MyavA.00170.a
Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9) (InhA)
| CENTER ID: | MyavA.00170.a |
| ORGANISM: | Mycobacterium avium 104 |
| ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.00170.a.B1.GE42831 | full length | 1 | 269 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyavA.00170.a.B1.PS38585 | full length | 1 | 269 |
Structures
6Q1Y
DEPOSITED: 8/6/2019
DETERMINATION: XRay
CLONE: MyavA.00170.a.B1.GE42831
PROTEIN: MyavA.00170.a.B1.PS38585
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| UniProt: | A0A0H2ZSV5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGLLDGKRI LVTGIITDSS IAFHIAKVAQ EAGAQLVLTG FDRLRLIQRI VDRLPEKAPL IELDVQNEEH LNTLAQRVTA EIGEGNKLDG VVHSIGFMPQ TGMGINPFFD APYEDVSKGI HISAYSYASL AKALLPIMNP GGSIVGMDFD PSRAMPAYNW MTVAKSALES VNRFVAREAG PHGVRSNLVA AGPIRTLAMA GIVGGVLGDQ AAEQIRLLEE GWDQRAPIGW NMKDPTPVAK TVCALLSDWL PATTGTIIYA DGGASTQLL
NT Sequence
atggcaggac tgctcgacgg caaacggatc ctggtcaccg ggatcatcac cgactcgtcg atcgcgtttc acatcgccaa ggtggcccag gaggccgggg cgcagttggt gctgaccggc ttcgaccggc tgcggctgat ccagcgcatc gtcgaccggc tgccggagaa ggccccgttg atcgagctcg acgtgcagaa cgaggagcac ctgaacaccc tggcccagcg ggtgaccgcc gagatcggcg agggcaacaa gctcgacggg gtggtgcact cgatcggctt catgccgcag accggcatgg gcatcaaccc gttcttcgac gcgccgtacg aggatgtctc caaaggcatt cacatctcgg cgtattcgta cgcctcgctg gccaaggcgc tgctgccgat catgaacccc ggcgggtcga tcgtgggcat ggacttcgac cccagccggg cgatgccggc ctacaactgg atgacggtcg cgaagagcgc gctggagtcg gtcaaccggt tcgtggcgcg cgaggccggg ccgcacggcg tgcgctcgaa tctcgttgcg gcgggcccga tccgcacgct ggcgatggcc gggatcgtcg gcggcgtgct cggcgaccag gcggccgagc agatccggct gctggaggag ggctgggatc agcgggcccc gatcggctgg aacatgaagg accccacccc ggtggccaag acggtgtgcg cgctgctgtc cgactggctg ccggccacca ccggcaccat catctacgcc gacggcggcg ccagcaccca attgctgtag
Details for MyavA.00170.a.B1.GE42831
| HARVESTED ON: | 1/9/2019 |
| SEQUENCED ON: | 1/15/2019 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA GLLDGKRILV TGIITDSSIA FHIAKVAQEA GAQLVLTGFD RLRLIQRIVD RLPEKAPLIE LDVQNEEHLN TLAQRVTAEI GEGNRLDGVV HSIGFMPQTG MGINPFFDAP YEDVSKGIHI SAYSYASLAK ALLPIMNPGG SIVGMDFDPS RAMPAYNWMT VAKSALESVN RFVAREAGPH GVRSNLVAAG PIRTLAMAGI VGGVLGDQAA EQIRLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSDWLPA TTGTIIYAXX XXSTQLL
Validated NT Sequence
tcttactcac ttacagcaat tgggtgctgg nnnnnnnnnc ggcgtagatg atggtgccgg tggtggccgg cagccagtcg gacagcagcg cgcacaccgt cttggccacc ggggtggggt ccttcatgtt ccagccgatc ggggcccgct gatcccagcc ctcctccagc agccggatct gctcggccgc ctggtcgccg agcacgccgc cgacgatccc ggccatcgcc agcgtgcgga tcgggcccgc cgcaacgaga ttcgagcgca cgccgtgcgg cccggcctcc cgcgccacga accggttgac cgactccagc gcgctcttcg cgaccgtcat ccagttgtag gccggcatcg cccggctggg gtcgaagtcc atacccacga tcgacccgcc ggggttcatg atcggcagca gcgccttggc cagcgaggcg tacgaatacg ccgagatatg aatgcctttg gagacatcct cgtacggcgc gtcgaagaac gggttgatgc ccatgccggt ctgcggcatg aagccgatcg agtgcaccac cccgtcgagc ctgttgccct cgccgatctc ggcggtcacc cgctgggcca gggtgttcag gtgctcctcg ttctgcacgt cgagctcgat caacggggcc ttctccggca gccggtcgac gatgcgctgg atcagccgca gccggtcgaa gccggtcagc accaactgcg cccccgcctc ctgggccacc ttggcgatgt gaaacgcgat cgacgagtcg gtgatgatcc cggtgaccag gatccgtttg ccgtcgagca gtcctgccat atggtggtgg tggtggtgag ccatgg
Expected Protein Sequence
MAHHHHHHMA GLLDGKRILV TGIITDSSIA FHIAKVAQEA GAQLVLTGFD RLRLIQRIVD RLPEKAPLIE LDVQNEEHLN TLAQRVTAEI GEGNKLDGVV HSIGFMPQTG MGINPFFDAP YEDVSKGIHI SAYSYASLAK ALLPIMNPGG SIVGMDFDPS RAMPAYNWMT VAKSALESVN RFVAREAGPH GVRSNLVAAG PIRTLAMAGI VGGVLGDQAA EQIRLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSDWLPA TTGTIIYADG GASTQLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag gactgctcga cggcaaacgg atcctggtca ccgggatcat caccgactcg tcgatcgcgt ttcacatcgc caaggtggcc caggaggccg gggcgcagtt ggtgctgacc ggcttcgacc ggctgcggct gatccagcgc atcgtcgacc ggctgccgga gaaggccccg ttgatcgagc tcgacgtgca gaacgaggag cacctgaaca ccctggccca gcgggtgacc gccgagatcg gcgagggcaa caagctcgac ggggtggtgc actcgatcgg cttcatgccg cagaccggca tgggcatcaa cccgttcttc gacgcgccgt acgaggatgt ctccaaaggc attcacatct cggcgtattc gtacgcctcg ctggccaagg cgctgctgcc gatcatgaac cccggcgggt cgatcgtggg catggacttc gaccccagcc gggcgatgcc ggcctacaac tggatgacgg tcgcgaagag cgcgctggag tcggtcaacc ggttcgtggc gcgcgaggcc gggccgcacg gcgtgcgctc gaatctcgtt gcggcgggcc cgatccgcac gctggcgatg gccgggatcg tcggcggcgt gctcggcgac caggcggccg agcagatccg gctgctggag gagggctggg atcagcgggc cccgatcggc tggaacatga aggaccccac cccggtggcc aagacggtgt gcgcgctgct gtccgactgg ctgccggcca ccaccggcac catcatctac gccgacggcg gcgccagcac ccaattgctg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for MyavA.00170.a.B1.PS38585
| PURIFICATION DATe: | 5/21/2019 |
| CONCENTRATION: | 20.93mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA GLLDGKRILV TGIITDSSIA FHIAKVAQEA GAQLVLTGFD RLRLIQRIVD RLPEKAPLIE LDVQNEEHLN TLAQRVTAEI GEGNRLDGVV HSIGFMPQTG MGINPFFDAP YEDVSKGIHI SAYSYASLAK ALLPIMNPGG SIVGMDFDPS RAMPAYNWMT VAKSALESVN RFVAREAGPH GVRSNLVAAG PIRTLAMAGI VGGVLGDQAA EQIRLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSDWLPA TTGTIIYAXX XXSTQLL
Validated NT Sequence
tcttactcac ttacagcaat tgggtgctgg nnnnnnnnnc ggcgtagatg atggtgccgg tggtggccgg cagccagtcg gacagcagcg cgcacaccgt cttggccacc ggggtggggt ccttcatgtt ccagccgatc ggggcccgct gatcccagcc ctcctccagc agccggatct gctcggccgc ctggtcgccg agcacgccgc cgacgatccc ggccatcgcc agcgtgcgga tcgggcccgc cgcaacgaga ttcgagcgca cgccgtgcgg cccggcctcc cgcgccacga accggttgac cgactccagc gcgctcttcg cgaccgtcat ccagttgtag gccggcatcg cccggctggg gtcgaagtcc atacccacga tcgacccgcc ggggttcatg atcggcagca gcgccttggc cagcgaggcg tacgaatacg ccgagatatg aatgcctttg gagacatcct cgtacggcgc gtcgaagaac gggttgatgc ccatgccggt ctgcggcatg aagccgatcg agtgcaccac cccgtcgagc ctgttgccct cgccgatctc ggcggtcacc cgctgggcca gggtgttcag gtgctcctcg ttctgcacgt cgagctcgat caacggggcc ttctccggca gccggtcgac gatgcgctgg atcagccgca gccggtcgaa gccggtcagc accaactgcg cccccgcctc ctgggccacc ttggcgatgt gaaacgcgat cgacgagtcg gtgatgatcc cggtgaccag gatccgtttg ccgtcgagca gtcctgccat atggtggtgg tggtggtgag ccatgg
Expressed Protein Sequence
MAHHHHHHMA GLLDGKRILV TGIITDSSIA FHIAKVAQEA GAQLVLTGFD RLRLIQRIVD RLPEKAPLIE LDVQNEEHLN TLAQRVTAEI GEGNKLDGVV HSIGFMPQTG MGINPFFDAP YEDVSKGIHI SAYSYASLAK ALLPIMNPGG SIVGMDFDPS RAMPAYNWMT VAKSALESVN RFVAREAGPH GVRSNLVAAG PIRTLAMAGI VGGVLGDQAA EQIRLLEEGW DQRAPIGWNM KDPTPVAKTV CALLSDWLPA TTGTIIYADG GASTQLL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAG GACTGCTCGA CGGCAAACGG ATCCTGGTCA CCGGGATCAT CACCGACTCG TCGATCGCGT TTCACATCGC CAAGGTGGCC CAGGAGGCCG GGGCGCAGTT GGTGCTGACC GGCTTCGACC GGCTGCGGCT GATCCAGCGC ATCGTCGACC GGCTGCCGGA GAAGGCCCCG TTGATCGAGC TCGACGTGCA GAACGAGGAG CACCTGAACA CCCTGGCCCA GCGGGTGACC GCCGAGATCG GCGAGGGCAA CAAGCTCGAC GGGGTGGTGC ACTCGATCGG CTTCATGCCG CAGACCGGCA TGGGCATCAA CCCGTTCTTC GACGCGCCGT ACGAGGATGT CTCCAAAGGC ATTCACATCT CGGCGTATTC GTACGCCTCG CTGGCCAAGG CGCTGCTGCC GATCATGAAC CCCGGCGGGT CGATCGTGGG CATGGACTTC GACCCCAGCC GGGCGATGCC GGCCTACAAC TGGATGACGG TCGCGAAGAG CGCGCTGGAG TCGGTCAACC GGTTCGTGGC GCGCGAGGCC GGGCCGCACG GCGTGCGCTC GAATCTCGTT GCGGCGGGCC CGATCCGCAC GCTGGCGATG GCCGGGATCG TCGGCGGCGT GCTCGGCGAC CAGGCGGCCG AGCAGATCCG GCTGCTGGAG GAGGGCTGGG ATCAGCGGGC CCCGATCGGC TGGAACATGA AGGACCCCAC CCCGGTGGCC AAGACGGTGT GCGCGCTGCT GTCCGACTGG CTGCCGGCCA CCACCGGCAC CATCATCTAC GCCGACGGCG GCGCCAGCAC CCAATTGCTG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT