SSGCID: Target Page

MyabA.17897.a

ATP-dependent zinc metalloprotease FtsH (Rv3610c ortholog)

CENTER ID:	MyabA.17897.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.17897.a.B2.GE37326	soluble domain		133	750

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.573
RefSeq:	YP_001701286.1
UniProt:	B1MGU8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNRTNVFRTL GVIVVVLLLG WSFFYFSDDT RGYKPVDTTV AIKQIDTDNV KSARIDDREQ QLRLDLKAAN GDTEGKDKVI TKYPTGYGVP LLEKLNGKGA AVNTTVNQGS ILGSLLLYLL PVILLVVLFF AFSRMQSGGR GMGFGFGKSR AKQLSKDMPK TTFADVAGVD EAVEELYEIK DFLQNPSRYQ ALGAKIPRGV LLYGPPGTGK TLLARAVAGE AGVPFFTISG SDFVEMFVGV GASRVRDLFE QAKQNSPCII FVDEIDAVGR QRGAGLGGGH DEREQTLNQL LVEMDGFGDR QGVILIAATN RPDILDPALL RPGRFDRQIP VSSPDLAGRK AVLKVHSAGK PFGPDVDFDG LAKRTVGMSG ADLANVINEA ALLTARENGT VITAAALEES VDRVVGGPRR KGRIISEHEK KITAYHEAGH TLAAWAMPDI ERVYKVTILA RGRTGGHAIA VPEDDKGLAT RSEMIAQLVF AMGGRAAEEL VFREPTTGAV SDIEQATKKA RAMVTEFGMS AKLGAVRYGT EHGDPFLGRT MGTQADYSHE VAREIDEEVR NLIEAAHTEA WAILTEYRDV LDTLAGALLE KETVVRKELE EIFSGVQRRP RLTMFDDFGG RIPSDKPPIK TPGELAIERG EPWPPPVPEP AFKKAIAEQA AAAAPANGVP PAPAGPHGHL PNGQGGQPVP PGVNYGAPAG WHAPGWPPPG QAPHPQPGYQ PYPPQPYPYP VPTPHQQPGP APDEGSESKG

NT Sequence

atgaaccgga caaacgtgtt tcgcaccctg ggcgtcatcg tggtggtgct gctgctgggg tggtcgttct tctacttcag cgacgacacc cgcggttaca agcccgtgga caccacggtc gcgatcaagc agatcgacac cgacaacgtc aaaagcgccc gcattgacga ccgtgagcag cagctgcggc tggacctgaa ggccgccaac ggtgataccg aaggtaagga caaggtcatc accaagtacc ccaccgggta cggggtgcca ttactggaga agctcaacgg caagggcgcg gccgtcaaca cgacggtcaa ccagggcagc atcctgggat cgctgctgct gtacctgctg ccggtgatcc tgctggtggt gctgttcttc gcgttcagcc ggatgcagtc cgggggccgg ggcatgggat tcggtttcgg taaatcgcgt gccaagcagc tgtccaagga catgcccaag accaccttcg ccgacgtggc aggtgtcgac gaggcggtcg aggagctcta cgagatcaag gacttcctgc agaacccgtc gcggtatcag gcgctcggtg ccaagatccc gcgtggcgtg ctgctctatg gcccgcccgg caccggtaag acgctgttgg cccgtgcggt cgccggtgag gcaggagtgc cgttctttac catctccggc tccgacttcg tggaaatgtt tgtgggcgtt ggcgcttcgc gcgtgcgtga cctgtttgag caggccaagc agaacagtcc ctgcatcatt ttcgtcgacg agatcgacgc agtcggacgg cagcgtggcg ccggcctggg cggtgggcac gacgagcgtg aacagaccct gaaccagctg ttggtggaga tggatggctt cggtgaccgg cagggtgtca tcctgatcgc cgcgaccaac cgccccgaca tcctggatcc ggcactgttg cgtcccggtc gtttcgaccg gcagatcccg gtgtccagcc ccgacctggc gggccgcaag gccgtgctca aagtccattc ggcgggcaag cccttcggcc ccgatgtcga cttcgatggc ctggccaagc gcaccgtcgg catgtccggt gccgaccttg ccaacgtgat caacgaggcg gcgttgctca cggcccgtga gaacggcacc gtgatcacgg ctgccgcgct ggaggagtcg gtcgacaggg tcgttggtgg tccccgtcgc aaggggcgca tcatcagcga gcacgaaaag aagatcaccg cgtatcacga ggccggccac acgctggctg cctgggccat gcccgatatc gagcgggtgt acaaggtgac catcttggcg cgtgggcgta ccggcggaca cgccatagcg gttcccgagg atgacaaggg cctggccacc aggtcggaaa tgatcgcgca gctggtcttc gcgatgggcg gccgggcggc cgaagaactc gtgttccggg agccgaccac cggtgcggtg tccgatatcg aacaggccac caagaaggcc cgtgcgatgg tcaccgagtt cgggatgagc gccaagctcg gagcggtgcg ctacggcact gaacacggtg atccgttcct gggccgcacg atgggcaccc aggctgatta ctcccatgag gtcgcccgcg aaatcgatga ggaggtgcgc aatctcatcg aggcggcgca caccgaggca tgggcgatcc tcaccgagta ccgggatgtg ctggacaccc tggccggcgc actgctggag aaggaaacgg tggtccgtaa ggaactcgag gaaatcttct ccggcgtcca gcgccgtccg cggttgacca tgttcgacga ctttggcggt cgtatcccct ccgacaagcc gcccatcaag acgcccggcg agcttgctat cgaacgtggc gaaccctggc ccccaccggt cccggagccc gcgttcaaga aggccatcgc cgagcaggcg gctgcggcgg cgcccgccaa tggggtgcca ccggcgcctg cgggtccgca tggacatctc cccaacggcc aaggtggaca gcccgttccg cctggagtca actatggggc tcccgccggc tggcacgcac ccggctggcc tccccccggg caggctccgc acccgcagcc gggctatcag ccatatccgc cgcagcccta cccgtacccg gtgccgactc cgcatcagca gccgggcccg gctccggacg agggcagcga gagcaagggc tga

Details for MyabA.17897.a.B2.GE37326

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	67kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	92
PERCENT COVERAGE:	96

Validated AA Sequence

MQSGGRGMGF GFGKSRAKQL SKDMPKTTFA DVAGVDEAVE ELYEIKDFLQ NPSRYQALGA KIPRGVLLYG PPGTGKTLLA RAVAGEAGVP FFTISGSDFV EMFVGVGASR VRDLFEQAKQ NSPCIIFVDE IDAVGRQRGA GLGGGHDERE QTLNQLLVEM DGFGDRQGVI LIAATNRPDI LDPALLRPGR FDRQIPVSSP DLAGRKAVLX VHSAGKPFGP DVDFXGLAKR TVXMSGAXLA NVINXAALLT ARENXTVITA AALXXSVDRX VGGPXXXGRI ISEHEXXXXX XXXXXXRXXX XXXXXXXVXX XXXXARGRXX GHAIAXPEDD KXXATXXEMI AQLVFAMGGR AAEELVFREP TTGAVSDIEQ ATKKARAMVT EFGMSAKLGA VRYGTEHGDP FLGRTMGTQA DYSHEVAREI DEEVRNLIEA AHTEAWAILT EYRDVLDTLA GALLEKETVV RKELEEIFSG VQRRPRLTMF DDFGGRIPSD KPPIKTPGEL AIERGEPWPP PVPEPAFKKA IAEQAAAAAP ANGVPPAPAG PHGHLPNGQG GQPVPPGVNY GAPAGWHAPG WPPPGQAPHP QPGYQPYPPQ PYPYPVPTPH QQPGPA

Validated NT Sequence

gagccgggcc cggctgctga tgcggagtcg gcaccgggta cgggtagggc tgcggcggat atggctgata gcccggctgc gggtgcggag cctgcccggg gggaggccag ccgggtgcgt gccagccggc gggagcccca tagttgactc caggcggaac gggctgtcca ccttggccgt tggggagatg tccatgcgga cccgcaggcg ccggtggcac cccattggcg ggcgccgccg cagccgcctg ctcggcgatg gccttcttga acgcgggctc cgggaccggt gggggccagg gttcgccacg ttcgatagca agctcgccgg gcgtcttgat gggcggcttg tcggagggga tacgaccgcc aaagtcgtcg aacatggtca accgcggacg gcgctggacg ccggagaaga tttcctcgag ttccttacgg accaccgttt ccttctccag cagtgcgccg gccagggtgt ccagcacatc ccggtactcg gtgaggatcg cccatgcctc ggtgtgcgcc gcctcgatga gattgcgcac ctcctcatcg atttcgcggg cgacctcatg ggagtaatca gcctgggtgc ccatcgtgcg gcccaggaac ggatcaccgt gttcagtgcc gtagcgcacc gctccgagct tggcgctcat cccgaactcg gtgaccatcg cacgggcctt cttggtggcc tgttcgatat cggacaccgc accggtggtc ggctcccgga acacgagttc ttcggccgcc cggccgccca tcgcgaanac cagctgcgcg atcatttccn anctggtggc cnnnnccttg tcatcctcgg gaancgctat ggcgtgtccn cnnntacgcc cacgcgccna ganggncanc nnntacaccc nctnnnnntc ngnnatnnnc nnnnannngc gtgnnnnnng ccnnnnnata ngcgnnannn tnttttcgtg ctcgctgatg atgcgcccct ngngangggg accaccaacg nccctgtcga cngantcntc cagcgcggca gcngtgatca cggtgcngtt ctcacgggcc gtgagcaacg ccgcntcgtt gatcacgttg gcaaggtngg caccggacat gcngacggtg cgcttggcca gnccatngaa gtcgacatcg gggccgaagg gcttgcccgc cgaatggact ntgagcacgg ccttgcggcc cgccaggtcg gggctggaca ccgggatctg ccggtcgaaa cgaccgggac gcaacagtgc cggatccagg atgtcggggc ggttggtcgc ggcgatcagg atgacaccct gccggtcacc gaagccatcc atctccacca acagctggtt cagggtctgt tcacgctcgt cgtgcccacc gcccaggccg gcgccacgct gccgtccgac tgcgtcgatc tcgtcgacga aaatgatgca gggactgttc tgcttggcct gctcaaacag gtcacgcacg cgcgaagcgc caacgcccac aaacatttcc acgaagtcgg agccggagat ggtaaagaac ggcactcctg cctcaccggc gaccgcacgg gccaacagcg tcttaccggt gccgggcggg ccatagagca gcacgccacg cgggatcttg gcaccgagcg cctgataccg cgacgggttc tgcaggaagt ccttgatctc gtagagctcc tcgaccgcct cgtcgacacc tgccacgtcg gcgaaggtgg tcttgggcat gtccttggac agctgcttgg cacgcgattt accgaaaccg aatcccatgc cccggccccc ggactgcatc c

Expected Protein Sequence

MAHHHHHHSR MQSGGRGMGF GFGKSRAKQL SKDMPKTTFA DVAGVDEAVE ELYEIKDFLQ NPSRYQALGA KIPRGVLLYG PPGTGKTLLA RAVAGEAGVP FFTISGSDFV EMFVGVGASR VRDLFEQAKQ NSPCIIFVDE IDAVGRQRGA GLGGGHDERE QTLNQLLVEM DGFGDRQGVI LIAATNRPDI LDPALLRPGR FDRQIPVSSP DLAGRKAVLK VHSAGKPFGP DVDFDGLAKR TVGMSGADLA NVINEAALLT ARENGTVITA AALEESVDRV VGGPRRKGRI ISEHEKKITA YHEAGHTLAA WAMPDIERVY KVTILARGRT GGHAIAVPED DKGLATRSEM IAQLVFAMGG RAAEELVFRE PTTGAVSDIE QATKKARAMV TEFGMSAKLG AVRYGTEHGD PFLGRTMGTQ ADYSHEVARE IDEEVRNLIE AAHTEAWAIL TEYRDVLDTL AGALLEKETV VRKELEEIFS GVQRRPRLTM FDDFGGRIPS DKPPIKTPGE LAIERGEPWP PPVPEPAFKK AIAEQAAAAA PANGVPPAPA GPHGHLPNGQ GGQPVPPGVN YGAPAGWHAP GWPPPGQAPH PQPGYQPYPP QPYPYPVPTP HQQPGPAPDE GSESKG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagccgga tgcagtccgg gggccggggc atgggattcg gtttcggtaa atcgcgtgcc aagcagctgt ccaaggacat gcccaagacc accttcgccg acgtggcagg tgtcgacgag gcggtcgagg agctctacga gatcaaggac ttcctgcaga acccgtcgcg gtatcaggcg ctcggtgcca agatcccgcg tggcgtgctg ctctatggcc cgcccggcac cggtaagacg ctgttggccc gtgcggtcgc cggtgaggca ggagtgccgt tctttaccat ctccggctcc gacttcgtgg aaatgtttgt gggcgttggc gcttcgcgcg tgcgtgacct gtttgagcag gccaagcaga acagtccctg catcattttc gtcgacgaga tcgacgcagt cggacggcag cgtggcgccg gcctgggcgg tgggcacgac gagcgtgaac agaccctgaa ccagctgttg gtggagatgg atggcttcgg tgaccggcag ggtgtcatcc tgatcgccgc gaccaaccgc cccgacatcc tggatccggc actgttgcgt cccggtcgtt tcgaccggca gatcccggtg tccagccccg acctggcggg ccgcaaggcc gtgctcaaag tccattcggc gggcaagccc ttcggccccg atgtcgactt cgatggcctg gccaagcgca ccgtcggcat gtccggtgcc gaccttgcca acgtgatcaa cgaggcggcg ttgctcacgg cccgtgagaa cggcaccgtg atcacggctg ccgcgctgga ggagtcggtc gacagggtcg ttggtggtcc ccgtcgcaag gggcgcatca tcagcgagca cgaaaagaag atcaccgcgt atcacgaggc cggccacacg ctggctgcct gggccatgcc cgatatcgag cgggtgtaca aggtgaccat cttggcgcgt gggcgtaccg gcggacacgc catagcggtt cccgaggatg acaagggcct ggccaccagg tcggaaatga tcgcgcagct ggtcttcgcg atgggcggcc gggcggccga agaactcgtg ttccgggagc cgaccaccgg tgcggtgtcc gatatcgaac aggccaccaa gaaggcccgt gcgatggtca ccgagttcgg gatgagcgcc aagctcggag cggtgcgcta cggcactgaa cacggtgatc cgttcctggg ccgcacgatg ggcacccagg ctgattactc ccatgaggtc gcccgcgaaa tcgatgagga ggtgcgcaat ctcatcgagg cggcgcacac cgaggcatgg gcgatcctca ccgagtaccg ggatgtgctg gacaccctgg ccggcgcact gctggagaag gaaacggtgg tccgtaagga actcgaggaa atcttctccg gcgtccagcg ccgtccgcgg ttgaccatgt tcgacgactt tggcggtcgt atcccctccg acaagccgcc catcaagacg cccggcgagc ttgctatcga acgtggcgaa ccctggcccc caccggtccc ggagcccgcg ttcaagaagg ccatcgccga gcaggcggct gcggcggcgc ccgccaatgg ggtgccaccg gcgcctgcgg gtccgcatgg acatctcccc aacggccaag gtggacagcc cgttccgcct ggagtcaact atggggctcc cgccggctgg cacgcacccg gctggcctcc ccccgggcag gctccgcacc cgcagccggg ctatcagcca tatccgccgc agccctaccc gtacccggtg ccgactccgc atcagcagcc gggcccggct ccggacgagg gcagcgagag caagggctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at