SSGCID: Target Page

MyabA.17861.p

DNA-directed RNA polymerase (Rv0667 ortholog)

CENTER ID:	MyabA.17861.p
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.17861.p.B11.GE37619	full length		1	220

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	G9HVI9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

REVRDTSLKV PHGESGKVIG IRVFSRDDDD DLPAGVNELV RVYVAQKRKI SDGDKLAGRH GNKGVIGKIL PVEDMPFLPD GTPVDIILNT HGVPRRMNIG QILETHLGWI AKTGWNIEGE PEWAANLPAD LTSAPADTRT ATPVFDGARE EELTGLLSST LPNRDGEVMV DGDGKARLFD GRSGEPFPYP VTVGYMYILK LHHLVDDKIH ARSTXPYSMI

NT Sequence

CGCGAGGTCC GCGACACCTC CCTGAAGGTG CCGCACGGCG AGTCCGGAAA GGTCATCGGC ATCCGCGTGT TCTCGCGTGA TGACGACGAC GATCTGCCTG CCGGCGTGAA TGAGCTCGTT CGCGTGTACG TGGCGCAGAA GCGCAAGATC TCCGACGGTG ACAAGCTGGC CGGACGCCAC GGCAACAAGG GCGTCATCGG CAAGATCCTG CCCGTCGAGG ACATGCCGTT CCTGCCCGAT GGCACCCCGG TGGACATCAT CCTGAACACC CACGGTGTGC CACGCCGTAT GAACATCGGC CAGATCTTGG AAACCCACCT CGGGTGGATT GCCAAGACCG GCTGGAACAT CGAGGGTGAG CCCGAGTGGG CGGCCAATCT GCCTGCAGAC CTCACCTCTG CGCCGGCCGA TACCCGCACG GCCACCCCGG TGTTCGACGG TGCCCGCGAG GAGGAGCTGA CCGGACTGCT GTCCTCGACG CTGCCCAACC GGGACGGCGA GGTCATGGTG GATGGTGACG GCAAGGCGCG GTTGTTCGAC GGCCGGAGCG GCGAGCCGTT CCCGTACCCG GTGACCGTCG GTTACATGTA CATCCTGAAG CTGCACCACT TGGTCGACGA CAAGATCCAC GCGCGTTCGA CCNGCCCGTA CTCGATGATC

Details for MyabA.17861.p.B11.GE37619

HARVESTED ON:	1/13/2014
SEQUENCED ON:	1/9/2014
EXPECTED MW:	25kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR EVRDTSLKVP HGESGKVIGI RVFSRDDDDD LPAGVNELVR VYVAQKRKIS DGDKLAGRHG NKGVIGKILP VEDMPFLPDG TPVDIILNTH GVPRRMNIGQ ILETHLGWIA KTGWNIEGEP EWAANLPADL TSAPADTRTA TPVFDGAREE ELTGLLSSTL PNRDGEVMVD GDGKARLFDG RSGEPFPYPV TVGYMYILKL HHLVDDKIHA RSTGPYSMI

Validated NT Sequence

atcttactca cttagatcat cgagtacggg ccggtcgaac gcgcgtggat cttgtcgtcg accaagtggt gcagcttcag gatgtacatg taaccgacgg tcaccgggta cgggaacggc tcgccgctcc ggccgtcgaa caaccgcgcc ttgccgtcac catccaccat gacctcgccg tcccggttgg gcagcgtcga ggacagcagt ccggtcagct cctcctcgcg ggcaccgtcg aacaccgggg tggccgtgcg ggtatcggcc ggcgcagagg tgaggtctgc aggcagattg gccgcccact cgggctcacc ctcgatgttc cagccggtct tggcaatcca cccgaggtgg gtttccaaga tctggccgat gttcatacgg cgtggcacac cgtgggtgtt caggatgatg tccaccgggg tgccatcggg caggaacggc atgtcctcga cgggcaggat cttgccgatg acgcccttgt tgccgtggcg tccggccagc ttgtcaccgt cggagatctt gcgcttctgc gccacgtaca cgcgaacgag ctcattcacg ccggcaggca gatcgtcgtc gtcatcacgc gagaacacgc ggatgccgat gacctttccg gactcgccgt gcggcacctt cagggaggtg tcgcggacct cgcgcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHRE VRDTSLKVPH GESGKVIGIR VFSRDDDDDL PAGVNELVRV YVAQKRKISD GDKLAGRHGN KGVIGKILPV EDMPFLPDGT PVDIILNTHG VPRRMNIGQI LETHLGWIAK TGWNIEGEPE WAANLPADLT SAPADTRTAT PVFDGAREEE LTGLLSSTLP NRDGEVMVDG DGKARLFDGR SGEPFPYPVT VGYMYILKLH HLVDDKIHAR STXPYSMI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcgagg tccgcgacac ctccctgaag gtgccgcacg gcgagtccgg aaaggtcatc ggcatccgcg tgttctcgcg tgatgacgac gacgatctgc ctgccggcgt gaatgagctc gttcgcgtgt acgtggcgca gaagcgcaag atctccgacg gtgacaagct ggccggacgc cacggcaaca agggcgtcat cggcaagatc ctgcccgtcg aggacatgcc gttcctgccc gatggcaccc cggtggacat catcctgaac acccacggtg tgccacgccg tatgaacatc ggccagatct tggaaaccca cctcgggtgg attgccaaga ccggctggaa catcgagggt gagcccgagt gggcggccaa tctgcctgca gacctcacct ctgcgccggc cgatacccgc acggccaccc cggtgttcga cggtgcccgc gaggaggagc tgaccggact gctgtcctcg acgctgccca accgggacgg cgaggtcatg gtggatggtg acggcaaggc gcggttgttc gacggccgga gcggcgagcc gttcccgtac ccggtgaccg tcggttacat gtacatcctg aagctgcacc acttggtcga cgacaagatc cacgcgcgtt cgaccngccc gtactcgatg atctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat