MyavA.00272.a

Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase

CENTER ID: MyavA.00272.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00272.a.A1.GE28500 Full length( MyavA.00272.a ) 1 381
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.3137
RefSeq: YP_882387.1
UniProt: A0QHJ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKAPIEVSPL AWLDAVEQQR RAAGLRRSLR PRPPVATELD LASNDYLGLS QHPDVIDGGV AALRLWGAGA TGSRLVTGDT ELHQQFESEL ADYVGAASGL LFSSGYAANL GAVVGLSGRG ALVVSDAYSH ASLVDACRLS RARVVVTPHR DVDAVLAALA DRDEERAVVI TESVFSTDGA LAPLRELHEV CRRHRALLIV DEAHGLGVRG GGRGLVFEAG LAGAPDVVMT TTLSKALGSQ GGAVLGPAAV RAHLIDAART FIFDTGLAPA AVGAARAALG VLRAEPWRVG AVLRHAGVLA EVCRVREVPQ SAVVSVILGD PDVAVAAATA CLDAGVRVGC FRPPTVPAGT SRLRLTARAS LDDAELEVAR RVLTDVLAGL G
NT Sequence
ATGAAAGCAC CCATCGAGGT TTCCCCGCTG GCCTGGCTGG ACGCGGTCGA ACAGCAGCGC CGCGCGGCCG GGCTGCGCCG CTCGCTGCGG CCGCGGCCGC CCGTGGCCAC CGAGCTGGAC CTGGCCTCCA ACGACTACCT CGGGCTGTCC CAGCACCCCG ACGTGATCGA CGGCGGGGTC GCCGCGCTGC GCCTGTGGGG CGCCGGGGCG ACCGGGTCGC GGCTGGTCAC CGGCGATACC GAGCTGCACC AGCAGTTCGA GTCCGAGCTC GCCGACTACG TCGGCGCGGC CAGCGGGCTG CTGTTCTCGT CGGGCTACGC GGCCAACCTT GGGGCCGTCG TGGGCCTGTC GGGCCGCGGG GCGCTGGTGG TCTCCGACGC GTACTCGCAC GCCTCGCTGG TGGACGCCTG CCGGCTGTCG CGGGCGCGGG TGGTAGTGAC GCCGCATCGC GACGTCGACG CGGTGCTGGC GGCGCTGGCG GACCGCGACG AGGAACGCGC CGTCGTCATC ACCGAGTCGG TGTTCTCCAC CGACGGCGCC CTGGCGCCGC TGCGCGAGCT GCACGAGGTG TGCCGCCGGC ACCGGGCCCT GCTGATCGTC GACGAGGCGC ACGGCCTGGG GGTGCGCGGC GGCGGGCGCG GGCTGGTGTT CGAGGCCGGC CTGGCCGGCG CGCCCGACGT GGTCATGACC ACCACGCTGT CCAAGGCGCT GGGCAGCCAG GGCGGCGCGG TGCTGGGGCC GGCGGCGGTG CGGGCCCATC TGATCGACGC GGCGCGCACG TTCATCTTCG ACACCGGCCT GGCCCCGGCG GCGGTGGGCG CGGCGCGCGC CGCGCTGGGC GTGCTGCGCG CCGAACCCTG GCGGGTCGGT GCGGTGCTGC GCCACGCCGG CGTGCTGGCC GAGGTCTGCC GGGTTCGCGA GGTGCCGCAG TCGGCGGTGG TGTCGGTGAT CCTGGGCGAC CCGGACGTGG CGGTGGCCGC CGCGACCGCC TGCCTGGATG CCGGTGTGCG GGTGGGCTGC TTTCGTCCCC CGACGGTGCC CGCCGGGACG TCGCGGCTGC GGCTGACCGC CCGCGCGTCG CTGGACGACG CCGAACTGGA GGTCGCGCGC CGGGTGCTCA CCGATGTGCT CGCCGGGCTC GGC
Details for MyavA.00272.a.A1.GE28500
HARVESTED ON: 2/9/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKAPIEVSP LAWLDAVEQQ RRAAGLRRSL RPRPPVATEL DLASNDYLGL SQHPDVIDGG VAALRLWGAG ATGSRLVTGD TELHQQFESE LADYVGAASG LLFSSGYAAN LGAVVGLSGR GALVVSDAYS HASLVDACRL SRARVVVTPH RDVDAVLAAL ADRDEERAVV ITESVFSTDG ALAPLRELHE VCRRHRALLI VDEAHGLGVR GGGRGLVFEA GLAGAPDVVM TTTLSKALGS QGGAVLGPAA VRAHLIDAAR TFIFDTGLAP AAVGAARAAL GVLRAEPWRV GAVLRHAGVL AEVCRVREVP QSAVVSVILG DPDVAVAAAT ACLDAGVRVG CFRPPTVPAG TSRLRLTARA SLDDAELEVA RRVLTDVLAG LG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaagcaccc atcgaggttt ccccgctggc ctggctggac gcggtcgaac agcagcgccg cgcggccggg ctgcgccgct cgctgcggcc gcggccgccc gtggccaccg agctggacct ggcctccaac gactacctcg ggctgtccca gcaccccgac gtgatcgacg gcggggtcgc cgcgctgcgc ctgtggggcg ccggggcgac cgggtcgcgg ctggtcaccg gcgataccga gctgcaccag cagttcgagt ccgagctcgc cgactacgtc ggcgcggcca gcgggctgct gttctcgtcg ggctacgcgg ccaaccttgg ggccgtcgtg ggcctgtcgg gccgcggggc gctggtggtc tccgacgcgt actcgcacgc ctcgctggtg gacgcctgcc ggctgtcgcg ggcgcgggtg gtagtgacgc cgcatcgcga cgtcgacgcg gtgctggcgg cgctggcgga ccgcgacgag gaacgcgccg tcgtcatcac cgagtcggtg ttctccaccg acggcgccct ggcgccgctg cgcgagctgc acgaggtgtg ccgccggcac cgggccctgc tgatcgtcga cgaggcgcac ggcctggggg tgcgcggcgg cgggcgcggg ctggtgttcg aggccggcct ggccggcgcg cccgacgtgg tcatgaccac cacgctgtcc aaggcgctgg gcagccaggg cggcgcggtg ctggggccgg cggcggtgcg ggcccatctg atcgacgcgg cgcgcacgtt catcttcgac accggcctgg ccccggcggc ggtgggcgcg gcgcgcgccg cgctgggcgt gctgcgcgcc gaaccctggc gggtcggtgc ggtgctgcgc cacgccggcg tgctggccga ggtctgccgg gttcgcgagg tgccgcagtc ggcggtggtg tcggtgatcc tgggcgaccc ggacgtggcg gtggccgccg cgaccgcctg cctggatgcc ggtgtgcggg tgggctgctt tcgtcccccg acggtgcccg ccgggacgtc gcggctgcgg ctgaccgccc gcgcgtcgct ggacgacgcc gaactggagg tcgcgcgccg ggtgctcacc gatgtgctcg ccgggctcgg caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa