MyabA.19271.a

Probable ubiquinol-cytochrome c reductase cytochrome c subunit

CENTER ID: MyabA.19271.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.19271.a.B2.GE40719 cytochrome c-like domain 70 260
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2002
UniProt: B1MNZ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPDGPKTQPS PVKGAQQVSV EPVKDTAAKN RARRKFRRRV SAGLLLLIAL VVAGGLASTF TPTPQVAVAD EDKSALLRTG KQLYETSCIT CHGANLQGVP DRGPSLIGVG EAAVYFQVST GRMPAMRNEA QAQRKDPIFD EEQTDALGAY IQANGGGPQV IRDRDGAVAQ ESLRGNNIAR GGDLFRLNCA SCHNFTGRGG ALSSGKFAPE LDPATEQQIY TAMLTGPQNM PKFSDRQLTV DEKKDIIAYV RASSETPDPG GYGLGGFGPT SEGMAMWIIG IVAAIAAALW IGARA
NT Sequence
atgcccgacg gcccaaagac ccagcccagc ccggtgaagg gagcacaaca ggtgagcgtg gagcccgtaa aggacacagc cgccaagaat cgcgcacgcc gcaagttccg ccgtcgcgtc tcagcaggtt tgctgctgct gatcgcgctg gtggttgccg gcggcttggc ctctaccttc acgcccaccc cgcaggtcgc ggtggccgac gaggacaagt cggctctgct gcgcaccggt aagcagttgt acgagacctc gtgcatcacc tgccacggcg caaacctcca gggtgtgccg gaccgcgggc ccagcctgat cggcgtgggc gaggccgcgg tgtacttcca ggtctccacc ggacgtatgc cggccatgcg taatgaggct caggcccagc gcaaggaccc gatcttcgac gaggaacaga ccgacgccct gggcgcctac atccaggcca acggcggcgg cccccaggtg atccgcgacc gcgacggcgc agtggcccag gagtcgttgc gcggcaacaa catcgcccgt ggtggtgacc tgttccgtct caactgcgcg tcatgccaca acttcaccgg ccgcggtggc gcactctcgt cgggcaagtt cgcccccgag ctcgaccccg ccaccgagca gcagatttac accgcgatgc tgaccggacc gcagaacatg cccaagttct cggatcgcca gctcaccgtt gatgagaaga aggacatcat cgcctacgtc cgcgcctcgt ccgagacccc ggacccgggc ggttacggcc tgggcggctt cggaccgacg tccgagggca tggccatgtg gatcatcggc atcgtcgccg ccatcgccgc ggcactgtgg attggagcac gcgcgtga
Details for MyabA.19271.a.B2.GE40719
HARVESTED ON: 11/8/2016
SEQUENCED ON: 11/8/2016
EXPECTED MW: 22kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD EDKSALLRTG KQLYETSCIT CHGANLQGVP DRGPSLIGVG EAAVYFQVST GRMPAMRNEA QAQRKDPIFD EEQTDALGAY IQANGGGPQV IRDRDGAVAQ ESLRGNNIAR GGDLFRLNCA SCHNFTGRGG ALSSGKFAPE LDPATEQQIY TAMLTGPQNM PKFSDRQLTV DEKKDIIAYV RASSETPDPG
Validated NT Sequence
atggctcacc accaccacca ccatatggac gaggacaagt cggctctgct gcgcaccggt aagcagttgt acgagacctc gtgcatcacc tgccacggcg caaacctcca gggtgtgccg gaccgcgggc ccagcctgat cggcgtgggc gaggccgcgg tgtacttcca ggtctccacc ggacgtatgc cggccatgcg taatgaggct caggcccagc gcaaggaccc gatcttcgac gaggaacaga ccgacgccct gggcgcctac atccaggcca acggcggcgg cccccaggtg atccgcgacc gcgacggcgc agtggcccag gagtcgttgc gcggcaacaa catcgcccgt ggtggtgacc tgttccgtct caactgcgcg tcatgccaca acttcaccgg ccgcggtggc gcactctcgt cgggcaagtt cgcccccgag ctcgaccccg ccaccgagca gcagatttac accgcgatgc tgaccggacc gcagaacatg cccaagttct cggatcgcca gctcaccgtt gatgagaaga aggacatcat cgcctacgtc cgcgcctcgt ccgagacccc ggacccgggc taagtgagta agat
Expected Protein Sequence
MAHHHHHHDE DKSALLRTGK QLYETSCITC HGANLQGVPD RGPSLIGVGE AAVYFQVSTG RMPAMRNEAQ AQRKDPIFDE EQTDALGAYI QANGGGPQVI RDRDGAVAQE SLRGNNIARG GDLFRLNCAS CHNFTGRGGA LSSGKFAPEL DPATEQQIYT AMLTGPQNMP KFSDRQLTVD EKKDIIAYVR ASSETPDPG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacgagg acaagtcggc tctgctgcgc accggtaagc agttgtacga gacctcgtgc atcacctgcc acggcgcaaa cctccagggt gtgccggacc gcgggcccag cctgatcggc gtgggcgagg ccgcggtgta cttccaggtc tccaccggac gtatgccggc catgcgtaat gaggctcagg cccagcgcaa ggacccgatc ttcgacgagg aacagaccga cgccctgggc gcctacatcc aggccaacgg cggcggcccc caggtgatcc gcgaccgcga cggcgcagtg gcccaggagt cgttgcgcgg caacaacatc gcccgtggtg gtgacctgtt ccgtctcaac tgcgcgtcat gccacaactt caccggccgc ggtggcgcac tctcgtcggg caagttcgcc cccgagctcg accccgccac cgagcagcag atttacaccg cgatgctgac cggaccgcag aacatgccca agttctcgga tcgccagctc accgttgatg agaagaagga catcatcgcc tacgtccgcg cctcgtccga gaccccggac ccgggctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t