MyabA.19286.a

Probable ubiquinol-cytochrome c reductase iron-sulfur subunit (Rieske iron-sulfur protein)

CENTER ID: MyabA.19286.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.19286.a.B2.GE40720 Rieske iron-sulfur domain 205 391
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2001
UniProt: B1MNZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVSDADKPE IPSDEQLAAM SRDELVKLGT NLDGVEIVYR EPRWPVEGTK AEKRAERLVA LWFALGGVFG LALLGVFLFW PWEYKPFGDP GNALYNLATP LYGLTLGLSV LSLGIGAVLY TKKFIPQEIS IQDRHDGPGS AEVDRKTIVA QLQDTLDTST LPRRKMIVRA LGFGVGAMGA GTAVAFIGGM IKNPWARVVP TANGKQPVLL TSGWTPRYHG ETIYLGRAVG SSSNVLLKIR PEDIDAGGME TVFPWRESDG DGTTVESHEK LSHIQMGIRN PVMLIRVRPT DMPKVVKRKG QENFNYGDLF AYTKICSHLG CPTSLFEQQT YRILCPCHQS QFDALHFAKP IFGPAARALA QLPLTVDKDG YLVANGDFVE PVGPAFWERK S
NT Sequence
gtgagcgtga gtgacgctga caagccagag atcccgagcg acgagcagct cgcggccatg agccgtgacg agctggtcaa gctcggcacc aacctcgacg gcgtcgagat tgtctaccgc gagccgcgct ggcccgtcga gggcaccaag gccgagaagc gtgccgagcg tctggtggcg ctgtggttcg cactcggtgg cgtgttcggc ctggccctgc tcggggtctt cctgttctgg ccctgggagt acaagccctt cggcgacccg ggcaacgcgc tctacaacct ggcgactccg ctctacggtc tgacgctggg cctgtcggtg ctgtcgctgg gtatcggtgc cgtgctgtac accaagaagt tcatcccgca ggagatctcc atccaggacc gtcacgacgg ccccggctcg gccgaggtgg atcgcaagac catcgtcgcc cagctgcagg acaccctgga cacctcgacc ctgccgcgcc gcaagatgat cgtccgggcc ctcggcttcg gcgtcggtgc catgggtgcc ggtaccgccg tcgccttcat tggcggcatg atcaagaacc cgtgggccag ggtggttccc accgccaacg gcaagcagcc ggtgctgctc accagtggct ggaccccgcg ctaccacggt gagaccatct acctgggccg cgccgtcggc agcagctcca acgtgctgct caagatccgc cccgaggaca tcgacgccgg cggcatggag accgtgttcc cgtggcgcga gtccgatggc gacggcacca ccgtcgaatc gcacgagaag ctgtcgcata tccagatggg catccgtaac cccgtgatgc tgatccgcgt gcgccccacc gatatgccca aggtggtcaa gcgcaagggg caagagaact tcaactacgg agacctcttc gcctacacca agatctgctc gcacctgggt tgccccacat cgctgttcga gcagcagacc taccgcatcc tgtgcccgtg ccaccagtcg cagttcgacg cactgcactt cgccaagccc atcttcgggc ctgccgctcg tgcgctggca cagctgcccc tcaccgtcga caaggacgga tacctcgtcg ccaacggcga cttcgttgaa cccgtcggac ccgcattctg ggagcgcaaa tcatga
Details for MyabA.19286.a.B2.GE40720
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 22kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK QPVLLTSGWT PRYHGETIYL GRAVGSSSNV LLKIRPEDID AGGMETVFPW RESDGDGTTV ESHEKLSHIQ MGIRNPVMLI RVRPTDMPKV VKRKGQENFN YGDLFAYTKI CSHLGCPTSL FEQQTYRILC PCHQSQFDAL HFAKPIFGPA ARALAQLPLT VDKDGYLVAN GDFVEPVGPA FWERKS
Validated NT Sequence
atggctcacc accaccacca ccatatgaag cagccggtgc tgctcaccag tggctggacc ccgcgctacc acggtgagac catctacctg ggccgcgccg tcggcagcag ctccaacgtg ctgctcaaga tccgccccga ggacatcgac gccggcggca tggagaccgt gttcccgtgg cgcgagtccg atggcgacgg caccaccgtc gaatcgcacg agaagctgtc gcatatccag atgggcatcc gtaaccccgt gatgctgatc cgcgtgcgcc ccaccgatat gcccaaggtg gtcaagcgca aggggcaaga gaacttcaac tacggagacc tcttcgccta caccaagatc tgctcgcacc tgggttgccc cacatcgctg ttcgagcagc agacctaccg catcctgtgc ccgtgccacc agtcgcagtt cgacgcactg cacttcgcca agcccatctt cgggcctgcc gctcgtgcgc tggcacagct gcccctcacc gtcgacaagg acggatacct cgtcgccaac ggcgacttcg ttgaacccgt cggacccgca ttctgggagc gcaaatcata agtgagtaag at
Expected Protein Sequence
MAHHHHHHKQ PVLLTSGWTP RYHGETIYLG RAVGSSSNVL LKIRPEDIDA GGMETVFPWR ESDGDGTTVE SHEKLSHIQM GIRNPVMLIR VRPTDMPKVV KRKGQENFNY GDLFAYTKIC SHLGCPTSLF EQQTYRILCP CHQSQFDALH FAKPIFGPAA RALAQLPLTV DKDGYLVANG DFVEPVGPAF WERKS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataagcagc cggtgctgct caccagtggc tggaccccgc gctaccacgg tgagaccatc tacctgggcc gcgccgtcgg cagcagctcc aacgtgctgc tcaagatccg ccccgaggac atcgacgccg gcggcatgga gaccgtgttc ccgtggcgcg agtccgatgg cgacggcacc accgtcgaat cgcacgagaa gctgtcgcat atccagatgg gcatccgtaa ccccgtgatg ctgatccgcg tgcgccccac cgatatgccc aaggtggtca agcgcaaggg gcaagagaac ttcaactacg gagacctctt cgcctacacc aagatctgct cgcacctggg ttgccccaca tcgctgttcg agcagcagac ctaccgcatc ctgtgcccgt gccaccagtc gcagttcgac gcactgcact tcgccaagcc catcttcggg cctgccgctc gtgcgctggc acagctgccc ctcaccgtcg acaaggacgg atacctcgtc gccaacggcg acttcgttga acccgtcgga cccgcattct gggagcgcaa atcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat