MyabA.17861.f

DNA-directed RNA polymerase (Rv0667 ortholog)

CENTER ID: MyabA.17861.f
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.17861.f.B1.GE37313 full length 1 247
MyabA.17861.f.B11.GE37614 full length 1 247
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: G0ZTL7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
GKVTPKGETE LTPEERLLRA IFGEKAREVR DTSLKVPHGE SGKVIGIRVF SRDDDDDLPA GVNELVRVYV AQKRKISDGD KLAGRHGNKG VIGKILPVED MPFLPDGTPV DIILNTHGVP RRMNIGQILE THLGWIAKTG WNIEGEPEWA ANLPADLTSA PADTRTATPV FDGAREEELT GLLSSTLPNR DGEVMVDGDG KARLFDGRSG EPFPYPVTVG YMYILKLHHL VDDKIHARST GPYSMIT
NT Sequence
GGCAAGGTCA CCCCGAAGGG TGAGACCGAG CTGACCCCCG AGGAGCGCCT GCTGCGTGCC ATCTTCGGTG AGAAGGCTCG CGAGGTCCGC GACACCTCCC TGAAGGTGCC GCACGGCGAG TCCGGAAAGG TCATCGGCAT CCGCGTGTTC TCGCGTGATG ACGACGACGA TCTGCCTGCC GGCGTGAATG AGCTCGTTCG CGTGTACGTG GCGCAGAAGC GCAAGATCTC CGACGGTGAC AAGCTGGCCG GACGCCACGG CAACAAGGGC GTCATCGGCA AGATCCTGCC CGTCGAGGAC ATGCCGTTCC TGCCCGATGG CACCCCGGTG GACATCATCC TGAACACCCA CGGTGTGCCA CGCCGTATGA ACATCGGCCA GATCTTGGAA ACCCACCTCG GGTGGATTGC CAAGACCGGC TGGAACATCG AGGGTGAGCC CGAGTGGGCG GCCAATCTGC CTGCAGACCT CACCTCTGCG CCGGCCGATA CCCGCACGGC CACCCCGGTG TTCGACGGTG CCCGCGAGGA GGAGCTGACC GGACTGCTGT CCTCGACGCT GCCCAACCGG GACGGCGAGG TCATGGTGGA TGGTGACGGC AAGGCGCGGT TGTTCGACGG CCGGAGCGGC GAGCCGTTCC CGTACCCGGT GACCGTCGGT TACATGTACA TCCTGAAGCT GCACCACTTG GTCGACGACA AGATCCACGC GCGTTCGACC GGCCCGTACT CGATGATCAC C
Details for MyabA.17861.f.B1.GE37313
HARVESTED ON: 8/28/2013
SEQUENCED ON: 9/3/2013
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG KVTPKGETEL TPEERLLRAI FGEKAREVRD TSLKVPHGES GKVIGIRVFS RDDDDDLPAG VNELVRVYVA QKRKISDGDK LAGRHGNKGV IGKILPVEDM PFLPDGTPVD IILNTHGVPR RMNIGQILET HLGWIAKTGW NIEGEPEWAA NLPADLTSAP ADTRTATPVF DGAREEELTG LLSSTLPNRD GEVMVDGDGK ARLFDGRSGE PFPYPVTVGY MYILKLHHLV DDXIHARSTG PYSMIT
Validated NT Sequence
cnnnnnattt tntttacttt aagaaggaga tataccatgg ctcaccacca ccaccaccat atgggcaagg tcaccccgaa gggtgagacc gagctgaccc ccgaggagcg cctgctgcgt gccatcttcg gtgagaaggc tcgcgaggtc cgcgacacct ccctgaaggt gccgcacggc gagtccggaa aggtcatcgg catccgcgtg ttctcgcgtg atgacgacga cgatctgcct gccggcgtga atgagctcgt tcgcgtgtac gtggcgcaga agcgcaagat ctccgacggt gacaagctgg ccggacgcca cggcaacaag ggcgtcatcg gcaagatcct gcccgtcgag gacatgccgt tcctgcccga tggcaccccg gtggacatca tcctgaacac ccacggtgtg ccacgccgta tgaacatcgg ccagatcttg gaaacccacc tcgggtggat tgccaagacc ggctggaaca tcgagggtga gcccgagtgg gcggccaatc tgcctgcaga cctcacctct gcgccggccg atacccgcac ggccaccccg gtgttcgacg gtgcccgcga ggaggagctg accggactgc tgtcctcgac gctgcccaac cgggacggcg aggtcatggt ggatggtgac ggcaaggcgc ggttgttcga cggccggagc ggcgagccgt tcccgtaccc ggtgaccgtc ggttacatgt acatcctgaa gctgcaccac ttggtcgacg acnagatcca cgcgcgttcg accggcccgt actcgatgat cacctaagtg agtaanatag gatccggntn nnnnnnnccc cnnnnnnnnn naannnnnnn nnnnnnnnnt nncnnnnnnn nnnnnnncgg tccacangnc ncnnntggtg gnnccccntn nncgagnggn nnatnntnng nnnngcacga annngaanat ccncnnntan tncccnngnc cngnncncnc nntg
Expected Protein Sequence
MAHHHHHHGK VTPKGETELT PEERLLRAIF GEKAREVRDT SLKVPHGESG KVIGIRVFSR DDDDDLPAGV NELVRVYVAQ KRKISDGDKL AGRHGNKGVI GKILPVEDMP FLPDGTPVDI ILNTHGVPRR MNIGQILETH LGWIAKTGWN IEGEPEWAAN LPADLTSAPA DTRTATPVFD GAREEELTGL LSSTLPNRDG EVMVDGDGKA RLFDGRSGEP FPYPVTVGYM YILKLHHLVD DKIHARSTGP YSMIT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggcaagg tcaccccgaa gggtgagacc gagctgaccc ccgaggagcg cctgctgcgt gccatcttcg gtgagaaggc tcgcgaggtc cgcgacacct ccctgaaggt gccgcacggc gagtccggaa aggtcatcgg catccgcgtg ttctcgcgtg atgacgacga cgatctgcct gccggcgtga atgagctcgt tcgcgtgtac gtggcgcaga agcgcaagat ctccgacggt gacaagctgg ccggacgcca cggcaacaag ggcgtcatcg gcaagatcct gcccgtcgag gacatgccgt tcctgcccga tggcaccccg gtggacatca tcctgaacac ccacggtgtg ccacgccgta tgaacatcgg ccagatcttg gaaacccacc tcgggtggat tgccaagacc ggctggaaca tcgagggtga gcccgagtgg gcggccaatc tgcctgcaga cctcacctct gcgccggccg atacccgcac ggccaccccg gtgttcgacg gtgcccgcga ggaggagctg accggactgc tgtcctcgac gctgcccaac cgggacggcg aggtcatggt ggatggtgac ggcaaggcgc ggttgttcga cggccggagc ggcgagccgt tcccgtaccc ggtgaccgtc ggttacatgt acatcctgaa gctgcaccac ttggtcgacg acaagatcca cgcgcgttcg accggcccgt actcgatgat cacctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for MyabA.17861.f.B11.GE37614
HARVESTED ON: 1/13/2014
SEQUENCED ON: 1/9/2014
EXPECTED MW: 28kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG KVTPKGETEL TPEERLLRAI FGEKAREVRD TSLKVPHGES GKVIGIRVFS RDDDDDLPAG VNELVRVYVA QKRKISDGDK LAGRHGNKGV IGKILPVEDM PFLPDGTPVD IILNTHGVPR RMNIGQILET HLGWIAKTGW NIEGEPEWAA NLPADLTSAP ADTRTATPVF DGAREEELTG LLSSTLPNRD GEVMVDGDGK ARLFDGRSGE PFPYPVTVGY MYILKLHHLV DDKIHARSTG PYSMIT
Validated NT Sequence
atcttactca cttaggtgat catcgagtac gggccggtcg aacgcgcgtg gatcttgtcg tcgaccaagt ggtgcagctt caggatgtac atgtaaccga cggtcaccgg gtacgggaac ggctcgccgc tccggccgtc gaacaaccgc gccttgccgt caccatccac catgacctcg ccgtcccggt tgggcagcgt cgaggacagc agtccggtca gctcctcctc gcgggcaccg tcgaacaccg gggtggccgt gcgggtatcg gccggcgcag aggtgaggtc tgcaggcaga ttggccgccc actcgggctc accctcgatg ttccagccgg tcttggcaat ccacccgagg tgggtttcca agatctggcc gatgttcata cggcgtggca caccgtgggt gttcaggatg atgtccaccg gggtgccatc gggcaggaac ggcatgtcct cgacgggcag gatcttgccg atgacgccct tgttgccgtg gcgtccggcc agcttgtcac cgtcggagat cttgcgcttc tgcgccacgt acacgcgaac gagctcattc acgccggcag gcagatcgtc gtcgtcatca cgcgagaaca cgcggatgcc gatgaccttt ccggactcgc cgtgcggcac cttcagggag gtgtcgcgga cctcgcgagc cttctcaccg aagatggcac gcagcaggcg ctcctcgggg gtcagctcgg tctcaccctt cggggtgacc ttgcccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHGK VTPKGETELT PEERLLRAIF GEKAREVRDT SLKVPHGESG KVIGIRVFSR DDDDDLPAGV NELVRVYVAQ KRKISDGDKL AGRHGNKGVI GKILPVEDMP FLPDGTPVDI ILNTHGVPRR MNIGQILETH LGWIAKTGWN IEGEPEWAAN LPADLTSAPA DTRTATPVFD GAREEELTGL LSSTLPNRDG EVMVDGDGKA RLFDGRSGEP FPYPVTVGYM YILKLHHLVD DKIHARSTGP YSMIT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggcaagg tcaccccgaa gggtgagacc gagctgaccc ccgaggagcg cctgctgcgt gccatcttcg gtgagaaggc tcgcgaggtc cgcgacacct ccctgaaggt gccgcacggc gagtccggaa aggtcatcgg catccgcgtg ttctcgcgtg atgacgacga cgatctgcct gccggcgtga atgagctcgt tcgcgtgtac gtggcgcaga agcgcaagat ctccgacggt gacaagctgg ccggacgcca cggcaacaag ggcgtcatcg gcaagatcct gcccgtcgag gacatgccgt tcctgcccga tggcaccccg gtggacatca tcctgaacac ccacggtgtg ccacgccgta tgaacatcgg ccagatcttg gaaacccacc tcgggtggat tgccaagacc ggctggaaca tcgagggtga gcccgagtgg gcggccaatc tgcctgcaga cctcacctct gcgccggccg atacccgcac ggccaccccg gtgttcgacg gtgcccgcga ggaggagctg accggactgc tgtcctcgac gctgcccaac cgggacggcg aggtcatggt ggatggtgac ggcaaggcgc ggttgttcga cggccggagc ggcgagccgt tcccgtaccc ggtgaccgtc ggttacatgt acatcctgaa gctgcaccac ttggtcgacg acaagatcca cgcgcgttcg accggcccgt actcgatgat cacctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat