SSGCID: Target Page

MyabA.17861.q

DNA-directed RNA polymerase (Rv0667 ortholog)

CENTER ID:	MyabA.17861.q
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.17861.q.B1.GE37324	full length		1	204

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	B2LYU0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

IFGEKAREVR DTSLKVPHGE SGKVIGIRVF SRDDDDDLPA GVNELVRVYV AQKRKISDGD KLAGRHGNKG VIGKILPVED MPFLPDGTPV DIILNTHGVP RRMNIGQILE THLGWIAKTG WNIEGEPEWA ANLPADLTSA PADTRTATPV FDGAREEELT GLLSSTLPNR DGEVMVDGDG KARLFDGRSG EPFPYPVTVG YMYI

NT Sequence

ATCTTCGGTG AGAAGGCTCG CGAGGTCCGC GACACCTCCC TGAAGGTGCC GCACGGCGAG TCCGGAAAGG TCATCGGCAT CCGCGTGTTC TCGCGTGATG ACGACGACGA TCTGCCTGCC GGCGTGAATG AGCTCGTTCG CGTGTACGTG GCGCAGAAGC GCAAGATCTC CGACGGTGAC AAGCTGGCCG GACGCCACGG CAACAAGGGC GTCATCGGCA AGATCCTGCC CGTCGAGGAC ATGCCGTTCC TGCCCGATGG CACCCCGGTG GACATCATCC TGAACACCCA CGGTGTGCCA CGCCGTATGA ACATCGGCCA GATCTTGGAA ACCCACCTCG GGTGGATTGC CAAGACCGGC TGGAACATCG AGGGTGAGCC CGAGTGGGCG GCCAATCTGC CTGCAGACCT CACCTCTGCG CCGGCCGATA CCCGCACGGC CACCCCGGTG TTCGACGGTG CCCGCGAGGA GGAGCTGACC GGACTGCTGT CCTCGACGCT GCCCAACCGG GACGGCGAGG TCATGGTGGA TGGTGACGGC AAGGCGCGGT TGTTCGACGG CCGGAGCGGC GAGCCGTTCC CGTACCCGGT GACCGTCGGT TACATGTACA TC

Details for MyabA.17861.q.B1.GE37324

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	23kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMI FGEKAREVRD TSLKVPHGES GKVIGIRVFS RDDDDDLPAG VNELVRVYVA QKRKISDGDK LAGRHGNKGV IGKILPVEDM PFLPDGTPVD IILNTHGVPR RMNIGQILET HLGWIAKTGW NIEGEPEWAA NLPADLTSAP ADTRTATPVF DGAREEELTG LLSSTLPNRD GEVMVDGDGK ARLFDGRSGE PFPYPVTVGY MYI

Validated NT Sequence

gcagccggat cctatcttac tcacttagat gtacatgtaa ccgacggtca ccgggtacgg gaacggctcg ccgctccggc cgtcgaacaa ccgcgccttg ccgtcaccat ccaccatgac ctcgccgtcc cggttgggca gcgtcgagga cagcagtccg gtcagctcct cctcgcgggc accgtcgaac accggggtgg ccgtgcgggt atcggccggc gcagaggtga ggtctgcagg cagattggcc gcccactcgg gctcaccctc gatgttccag ccggtcttgg caatccaccc gaggtgggtt tccaagatct ggccgatgtt catacggcgt ggcacaccgt gggtgttcag gatgatgtcc accggggtgc catcgggcag gaacggcatg tcctcgacgg gcaggatctt gccgatgacg cccttgttgc cgtggcgtcc ggccagcttg tcaccgtcgg agatcttgcg cttctgcgcc acgtacacgc gaacgagctc attcacgccg gcaggcagat cgtcgtcgtc atcacgcgag aacacgcgga tgccgatgac ctttccggac tcgccgtgcg gcaccttcag ggaggtgtcg cggacctcgc gagccttctc accgaagatc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHIF GEKAREVRDT SLKVPHGESG KVIGIRVFSR DDDDDLPAGV NELVRVYVAQ KRKISDGDKL AGRHGNKGVI GKILPVEDMP FLPDGTPVDI ILNTHGVPRR MNIGQILETH LGWIAKTGWN IEGEPEWAAN LPADLTSAPA DTRTATPVFD GAREEELTGL LSSTLPNRDG EVMVDGDGKA RLFDGRSGEP FPYPVTVGYM YI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatcttcg gtgagaaggc tcgcgaggtc cgcgacacct ccctgaaggt gccgcacggc gagtccggaa aggtcatcgg catccgcgtg ttctcgcgtg atgacgacga cgatctgcct gccggcgtga atgagctcgt tcgcgtgtac gtggcgcaga agcgcaagat ctccgacggt gacaagctgg ccggacgcca cggcaacaag ggcgtcatcg gcaagatcct gcccgtcgag gacatgccgt tcctgcccga tggcaccccg gtggacatca tcctgaacac ccacggtgtg ccacgccgta tgaacatcgg ccagatcttg gaaacccacc tcgggtggat tgccaagacc ggctggaaca tcgagggtga gcccgagtgg gcggccaatc tgcctgcaga cctcacctct gcgccggccg atacccgcac ggccaccccg gtgttcgacg gtgcccgcga ggaggagctg accggactgc tgtcctcgac gctgcccaac cgggacggcg aggtcatggt ggatggtgac ggcaaggcgc ggttgttcga cggccggagc ggcgagccgt tcccgtaccc ggtgaccgtc ggttacatgt acatctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat