MyabA.17908.a

Demethylmenaquinone methyltransferase (EC 2.1.1.163)

CENTER ID: MyabA.17908.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.17908.a.B1.GE43879 full length 1 227
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: B1MHC3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRATLEKDP HEVASMFDAV ARRYDITNTV LSFGRDRFWR RATREALRLK PGDKVLDIAA GTAVSTAELT QSGAWCVAAD FSVGMLKAGA RRDVPKVAGD ATRLPFADHS FDAVTISFGL RNVVDHVEGL REMARVTKPG GRLAVCEFST PVNRPFRTLY MEYLMKTLPE VARAVSSNPD AYVYLAESIR AWPDQEALAH QIASAGWSNV RWRNLTGGIV ALHIATL
NT Sequence
atgaaccgcg ccaccctgga aaaggacccg cacgaggtcg cgtccatgtt cgacgcggtg gcccgtaggt atgacatcac caataccgtg ctctccttcg gccgggaccg cttttggcgc cgcgccaccc gcgaggccct caggctcaag cccggcgaca aggtgctcga catcgccgcg ggaaccgcgg tctcgaccgc cgaactcacc cagtccggtg cgtggtgcgt ggccgcggac ttctcggtcg gcatgctcaa ggccggtgcc cgtcgcgacg tgccgaaggt ggccggggac gccacgcggc tgccgttcgc ggatcactcg ttcgacgcgg tgaccatcag ctttgggctg cgtaatgtcg tcgatcacgt cgaagggctg cgggaaatgg ctcgtgtcac caaacccggt ggccgccttg cggtatgcga gttctcgacg ccggtcaacc ggccgtttcg cacgctgtac atggaatatc tgatgaagac gctgcccgag gtggcgcgcg ccgtcagcag caatccggat gcctacgtgt acctggccga gtccatccgg gcgtggcccg atcaggaggc gctggcccac cagatcgcct cggcagggtg gtcaaatgtg cggtggcgca atctcaccgg tggcatcgtc gccctccaca tcgccacttt gtaa
Details for MyabA.17908.a.B1.GE43879
HARVESTED ON: 11/2/2020
SEQUENCED ON: 12/2/2020
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 90
Validated AA Sequence
MFDAVARRYD ITNTVLSFGR DRFWRRATRE ALRLKPGDKV LDIAAGTAVS TAELTQSGAW CVAADFSVGM LKAGARRDVP KVAGDATRLP FADHSFDAVT ISFGLRNVVD HVEGLREMAR VTKPGGRLAV CEFSTPVNRP FRTLYMEYLM KTLPEVARAV SSNPDAYVYL AESIRAWPDQ EALAHQIASA GWSNVRWRNL TGGIVALHIA TL
Validated NT Sequence
tnctttcggg ctttgttagc agccggatcc tatcttactc acttacaaag tggcgatgtg gagggcgacg atgccaccgg tgagattgcg ccaccgcaca tttgaccacc ctgccgaggc gatctggtgg gccagcgcct cctgatcggg ccacgcccgg atggactcgg ccaggtacac gtaggcatcc ggattgctgc tgacggcgcg cgccacctcg ggcagcgtct tcatcagata ttccatgtac agcgtgcgaa acggccggtt gaccggcgtc gagaactcgc ataccgcaag gcggccaccg ggtttggtga cacgagccat ttcccgcagc ccttcgacgt gatcgacgac attacgcagc ccaaagctga tggtcaccgc gtcgaacgag tgatccgcga acggcagccg cgtggcgtcc ccggccacct tcggcacgtc gcgacgggca ccggccttga gcatgccgac cgagaagtcc gcggccacgc accacgcacc ggactgggtg agttcggcgg tcgagaccgc ggttcccgcg gcgatgtcga gcaccttgtc gccgggcttg agcctgaggg cctcgcgggt ggcgcggcgc caaaagcggt cccggccgaa ggagagcacg gtattggtga tgtcatacct acgggccacc gcgtcgaaca tggacgcgac ctcgtgcggg tccttttcca ggtggcgcgg ttcatatggt ggtggnnngg ggnngagcca tggtatatct ccttcttaaa gttaaacaaa attatttcta gagggaaacc gttgtggtct ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcggct cgcncttcgg gctcatgagc gcttgtttcg ncgtgggggt ggnaggcccn nnnngggnnn tgntnnnncn tctcntgcat gnnnnntnnn ngngcgngct nannngnntc annantacnn gnntgntnnt annncnnnnn catnnnnnna nnnnncgann nnnngnccnt g
Expected Protein Sequence
MAHHHHHHMN RATLEKDPHE VASMFDAVAR RYDITNTVLS FGRDRFWRRA TREALRLKPG DKVLDIAAGT AVSTAELTQS GAWCVAADFS VGMLKAGARR DVPKVAGDAT RLPFADHSFD AVTISFGLRN VVDHVEGLRE MARVTKPGGR LAVCEFSTPV NRPFRTLYME YLMKTLPEVA RAVSSNPDAY VYLAESIRAW PDQEALAHQI ASAGWSNVRW RNLTGGIVAL HIATL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc gcgccaccct ggaaaaggac ccgcacgagg tcgcgtccat gttcgacgcg gtggcccgta ggtatgacat caccaatacc gtgctctcct tcggccggga ccgcttttgg cgccgcgcca cccgcgaggc cctcaggctc aagcccggcg acaaggtgct cgacatcgcc gcgggaaccg cggtctcgac cgccgaactc acccagtccg gtgcgtggtg cgtggccgcg gacttctcgg tcggcatgct caaggccggt gcccgtcgcg acgtgccgaa ggtggccggg gacgccacgc ggctgccgtt cgcggatcac tcgttcgacg cggtgaccat cagctttggg ctgcgtaatg tcgtcgatca cgtcgaaggg ctgcgggaaa tggctcgtgt caccaaaccc ggtggccgcc ttgcggtatg cgagttctcg acgccggtca accggccgtt tcgcacgctg tacatggaat atctgatgaa gacgctgccc gaggtggcgc gcgccgtcag cagcaatccg gatgcctacg tgtacctggc cgagtccatc cgggcgtggc ccgatcagga ggcgctggcc caccagatcg cctcggcagg gtggtcaaat gtgcggtggc gcaatctcac cggtggcatc gtcgccctcc acatcgccac tttgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat