SSGCID: Target Page

MyavA.00247.h

Putative acyl-CoA dehydrogenase

CENTER ID:	MyavA.00247.h
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00247.h.A1.GE28494	Full length( MyavA.00247.h )		1	391

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00247.h.A1.PS00619	Full length( MyavA.00247.h )		1	391

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.2808
RefSeq:	YP_882061.1
UniProt:	A0A0H2ZSW7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTLSAATTA DIDHYRTVLA GAFDDQVLEW TREAEARQRF PRELIEHLGA RGVFSEKWCG GMLPDVGKLV ELARALGRLS SAGIGVGVSL HDSAIAVLRR FGKSDYLRDI CERAIAGQAV LCIGASEESG GSDLQIVRTE MSSRDGGFDV RGVKKFVSLS PIADHIMVVA RSIDHDSASK HGNVALIAVP TSQVSVQRPY AKVGAGPLDT AAVHIDTWVP ADALVARAGT GLAAISWGLA HERMSIAGQI AASCQRAIGI TLARMMTRRQ FGRTLFEHQA LRLRMADLQA RVDLLQHGLN GIAAQGRLDL RAAAGVKVTA ARLGEEVMSE CMHIFGGAGY LVEETPLGRW WRDMKLARVG GGTDEVLWEL VAAGMAADHG GYRSVVGASS A

NT Sequence

GTGACGACCC TGTCCGCGGC AACAACGGCC GACATCGACC ACTACCGGAC CGTTCTCGCC GGCGCCTTCG ACGATCAGGT GCTGGAGTGG ACGCGCGAAG CCGAAGCGCG GCAACGGTTT CCGCGCGAGC TGATCGAACA CCTGGGCGCC CGCGGTGTGT TCAGCGAAAA GTGGTGCGGC GGAATGCTTC CCGACGTCGG CAAGCTCGTC GAACTTGCCC GAGCGCTCGG CCGGCTGTCG TCGGCGGGCA TCGGGGTGGG GGTCAGCCTG CACGACTCGG CGATCGCCGT CCTGCGCCGG TTCGGCAAGT CGGACTACCT GCGCGACATC TGCGAGCGGG CAATCGCGGG GCAGGCCGTG CTGTGCATCG GGGCCTCCGA GGAGTCCGGC GGCTCCGATC TGCAGATCGT GCGCACCGAG ATGTCTTCCC GCGACGGCGG TTTCGACGTC CGCGGCGTCA AGAAATTCGT CTCCCTGTCC CCCATCGCCG ATCACATCAT GGTGGTGGCG CGCAGCATCG ACCACGACTC GGCCAGCAAA CACGGCAACG TCGCGCTGAT CGCGGTCCCG ACCTCCCAGG TCAGCGTGCA GCGACCCTAT GCCAAGGTCG GCGCCGGACC GCTGGACACC GCCGCGGTGC ACATCGACAC CTGGGTACCC GCTGACGCGC TGGTCGCCCG GGCGGGCACC GGGCTGGCGG CCATCAGCTG GGGGCTGGCG CACGAGCGGA TGTCGATCGC GGGGCAGATC GCCGCGTCGT GCCAGCGGGC CATCGGTATC ACGTTGGCCC GCATGATGAC TCGCCGGCAA TTCGGCCGCA CGCTGTTCGA GCACCAGGCG CTGCGGCTGC GGATGGCCGA CCTGCAGGCC CGCGTCGACC TGTTGCAGCA CGGGCTGAAC GGCATCGCCG CACAGGGCCG GCTGGACCTG CGCGCCGCCG CCGGCGTCAA GGTGACCGCG GCCCGGCTCG GCGAGGAGGT GATGTCGGAG TGCATGCACA TCTTCGGCGG GGCAGGCTAT CTGGTCGAGG AGACACCGCT CGGGCGGTGG TGGCGCGACA TGAAGCTGGC CCGGGTCGGC GGCGGCACCG ACGAGGTGTT GTGGGAGTTG GTCGCCGCCG GCATGGCGGC CGACCATGGC GGCTATCGGT CCGTGGTGGG GGCGTCCAGC GCG

Details for MyavA.00247.h.A1.GE28494

HARVESTED ON:	2/9/2010
SEQUENCED ON:	2/22/2010
EXPECTED MW:	44kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG LMVTTLSAAT TADIDHYRTV LAGAFDDQVL EWTREAEARQ RFPRELIEHL GARGVFSEKW CGGMLPDVGK LVELARALGR LSSAGIGVGV SLHDSAIAVL RRFGKSDYLR DICERAIAGQ AVLCIGASEE SGGSDLQIVR TEMSSRDGGF DVRGVKKFVS LSPIADHIMV VARSIDHDSA SKHGNVALIA VPTSQVSVQR PYAKVGAGPL DTAAVHIDTW VPADALVARA GTGLAAISWG LAHERMSIAG QIAASCQRAI GITLARMMTR RQFGRTLFEH QALRLRMADL QARVDLLQHG LNGIAAQGRL DLRAAAGVKV TAARLGEEVM SECMHIFGGA GYLVEETPLG RWWRDMKLAR VGGGTDEVLW ELVAAGMAAD HGGYRSVVGA SSA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt ttgatggtga cgaccctgtc cgcggcaaca acggccgaca tcgaccacta ccggaccgtt ctcgccggcg ccttcgacga tcaggtgctg gagtggacgc gcgaagccga agcgcggcaa cggtttccgc gcgagctgat cgaacacctg ggcgcccgcg gtgtgttcag cgaaaagtgg tgcggcggaa tgcttcccga cgtcggcaag ctcgtcgaac ttgcccgagc gctcggccgg ctgtcgtcgg cgggcatcgg ggtgggggtc agcctgcacg actcggcgat cgccgtcctg cgccggttcg gcaagtcgga ctacctgcgc gacatctgcg agcgggcaat cgcggggcag gccgtgctgt gcatcggggc ctccgaggag tccggcggct ccgatctgca gatcgtgcgc accgagatgt cttcccgcga cggcggtttc gacgtccgcg gcgtcaagaa attcgtctcc ctgtccccca tcgccgatca catcatggtg gtggcgcgca gcatcgacca cgactcggcc agcaaacacg gcaacgtcgc gctgatcgcg gtcccgacct cccaggtcag cgtgcagcga ccctatgcca aggtcggcgc cggaccgctg gacaccgccg cggtgcacat cgacacctgg gtacccgctg acgcgctggt cgcccgggcg ggcaccgggc tggcggccat cagctggggg ctggcgcacg agcggatgtc gatcgcgggg cagatcgccg cgtcgtgcca gcgggccatc ggtatcacgt tggcccgcat gatgactcgc cggcaattcg gccgcacgct gttcgagcac caggcgctgc ggctgcggat ggccgacctg caggcccgcg tcgacctgtt gcagcacggg ctgaacggca tcgccgcaca gggccggctg gacctgcgcg ccgccgccgg cgtcaaggtg accgcggccc ggctcggcga ggaggtgatg tcggagtgca tgcacatctt cggcggggca ggctatctgg tcgaggagac accgctcggg cggtggtggc gcgacatgaa gctggcccgg gtcggcggcg gcaccgacga ggtgttgtgg gagttggtcg ccgccggcat ggcggccgac catggcggct atcggtccgt ggtgggggcg tccagcgcgt aataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVTTLSAAT TADIDHYRTV LAGAFDDQVL EWTREAEARQ RFPRELIEHL GARGVFSEKW CGGMLPDVGK LVELARALGR LSSAGIGVGV SLHDSAIAVL RRFGKSDYLR DICERAIAGQ AVLCIGASEE SGGSDLQIVR TEMSSRDGGF DVRGVKKFVS LSPIADHIMV VARSIDHDSA SKHGNVALIA VPTSQVSVQR PYAKVGAGPL DTAAVHIDTW VPADALVARA GTGLAAISWG LAHERMSIAG QIAASCQRAI GITLARMMTR RQFGRTLFEH QALRLRMADL QARVDLLQHG LNGIAAQGRL DLRAAAGVKV TAARLGEEVM SECMHIFGGA GYLVEETPLG RWWRDMKLAR VGGGTDEVLW ELVAAGMAAD HGGYRSVVGA SSA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacgacc ctgtccgcgg caacaacggc cgacatcgac cactaccgga ccgttctcgc cggcgccttc gacgatcagg tgctggagtg gacgcgcgaa gccgaagcgc ggcaacggtt tccgcgcgag ctgatcgaac acctgggcgc ccgcggtgtg ttcagcgaaa agtggtgcgg cggaatgctt cccgacgtcg gcaagctcgt cgaacttgcc cgagcgctcg gccggctgtc gtcggcgggc atcggggtgg gggtcagcct gcacgactcg gcgatcgccg tcctgcgccg gttcggcaag tcggactacc tgcgcgacat ctgcgagcgg gcaatcgcgg ggcaggccgt gctgtgcatc ggggcctccg aggagtccgg cggctccgat ctgcagatcg tgcgcaccga gatgtcttcc cgcgacggcg gtttcgacgt ccgcggcgtc aagaaattcg tctccctgtc ccccatcgcc gatcacatca tggtggtggc gcgcagcatc gaccacgact cggccagcaa acacggcaac gtcgcgctga tcgcggtccc gacctcccag gtcagcgtgc agcgacccta tgccaaggtc ggcgccggac cgctggacac cgccgcggtg cacatcgaca cctgggtacc cgctgacgcg ctggtcgccc gggcgggcac cgggctggcg gccatcagct gggggctggc gcacgagcgg atgtcgatcg cggggcagat cgccgcgtcg tgccagcggg ccatcggtat cacgttggcc cgcatgatga ctcgccggca attcggccgc acgctgttcg agcaccaggc gctgcggctg cggatggccg acctgcaggc ccgcgtcgac ctgttgcagc acgggctgaa cggcatcgcc gcacagggcc ggctggacct gcgcgccgcc gccggcgtca aggtgaccgc ggcccggctc ggcgaggagg tgatgtcgga gtgcatgcac atcttcggcg gggcaggcta tctggtcgag gagacaccgc tcgggcggtg gtggcgcgac atgaagctgg cccgggtcgg cggcggcacc gacgaggtgt tgtgggagtt ggtcgccgcc ggcatggcgg ccgaccatgg cggctatcgg tccgtggtgg gggcgtccag cgcgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for MyavA.00247.h.A1.PS00619

PURIFICATION DATe:	6/1/2010
CONCENTRATION:	4.99mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	15µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACGACC CTGTCCGCGG CAACAACGGC CGACATCGAC CACTACCGGA CCGTTCTCGC CGGCGCCTTC GACGATCAGG TGCTGGAGTG GACGCGCGAA GCCGAAGCGC GGCAACGGTT TCCGCGCGAG CTGATCGAAC ACCTGGGCGC CCGCGGTGTG TTCAGCGAAA AGTGGTGCGG CGGAATGCTT CCCGACGTCG GCAAGCTCGT CGAACTTGCC CGAGCGCTCG GCCGGCTGTC GTCGGCGGGC ATCGGGGTGG GGGTCAGCCT GCACGACTCG GCGATCGCCG TCCTGCGCCG GTTCGGCAAG TCGGACTACC TGCGCGACAT CTGCGAGCGG GCAATCGCGG GGCAGGCCGT GCTGTGCATC GGGGCCTCCG AGGAGTCCGG CGGCTCCGAT CTGCAGATCG TGCGCACCGA GATGTCTTCC CGCGACGGCG GTTTCGACGT CCGCGGCGTC AAGAAATTCG TCTCCCTGTC CCCCATCGCC GATCACATCA TGGTGGTGGC GCGCAGCATC GACCACGACT CGGCCAGCAA ACACGGCAAC GTCGCGCTGA TCGCGGTCCC GACCTCCCAG GTCAGCGTGC AGCGACCCTA TGCCAAGGTC GGCGCCGGAC CGCTGGACAC CGCCGCGGTG CACATCGACA CCTGGGTACC CGCTGACGCG CTGGTCGCCC GGGCGGGCAC CGGGCTGGCG GCCATCAGCT GGGGGCTGGC GCACGAGCGG ATGTCGATCG CGGGGCAGAT CGCCGCGTCG TGCCAGCGGG CCATCGGTAT CACGTTGGCC CGCATGATGA CTCGCCGGCA ATTCGGCCGC ACGCTGTTCG AGCACCAGGC GCTGCGGCTG CGGATGGCCG ACCTGCAGGC CCGCGTCGAC CTGTTGCAGC ACGGGCTGAA CGGCATCGCC GCACAGGGCC GGCTGGACCT GCGCGCCGCC GCCGGCGTCA AGGTGACCGC GGCCCGGCTC GGCGAGGAGG TGATGTCGGA GTGCATGCAC ATCTTCGGCG GGGCAGGCTA TCTGGTCGAG GAGACACCGC TCGGGCGGTG GTGGCGCGAC ATGAAGCTGG CCCGGGTCGG CGGCGGCACC GACGAGGTGT TGTGGGAGTT GGTCGCCGCC GGCATGGCGG CCGACCATGG CGGCTATCGG TCCGTGGTGG GGGCGTCCAG CGCGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA