SSGCID: Target Page

MyabA.10014.a

Putative nitrate ABC transporter, ATP-binding protein (Rv1747 ortholog)

CENTER ID:	MyabA.10014.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.10014.a.B1.GE37301	full length		1	248

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.2284
RefSeq:	YP_001702978.1
UniProt:	B1MAQ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTRASALVLD EVRLGYGGRT VVDRLSLTVQ PGEILVLTGP SGCGKSTVLR ALAGLLKPDA GTIRADGETV VTTSRDRAMV FQDNALLPWR TVRSNIELAL KLGGIARQGR REAAQSWIDD VGLTGFENYL PKSLSGGMRQ RVQLARGLAG APRAVMMDEP FGALDAQTRA SMQRLLIDTW RSHPTTVVFV THDVDEALLL GDRIVVLGRA GQPLRALLDV PEPREADIDR SGLRTEILAA LNHTELVA

NT Sequence

atgacacgcg cttccgctct ggttctcgac gaggtcaggc tcggctacgg cggacgtacc gtcgtggacc gcctcagtct cacggtgcaa cctggggaaa tactggttct gaccggccct tcgggctgcg gcaagtccac tgtgctgcgc gccctggcgg ggctgctcaa gcccgatgcg ggaaccatcc gcgccgacgg cgagacggtg gtcaccacct cgcgcgaccg agccatggtc ttccaagata acgctttgtt gccctggcgc acagtgcgat ccaacataga actggcgttg aagctgggtg gtatcgctcg tcaggggcgg cgcgaggcgg cgcagtcctg gatcgacgat gtgggcctga ccgggttcga gaactatctg cccaagagcc tctcgggtgg catgcgccag cgtgtccagc tcgcccgcgg tctcgcggga gctccacgtg cggtcatgat ggacgagcca ttcggggcgc tggatgccca gacccgtgcc agcatgcagc gtctgctcat cgacacctgg cgcagtcatc ccaccacggt ggtgttcgtg actcacgatg tcgatgaggc gctgctactg ggggatcgca ttgtcgtcct gggcagggcg gggcagccat tgcgggcact cttggacgtg cccgaacctc gtgaggccga tatcgaccgc agtggtctac gcactgaaat ccttgccgca ctgaatcata cggagcttgt tgcctaa

Details for MyabA.10014.a.B1.GE37301

HARVESTED ON:	8/28/2013
SEQUENCED ON:	9/3/2013
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	95
PERCENT COVERAGE:	76

Validated AA Sequence

MAHHHHHHMT RASALVLDEV RLGYGGRTVV DRLSLTVQPG EILVLTGPSG CGKSTVLRAL AGLLKPDAGT IRADGETVVT TSRDRAMVFQ DNALLPWRTV RSNIELALKL GGIARQGRRE AAQSWIDDVG LTGFENYLPK SLSGGMRQRV QLARGLAGAP RAVMMDEPFG ALDAQTRASM QRLLXTPGAV IPPRWCS

Validated NT Sequence

cnnnnnnnat tttnnttact ttaagaagga ganataccat ggctcaccac caccaccacc atatgacacg cgcttccgct ctggttctcg acgaggtcag gctcggctac ggcggacgta ccgtcgtgga ccgcctcagt ctcacggtgc aacctgggga aatactggtt ctgaccggcc cttcgggctg cggcaagtcc actgtgctgc gcgccctggc ggggctgctc aagcccgatg cgggaaccat ccgcgccgac ggcgagacgg tggtcaccac ctcgcgcgac cgagccatgg tcttccaaga taacgctttg ttgccctggc gcacagtgcg atccaacata gaactggcgt tgaagctggg tggtatcgct cgtcaggggc ggcgcgaggc ggcgcagtcc tggatcgacg atgtgggcct gaccgggttc gagaactatc tgcccaagag cctctcgggt ggcatgcgcc agcgtgtcca gctcgcccgc ggtctcgcgg gagctccacg tgcggtcatg atggacgagc cattcggggc gctggatgcc cagacccgtg ccagcatgca gcgtctgctn ncgacacctg gcgcagtcat cccaccacgg tggtgttcgt gactcacgat gtcgatgagg cgctgctact gggggatcgc attgtcgtcc tgggcagggc ggggcagcca ttgcgggcac tcttggacgt gcccgaacct cgtgaggncn atatcgaccg cagtgntcta cgcactgaaa tccttgccgc actgaatcat acggagcttg ttgcctaant gagtaanata ggatccngnt nnnnnnnccc ccnnnnnnnn nnnnnaaann aaannnnnan nnnnnnnnan ngnnngnnnn ncnnnnngnn nnnnnntnnn ggnnnnnagt atnnntnnan ggnnnnnnnn nnnntcnngn nnnnnnnnnn nnnnnnnnnn nngggnntnn gtcnnnnnna nntnnnnnnn ngnnnnntnn nnnnnnnacn nnnntnnnnn cgnnngnnnn nnnnntntat nnnnnacnnn nngnnangtt nnnngtnnnn nntgnnannn nnnnngnnng gg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac gcgcttccgc tctggttctc gacgaggtca ggctcggcta cggcggacgt accgtcgtgg accgcctcag tctcacggtg caacctgggg aaatactggt tctgaccggc ccttcgggct gcggcaagtc cactgtgctg cgcgccctgg cggggctgct caagcccgat gcgggaacca tccgcgccga cggcgagacg gtggtcacca cctcgcgcga ccgagccatg gtcttccaag ataacgcttt gttgccctgg cgcacagtgc gatccaacat agaactggcg ttgaagctgg gtggtatcgc tcgtcagggg cggcgcgagg cggcgcagtc ctggatcgac gatgtgggcc tgaccgggtt cgagaactat ctgcccaaga gcctctcggg tggcatgcgc cagcgtgtcc agctcgcccg cggtctcgcg ggagctccac gtgcggtcat gatggacgag ccattcgggg cgctggatgc ccagacccgt gccagcatgc agcgtctgct catcgacacc tggcgcagtc atcccaccac ggtggtgttc gtgactcacg atgtcgatga ggcgctgcta ctgggggatc gcattgtcgt cctgggcagg gcggggcagc cattgcgggc actcttggac gtgcccgaac ctcgtgaggc cgatatcgac cgcagtggtc tacgcactga aatccttgcc gcactgaatc atacggagct tgttgcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at