SSGCID: Target Page

MyabA.17391.a

Alternative sigma factor SigF (Rv3286c ortholog)

CENTER ID:	MyabA.17391.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.17391.a.B1.GE37305	full length		1	272

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.2554
RefSeq:	YP_001703246.1
UniProt:	B1MBH1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MECPVDVPET GHSQVKPRVG RPTDDYADVP DMFRSLAGLA EHSDAYQRKH EEIVTRCLPL ADNVARHFDR RGEDLEDLIQ VARVGLLNAV NRFDPEKGAS FLGFAVPTMM GEVRRHFRDH GWSLHVPRAI RDRHAQISRA TAELTQELQR APTAGELAAE LGIDREEVVE SLIAADAYQP QSIEAPLSHG DQESKHLSDL LGNEDPALEH VTNREAVRPL LANLPLRERT VLELRFFKGM TQSQIAAQIG VSQMHVSRIL SDTLRYLREQ MA

NT Sequence

atggaatgcc ctgttgatgt accggagacc ggacactcac aggtgaaacc acgcgttggc cggccgactg acgactacgc ggacgtaccg gacatgtttc ggtctctcgc cggcttggcg gagcacagtg acgcatacca acgtaagcac gaggagatag tcaccagatg cctgccgctg gccgacaatg tcgcacgtca tttcgatcgc cgtggtgaag atctcgagga cctgatccag gttgcccgag tgggcttgct caatgcggtc aaccgttttg acccggaaaa gggcgccagc ttcctcggct ttgcggttcc taccatgatg ggggaggtgc gccggcactt tcgcgaccac ggatggtcgc tgcacgtacc gcgggctata agagatagac acgctcaaat ctccagggcc actgcggaat tgacccagga gctacagcgg gcgcctaccg ccggggagtt ggcggcagag ttgggtatcg accgcgaaga ggtggtggaa tctctgattg ccgcggacgc ctatcagcct caatccatcg aggcgcccct cagccacggc gatcaggaat ccaagcatct ttccgatctg ctggggaatg aagatccagc cctggaacac gtgaccaacc gcgaggcagt acggcctctt ttagccaatt tacccctgcg agagcgaacc gtgcttgagc tccggttttt caagggaatg acccagagcc agattgccgc acagattggt gtttcgcaga tgcacgtatc ccggattctc agcgacacct tgcggtacct gcgagaacag atggcgtag

Details for MyabA.17391.a.B1.GE37305

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	32kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHME CPVDVPETGH SQVKPRVGRP TDDYADVPDM FRSLAGLAEH SDAYQRKHEE IVTRCLPLAD NVARHFDRRG EDLEDLIQVA RVGLLNAVNR FDPEKGASFL GFAVPTMMGE VRRHFRDHGW SLHVPRAIRD RHAQISRATA ELTQELQRAP TAGELAAELG IDREEVVESL IAADAYQPQS IEAPLSHGDQ ESKHLSDLLG NEDPALEHVT NREAVRPLLA NLPLRERTVL ELRFFKGMTQ SQIAAQIGVS QMHVSRILSD TLRYLREQMA

Validated NT Sequence

atggctcacc accaccacca ccatatggaa tgccctgttg atgtaccgga gaccggacac tcacaggtga aaccacgcgt tggccggccg actgacgact acgcggacgt accggacatg tttcggtctc tcgccggctt ggcggagcac agtgacgcat accaacgtaa gcacgaggag atagtcacca gatgcctgcc gctggccgac aatgtcgcac gtcatttcga tcgccgtggt gaagatctcg aggacctgat ccaggttgcc cgagtgggct tgctcaatgc ggtcaaccgt tttgacccgg aaaagggcgc cagcttcctc ggctttgcgg ttcctaccat gatgggggag gtgcgccggc actttcgcga ccacggatgg tcgctgcacg taccgcgggc tataagagat agacacgctc aaatctccag ggccactgcg gaattgaccc aggagctaca gcgggcgcct accgccgggg agttggcggc agagttgggt atcgaccgcg aagaggtggt ggaatctctg attgccgcgg acgcctatca gcctcaatcc atcgaggcgc ccctcagcca cggcgatcag gaatccaagc atctttccga tctgctgggg aatgaagatc cagccctgga acacgtgacc aaccgcgagg cagtacggcc tcttttagcc aatttacccc tgcgagagcg aaccgtgctt gagctccggt ttttcaaggg aatgacccag agccagattg ccgcacagat tggtgtttcg cagatgcacg tatcccggat tctcagcgac accttgcggt acctgcgaga acagatggcg taagtgagta agat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaat gccctgttga tgtaccggag accggacact cacaggtgaa accacgcgtt ggccggccga ctgacgacta cgcggacgta ccggacatgt ttcggtctct cgccggcttg gcggagcaca gtgacgcata ccaacgtaag cacgaggaga tagtcaccag atgcctgccg ctggccgaca atgtcgcacg tcatttcgat cgccgtggtg aagatctcga ggacctgatc caggttgccc gagtgggctt gctcaatgcg gtcaaccgtt ttgacccgga aaagggcgcc agcttcctcg gctttgcggt tcctaccatg atgggggagg tgcgccggca ctttcgcgac cacggatggt cgctgcacgt accgcgggct ataagagata gacacgctca aatctccagg gccactgcgg aattgaccca ggagctacag cgggcgccta ccgccgggga gttggcggca gagttgggta tcgaccgcga agaggtggtg gaatctctga ttgccgcgga cgcctatcag cctcaatcca tcgaggcgcc cctcagccac ggcgatcagg aatccaagca tctttccgat ctgctgggga atgaagatcc agccctggaa cacgtgacca accgcgaggc agtacggcct cttttagcca atttacccct gcgagagcga accgtgcttg agctccggtt tttcaaggga atgacccaga gccagattgc cgcacagatt ggtgtttcgc agatgcacgt atcccggatt ctcagcgaca ccttgcggta cctgcgagaa cagatggcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat