MyavA.00250.a
NADH-fmn oxidoreductase
| CENTER ID: | MyavA.00250.a |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.00250.a.A1.GE28498 | Full length( MyavA.00250.a ) | 1 | 162 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4YWN
DEPOSITED: 3/20/2015
DETERMINATION: XRay
CLONE: MyavA.00250.a.A1.GE28498
PROTEIN: MyavA.00250.a.A1.PS00591
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|243243.7.peg.4826 |
| RefSeq: | YP_884033.1 |
| UniProt: | A0A0H2ZXI9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNSNNKLTAS SLREAFGHFP SGVVAIAAEV NGTLEGLAAS TFVPVSLDPP LVSFCVQNTS TTWPKLKDRP MLGISVLGEE HDEAARTLAA KTGDRFAGLE TVSRPTGAVF IKGTALWLES AVEQLIPAGD HTIVVLRVNE VTVDANVAPI VFHRSGFRRL GA
NT Sequence
GTGAATTCGA ACAACAAGCT GACAGCGTCG TCACTTCGTG AGGCCTTCGG TCATTTCCCG TCCGGTGTGG TGGCGATCGC CGCCGAGGTG AACGGAACCC TGGAGGGCCT GGCGGCCAGC ACCTTCGTCC CGGTCTCGCT GGACCCGCCG CTGGTGTCGT TCTGCGTGCA GAACACCTCG ACCACCTGGC CCAAGCTCAA GGACCGGCCC ATGCTGGGGA TCAGCGTGCT CGGCGAGGAG CACGACGAGG CCGCCCGCAC GCTGGCCGCC AAGACCGGCG ACCGGTTCGC CGGACTGGAG ACCGTCTCGC GGCCCACCGG CGCGGTGTTC ATCAAGGGCA CCGCCCTGTG GCTGGAAAGC GCGGTTGAAC AGCTGATCCC GGCCGGGGAT CACACCATCG TGGTGTTGCG GGTCAACGAG GTGACCGTGG ACGCCAACGT GGCGCCCATC GTGTTTCATC GCAGCGGGTT CCGCCGCCTC GGCGCC
Details for MyavA.00250.a.A1.GE28498
| HARVESTED ON: | 2/9/2010 |
| SEQUENCED ON: | 2/22/2010 |
| EXPECTED MW: | 19kDa |
| OBSERVED MW: | 18kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVNSNNKLT ASSLREAFGH FPSGVVAIAA EVNGTLEGLA ASTFVPVSLD PPLVSFCVQN TSTTWPKLKD RPMLGISVLG EEHDEAARTL AAKTGDRFAG LETVSRPTGA VFIKGTALWL ESAVEQLIPA GDHTIVVLRV NEVTVDANVA PIVFHRSGFR RLGA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga attcgaacaa caagctgaca gcgtcgtcac ttcgtgaggc cttcggtcat ttcccgtccg gtgtggtggc gatcgccgcc gaggtgaacg gaaccctgga gggcctggcg gccagcacct tcgtcccggt ctcgctggac ccgccgctgg tgtcgttctg cgtgcagaac acctcgacca cctggcccaa gctcaaggac cggcccatgc tggggatcag cgtgctcggc gaggagcacg acgaggccgc ccgcacgctg gccgccaaga ccggcgaccg gttcgccgga ctggagaccg tctcgcggcc caccggcgcg gtgttcatca agggcaccgc cctgtggctg gaaagcgcgg ttgaacagct gatcccggcc ggggatcaca ccatcgtggt gttgcgggtc aacgaggtga ccgtggacgc caacgtggcg cccatcgtgt ttcatcgcag cgggttccgc cgcctcggcg cctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNSNNKLT ASSLREAFGH FPSGVVAIAA EVNGTLEGLA ASTFVPVSLD PPLVSFCVQN TSTTWPKLKD RPMLGISVLG EEHDEAARTL AAKTGDRFAG LETVSRPTGA VFIKGTALWL ESAVEQLIPA GDHTIVVLRV NEVTVDANVA PIVFHRSGFR RLGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaattcg aacaacaagc tgacagcgtc gtcacttcgt gaggccttcg gtcatttccc gtccggtgtg gtggcgatcg ccgccgaggt gaacggaacc ctggagggcc tggcggccag caccttcgtc ccggtctcgc tggacccgcc gctggtgtcg ttctgcgtgc agaacacctc gaccacctgg cccaagctca aggaccggcc catgctgggg atcagcgtgc tcggcgagga gcacgacgag gccgcccgca cgctggccgc caagaccggc gaccggttcg ccggactgga gaccgtctcg cggcccaccg gcgcggtgtt catcaagggc accgccctgt ggctggaaag cgcggttgaa cagctgatcc cggccgggga tcacaccatc gtggtgttgc gggtcaacga ggtgaccgtg gacgccaacg tggcgcccat cgtgtttcat cgcagcgggt tccgccgcct cggcgccaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa