SSGCID: Target Page

MyabA.17943.a

Leucine--tRNA ligase (EC 6.1.1.4) (Leucyl-tRNA synthetase) (LeuRS)

CENTER ID:	MyabA.17943.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.17943.a.B1.GE42452	full length		1	951

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.17943.a.B1.PW38504	full length		1	951

External Resources

RESOURCE	REFERENCE ID
UniProt:	B1MMK6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTETQHDATD TAELPRHRYT AQLAGQIERR WQQTWADRGT FHVPNPVGSL APTDGTPIPA DKMFVQDMFP YPSGDGLHVG HPLGYIATDV YARFHRMRGA NVLHALGFDA FGLPAEQYAV QTGTHPRVRT EANIVNYKRQ LGRLGLGHDS RRSFATTDVD FYKWTQWIFL QIYNAWYDEQ ARRARPIQEL IAEFDSGTRA PSDGTVWAEL SVGARADVID SYRLVYQSDS VVNWCPGLGT VLANEEVTAD GRSERGNFPV FRKRLRQWMM RITAYSDRLI DDLDVLDWPD KVKTMQRNWI GRSQGASVLF GAPGAGDIEV FTTRPDTLFG ATYMVLAPEH PLVDQLAADV WPQDTDPRWT GGQDSPRAAI EQYRRSIAAK SDLERQENKE KTGVFTGAYA TNPVSGKPVP VFIADYVLLG YGTGAIMAVP GHDQRDWDFA NTFGLPVQEV ISGGDVTKAA YTGDGVLVNS DYLDGLDIEA AKVEVTRRLV KDGRGESRIE YKLRDWLFAR QRYWGEPFPI VYDEDGRPRA LGENVLPVEL PEVEDYAPVS FDPDDASSEP SPPLSKAADW VNVELDLGDG LKHYTRDTNV MPQWAGSSWY ELRYADPDNA EAFCDKENEA YWLGPRPAEH GPNDPGGVDL YVGGMEHAVL HLLYSRFWHK VLYDLGHVSS REPYRRLLNQ GYIQAHAYTD ARGMYVPAAE VTEENGKFFY QGAEVQQEFG KIGKSLKNSI SPDDICDNYG ADTLRVYEMS MGPLELSRPW ATKDVVGAHR FLQRAWRVVV DEETGKIRVT TDDLTSEDTL RALHKTIAGV TEDYAALRNN TAAAKLIEYT NHLTKDYPEG APRAAVEPLV LMLAPLAPHL AEELWSLLGR DDSLAHGPFP ESDDRWLVAD TVEYPIQVNG KVRGRITVAA DAPKGDIEAA ALTEEKVLEF LAGATPKKVI VVPGRMVNLV V

NT Sequence

gtgaccgaaa cccagcacga cgcgaccgat accgctgagt tgcccaggca tcgctatacc gcgcagctcg ccgggcagat cgagcgccgc tggcaacaga cctgggccga ccggggtacg ttccatgttc cgaacccggt gggatcgctt gcgccgacgg acggcacgcc gattcccgcc gacaagatgt tcgtgcagga catgttcccc tacccctcgg gcgacggcct gcacgtcgga catccgctcg gatacatcgc gaccgacgtc tatgcgcgct ttcaccggat gcgtggtgcc aacgtgctgc acgccctggg gttcgacgcg ttcgggttgc ccgccgagca gtacgcggtg cagaccggca cccatccacg ggtccggacc gaggcgaaca tcgtcaacta caagcggcag ctgggtcggc tggggctggg tcacgactcg cgccgcagct tcgccaccac cgatgtcgac ttctacaagt ggacgcagtg gatcttccta cagatctaca acgcctggta cgacgagcag gcccgccgtg ccaggcccat ccaggagctg atcgccgagt tcgactccgg aacgcgcgca ccctccgacg gcaccgtgtg ggcggagctg agtgttggtg cgcgggcgga tgttatcgac tcatatcgcc tggtgtacca atcggattcg gtggtcaact ggtgtccggg attgggcacc gtgctggcca acgaagaggt caccgcggac gggcgcagcg agcgcggcaa cttcccggtg ttccgtaagc gcctgcggca gtggatgatg cggattaccg cctactccga caggttgatc gatgacctag acgtgctgga ctggccggac aaggtcaaaa ccatgcagcg caactggatc ggccgctcgc agggcgcatc ggtgttgttc ggagcgcctg gcgcggggga catcgaggtg ttcaccacca ggcccgacac gctcttcggc gccacctaca tggtgctggc cccggagcat ccgctggtgg accagttggc ggcggatgtc tggccgcagg acaccgatcc gcgttggacc ggcgggcagg attcgccgcg ggcggcgatc gaacagtacc gccgctccat cgccgccaag agcgatctgg aacgtcagga gaacaaggaa aagaccggcg tcttcaccgg cgcatacgcc accaacccgg tgagcgggaa gccggtgccg gttttcatcg cggactacgt gctgctgggg tatggcaccg gtgccatcat ggcggttccc gggcacgacc agcgtgactg ggacttcgcg aacaccttcg gtttgccggt gcaggaggtg atttccgggg gcgacgtcac caaggccgca tacaccgggg acggtgtgct ggtgaattcc gactacctgg acgggctcga catcgaggcc gccaaggtgg aggtgacacg gcgtttggtc aaagacggac gcggcgaatc gcgcatcgaa tacaaactgc gcgattggct ctttgcgcgc cagcgctatt ggggtgagcc cttcccgatc gtctacgacg aggacgggcg gccacgtgcg ctgggtgaga acgtattacc cgtcgaattg cccgaggtcg aggattacgc accggtgtcc ttcgatcccg acgacgcgtc atcggagccg tcaccaccgc tgtccaaggc cgccgactgg gtgaatgtcg agctggacct cggcgatggg ctcaagcact acacgcggga caccaacgtg atgccgcagt gggcgggcag ctcctggtat gagctgcgct acgccgaccc cgacaacgca gaggccttct gcgacaagga gaacgaggca tactggctgg gcccgcgccc ggcggaacat ggcccgaatg acccgggcgg tgtcgatctg tatgtcggcg gcatggaaca cgccgtgctg cacctgctgt attcgcggtt ctggcacaag gtgctgtacg acctggggca cgtcagttcc cgcgagccgt accggcggct gctcaatcag gggtacatcc aggcccacgc ctacacggat gcgcgcggca tgtatgtgcc ggcggccgag gtcaccgagg aaaacggaaa gttcttctac cagggcgcag aggtgcaaca agagttcggg aagatcggaa agagcctcaa gaactcgatc tcgccggatg acatctgcga caactatggt gccgatacgc tgcgtgtgta cgagatgtcg atgggcccgc tggagctgtc gcgtccctgg gccacgaagg atgtcgtggg tgcacaccgc tttttgcaac gcgcgtggcg cgtggtggtc gatgaggaga ccggcaagat ccgtgtcacc acggacgacc tgaccagcga ggacaccctg cgggcgttac acaagacgat cgccggagtg acggaagact atgcggccct acgtaataac accgccgcgg ccaagctgat cgaatacacc aatcacctga ccaaggacta cccggagggt gcgccgcgcg cggcggtgga gccgctggtg ctcatgctgg caccgttggc tccgcatctg gccgaggagc tgtggagcct gctgggccgc gacgactcgc tggctcacgg tcccttcccg gaatccgacg accgctggct ggtggccgac acggtcgagt acccgattca ggtgaacggc aaggtgcgtg gccgcatcac cgtcgccgcc gatgcgccca agggcgacat cgaggcagcg gccctcaccg aggagaaagt gctggagttc ctggccggcg ccaccccgaa gaaggtcatt gtggtgcccg gccgcatggt gaacctggtc gtctag

Details for MyabA.17943.a.B1.GE42452

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	107kDa
OBSERVED MW:	107kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT ETQHDATDTA ELPRHRYTAQ LAGQIERRWQ QTWADRGTFH VPNPVGSLAP TDGTPIPADK MFVQDMFPYP SGDGLHVGHP LGYIATDVYA RFHRMRGANV LHALGFDAFG LPAEQYAVQT GTHPRVRTEA NIVNYKRQLG RLGLGHDSRR SFATTDVDFY KWTQWIFLQI YNAWYDEQAR RARPIQELIA EFDSGTRAPS DGTVWAELSV GARADVIDSY RLVYQSDSVV NWCPGLGTVL ANEEVTADGR SERGNFPVFR KRLRQWMMRI TAYSDRLIDD LDVLDWPDKV KTMQRNWIGR SQGASVLFGA PGAGDIEVFT TRPDTLFGAT YMVLAPEHPL VDQLAADVWP QDTDPRWTGG QDSPRAAIEQ YRRSIAAKSD LERQENKEKT GVFTGAYATN PVSGKPVPVF IADYVLLGYG TGAIMAVPGH DQRDWDFANT FGLPVQEVIS GGDVTKAAYT GDGVLVNSDY LDGLDIEAAK VEVTRRLVKD GRGESRIEYK LRDWLFARQR YWGEPFPIVY DEDGRPRALG ENVLPVELPE VEDYAPVSFD PDDASSEPSP PLSKAADWVN VELDLGDGLK HYTRDTNVMP QWAGSSWYEL RYADPDNAEA FCDKENEAYW LGPRPAEHGP NDPGGVDLYV GGMEHAVLHL LYSRFWHKVL YDLGHVSSRE PYRRLLNQGY IQAHAYTDAR GMYVPAAEVT EENGKFFYQG AEVQQEFGKI GKSLKNSISP DDICDNYGAD TLRVYEMSMG PLELSRPWAT KDVVGAHRFL QRAWRVVVDE ETGKIRVTTD DLTSEDTLRA LHKTIAGVTE DYAALRNNTA AAKLIEYTNH LTKDYPEGAP RAAVEPLVLM LAPLAPHLAE ELWSLLGRDD SLAHGPFPES DDRWLVADTV EYPIQVNGKV RGRITVAADA PKGDIEAAAL TEEKVLEFLA GATPKKVIVV PGRMVNLVV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaccg aaacccagca cgacgcgacc gataccgctg agttgcccag gcatcgctat accgcgcagc tcgccgggca gatcgagcgc cgctggcaac agacctgggc cgaccggggt acgttccatg ttccgaaccc ggtgggatcg cttgcgccga cggacggcac gccgattccc gccgacaaga tgttcgtgca ggacatgttc ccctacccct cgggcgacgg cctgcacgtc ggacatccgc tcggatacat cgcgaccgac gtctatgcgc gctttcaccg gatgcgtggt gccaacgtgc tgcacgccct ggggttcgac gcgttcgggt tgcccgccga gcagtacgcg gtgcagaccg gcacccatcc acgggtccgg accgaggcga acatcgtcaa ctacaagcgg cagctgggtc ggctggggct gggtcacgac tcgcgccgca gcttcgccac caccgatgtc gacttctaca agtggacgca gtggatcttc ctacagatct acaacgcctg gtacgacgag caggcccgcc gtgccaggcc catccaggag ctgatcgccg agttcgactc cggaacgcgc gcaccctccg acggcaccgt gtgggcggag ctgagtgttg gtgcgcgggc ggatgttatc gactcatatc gcctggtgta ccaatcggat tcggtggtca actggtgtcc gggattgggc accgtgctgg ccaacgaaga ggtcaccgcg gacgggcgca gcgagcgcgg caacttcccg gtgttccgta agcgcctgcg gcagtggatg atgcggatta ccgcctactc cgacaggttg atcgatgacc tagacgtgct ggactggccg gacaaggtca aaaccatgca gcgcaactgg atcggccgct cgcagggcgc atcggtgttg ttcggagcgc ctggcgcggg ggacatcgag gtgttcacca ccaggcccga cacgctcttc ggcgccacct acatggtgct ggccccggag catccgctgg tggaccagtt ggcggcggat gtctggccgc aggacaccga tccgcgttgg accggcgggc aggattcgcc gcgggcggcg atcgaacagt accgccgctc catcgccgcc aagagcgatc tggaacgtca ggagaacaag gaaaagaccg gcgtcttcac cggcgcatac gccaccaacc cggtgagcgg gaagccggtg ccggttttca tcgcggacta cgtgctgctg gggtatggca ccggtgccat catggcggtt cccgggcacg accagcgtga ctgggacttc gcgaacacct tcggtttgcc ggtgcaggag gtgatttccg ggggcgacgt caccaaggcc gcatacaccg gggacggtgt gctggtgaat tccgactacc tggacgggct cgacatcgag gccgccaagg tggaggtgac acggcgtttg gtcaaagacg gacgcggcga atcgcgcatc gaatacaaac tgcgcgattg gctctttgcg cgccagcgct attggggtga gcccttcccg atcgtctacg acgaggacgg gcggccacgt gcgctgggtg agaacgtatt acccgtcgaa ttgcccgagg tcgaggatta cgcaccggtg tccttcgatc ccgacgacgc gtcatcggag ccgtcaccac cgctgtccaa ggccgccgac tgggtgaatg tcgagctgga cctcggcgat gggctcaagc actacacgcg ggacaccaac gtgatgccgc agtgggcggg cagctcctgg tatgagctgc gctacgccga ccccgacaac gcagaggcct tctgcgacaa ggagaacgag gcatactggc tgggcccgcg cccggcggaa catggcccga atgacccggg cggtgtcgat ctgtatgtcg gcggcatgga acacgccgtg ctgcacctgc tgtattcgcg gttctggcac aaggtgctgt acgacctggg gcacgtcagt tcccgcgagc cgtaccggcg gctgctcaat caggggtaca tccaggccca cgcctacacg gatgcgcgcg gcatgtatgt gccggcggcc gaggtcaccg aggaaaacgg aaagttcttc taccagggcg cagaggtgca acaagagttc gggaagatcg gaaagagcct caagaactcg atctcgccgg atgacatctg cgacaactat ggtgccgata cgctgcgtgt gtacgagatg tcgatgggcc cgctggagct gtcgcgtccc tgggccacga aggatgtcgt gggtgcacac cgctttttgc aacgcgcgtg gcgcgtggtg gtcgatgagg agaccggcaa gatccgtgtc accacggacg acctgaccag cgaggacacc ctgcgggcgt tacacaagac gatcgccgga gtgacggaag actatgcggc cctacgtaat aacaccgccg cggccaagct gatcgaatac accaatcacc tgaccaagga ctacccggag ggtgcgccgc gcgcggcggt ggagccgctg gtgctcatgc tggcaccgtt ggctccgcat ctggccgagg agctgtggag cctgctgggc cgcgacgact cgctggctca cggtcccttc ccggaatccg acgaccgctg gctggtggcc gacacggtcg agtacccgat tcaggtgaac ggcaaggtgc gtggccgcat caccgtcgcc gccgatgcgc ccaagggcga catcgaggca gcggccctca ccgaggagaa agtgctggag ttcctggccg gcgccacccc gaagaaggtc attgtggtgc ccggccgcat ggtgaacctg gtcgtctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for MyabA.17943.a.B1.PW38504

PURIFICATION DATe:	7/9/2018
CONCENTRATION:	27.85mg/ml
OBSERVED MW:	107kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGACCG AAACCCAGCA CGACGCGACC GATACCGCTG AGTTGCCCAG GCATCGCTAT ACCGCGCAGC TCGCCGGGCA GATCGAGCGC CGCTGGCAAC AGACCTGGGC CGACCGGGGT ACGTTCCATG TTCCGAACCC GGTGGGATCG CTTGCGCCGA CGGACGGCAC GCCGATTCCC GCCGACAAGA TGTTCGTGCA GGACATGTTC CCCTACCCCT CGGGCGACGG CCTGCACGTC GGACATCCGC TCGGATACAT CGCGACCGAC GTCTATGCGC GCTTTCACCG GATGCGTGGT GCCAACGTGC TGCACGCCCT GGGGTTCGAC GCGTTCGGGT TGCCCGCCGA GCAGTACGCG GTGCAGACCG GCACCCATCC ACGGGTCCGG ACCGAGGCGA ACATCGTCAA CTACAAGCGG CAGCTGGGTC GGCTGGGGCT GGGTCACGAC TCGCGCCGCA GCTTCGCCAC CACCGATGTC GACTTCTACA AGTGGACGCA GTGGATCTTC CTACAGATCT ACAACGCCTG GTACGACGAG CAGGCCCGCC GTGCCAGGCC CATCCAGGAG CTGATCGCCG AGTTCGACTC CGGAACGCGC GCACCCTCCG ACGGCACCGT GTGGGCGGAG CTGAGTGTTG GTGCGCGGGC GGATGTTATC GACTCATATC GCCTGGTGTA CCAATCGGAT TCGGTGGTCA ACTGGTGTCC GGGATTGGGC ACCGTGCTGG CCAACGAAGA GGTCACCGCG GACGGGCGCA GCGAGCGCGG CAACTTCCCG GTGTTCCGTA AGCGCCTGCG GCAGTGGATG ATGCGGATTA CCGCCTACTC CGACAGGTTG ATCGATGACC TAGACGTGCT GGACTGGCCG GACAAGGTCA AAACCATGCA GCGCAACTGG ATCGGCCGCT CGCAGGGCGC ATCGGTGTTG TTCGGAGCGC CTGGCGCGGG GGACATCGAG GTGTTCACCA CCAGGCCCGA CACGCTCTTC GGCGCCACCT ACATGGTGCT GGCCCCGGAG CATCCGCTGG TGGACCAGTT GGCGGCGGAT GTCTGGCCGC AGGACACCGA TCCGCGTTGG ACCGGCGGGC AGGATTCGCC GCGGGCGGCG ATCGAACAGT ACCGCCGCTC CATCGCCGCC AAGAGCGATC TGGAACGTCA GGAGAACAAG GAAAAGACCG GCGTCTTCAC CGGCGCATAC GCCACCAACC CGGTGAGCGG GAAGCCGGTG CCGGTTTTCA TCGCGGACTA CGTGCTGCTG GGGTATGGCA CCGGTGCCAT CATGGCGGTT CCCGGGCACG ACCAGCGTGA CTGGGACTTC GCGAACACCT TCGGTTTGCC GGTGCAGGAG GTGATTTCCG GGGGCGACGT CACCAAGGCC GCATACACCG GGGACGGTGT GCTGGTGAAT TCCGACTACC TGGACGGGCT CGACATCGAG GCCGCCAAGG TGGAGGTGAC ACGGCGTTTG GTCAAAGACG GACGCGGCGA ATCGCGCATC GAATACAAAC TGCGCGATTG GCTCTTTGCG CGCCAGCGCT ATTGGGGTGA GCCCTTCCCG ATCGTCTACG ACGAGGACGG GCGGCCACGT GCGCTGGGTG AGAACGTATT ACCCGTCGAA TTGCCCGAGG TCGAGGATTA CGCACCGGTG TCCTTCGATC CCGACGACGC GTCATCGGAG CCGTCACCAC CGCTGTCCAA GGCCGCCGAC TGGGTGAATG TCGAGCTGGA CCTCGGCGAT GGGCTCAAGC ACTACACGCG GGACACCAAC GTGATGCCGC AGTGGGCGGG CAGCTCCTGG TATGAGCTGC GCTACGCCGA CCCCGACAAC GCAGAGGCCT TCTGCGACAA GGAGAACGAG GCATACTGGC TGGGCCCGCG CCCGGCGGAA CATGGCCCGA ATGACCCGGG CGGTGTCGAT CTGTATGTCG GCGGCATGGA ACACGCCGTG CTGCACCTGC TGTATTCGCG GTTCTGGCAC AAGGTGCTGT ACGACCTGGG GCACGTCAGT TCCCGCGAGC CGTACCGGCG GCTGCTCAAT CAGGGGTACA TCCAGGCCCA CGCCTACACG GATGCGCGCG GCATGTATGT GCCGGCGGCC GAGGTCACCG AGGAAAACGG AAAGTTCTTC TACCAGGGCG CAGAGGTGCA ACAAGAGTTC GGGAAGATCG GAAAGAGCCT CAAGAACTCG ATCTCGCCGG ATGACATCTG CGACAACTAT GGTGCCGATA CGCTGCGTGT GTACGAGATG TCGATGGGCC CGCTGGAGCT GTCGCGTCCC TGGGCCACGA AGGATGTCGT GGGTGCACAC CGCTTTTTGC AACGCGCGTG GCGCGTGGTG GTCGATGAGG AGACCGGCAA GATCCGTGTC ACCACGGACG ACCTGACCAG CGAGGACACC CTGCGGGCGT TACACAAGAC GATCGCCGGA GTGACGGAAG ACTATGCGGC CCTACGTAAT AACACCGCCG CGGCCAAGCT GATCGAATAC ACCAATCACC TGACCAAGGA CTACCCGGAG GGTGCGCCGC GCGCGGCGGT GGAGCCGCTG GTGCTCATGC TGGCACCGTT GGCTCCGCAT CTGGCCGAGG AGCTGTGGAG CCTGCTGGGC CGCGACGACT CGCTGGCTCA CGGTCCCTTC CCGGAATCCG ACGACCGCTG GCTGGTGGCC GACACGGTCG AGTACCCGAT TCAGGTGAAC GGCAAGGTGC GTGGCCGCAT CACCGTCGCC GCCGATGCGC CCAAGGGCGA CATCGAGGCA GCGGCCCTCA CCGAGGAGAA AGTGCTGGAG TTCCTGGCCG GCGCCACCCC GAAGAAGGTC ATTGTGGTGC CCGGCCGCAT GGTGAACCTG GTCGTCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T