MyabA.10520.a

putative beta-lactamase

CENTER ID: MyabA.10520.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.10520.a.A1.GE30543 Full length( MyabA.10520.a ) 1 223
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.10520.a.A1.PW30420 Full length( MyabA.10520.a ) 1 223
MyabA.10520.a.A1.PW31348 Full length( MyabA.10520.a ) 1 223
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127147
PATRIC ID: fig|36809.5.peg.2921
RefSeq: YP_001703606.1
UniProt: B1MCI1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLITGFPAGM FATNCYIVAP HEGGPAVIVD PGQDAIGGVK EILAERDLTP EAVLLTHGHL DHTWTAQPLA DEYGIPVYIH PDDRVMLADP LAGIGPGLGQ FIQGQVFAEP KELVEIGDGD KLELAGITLT VDHTPGHTRG SVVFGIEVDS ENGPVDVLFS GDTLFQMSIG RTDLPGGNHQ QLLDSIAAKL LTRDDSSVVL PGHGNATSIG DERRANPFLE GIQ
NT Sequence
GTGCTGATCA CCGGTTTTCC CGCGGGGATG TTCGCGACGA ACTGCTATAT CGTGGCGCCG CACGAGGGCG GCCCGGCCGT CATCGTCGAC CCCGGGCAGG ACGCCATCGG GGGCGTCAAG GAGATTCTCG CCGAGCGTGA TCTCACCCCG GAAGCGGTGT TGCTGACGCA CGGGCATCTG GATCACACCT GGACAGCGCA GCCTCTTGCC GATGAATATG GGATTCCCGT GTACATCCAT CCCGATGACC GCGTGATGCT CGCCGATCCG CTGGCCGGGA TCGGTCCGGG GCTGGGCCAG TTCATTCAGG GCCAGGTGTT CGCCGAGCCC AAGGAGTTGG TCGAGATCGG GGACGGCGAC AAATTGGAGC TCGCCGGGAT CACGCTCACC GTCGACCACA CCCCGGGGCA CACCCGCGGT TCTGTCGTGT TCGGTATCGA GGTTGATAGC GAGAACGGTC CCGTCGACGT GCTTTTCAGT GGTGACACCC TGTTCCAGAT GTCGATTGGG CGCACCGATC TACCCGGCGG AAACCACCAG CAGCTGCTCG ATTCGATCGC CGCCAAGCTG CTTACGCGCG ATGACTCCAG CGTTGTGCTG CCGGGACATG GCAATGCCAC CAGCATCGGC GACGAGCGCC GCGCCAACCC ATTCCTCGAG GGAATCCAG
Details for MyabA.10520.a.A1.GE30543
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVLITGFPA GMFATNCYIV APHEGGPAVI VDPGQDAIGG VKEILAERDL TPEAVLLTHG HLDHTWTAQP LADEYGIPVY IHPDDRVMLA DPLAGIGPGL GQFIQGQVFA EPKELVEIGD GDKLELAGIT LTVDHTPGHT RGSVVFGIEV DSENGPVDVL FSGDTLFQMS IGRTDLPGGN HQQLLDSIAA KLLTRDDSSV VLPGHGNATS IGDERRANPF LEGIQ
Validated NT Sequence
ttattactgg attccctcga ggaatgggtt ggcgcggcgc tcgtcgccga tgctggtggc attgccatgt cccggcagca caacgctgga gtcatcgcgc gtaagcagct tggcggcgat cgaatcgagc agctgctggt ggtttccgcc gggtagatcg gtgcgcccaa tcgacatctg gaacagggtg tcaccactga aaagcacgtc gacgggaccg ttctcgctat caacctcgat accgaacacg acagaaccgc gggtgtgccc cggggtgtgg tcgacggtga gcgtgatccc ggcgagctcc aatttgtcgc cgtccccgat ctcgaccaac tccttgggct cggcgaacac ctggccctga atgaactggc ccagccccgg accgatcccg gccagcggat cggcgagcat cacgcggtca tcgggatgga tgtacacggg aatcccatat tcatcggcaa gaggctgcgc tgtccaggtg tgatccagat gcccgtgcgt cagcaacacc gcttccgggg tgagatcacg ctcggcgaga atctccttga cgcccccgat ggcgtcctgc ccggggtcga cgatgacggc cgggccgccc tcgtgcggcg ccacgatata gcagttcgtc gcgaacatcc ccgcgggaaa accggtgatc agcaccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLITGFPA GMFATNCYIV APHEGGPAVI VDPGQDAIGG VKEILAERDL TPEAVLLTHG HLDHTWTAQP LADEYGIPVY IHPDDRVMLA DPLAGIGPGL GQFIQGQVFA EPKELVEIGD GDKLELAGIT LTVDHTPGHT RGSVVFGIEV DSENGPVDVL FSGDTLFQMS IGRTDLPGGN HQQLLDSIAA KLLTRDDSSV VLPGHGNATS IGDERRANPF LEGIQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgctgatc accggttttc ccgcggggat gttcgcgacg aactgctata tcgtggcgcc gcacgagggc ggcccggccg tcatcgtcga ccccgggcag gacgccatcg ggggcgtcaa ggagattctc gccgagcgtg atctcacccc ggaagcggtg ttgctgacgc acgggcatct ggatcacacc tggacagcgc agcctcttgc cgatgaatat gggattcccg tgtacatcca tcccgatgac cgcgtgatgc tcgccgatcc gctggccggg atcggtccgg ggctgggcca gttcattcag ggccaggtgt tcgccgagcc caaggagttg gtcgagatcg gggacggcga caaattggag ctcgccggga tcacgctcac cgtcgaccac accccggggc acacccgcgg ttctgtcgtg ttcggtatcg aggttgatag cgagaacggt cccgtcgacg tgcttttcag tggtgacacc ctgttccaga tgtcgattgg gcgcaccgat ctacccggcg gaaaccacca gcagctgctc gattcgatcg ccgccaagct gcttacgcgc gatgactcca gcgttgtgct gccgggacat ggcaatgcca ccagcatcgg cgacgagcgc cgcgccaacc cattcctcga gggaatccag taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MyabA.10520.a.A1.PW30420
PURIFICATION DATe: 12/13/2010
CONCENTRATION: 20.39mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVLITGFPA GMFATNCYIV APHEGGPAVI VDPGQDAIGG VKEILAERDL TPEAVLLTHG HLDHTWTAQP LADEYGIPVY IHPDDRVMLA DPLAGIGPGL GQFIQGQVFA EPKELVEIGD GDKLELAGIT LTVDHTPGHT RGSVVFGIEV DSENGPVDVL FSGDTLFQMS IGRTDLPGGN HQQLLDSIAA KLLTRDDSSV VLPGHGNATS IGDERRANPF LEGIQ
Validated NT Sequence
ttattactgg attccctcga ggaatgggtt ggcgcggcgc tcgtcgccga tgctggtggc attgccatgt cccggcagca caacgctgga gtcatcgcgc gtaagcagct tggcggcgat cgaatcgagc agctgctggt ggtttccgcc gggtagatcg gtgcgcccaa tcgacatctg gaacagggtg tcaccactga aaagcacgtc gacgggaccg ttctcgctat caacctcgat accgaacacg acagaaccgc gggtgtgccc cggggtgtgg tcgacggtga gcgtgatccc ggcgagctcc aatttgtcgc cgtccccgat ctcgaccaac tccttgggct cggcgaacac ctggccctga atgaactggc ccagccccgg accgatcccg gccagcggat cggcgagcat cacgcggtca tcgggatgga tgtacacggg aatcccatat tcatcggcaa gaggctgcgc tgtccaggtg tgatccagat gcccgtgcgt cagcaacacc gcttccgggg tgagatcacg ctcggcgaga atctccttga cgcccccgat ggcgtcctgc ccggggtcga cgatgacggc cgggccgccc tcgtgcggcg ccacgatata gcagttcgtc gcgaacatcc ccgcgggaaa accggtgatc agcaccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLITGFPA GMFATNCYIV APHEGGPAVI VDPGQDAIGG VKEILAERDL TPEAVLLTHG HLDHTWTAQP LADEYGIPVY IHPDDRVMLA DPLAGIGPGL GQFIQGQVFA EPKELVEIGD GDKLELAGIT LTVDHTPGHT RGSVVFGIEV DSENGPVDVL FSGDTLFQMS IGRTDLPGGN HQQLLDSIAA KLLTRDDSSV VLPGHGNATS IGDERRANPF LEGIQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCTGATC ACCGGTTTTC CCGCGGGGAT GTTCGCGACG AACTGCTATA TCGTGGCGCC GCACGAGGGC GGCCCGGCCG TCATCGTCGA CCCCGGGCAG GACGCCATCG GGGGCGTCAA GGAGATTCTC GCCGAGCGTG ATCTCACCCC GGAAGCGGTG TTGCTGACGC ACGGGCATCT GGATCACACC TGGACAGCGC AGCCTCTTGC CGATGAATAT GGGATTCCCG TGTACATCCA TCCCGATGAC CGCGTGATGC TCGCCGATCC GCTGGCCGGG ATCGGTCCGG GGCTGGGCCA GTTCATTCAG GGCCAGGTGT TCGCCGAGCC CAAGGAGTTG GTCGAGATCG GGGACGGCGA CAAATTGGAG CTCGCCGGGA TCACGCTCAC CGTCGACCAC ACCCCGGGGC ACACCCGCGG TTCTGTCGTG TTCGGTATCG AGGTTGATAG CGAGAACGGT CCCGTCGACG TGCTTTTCAG TGGTGACACC CTGTTCCAGA TGTCGATTGG GCGCACCGAT CTACCCGGCG GAAACCACCA GCAGCTGCTC GATTCGATCG CCGCCAAGCT GCTTACGCGC GATGACTCCA GCGTTGTGCT GCCGGGACAT GGCAATGCCA CCAGCATCGG CGACGAGCGC CGCGCCAACC CATTCCTCGA GGGAATCCAG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for MyabA.10520.a.A1.PW31348
PURIFICATION DATe: 12/13/2010
CONCENTRATION: 24.67mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVLITGFPA GMFATNCYIV APHEGGPAVI VDPGQDAIGG VKEILAERDL TPEAVLLTHG HLDHTWTAQP LADEYGIPVY IHPDDRVMLA DPLAGIGPGL GQFIQGQVFA EPKELVEIGD GDKLELAGIT LTVDHTPGHT RGSVVFGIEV DSENGPVDVL FSGDTLFQMS IGRTDLPGGN HQQLLDSIAA KLLTRDDSSV VLPGHGNATS IGDERRANPF LEGIQ
Validated NT Sequence
ttattactgg attccctcga ggaatgggtt ggcgcggcgc tcgtcgccga tgctggtggc attgccatgt cccggcagca caacgctgga gtcatcgcgc gtaagcagct tggcggcgat cgaatcgagc agctgctggt ggtttccgcc gggtagatcg gtgcgcccaa tcgacatctg gaacagggtg tcaccactga aaagcacgtc gacgggaccg ttctcgctat caacctcgat accgaacacg acagaaccgc gggtgtgccc cggggtgtgg tcgacggtga gcgtgatccc ggcgagctcc aatttgtcgc cgtccccgat ctcgaccaac tccttgggct cggcgaacac ctggccctga atgaactggc ccagccccgg accgatcccg gccagcggat cggcgagcat cacgcggtca tcgggatgga tgtacacggg aatcccatat tcatcggcaa gaggctgcgc tgtccaggtg tgatccagat gcccgtgcgt cagcaacacc gcttccgggg tgagatcacg ctcggcgaga atctccttga cgcccccgat ggcgtcctgc ccggggtcga cgatgacggc cgggccgccc tcgtgcggcg ccacgatata gcagttcgtc gcgaacatcc ccgcgggaaa accggtgatc agcaccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLITGFPA GMFATNCYIV APHEGGPAVI VDPGQDAIGG VKEILAERDL TPEAVLLTHG HLDHTWTAQP LADEYGIPVY IHPDDRVMLA DPLAGIGPGL GQFIQGQVFA EPKELVEIGD GDKLELAGIT LTVDHTPGHT RGSVVFGIEV DSENGPVDVL FSGDTLFQMS IGRTDLPGGN HQQLLDSIAA KLLTRDDSSV VLPGHGNATS IGDERRANPF LEGIQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCTGATC ACCGGTTTTC CCGCGGGGAT GTTCGCGACG AACTGCTATA TCGTGGCGCC GCACGAGGGC GGCCCGGCCG TCATCGTCGA CCCCGGGCAG GACGCCATCG GGGGCGTCAA GGAGATTCTC GCCGAGCGTG ATCTCACCCC GGAAGCGGTG TTGCTGACGC ACGGGCATCT GGATCACACC TGGACAGCGC AGCCTCTTGC CGATGAATAT GGGATTCCCG TGTACATCCA TCCCGATGAC CGCGTGATGC TCGCCGATCC GCTGGCCGGG ATCGGTCCGG GGCTGGGCCA GTTCATTCAG GGCCAGGTGT TCGCCGAGCC CAAGGAGTTG GTCGAGATCG GGGACGGCGA CAAATTGGAG CTCGCCGGGA TCACGCTCAC CGTCGACCAC ACCCCGGGGC ACACCCGCGG TTCTGTCGTG TTCGGTATCG AGGTTGATAG CGAGAACGGT CCCGTCGACG TGCTTTTCAG TGGTGACACC CTGTTCCAGA TGTCGATTGG GCGCACCGAT CTACCCGGCG GAAACCACCA GCAGCTGCTC GATTCGATCG CCGCCAAGCT GCTTACGCGC GATGACTCCA GCGTTGTGCT GCCGGGACAT GGCAATGCCA CCAGCATCGG CGACGAGCGC CGCGCCAACC CATTCCTCGA GGGAATCCAG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA