SSGCID: Target Page

MyabA.11416.a

Putative chromosome partitioning protein/ cobyrinic acid a,c-diamide synthase (Rv3918c ortholog)

CENTER ID:	MyabA.11416.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.11416.a.B1.GE37303	full length		1	314

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.11416.a.B1.PS01963	full length		1	314

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.5032
RefSeq:	YP_001705672.1
UniProt:	B1MN92

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSDDVSRET GAASDGHRAP TEFVESETPI GAAAQRATQL KQGSVRLPKP AHRRMLTIAN QKGGVGKTTT AVNLAAAMAL QGLNVLVIDL DPQGNASTAL GADHRAGTPS SYEVLLGEIP IQDAIQSSPQ SEHLFCVPAT IDLAGAEIEL VSMVAREGRL RSAIAGLPAD AFDFVFIDCP PSLGLLTVNA LVAANEVLIP IQCEYYALEG VGQLLRNIEL VQAHLNPALH VSTILLTMYD GRTKLADQVA DEVRGHFGPK VLGSVIPRSV KVSEAPGYGT SVLEYDPGSR GALSYLDAAR ELAQRPSSSA SDPQ

NT Sequence

gtgagtagcg acgatgtttc acgtgaaacc ggcgcagcga gtgacggcca ccgggcgccc acggagttcg tggaatccga gactcccatc ggcgccgcgg cgcagcgagc gactcagttg aagcaaggct cggtccgact accgaagccc gcacatcggc ggatgctgac gatcgccaat cagaagggcg gcgtcggcaa gaccaccact gcggtgaacc ttgcggcggc gatggcactg caggggctca atgttctggt gatcgatttg gacccccaag gcaacgcaag caccgcactg ggtgccgacc accgcgcggg caccccctcg tcgtacgagg tactccttgg cgagataccc attcaggacg cgattcagtc cagcccgcag agcgagcacc tcttctgcgt tccggcgacg atcgaccttg ccggtgccga gatcgagctg gtttcgatgg tcgcgcgcga agggcggttg cgttcggcga tcgccggtct cccggccgat gcgttcgact tcgtcttcat tgattgcccg ccatcgctcg gtctgttgac ggtcaatgcg ctcgttgccg ccaacgaagt gttgatcccc atccagtgcg agtactacgc attggaaggg gtgggccagc tgctgcgcaa catcgaactt gtccaagcac atctcaaccc ggcgctgcat gtgtcgacga tcttgctcac catgtatgac ggccggacca agctggccga ccaagtggcg gatgaggtgc ggggccactt cggcccgaag gtactgggtt cggtcatccc gcgtagcgtc aaggtgtcgg aagcaccggg gtacggaacc agtgttcttg aatacgatcc gggttcacgt ggcgcgttga gctatctcga tgcagcgcgc gaactcgccc agcggccgag ctcgtcggcg agtgatccgc aatga

Details for MyabA.11416.a.B1.GE37303

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	34kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Validated AA Sequence

MVSSDDVSRE TGAASDGHRA PTEFVESETP IGAAAQRATQ LKQGSVRLPK PAHRRMLTIA NQKGGVGKTT TAVNLAAAMA LQGLNVLVID LDPQGNASTA LGADHRAGTP SSYEVLLGEI PIQDAIQSSP QSEHLFCVPA TIDLAGAEIE LVSMVAREGR LRSAIAGLPA DAFDFVFIDC PPSLGLLTVN ALVAANEVLI PIQCEYYALE GVGQLLRNIE LVQAHLNPAL HVSTILLTMY DGRTKLADQV ADEVRGHFGP KVLGSVIPRS VKVSEAPGYG TSVLEYDPGS RGALSYLDAA RELAQRPSS

Validated NT Sequence

acgagctcgg ccgctgggcg agttcgcgcg ctgcatcgag atagctcaac gcgccacgtg aacccggatc gtattcaaga acactggttc cgtaccccgg tgcttccgac accttgacgc tacgcgggat gaccgaaccc agtaccttcg ggccgaagtg gccccgcacc tcatccgcca cttggtcggc cagcttggtc cggccgtcat acatggtgag caagatcgtc gacacatgca gcgccgggtt gagatgtgct tggacaagtt cgatgttgcg cagcagctgg cccacccctt ccaatgcgta gtactcgcac tggatgggga tcaacacttc gttggcggca acgagcgcat tgaccgtcaa cagaccgagc gatggcgggc aatcaatgaa gacgaagtcg aacgcatcgg ccgggagacc ggcgatcgcc gaacgcaacc gcccttcgcg cgcgaccatc gaaaccagct cgatctcggc accggcaagg tcgatcgtcg ccggaacgca gaagaggtgc tcgctctgcg ggctggactg aatcgcgtcc tgaatgggta tctcgccaag gagtacctcg tacgacgagg gggtgcccgc gcggtggtcg gcacccagtg cggtgcttgc gttgccttgg gggtccaaat cgatcaccag aacattgagc ccctgcagtg ccatcgccgc cgcaaggttc accgcagtgg tggtcttgcc gacgccgccc ttctgattgg cgatcgtcag catccgccga tgtgcgggct tcggtagtcg gaccgagcct tgcttcaact gagtcgctcg ctgcgccgcg gcgccgatgg gagtctcgga ttccacgaac tccgtgggcg cccggtggcc gtcactcgct gcgccggttt cacgtgaaac atcgtcgcta ctcaccatat ggtggtggtg gtg

Expected Protein Sequence

MAHHHHHHMS SDDVSRETGA ASDGHRAPTE FVESETPIGA AAQRATQLKQ GSVRLPKPAH RRMLTIANQK GGVGKTTTAV NLAAAMALQG LNVLVIDLDP QGNASTALGA DHRAGTPSSY EVLLGEIPIQ DAIQSSPQSE HLFCVPATID LAGAEIELVS MVAREGRLRS AIAGLPADAF DFVFIDCPPS LGLLTVNALV AANEVLIPIQ CEYYALEGVG QLLRNIELVQ AHLNPALHVS TILLTMYDGR TKLADQVADE VRGHFGPKVL GSVIPRSVKV SEAPGYGTSV LEYDPGSRGA LSYLDAAREL AQRPSSSASD PQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagta gcgacgatgt ttcacgtgaa accggcgcag cgagtgacgg ccaccgggcg cccacggagt tcgtggaatc cgagactccc atcggcgccg cggcgcagcg agcgactcag ttgaagcaag gctcggtccg actaccgaag cccgcacatc ggcggatgct gacgatcgcc aatcagaagg gcggcgtcgg caagaccacc actgcggtga accttgcggc ggcgatggca ctgcaggggc tcaatgttct ggtgatcgat ttggaccccc aaggcaacgc aagcaccgca ctgggtgccg accaccgcgc gggcaccccc tcgtcgtacg aggtactcct tggcgagata cccattcagg acgcgattca gtccagcccg cagagcgagc acctcttctg cgttccggcg acgatcgacc ttgccggtgc cgagatcgag ctggtttcga tggtcgcgcg cgaagggcgg ttgcgttcgg cgatcgccgg tctcccggcc gatgcgttcg acttcgtctt cattgattgc ccgccatcgc tcggtctgtt gacggtcaat gcgctcgttg ccgccaacga agtgttgatc cccatccagt gcgagtacta cgcattggaa ggggtgggcc agctgctgcg caacatcgaa cttgtccaag cacatctcaa cccggcgctg catgtgtcga cgatcttgct caccatgtat gacggccgga ccaagctggc cgaccaagtg gcggatgagg tgcggggcca cttcggcccg aaggtactgg gttcggtcat cccgcgtagc gtcaaggtgt cggaagcacc ggggtacgga accagtgttc ttgaatacga tccgggttca cgtggcgcgt tgagctatct cgatgcagcg cgcgaactcg cccagcggcc gagctcgtcg gcgagtgatc cgcaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for MyabA.11416.a.B1.PS01963

PURIFICATION DATe:	1/3/2014
CONCENTRATION:	11.96mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAGTA GCGACGATGT TTCACGTGAA ACCGGCGCAG CGAGTGACGG CCACCGGGCG CCCACGGAGT TCGTGGAATC CGAGACTCCC ATCGGCGCCG CGGCGCAGCG AGCGACTCAG TTGAAGCAAG GCTCGGTCCG ACTACCGAAG CCCGCACATC GGCGGATGCT GACGATCGCC AATCAGAAGG GCGGCGTCGG CAAGACCACC ACTGCGGTGA ACCTTGCGGC GGCGATGGCA CTGCAGGGGC TCAATGTTCT GGTGATCGAT TTGGACCCCC AAGGCAACGC AAGCACCGCA CTGGGTGCCG ACCACCGCGC GGGCACCCCC TCGTCGTACG AGGTACTCCT TGGCGAGATA CCCATTCAGG ACGCGATTCA GTCCAGCCCG CAGAGCGAGC ACCTCTTCTG CGTTCCGGCG ACGATCGACC TTGCCGGTGC CGAGATCGAG CTGGTTTCGA TGGTCGCGCG CGAAGGGCGG TTGCGTTCGG CGATCGCCGG TCTCCCGGCC GATGCGTTCG ACTTCGTCTT CATTGATTGC CCGCCATCGC TCGGTCTGTT GACGGTCAAT GCGCTCGTTG CCGCCAACGA AGTGTTGATC CCCATCCAGT GCGAGTACTA CGCATTGGAA GGGGTGGGCC AGCTGCTGCG CAACATCGAA CTTGTCCAAG CACATCTCAA CCCGGCGCTG CATGTGTCGA CGATCTTGCT CACCATGTAT GACGGCCGGA CCAAGCTGGC CGACCAAGTG GCGGATGAGG TGCGGGGCCA CTTCGGCCCG AAGGTACTGG GTTCGGTCAT CCCGCGTAGC GTCAAGGTGT CGGAAGCACC GGGGTACGGA ACCAGTGTTC TTGAATACGA TCCGGGTTCA CGTGGCGCGT TGAGCTATCT CGATGCAGCG CGCGAACTCG CCCAGCGGCC GAGCTCGTCG GCGAGTGATC CGCAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT