SSGCID: Target Page

MyabA.19367.a

Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX [Includes: Lysine--tRNA ligase (EC 6.1.1.6) (Lysyl-tRNA synthetase) (LysRS); Phosphatidylglycerol lysyltransferase (EC 2.3.2.3) (Lysylphosphatidylglycerol synthetase) (LPG synthetase)]

CENTER ID:	MyabA.19367.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.19367.a.B2.GE40722	soluble domain #1		247	1110
MyabA.19367.a.B3.GE40723	soluble domain #2		589	1110

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.2362
UniProt:	B1MAX9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSIKPAVEP PAPDHPAVTA LPGIGARPAA RPPVTSRWRW VPAFFGWFVG IQAILNFLSS ISPFIRMVTK LPREFVDEYL FGWPDGSLSW AFVLSLLAAA LAVRKSIAWW VLLIDLLGRS ALNVGGFIEH RDTTELIGLV VHGAFILLLL LARKEFYAKV RRGATVKAAL VLIAGMAAGT VLAWGLLELF PRTLVPGDRF WWALNRVAGF SIASHRDFDG LPPHFLNWLF GLFGALALIA AAIVLFRSQR AVNALTGDDE SLIRGLLERY GQDDSLGYFA TRRDKAVIFA PNGRAAVTYR VEVGVCLVSA DPIGDKSAWG QAISAWLQVC DAYGWAPAVM GASAEGALAY REHGLNALEL GDEAILYPRS FSISGPHMAT VRQAVNRARR SGLSVRIRRH RELSAEETKQ IIVNADLWRD GNTERGFSMA LGRLGDSTDG DCLLVEAIDS TGDPVAMLSL VPWGTNGVSL ELMRRSRQSP NGTIEFMVTE LLTRAEGMGI TRVSLNFVMF RSVYADGSRI GAGPVLRLWR SVLMFASRFW QFEQLYRSNV KYQPEWVPRF ACYEDARLIP RIGIASVIAE GFLVLPFGNR DKPRHTGQYS AVPQAIVATG LLHHDGSTPE LLPQTAPQQR VPEQVKVRLG KLAALQEKGI DTYPVGSPPS HTVAEALAAP DGSEVVISGR LLRIRDYGGV LFAQLRDWSA EVQLLIDDPR LHEEFTAAVD LGDLVEASGV MGQSNNGTRS VLVESWRIIG KCLHPLPDKR KGLTDPEARV RARYLDLAIN PEAREQIAAR SAIITSLRNT LLAQGFLEVE TPILQQVHGG ANARPFLTHI NAYNLDLYLR IAPELYLKRL CVGGVEKVFE LGRAFRNEGV DFSHNPEFTL LEAYQAHADY VTWAQTTQRL IQNAAKAAHG AEVVMRPGAD GRLAPVDIGG EWPIVPVHEA VSRALGEEVN AATEVPTLQR FCQSAEVQFN PRWDAGHLVL ELYERLVEKH TTSPTFYSDF PVSVSPLTRP HRSIAGLAER WDLVAWGVEL GTAYTELTDP VEQRRRLYEQ SLLAAGGDAE AMELDEDFLR AMEYAMAPTG GMGMGVDRVV MLITGRSIRE TLPFPLAKPR

NT Sequence

gtgacatcca tcaaaccggc agttgaacca cccgccccgg atcatcccgc tgtcacggcg ctcccgggaa tcggtgcacg cccggcagcg cgaccacccg taacctcacg gtggcgctgg gtcccggcgt tcttcggctg gtttgtcggc atccaggcga tcctgaactt cctgtcgagc atctcgccat tcattcggat ggtgacgaag ctgcctcgcg agttcgtcga cgaatacctg ttcggttggc ccgacggcag cctgtcctgg gcttttgtac tgtcgctact ggccgccgcc ctggcggtgc gcaagagcat cgcctggtgg gtgctcttga tcgacctact gggacggtca gcactaaacg tgggcggctt catagagcat cgtgacacca ccgagctgat cggtctcgtc gtgcacggcg cgttcatcct gttgctgctg ctcgcgcgta aggagttcta cgccaaggta cgccgcggtg cgacggtcaa ggcggcgctg gtgctgatcg cggggatggc cgccggaaca gtcctggcat ggggcctgct ggaactcttc ccccgcacgc ttgtccccgg tgatcgtttc tggtgggcac tcaatcgggt ggccggcttt tcgatcgcct cacaccgcga tttcgatggt ctgccccctc acttcctgaa ctggctcttc gggttattcg gcgcgctggc cctgatcgcc gcggccatcg tgttgttccg ctcgcaacga gccgtcaacg cgctgaccgg tgatgacgaa tctctaatcc gcgggctcct ggagcgctat gggcaggacg actccctggg ttatttcgcg acgagacgcg acaaggccgt catcttcgcg cccaatggcc gagcggccgt gacatatcgg gtggaagttg gcgtctgcct ggtcagtgcc gatcccattg gggacaaatc ggcttggggc caagccattt ccgcatggct gcaggtatgc gacgcttacg gctgggcgcc ggcggtcatg ggcgccagcg ccgaaggtgc gctggcgtat cgcgaacatg ggctcaacgc actggaactc ggcgacgagg ccatcttgta cccgcgctca ttctcgattt cgggtccgca tatggccacg gtccggcagg cggtcaaccg cgcacgacgt tcgggactat cggtgcgcat ccgccgccat cgcgaactga gcgccgaaga gacgaaacag atcatcgtca atgccgacct gtggcgtgat ggcaataccg agcgcggctt ctcgatggca ctgggacggt tgggggattc caccgatggc gactgcctgt tggttgaggc gatcgattcc acgggcgatc ccgtcgccat gctgtctctc gtaccgtggg gcaccaacgg ggtgtccctc gaactcatgc gccgctcgcg tcaatcaccc aacggcacta tcgaattcat ggtcacggag ctgctcacac gcgcagaggg tatggggatc acccgcgttt ccctcaactt tgtgatgttt cgctcggtct acgccgacgg ctcccgaatc ggagcgggcc cggtacttcg actgtggcgc tcggtgctga tgttcgcctc acggttctgg cagttcgagc agttatatcg ctccaatgtg aagtatcagc cggaatgggt gccccggttc gcctgctacg aagacgcccg gctcatcccc cgcatcggca tcgcgtcggt gatcgccgaa ggcttcctgg tgctgccctt cggcaaccgc gacaagccga ggcacaccgg ccagtacagc gcggttccac aggccatcgt ggccacgggc ctgctgcatc acgatggcag cacacccgag ctactgcccc agacagcacc tcaacagcgc gtaccggagc aggtcaaggt tcgactgggc aagctggccg ccttacagga gaagggaatc gacacatacc cggtgggatc gccaccgtcc cataccgtgg ccgaggcgct tgccgcaccc gacggtagcg aagtggtcat cagcgggcga ttgttgcgaa tccgcgacta cggcggtgtg ttgttcgcac agctacggga ttggtccgcg gaggtgcagc tgcttatcga cgatccgcgg ctgcacgaag agttcaccgc cgccgtcgat ctgggtgatc tggtcgaggc cagcggggtc atgggtcaat cgaacaacgg cacccgatcg gtcctggtgg agtcgtggcg gatcatcggc aaatgcctgc acccgctccc cgacaagcgc aagggcctta ccgaccccga agccagggtg cgtgcacgat acctcgacct ggcgatcaac cccgaggctc gcgagcagat cgcggcccgt agcgcgatca tcacatcgct gcgcaatacc cttttggctc aaggtttcct agaggtggaa acaccgattc tgcagcaggt gcacggcgga gccaacgcgc gtcccttcct gacacacatc aacgcgtaca acttggatct gtatctgcgt atcgcgccgg agctgtacct caagcggctc tgcgtcggtg gcgtggagaa ggtcttcgaa ttggggcggg ccttccgcaa cgagggcgtc gacttcagcc ataatcccga attcaccctg ctcgaggcct atcaggcgca cgccgactat gtgacctggg cccagaccac gcagcggctc atccagaacg ccgcgaaagc cgcccacggc gccgaagtgg tgatgcggcc cggagccgac gggaggctgg cgccggtgga catcggtggc gaatggccga tcgttcccgt gcatgaggcg gtctctcgcg cgctgggtga agaggtcaac gctgcgacgg aagtgccgac attgcaaagg ttctgccagt ccgcggaggt gcagttcaat ccgcgctggg atgccggtca cctggtcctg gaactctacg aacggctggt ggagaaacac acgacctcac ccacgttcta tagcgatttc ccggtctcgg tgtcgccgct tacccgcccg caccgcagca tcgccgggct cgccgagcgg tgggatctgg tcgcgtgggg tgtcgaactc ggcaccgcct ataccgagtt gaccgacccg gtggaacagc gccgacgcct gtatgagcaa tcactgctgg cggccggcgg agacgccgaa gcgatggaac tcgacgagga tttcctgcgc gcgatggagt acgcaatggc gccaaccgga gggatgggca tgggagtgga ccgggtggtc atgctgatca ccgggcgcag catccgcgaa acactgccct tcccgctggc caagccccgc tag

Details for MyabA.19367.a.B2.GE40722

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	96kDa
OBSERVED MW:	96kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHRS QRAVNALTGD DESLIRGLLE RYGQDDSLGY FATRRDKAVI FAPNGRAAVT YRVEVGVCLV SADPIGDKSA WGQAISAWLQ VCDAYGWAPA VMGASAEGAL AYREHGLNAL ELGDEAILYP RSFSISGPHM ATVRQAVNRA RRSGLSVRIR RHRELSAEET KQIIVNADLW RDGNTERGFS MALGRLGDST DGDCLLVEAI DSTGDPVAML SLVPWGTNGV SLELMRRSRQ SPNGTIEFMV TELLTRAEGM GITRVSLNFV MFRSVYADGS RIGAGPVLRL WRSVLMFASR FWQFEQLYRS NVKYQPEWVP RFACYEDARL IPRIGIASVI AEGFLVLPFG NRDKPRHTGQ YSAVPQAIVA TGLLHHDGST PELLPQTAPQ QRVPEQVKVR LGKLAALQEK GIDTYPVGSP PSHTVAEALA APDGSEVVIS GRLLRIRDYG GVLFAQLRDW SAEVQLLIDD PRLHEEFTAA VDLGDLVEAS GVMGQSNNGT RSVLVESWRI IGKCLHPLPD KRKGLTDPEA RVRARYLDLA INPEAREQIA ARSAIITSLR NTLLAQGFLE VETPILQQVH GGANARPFLT HINAYNLDLY LRIAPELYLK RLCVGGVEKV FELGRAFRNE GVDFSHNPEF TLLEAYQAHA DYVTWAQTTQ RLIQNAAKAA HGAEVVMRPG ADGRLAPVDI GGEWPIVPVH EAVSRALGEE VNAATEVPTL QRFCQSAEVQ FNPRWDAGHL VLELYERLVE KHTTSPTFYS DFPVSVSPLT RPHRSIAGLA ERWDLVAWGV ELGTAYTELT DPVEQRRRLY EQSLLAAGGD AEAMELDEDF LRAMEYAMAP TGGMGMGVDR VVMLITGRSI RETLPFPLAK PR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgctcgc aacgagccgt caacgcgctg accggtgatg acgaatctct aatccgcggg ctcctggagc gctatgggca ggacgactcc ctgggttatt tcgcgacgag acgcgacaag gccgtcatct tcgcgcccaa tggccgagcg gccgtgacat atcgggtgga agttggcgtc tgcctggtca gtgccgatcc cattggggac aaatcggctt ggggccaagc catttccgca tggctgcagg tatgcgacgc ttacggctgg gcgccggcgg tcatgggcgc cagcgccgaa ggtgcgctgg cgtatcgcga acatgggctc aacgcactgg aactcggcga cgaggccatc ttgtacccgc gctcattctc gatttcgggt ccgcatatgg ccacggtccg gcaggcggtc aaccgcgcac gacgttcggg actatcggtg cgcatccgcc gccatcgcga actgagcgcc gaagagacga aacagatcat cgtcaatgcc gacctgtggc gtgatggcaa taccgagcgc ggcttctcga tggcactggg acggttgggg gattccaccg atggcgactg cctgttggtt gaggcgatcg attccacggg cgatcccgtc gccatgctgt ctctcgtacc gtggggcacc aacggggtgt ccctcgaact catgcgccgc tcgcgtcaat cacccaacgg cactatcgaa ttcatggtca cggagctgct cacacgcgca gagggtatgg ggatcacccg cgtttccctc aactttgtga tgtttcgctc ggtctacgcc gacggctccc gaatcggagc gggcccggta cttcgactgt ggcgctcggt gctgatgttc gcctcacggt tctggcagtt cgagcagtta tatcgctcca atgtgaagta tcagccggaa tgggtgcccc ggttcgcctg ctacgaagac gcccggctca tcccccgcat cggcatcgcg tcggtgatcg ccgaaggctt cctggtgctg cccttcggca accgcgacaa gccgaggcac accggccagt acagcgcggt tccacaggcc atcgtggcca cgggcctgct gcatcacgat ggcagcacac ccgagctact gccccagaca gcacctcaac agcgcgtacc ggagcaggtc aaggttcgac tgggcaagct ggccgcctta caggagaagg gaatcgacac atacccggtg ggatcgccac cgtcccatac cgtggccgag gcgcttgccg cacccgacgg tagcgaagtg gtcatcagcg ggcgattgtt gcgaatccgc gactacggcg gtgtgttgtt cgcacagcta cgggattggt ccgcggaggt gcagctgctt atcgacgatc cgcggctgca cgaagagttc accgccgccg tcgatctggg tgatctggtc gaggccagcg gggtcatggg tcaatcgaac aacggcaccc gatcggtcct ggtggagtcg tggcggatca tcggcaaatg cctgcacccg ctccccgaca agcgcaaggg ccttaccgac cccgaagcca gggtgcgtgc acgatacctc gacctggcga tcaaccccga ggctcgcgag cagatcgcgg cccgtagcgc gatcatcaca tcgctgcgca ataccctttt ggctcaaggt ttcctagagg tggaaacacc gattctgcag caggtgcacg gcggagccaa cgcgcgtccc ttcctgacac acatcaacgc gtacaacttg gatctgtatc tgcgtatcgc gccggagctg tacctcaagc ggctctgcgt cggtggcgtg gagaaggtct tcgaattggg gcgggccttc cgcaacgagg gcgtcgactt cagccataat cccgaattca ccctgctcga ggcctatcag gcgcacgccg actatgtgac ctgggcccag accacgcagc ggctcatcca gaacgccgcg aaagccgccc acggcgccga agtggtgatg cggcccggag ccgacgggag gctggcgccg gtggacatcg gtggcgaatg gccgatcgtt cccgtgcatg aggcggtctc tcgcgcgctg ggtgaagagg tcaacgctgc gacggaagtg ccgacattgc aaaggttctg ccagtccgcg gaggtgcagt tcaatccgcg ctgggatgcc ggtcacctgg tcctggaact ctacgaacgg ctggtggaga aacacacgac ctcacccacg ttctatagcg atttcccggt ctcggtgtcg ccgcttaccc gcccgcaccg cagcatcgcc gggctcgccg agcggtggga tctggtcgcg tggggtgtcg aactcggcac cgcctatacc gagttgaccg acccggtgga acagcgccga cgcctgtatg agcaatcact gctggcggcc ggcggagacg ccgaagcgat ggaactcgac gaggatttcc tgcgcgcgat ggagtacgca atggcgccaa ccggagggat gggcatggga gtggaccggg tggtcatgct gatcaccggg cgcagcatcc gcgaaacact gcccttcccg ctggccaagc cccgctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for MyabA.19367.a.B3.GE40723

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	59kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMN RDKPRHTGQY SAVPQAIVAT GLLHHDGSTP ELLPQTAPQQ RVPEQVKVRL GKLAALQEKG IDTYPVGSPP SHTVAEALAA PDGSEVVISG RLLRIRDYGG VLFAQLRDWS AEVQLLIDDP RLHEEFTAAV DLGDLVEASG VMGQSNNGTR SVLVESWRII GKCLHPLPDK RKGLTDPEAR VRARYLDLAI NPEAREQIAA RSAIITSLRN TLLAQGFLEV ETPILXXVHX GXXARPFLTH INAYNXXLYL RIAPELYLKR LCVXGVEKVF ELGRAFRNEG VDFSHNPEFT LLEAYQAHAD YVTWAQTTQR LIQNAAKAAH GAEVVMRPGA DGRLAPVDIG GEWPIVPVHE AVSRALGEEV NAATEVPTLQ RFCQSAEVQF NPRWDAGHLV LELYERLVEK HTTSPTFYSD FPVSVSPLTR PHRSIAGLAE RWDLVAWGVE LGTAYTELTD PVEQRRRLYE QSLLAAGGDA EAMELDEDFL RAMEYAMAPT GGMGMGVDRV VMLITGRSIR ETL

Validated NT Sequence

atggctcacc accaccacca ccatatgaac cgcgacaagc cgaggcacac cggccagtac agcgcggttc cacaggccat cgtggccacg ggcctgctgc atcacgatgg cagcacaccc gagctactgc cccagacagc acctcaacag cgcgtaccgg agcaggtcaa ggttcgactg ggcaagctgg ccgccttaca ggagaaggga atcgacacat acccggtggg atcgccaccg tcccataccg tggccgaggc gcttgccgca cccgacggta gcgaagtggt catcagcggg cgattgttgc gaatccgcga ctacggcggt gtgttgttcg cacagctacg ggattggtcc gcggaggtgc agctgcttat cgacgatccg cggctgcacg aagagttcac cgccgccgtc gatctgggtg atctggtcga ggccagcggg gtcatgggtc aatcgaacaa cggcacccga tcggtcctgg tggagtcgtg gcggatcatc ggcaaatgcc tgcacccgct ccccgacaag cgcaagggcc ttaccgaccc cgaagccagg gtgcgtgcac gatacctcga cctggcgatc aaccccgagg ctcgcgagca gatcgcggcc cgtagcgcga tcatcacatc gctgcgcaat acccttttgg ctcaaggttt cctagaggtg gaaacaccga ttctgcngna ngtgcacnnn ggagnnnnng cgcgtccctt cctgacacac atcaacgcgt acaacttnnn tctgtatctg cgtatcgcgc cggagctgta cctcaagcgg ctctgcgtnn nnggcgtnga gaaggtcttc gaattggggc gggccttccg caacgagggc gtcgacttca gccataatcc cgaattcacc ctgctcgagg cctatcaggc gcacgccgac tatgtgacct gggcccagac cacgcagcgg ctcatccaga acgccgcgaa agccgcccac ggcgccgaag tggtgatgcg gcccggagcc gacgggaggc tggcgccggt ggacatcggt ggcgaatggc cgatcgttcc cgtgcatgag gcggtctctc gcgcgctggg tgaagaggtc aacgctgcga cggaagtgcc gacattgcaa aggttctgcc agtccgcgga ggtgcagttc aatccgcgct gggatgccgg tcacctggtc ctggaactct acgaacggct ggtggagaaa cacacgacct cacccacgtt ctatagcgat ttcccggtct cggtgtcgcc gcttacccgc ccgcaccgca gcatcgccgg gctcgccgag cggtgggatc tggtcgcgtg gggtgtcgaa ctcggcaccg cctataccga gttgaccgac ccggtggaac agcgccgacg cctgtatgag caatcactgc tggcggccgg cggagacgcc gaagcgatgg aactcgacga ggatttcctg cgcgcgatgg agtacgcaat ggcgccaacc ggagggatgg gcatgggagt ggaccgggtg gtcatgctga tcaccgggcg cagcatccgc gaaacactgc cc

Expected Protein Sequence

MAHHHHHHNR DKPRHTGQYS AVPQAIVATG LLHHDGSTPE LLPQTAPQQR VPEQVKVRLG KLAALQEKGI DTYPVGSPPS HTVAEALAAP DGSEVVISGR LLRIRDYGGV LFAQLRDWSA EVQLLIDDPR LHEEFTAAVD LGDLVEASGV MGQSNNGTRS VLVESWRIIG KCLHPLPDKR KGLTDPEARV RARYLDLAIN PEAREQIAAR SAIITSLRNT LLAQGFLEVE TPILQQVHGG ANARPFLTHI NAYNLDLYLR IAPELYLKRL CVGGVEKVFE LGRAFRNEGV DFSHNPEFTL LEAYQAHADY VTWAQTTQRL IQNAAKAAHG AEVVMRPGAD GRLAPVDIGG EWPIVPVHEA VSRALGEEVN AATEVPTLQR FCQSAEVQFN PRWDAGHLVL ELYERLVEKH TTSPTFYSDF PVSVSPLTRP HRSIAGLAER WDLVAWGVEL GTAYTELTDP VEQRRRLYEQ SLLAAGGDAE AMELDEDFLR AMEYAMAPTG GMGMGVDRVV MLITGRSIRE TLPFPLAKPR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataaccgcg acaagccgag gcacaccggc cagtacagcg cggttccaca ggccatcgtg gccacgggcc tgctgcatca cgatggcagc acacccgagc tactgcccca gacagcacct caacagcgcg taccggagca ggtcaaggtt cgactgggca agctggccgc cttacaggag aagggaatcg acacataccc ggtgggatcg ccaccgtccc ataccgtggc cgaggcgctt gccgcacccg acggtagcga agtggtcatc agcgggcgat tgttgcgaat ccgcgactac ggcggtgtgt tgttcgcaca gctacgggat tggtccgcgg aggtgcagct gcttatcgac gatccgcggc tgcacgaaga gttcaccgcc gccgtcgatc tgggtgatct ggtcgaggcc agcggggtca tgggtcaatc gaacaacggc acccgatcgg tcctggtgga gtcgtggcgg atcatcggca aatgcctgca cccgctcccc gacaagcgca agggccttac cgaccccgaa gccagggtgc gtgcacgata cctcgacctg gcgatcaacc ccgaggctcg cgagcagatc gcggcccgta gcgcgatcat cacatcgctg cgcaataccc ttttggctca aggtttccta gaggtggaaa caccgattct gcagcaggtg cacggcggag ccaacgcgcg tcccttcctg acacacatca acgcgtacaa cttggatctg tatctgcgta tcgcgccgga gctgtacctc aagcggctct gcgtcggtgg cgtggagaag gtcttcgaat tggggcgggc cttccgcaac gagggcgtcg acttcagcca taatcccgaa ttcaccctgc tcgaggccta tcaggcgcac gccgactatg tgacctgggc ccagaccacg cagcggctca tccagaacgc cgcgaaagcc gcccacggcg ccgaagtggt gatgcggccc ggagccgacg ggaggctggc gccggtggac atcggtggcg aatggccgat cgttcccgtg catgaggcgg tctctcgcgc gctgggtgaa gaggtcaacg ctgcgacgga agtgccgaca ttgcaaaggt tctgccagtc cgcggaggtg cagttcaatc cgcgctggga tgccggtcac ctggtcctgg aactctacga acggctggtg gagaaacaca cgacctcacc cacgttctat agcgatttcc cggtctcggt gtcgccgctt acccgcccgc accgcagcat cgccgggctc gccgagcggt gggatctggt cgcgtggggt gtcgaactcg gcaccgccta taccgagttg accgacccgg tggaacagcg ccgacgcctg tatgagcaat cactgctggc ggccggcgga gacgccgaag cgatggaact cgacgaggat ttcctgcgcg cgatggagta cgcaatggcg ccaaccggag ggatgggcat gggagtggac cgggtggtca tgctgatcac cgggcgcagc atccgcgaaa cactgccctt cccgctggcc aagccccgct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat