SSGCID: Target Page

MyabA.19295.a

Polyphosphate kinase (EC 2.7.4.1) (ATP-polyphosphate phosphotransferase) (Polyphosphoric acid kinase)

CENTER ID:	MyabA.19295.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.19295.a.B2.GE40721	ordered domain		26	711

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.19295.a.B2.PW38243	ordered domain		26	711

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.3348
UniProt:	B1MDP7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MITRVTTPSV PPAATTIVSG EELPEDRYLN RELSWLDFNA RVLALAADES LPILERAKFL AIFASNLDEF YMVRVAGLKR RDETGLSVRS ADGLSPREQL KLIGERTQEI SVQHARVFSE SVRPELAENG IFVVTWADLS ENERSYLSSY FNDQVFPVLT PLAVDPAHPF PYISGLSLNL AITVQVPETG GQHFARIKVP DNVDRFVRLR SPDGTESPQA AHTARFLPME ELIAAHLSAL FPGLEIVEHH AFRITRNADF EVEEDRDEDL LQALERELAR RRFGSPVRLE VADDMTEHML ELLLRELDVD PGDVVQVPGL LDLSCLWQVY GVDRPALKDP TFVPATHPAF GQGETPKSIF STLRDGDVLV HHPYDSFSTS VQRFIEQAAA DPQVLAIKQT LYRTSGDSPI VNSLIDAAEA GKQVVALVEI KARFDEQANI KWARALEDAG VHVVYGLIGL KTHCKTALVV RREGSAIRRY CHIGTGNYNP KTARLYEDVG LLTSAPEIGA DLTDLFNSLT GYSRKVSYRN LLVAPQGIRA GIVERIDREI AAHRAGQEAR IRLKMNALVD EQVIDALYRA SRAGVPIEVV VRGICALRPG VPEYSGSITV RSILGRFLEH SRIIHFNAID EFWIGSADMM HRNLDRRVEV LARVTDPKLT AQLDDMFTSA LDSHTRCWEL QPDGQWLASP ADGEEVRDHQ VEMMKRHRSQ T

NT Sequence

atgatcacaa gggtgaccac accgagcgtt ccccccgccg caaccaccat cgtgtcgggt gaagagctgc ccgaagaccg gtacctcaac cgggagctct cgtggctgga tttcaacgcg cgtgtgctcg cacttgccgc cgacgaatcg ctgcccatcc tggaacgcgc caaattcctg gcaatcttcg cctccaacct ggatgagttt tacatggtgc gggtcgccgg tctgaaacgc cgtgacgaga cgggcttgtc ggtgcgttcg gccgacggcc tatccccgcg cgaacagctg aaactgatcg gtgagcgcac gcaggagatt tcggtccagc atgctcgggt attcagtgag tcggtgcgcc ccgagctcgc agaaaacggg atctttgtgg tcacctgggc tgacctgtcg gaaaacgagc gcagttatct ttcctcgtat ttcaatgacc aggtcttccc cgtgctcact ccgctggccg tcgaccccgc gcacccgttc ccctacatat cgggcctgag cctgaatctg gcgatcaccg tccaagttcc cgagacaggc ggccagcatt tcgcccgaat caaggtgcct gacaatgtgg atcgttttgt cagactgcgc agccctgacg gcactgagtc accgcaggct gcccataccg cgcgctttct gccgatggag gaactcatcg ccgcgcatct gtcggcgctg ttccccggcc tggaaatcgt cgaacatcac gcgttccgca tcacccggaa cgctgatttc gaggtcgagg aagatcgcga cgaggacctt ctgcaggcac ttgagcggga gctggcgcgc cggcggttcg gctcgccggt gcgccttgag gtcgccgacg acatgaccga gcacatgctc gaactgctac tccgcgagct ggacgtagat cccggtgacg tcgtgcaggt tcccggcctg ctggatctgt cgtgcctgtg gcaggtctac ggcgtggatc ggcccgcctt gaaggatccg acattcgtgc cggcgaccca tccggccttc gggcagggtg aaacccccaa gagcatcttc tccacactgc gggatgggga tgtgctcgtg catcacccgt atgactcgtt ctccaccagc gtgcaacgat ttatcgaaca ggccgccgcg gatcctcagg tgctggccat caagcagacg ctgtatcgca cctccggcga ctctcctatc gtgaactcac tcatcgacgc cgccgaggcc ggtaagcagg ttgtcgccct ggtggagatc aaggcacgct tcgacgaaca ggccaatatc aagtgggcgc gcgcgctgga agacgccggc gtacacgtgg tctacggctt gatcggcctc aagacgcatt gcaagacggc cctggtggtg cgccgggagg gatcggccat ccgcaggtac tgccacatcg gcaccgggaa ctacaacccg aagaccgccc ggctttacga agacgtcggc ctgttgacca gcgcgccgga gattggcgcc gatctcaccg acctgttcaa ctcgctgacc ggatactcgc gaaaggtctc ctatcgcaac ctgttagtgg cgccccaggg catacgcgcc gggatcgtcg aacgcatcga tcgtgagatc gccgcacatc gggccggcca ggaggcccgc atccggctga agatgaatgc gcttgtcgac gagcaggtga tcgatgcgct gtaccgggct tcccgagccg gggtgcccat cgaggtagtg gtacgcggga tctgcgcgct ccgtccaggg gttcctgagt actcgggcag cattaccgtc cgatccattc tcggccggtt cctggaacat tcgcgcatca ttcacttcaa cgccatcgac gaattctgga tcggcagcgc cgacatgatg catcgtaatc tggaccgacg ggtcgaggtc ctcgcacgcg tcaccgatcc gaagctcacc gcacagctcg acgacatgtt cacctcggcg ttggattccc acacccggtg ctgggagctg caacccgacg gccagtggtt ggcctcgccc gcggacggcg aggaggtgcg cgaccatcag gtggagatga tgaaacggca ccgctcgcag acatga

Details for MyabA.19295.a.B2.GE40721

HARVESTED ON:	11/8/2016
SEQUENCED ON:	11/8/2016
EXPECTED MW:	78kDa
OBSERVED MW:	78kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHDR YLNRELSWLD FNARVLALAA DESLPILERA KFLAIFASNL DEFYMVRVAG LKRRDETGLS VRSADGLSPR EQLKLIGERT QEISVQHARV FSESVRPELA ENGIFVVTWA DLSENERSYL SSYFNDQVFP VLTPLAVDPA HPFPYISGLS LNLAITVQVP ETGGQHFARI KVPDNVDRFV RLRSPDGTES PQAAHTARFL PMEELIAAHL SALFPGLEIV EHHAFRITRN ADFEVEEDRD EDLLQALERE LARRRFGSPV RLEVADDMTE HMLELLLREL DVDPGDVVQV PGLLDLSCLW QVYGVDRPAL KDPTFVPATH PAFGQGETPK SIFSTLRDGD VLVHHPYDSF STSVQRFIEQ AAADPQVLAI KQTLYRTSGD SPIVNSLIDA AEAGKQVVAL VEIKARFDEQ ANIKWARALE DAGVHVVYGL IGLKTHCKTA LVVRREGSAI RRYCHIGTGN YNPKTARLYE DVGLLTSAPE IGADLTDLFN SLTGYSRKVS YRNLLVAPQG IRAGIVERID REIAAHRAGQ EARIRLKMNA LVDEQVIDAL YRASRAGVPI EVVVRGICAL RPGVPEYSGS ITVRSILGRF LEHSRIIHFN AIDEFWIGSA DMMHRNLDRR VEVLARVTDP KLTAQLDDMF TSALDSHTRC WELQPDGQWL ASPADGEEVR DHQVEMMKRH RSQT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaccggt acctcaaccg ggagctctcg tggctggatt tcaacgcgcg tgtgctcgca cttgccgccg acgaatcgct gcccatcctg gaacgcgcca aattcctggc aatcttcgcc tccaacctgg atgagtttta catggtgcgg gtcgccggtc tgaaacgccg tgacgagacg ggcttgtcgg tgcgttcggc cgacggccta tccccgcgcg aacagctgaa actgatcggt gagcgcacgc aggagatttc ggtccagcat gctcgggtat tcagtgagtc ggtgcgcccc gagctcgcag aaaacgggat ctttgtggtc acctgggctg acctgtcgga aaacgagcgc agttatcttt cctcgtattt caatgaccag gtcttccccg tgctcactcc gctggccgtc gaccccgcgc acccgttccc ctacatatcg ggcctgagcc tgaatctggc gatcaccgtc caagttcccg agacaggcgg ccagcatttc gcccgaatca aggtgcctga caatgtggat cgttttgtca gactgcgcag ccctgacggc actgagtcac cgcaggctgc ccataccgcg cgctttctgc cgatggagga actcatcgcc gcgcatctgt cggcgctgtt ccccggcctg gaaatcgtcg aacatcacgc gttccgcatc acccggaacg ctgatttcga ggtcgaggaa gatcgcgacg aggaccttct gcaggcactt gagcgggagc tggcgcgccg gcggttcggc tcgccggtgc gccttgaggt cgccgacgac atgaccgagc acatgctcga actgctactc cgcgagctgg acgtagatcc cggtgacgtc gtgcaggttc ccggcctgct ggatctgtcg tgcctgtggc aggtctacgg cgtggatcgg cccgccttga aggatccgac attcgtgccg gcgacccatc cggccttcgg gcagggtgaa acccccaaga gcatcttctc cacactgcgg gatggggatg tgctcgtgca tcacccgtat gactcgttct ccaccagcgt gcaacgattt atcgaacagg ccgccgcgga tcctcaggtg ctggccatca agcagacgct gtatcgcacc tccggcgact ctcctatcgt gaactcactc atcgacgccg ccgaggccgg taagcaggtt gtcgccctgg tggagatcaa ggcacgcttc gacgaacagg ccaatatcaa gtgggcgcgc gcgctggaag acgccggcgt acacgtggtc tacggcttga tcggcctcaa gacgcattgc aagacggccc tggtggtgcg ccgggaggga tcggccatcc gcaggtactg ccacatcggc accgggaact acaacccgaa gaccgcccgg ctttacgaag acgtcggcct gttgaccagc gcgccggaga ttggcgccga tctcaccgac ctgttcaact cgctgaccgg atactcgcga aaggtctcct atcgcaacct gttagtggcg ccccagggca tacgcgccgg gatcgtcgaa cgcatcgatc gtgagatcgc cgcacatcgg gccggccagg aggcccgcat ccggctgaag atgaatgcgc ttgtcgacga gcaggtgatc gatgcgctgt accgggcttc ccgagccggg gtgcccatcg aggtagtggt acgcgggatc tgcgcgctcc gtccaggggt tcctgagtac tcgggcagca ttaccgtccg atccattctc ggccggttcc tggaacattc gcgcatcatt cacttcaacg ccatcgacga attctggatc ggcagcgccg acatgatgca tcgtaatctg gaccgacggg tcgaggtcct cgcacgcgtc accgatccga agctcaccgc acagctcgac gacatgttca cctcggcgtt ggattcccac acccggtgct gggagctgca acccgacggc cagtggttgg cctcgcccgc ggacggcgag gaggtgcgcg accatcaggt ggagatgatg aaacggcacc gctcgcagac atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for MyabA.19295.a.B2.PW38243

PURIFICATION DATe:	4/10/2017
CONCENTRATION:	12mg/ml
OBSERVED MW:	82kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGACCGGT ACCTCAACCG GGAGCTCTCG TGGCTGGATT TCAACGCGCG TGTGCTCGCA CTTGCCGCCG ACGAATCGCT GCCCATCCTG GAACGCGCCA AATTCCTGGC AATCTTCGCC TCCAACCTGG ATGAGTTTTA CATGGTGCGG GTCGCCGGTC TGAAACGCCG TGACGAGACG GGCTTGTCGG TGCGTTCGGC CGACGGCCTA TCCCCGCGCG AACAGCTGAA ACTGATCGGT GAGCGCACGC AGGAGATTTC GGTCCAGCAT GCTCGGGTAT TCAGTGAGTC GGTGCGCCCC GAGCTCGCAG AAAACGGGAT CTTTGTGGTC ACCTGGGCTG ACCTGTCGGA AAACGAGCGC AGTTATCTTT CCTCGTATTT CAATGACCAG GTCTTCCCCG TGCTCACTCC GCTGGCCGTC GACCCCGCGC ACCCGTTCCC CTACATATCG GGCCTGAGCC TGAATCTGGC GATCACCGTC CAAGTTCCCG AGACAGGCGG CCAGCATTTC GCCCGAATCA AGGTGCCTGA CAATGTGGAT CGTTTTGTCA GACTGCGCAG CCCTGACGGC ACTGAGTCAC CGCAGGCTGC CCATACCGCG CGCTTTCTGC CGATGGAGGA ACTCATCGCC GCGCATCTGT CGGCGCTGTT CCCCGGCCTG GAAATCGTCG AACATCACGC GTTCCGCATC ACCCGGAACG CTGATTTCGA GGTCGAGGAA GATCGCGACG AGGACCTTCT GCAGGCACTT GAGCGGGAGC TGGCGCGCCG GCGGTTCGGC TCGCCGGTGC GCCTTGAGGT CGCCGACGAC ATGACCGAGC ACATGCTCGA ACTGCTACTC CGCGAGCTGG ACGTAGATCC CGGTGACGTC GTGCAGGTTC CCGGCCTGCT GGATCTGTCG TGCCTGTGGC AGGTCTACGG CGTGGATCGG CCCGCCTTGA AGGATCCGAC ATTCGTGCCG GCGACCCATC CGGCCTTCGG GCAGGGTGAA ACCCCCAAGA GCATCTTCTC CACACTGCGG GATGGGGATG TGCTCGTGCA TCACCCGTAT GACTCGTTCT CCACCAGCGT GCAACGATTT ATCGAACAGG CCGCCGCGGA TCCTCAGGTG CTGGCCATCA AGCAGACGCT GTATCGCACC TCCGGCGACT CTCCTATCGT GAACTCACTC ATCGACGCCG CCGAGGCCGG TAAGCAGGTT GTCGCCCTGG TGGAGATCAA GGCACGCTTC GACGAACAGG CCAATATCAA GTGGGCGCGC GCGCTGGAAG ACGCCGGCGT ACACGTGGTC TACGGCTTGA TCGGCCTCAA GACGCATTGC AAGACGGCCC TGGTGGTGCG CCGGGAGGGA TCGGCCATCC GCAGGTACTG CCACATCGGC ACCGGGAACT ACAACCCGAA GACCGCCCGG CTTTACGAAG ACGTCGGCCT GTTGACCAGC GCGCCGGAGA TTGGCGCCGA TCTCACCGAC CTGTTCAACT CGCTGACCGG ATACTCGCGA AAGGTCTCCT ATCGCAACCT GTTAGTGGCG CCCCAGGGCA TACGCGCCGG GATCGTCGAA CGCATCGATC GTGAGATCGC CGCACATCGG GCCGGCCAGG AGGCCCGCAT CCGGCTGAAG ATGAATGCGC TTGTCGACGA GCAGGTGATC GATGCGCTGT ACCGGGCTTC CCGAGCCGGG GTGCCCATCG AGGTAGTGGT ACGCGGGATC TGCGCGCTCC GTCCAGGGGT TCCTGAGTAC TCGGGCAGCA TTACCGTCCG ATCCATTCTC GGCCGGTTCC TGGAACATTC GCGCATCATT CACTTCAACG CCATCGACGA ATTCTGGATC GGCAGCGCCG ACATGATGCA TCGTAATCTG GACCGACGGG TCGAGGTCCT CGCACGCGTC ACCGATCCGA AGCTCACCGC ACAGCTCGAC GACATGTTCA CCTCGGCGTT GGATTCCCAC ACCCGGTGCT GGGAGCTGCA ACCCGACGGC CAGTGGTTGG CCTCGCCCGC GGACGGCGAG GAGGTGCGCG ACCATCAGGT GGAGATGATG AAACGGCACC GCTCGCAGAC ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT