MyavA.00211.a

Pyrroline-5-carboxylate reductase (Rv0500 ortholog)

CENTER ID: MyavA.00211.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.00211.a.B1.GE37401 full length 1 295
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.4569
RefSeq: YP_883781.1
UniProt: A0A0H2ZT75

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSGMARIAI IGGGSIGEAL LSGLLRAGRQ VKDLVVVERV PERAKYLADT YSVLMTSVAD AVENASFVVV AVKPADVESV MSEIARAAGQ AESDTAEQVF VTVAAGVTIG YFESRLPAGT PVVRAMPNAA ALVGAGVTAL AKGRFVTAPQ LEGVSALFDS VGGVLSVPES QMDAVTALSG SGPAYFFLLV EALVDAGVAA GLSREVAADL TAQTMAGSAA MLLERMDADR RLGEAETPGM RVDATATQLR ATVTSPGGTT AAGLRELERG GLRAAVDAAV QAAKIRSEQL RITSE
NT Sequence
atgctgtccg gcatggcaag aatcgcgatc atcggtggcg gcagcatcgg ggaggccctg ctgtcgggtc tgctgcgggc gggccggcag gtcaaggacc tggtggtggt cgagcgggtt cccgagcgcg ccaaatatct ggccgacacc tactcggtgc tgatgacctc ggtggccgat gcggtggaaa acgcgtcgtt cgtcgtggtc gcggtgaaac cggccgacgt cgagtcggtg atgtccgaga tcgcccgcgc cgccggccag gccgagagcg acaccgccga gcaggtgttc gtcacggtcg ccgccggggt caccatcggc tacttcgaat cccggctgcc ggccgggaca ccggtggtgc gggccatgcc gaacgcggcc gcgctggtcg gcgccggggt caccgcactg gccaagggcc gcttcgtcac cgccccgcag ctcgagggcg tctcggcgct gttcgactcg gtcgggggcg tgctgagcgt gccggagagc cagatggacg ccgtcaccgc cctgtccggc tccgggccgg cgtatttctt cctgctggtg gaggccctgg tggacgccgg ggtggccgcg ggcctgagcc gcgaggtggc ggccgacctg accgcccaga ccatggccgg ctcggcggcg atgctgctgg agcggatgga cgccgatcgg cgactgggcg aggcggagac gcccgggatg cgggtggacg ccaccgccac ccagctgcgg gccacggtga cctccccggg cggcaccacc gcggccggcc tgcgcgagct ggagcggggg ggcctgcggg cggccgtcga cgccgccgtc caggccgcca aaatacgctc tgagcagcta agaattacat cggagtaa
Details for MyavA.00211.a.B1.GE37401
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MARIAIIGGG SIGEALLSGL LRAGRQVKDL VVVERVPERA KYLADTYSVL MTSVADAVEN ASFVVVAVKP ADVESVMSEI ARAAGQAESD TAEQVFVTVA AGVTIGYFES RLPAGTPVVR AMPNAAALVG AGVTALAKGR FVTAPQLEGV SALFDSVGGV LSVPESQMDA VTALSGSGPA YFFLLVEALV DAGVAAGLSR EVAADLTAQT MAGSAAMLLE RMDADRRLGE AETPGMRVDA TATQLRATVT SPGGTTAAGL RELERGGLRA AVDAAVQAAK IRSEQLRITS E
Validated NT Sequence
atcttactca cttactccga tgtaattctt agctgctcag agcgtatttt ggcggcctgg acggcggcgt cgacggccgc ccgcaggccc ccccgctcca gctcgcgcag gccggccgcg gtggtgccgc ccggggaggt caccgtggcc cgcagctggg tggcggtggc gtccacccgc atcccgggcg tctccgcctc gcccagtcgc cgatcggcgt ccatccgctc cagcagcatc gccgccgagc cggccatggt ctgggcggtc aggtcggccg ccacctcgcg gctcaggccc gcggccaccc cggcgtccac cagggcctcc accagcagga agaaatacgc cggcccggag ccggacaggg cggtgacggc gtccatctgg ctctccggca cgctcagcac gcccccgacc gagtcgaaca gcgccgagac gccctcgagc tgcggggcgg tgacgaagcg gcccttggcc agtgcggtga ccccggcgcc gaccagcgcg gccgcgttcg gcatggcccg caccaccggt gtcccggccg gcagccggga ttcgaagtag ccgatggtga ccccggcggc gaccgtgacg aacacctgct cggcggtgtc gctctcggcc tggccggcgg cgcgggcgat ctcggacatc accgactcga cgtcggccgg tttcaccgcg accacgacga acgacgcgtt ttccaccgca tcggccaccg aggtcatcag caccgagtag gtgtcggcca gatatttggc gcgctcggga acccgctcga ccaccaccag gtccttgacc tgccggcccg cccgcagcag acccgacagc agggcctccc cgatgctgcc gccaccgatg atcgcgattc ttgccatgcc gg
Expected Protein Sequence
MAHHHHHHML SGMARIAIIG GGSIGEALLS GLLRAGRQVK DLVVVERVPE RAKYLADTYS VLMTSVADAV ENASFVVVAV KPADVESVMS EIARAAGQAE SDTAEQVFVT VAAGVTIGYF ESRLPAGTPV VRAMPNAAAL VGAGVTALAK GRFVTAPQLE GVSALFDSVG GVLSVPESQM DAVTALSGSG PAYFFLLVEA LVDAGVAAGL SREVAADLTA QTMAGSAAML LERMDADRRL GEAETPGMRV DATATQLRAT VTSPGGTTAA GLRELERGGL RAAVDAAVQA AKIRSEQLRI TSE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgt ccggcatggc aagaatcgcg atcatcggtg gcggcagcat cggggaggcc ctgctgtcgg gtctgctgcg ggcgggccgg caggtcaagg acctggtggt ggtcgagcgg gttcccgagc gcgccaaata tctggccgac acctactcgg tgctgatgac ctcggtggcc gatgcggtgg aaaacgcgtc gttcgtcgtg gtcgcggtga aaccggccga cgtcgagtcg gtgatgtccg agatcgcccg cgccgccggc caggccgaga gcgacaccgc cgagcaggtg ttcgtcacgg tcgccgccgg ggtcaccatc ggctacttcg aatcccggct gccggccggg acaccggtgg tgcgggccat gccgaacgcg gccgcgctgg tcggcgccgg ggtcaccgca ctggccaagg gccgcttcgt caccgccccg cagctcgagg gcgtctcggc gctgttcgac tcggtcgggg gcgtgctgag cgtgccggag agccagatgg acgccgtcac cgccctgtcc ggctccgggc cggcgtattt cttcctgctg gtggaggccc tggtggacgc cggggtggcc gcgggcctga gccgcgaggt ggcggccgac ctgaccgccc agaccatggc cggctcggcg gcgatgctgc tggagcggat ggacgccgat cggcgactgg gcgaggcgga gacgcccggg atgcgggtgg acgccaccgc cacccagctg cgggccacgg tgacctcccc gggcggcacc accgcggccg gcctgcgcga gctggagcgg gggggcctgc gggcggccgt cgacgccgcc gtccaggccg ccaaaatacg ctctgagcag ctaagaatta catcggagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat