.PS00583
PURIFICATION DATe: 5/2/2010
CONCENTRATION: 16.52mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MVAAIRADFP ILKRIMRSGN QLAYLDSGAT SQRPLQVLDA EREFLLTSNG AVHRGAHQLM EESTDAYEQG RADIAAFVGA DPDELVFTKN ATEALNLVSY VVGDNRFERA VGPGDVIVTT ELEHHANLVP WQELARRTGA SLRWYGVTDD GRIDLDSLQL DERVKVVAFS HHSNVTGAVA PVAELVGRAR AVGALTVLDA CQSVPHQPVD FHALDVDFAA FSGHKMLGPN GIGVLYGRRE VLSQLPPFLT GGSMIETVTM ESSSYAPVPQ RFEAGTPMAS QVVGLAAAAR YLGALGMDAV QAHEERLVAA AIEGLSGIDA VRIVGPTSTE NRGSPVSFVV DGVHAHDVGQ VLDDEGVAVR VGHHCALPLH RRFGLAATAR ASFAVYNTAE EVDRLVAGVR RALDFFGGA
Validated NT Sequence
ctcagaccca gggtcctggt tcgatggtgg ccgcgatccg cgccgatttc cccattctga aacggatcat gcgcagcgga aaccagttgg cgtacctgga ttccggtgcg acgtcgcaac gcccactgca ggtgctcgac gccgagcggg aattcctgct gacctccaac ggcgccgtgc accgcggtgc gcaccagctg atggaggagt ccaccgacgc ctacgagcag ggccgcgccg acatcgcggc gttcgtcggc gccgacccgg acgagctggt gttcaccaag aacgccaccg aggcgctcaa cctggtgtcg tacgtggtgg gggacaaccg tttcgagcgg gccgtcggcc ccggtgacgt gatcgtcacc accgagctgg agcatcacgc caacctggtg ccgtggcagg agttggcccg gcgcaccggg gccagcctgc gctggtacgg cgtcaccgac gacgggcgga tcgacctgga ctcgttgcag ctcgacgagc gggtcaaagt ggttgccttc agccatcatt cgaacgtgac cggggcggtg gcgccggtgg ccgagctggt ggggcgggcc cgcgcggtgg gcgcgctgac ggtgctggac gcctgtcagt cggtgccgca ccagccggtg gacttccacg cgctcgacgt cgacttcgcc gcgttctccg ggcacaagat gttgggcccc aacgggatcg gcgtgctcta cgggcgccgc gaggtgttgt cgcagctgcc gccgttcctc accggtggct cgatgatcga gaccgtgacc atggagtcct ccagctacgc gccggtgccg cagcggttcg aggccggcac cccgatggcc tcccaggtgg tcgggttggc cgcggccgcc cgctatctcg gcgccctcgg catggacgcc gtgcaggccc acgaggagcg gctggtcgcc gcggccatcg agggcctgtc cggtatcgac gcggtccgca tcgtcgggcc gacgtcgacc gaaaaccgtg gctcgccggt gtctttcgtc gtcgacgggg tgcacgcgca cgatgtcggg caggtgctcg acgacgaggg cgtggcggtg cgggtgggcc accactgcgc gctgccgctg caccgccggt tcggattggc ggccaccgcc cgggcgtcgt tcgcggtgta caacaccgcc gaggaggtcg accgcctggt ggcgggtgtg cgccgcgccc tggacttctt cggcggagcg taat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAAIRADF PILKRIMRSG NQLAYLDSGA TSQRPLQVLD AEREFLLTSN GAVHRGAHQL MEESTDAYEQ GRADIAAFVG ADPDELVFTK NATEALNLVS YVVGDNRFER AVGPGDVIVT TELEHHANLV PWQELARRTG ASLRWYGVTD DGRIDLDSLQ LDERVKVVAF SHHSNVTGAV APVAELVGRA RAVGALTVLD ACQSVPHQPV DFHALDVDFA AFSGHKMLGP NGIGVLYGRR EVLSQLPPFL TGGSMIETVT MESSSYAPVP QRFEAGTPMA SQVVGLAAAA RYLGALGMDA VQAHEERLVA AAIEGLSGID AVRIVGPTST ENRGSPVSFV VDGVHAHDVG QVLDDEGVAV RVGHHCALPL HRRFGLAATA RASFAVYNTA EEVDRLVAGV RRALDFFGGA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCCGCG ATCCGCGCCG ATTTCCCCAT TCTGAAACGG ATCATGCGCA GCGGAAACCA GTTGGCGTAC CTGGATTCCG GTGCGACGTC GCAACGCCCA CTGCAGGTGC TCGACGCCGA GCGGGAATTC CTGCTGACCT CCAACGGCGC CGTGCACCGC GGTGCGCACC AGCTGATGGA GGAGTCCACC GACGCCTACG AGCAGGGCCG CGCCGACATC GCGGCGTTCG TCGGCGCCGA CCCGGACGAG CTGGTGTTCA CCAAGAACGC CACCGAGGCG CTCAACCTGG TGTCGTACGT GGTGGGGGAC AACCGTTTCG AGCGGGCCGT CGGCCCCGGT GACGTGATCG TCACCACCGA GCTGGAGCAT CACGCCAACC TGGTGCCGTG GCAGGAGTTG GCCCGGCGCA CCGGGGCCAG CCTGCGCTGG TACGGCGTCA CCGACGACGG GCGGATCGAC CTGGACTCGT TGCAGCTCGA CGAGCGGGTC AAAGTGGTTG CCTTCAGCCA TCATTCGAAC GTGACCGGGG CGGTGGCGCC GGTGGCCGAG CTGGTGGGGC GGGCCCGCGC GGTGGGCGCG CTGACGGTGC TGGACGCCTG TCAGTCGGTG CCGCACCAGC CGGTGGACTT CCACGCGCTC GACGTCGACT TCGCCGCGTT CTCCGGGCAC AAGATGTTGG GCCCCAACGG GATCGGCGTG CTCTACGGGC GCCGCGAGGT GTTGTCGCAG CTGCCGCCGT TCCTCACCGG TGGCTCGATG ATCGAGACCG TGACCATGGA GTCCTCCAGC TACGCGCCGG TGCCGCAGCG GTTCGAGGCC GGCACCCCGA TGGCCTCCCA GGTGGTCGGG TTGGCCGCGG CCGCCCGCTA TCTCGGCGCC CTCGGCATGG ACGCCGTGCA GGCCCACGAG GAGCGGCTGG TCGCCGCGGC CATCGAGGGC CTGTCCGGTA TCGACGCGGT CCGCATCGTC GGGCCGACGT CGACCGAAAA CCGTGGCTCG CCGGTGTCTT TCGTCGTCGA CGGGGTGCAC GCGCACGATG TCGGGCAGGT GCTCGACGAC GAGGGCGTGG CGGTGCGGGT GGGCCACCAC TGCGCGCTGC CGCTGCACCG CCGGTTCGGA TTGGCGGCCA CCGCCCGGGC GTCGTTCGCG GTGTACAACA CCGCCGAGGA GGTCGACCGC CTGGTGGCGG GTGTGCGCCG CGCCCTGGAC TTCTTCGGCG GAGCGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA