MyabA.01613.c

Probable acyl-CoA dehydrogenase FadE

CENTER ID: MyabA.01613.c
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01613.c.A1.GE30533 Full length( MyabA.01613.c ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.635
RefSeq: YP_001701347.1
UniProt: B1MH09

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLLLDDETE AFRAEVREFL VSNVPNPPLP SYDSPEGAAA HRQWERTLYD AGFSAVSWPK EYGGRDVTPL QWVIFEEEYF KAGAPARISQ NGMFLLGPTL FAHGSKDQLD RVLPKMANGE EIWAQAWSEP ESGSDLASLR SPARKVDGGW RLSGQKIWSS RAPFADRAFG LFRSDPDAQR HQGLTYFMFD LKAEGITVRP IPQLDGDPGF AEIFLDDVFV PDEDVIGAVH DGWRVAMSTS SNERGMSLRS PGRFLATADR LVELWKSVPE NEFASERVAD AWIAAQAYRL HTFGTVTRLA GGGELGAESS ITKVFWSELD VALHETAIDL RGAEGELTNA WTDGYLFSLG GPIYAGTNEI QRNIIAERIL GLPRESSASA RTGGAK
NT Sequence
GTGGACCTAC TTCTTGACGA TGAGACCGAG GCGTTCCGGG CCGAGGTCCG CGAGTTCCTC GTATCCAATG TCCCCAATCC GCCGCTGCCG TCCTATGACT CACCGGAAGG TGCGGCGGCA CACCGGCAAT GGGAACGCAC CCTGTACGAC GCGGGATTCT CCGCCGTGTC CTGGCCCAAG GAATACGGCG GCCGAGATGT CACGCCGCTG CAGTGGGTGA TCTTCGAAGA GGAATACTTC AAGGCCGGTG CGCCCGCGCG CATCAGCCAG AACGGCATGT TCTTGTTGGG GCCGACGCTG TTCGCCCACG GCAGCAAGGA CCAGCTGGAT CGCGTGCTTC CGAAGATGGC CAACGGGGAA GAGATCTGGG CACAGGCATG GTCCGAGCCC GAGTCCGGCA GCGACCTGGC GTCGCTGCGC TCCCCCGCTC GAAAGGTGGA CGGGGGCTGG CGTCTGTCTG GCCAGAAGAT CTGGAGTTCA CGAGCGCCCT TCGCGGACAG AGCATTCGGC TTGTTCCGTT CCGATCCGGA CGCGCAGCGC CATCAGGGTC TGACGTATTT CATGTTTGAT CTGAAGGCGG AGGGCATCAC CGTGCGCCCG ATCCCGCAGC TCGACGGTGA TCCCGGGTTC GCGGAAATCT TCCTGGACGA CGTGTTTGTG CCCGATGAAG ACGTCATCGG CGCGGTGCAT GACGGCTGGC GCGTGGCGAT GAGCACCTCG AGCAACGAGC GTGGCATGTC GCTACGCAGC CCCGGACGCT TCCTGGCCAC CGCAGACCGC CTGGTCGAGC TGTGGAAATC GGTTCCGGAG AACGAGTTCG CATCCGAACG CGTTGCCGAC GCCTGGATCG CGGCGCAGGC ATACCGGCTG CACACATTTG GCACCGTGAC CCGGCTGGCC GGTGGCGGGG AGCTTGGCGC CGAATCCTCG ATCACCAAGG TGTTCTGGTC CGAGCTCGAT GTCGCCTTGC ACGAGACCGC AATCGACCTG CGCGGGGCCG AGGGCGAGTT GACGAATGCT TGGACGGACG GATACCTGTT CTCGCTCGGC GGTCCGATTT ATGCGGGCAC CAACGAGATT CAGCGCAATA TCATCGCCGA GCGCATCCTG GGCCTACCCA GAGAAAGCAG CGCAAGCGCT CGCACGGGAG GTGCCAAG
Details for MyabA.01613.c.A1.GE30533
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDLLLDDE TEAFRAEVRE FLVSNVPNPP LPSYDSPEGA AAHRQWERTL YDAGFSAVSW PKEYGGRDVT PLQWVIFEEE YFKAGAPARI SQNGMFLLGP TLFAHGSKDQ LDRVLPKMAN GEEIWAQAWS EPESGSDLAS LRSPARKVDG GWRLSGQKIW SSRAPFADRA FGLFRSDPDA QRHQGLTYFM FDLKAEGITV RPIPQLDGDP GFAEIFLDDV FVPDEDVIGA VHDGWRVAMS TSSNERGMSL RSPGXFLATA DRLVXLWKSV PENEFASERV AXAWIAAXXT GCTHLAXXXX XPXXGXXXXX LXXXXXXXXM SXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
ttgtttactt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtggacc tacttcttga cgatgagacc gaggcgttcc gggccgaggt ccgcgagttc ctcgtatcca atgtccccaa tccgccgctg ccgtcctatg actcaccgga aggtgcggcg gcacaccggc aatgggaacg caccctgtac gacgcgggat tctccgccgt gtcctggccc aaggaatacg gcggccgaga tgtcacgccg ctgcagtggg tgatcttcga agaggaatac ttcaaggccg gtgcgcccgc gcgcatcagc cagaacggca tgttcttgtt ggggccgacg ctgttcgccc acggcagcaa ggaccagctg gatcgcgtgc ttccgaagat ggccaacggg gaagagatct gggcacaggc atggtccgag cccgagtccg gcagcgacct ggcgtcgctg cgctcccccg ctcgaaaggt ggacgggggc tggcgtctgt ctggccagaa gatctggagt tcacgagcgc ccttcgcgga cagagcattc ggcttgttcc gttccgatcc ggacgcgcag cgccatcagg gtctgacgta tttcatgttt gatctgaagg cggagggcat caccgtgcgc ccgatcccgc agctcgacgg tgatcccggg ttcgcggaaa tcttcctgga cgacgtgttt gtgcccgatg aagacgtcat cggcgcggtg catgacggct ggcgcgtggc gatgagcacc tcgagcaacg agcgtggcat gtcgctacgc agccccggan gcttcctggc caccgcagac cgcctggtcg anctgtggaa atcggttccn gagaacgagt tcgcatccga acgcgttgcc nacgcctgga tcgcggcgcn nnataccggc tgcacacatt tggcanngng acnnnncngn ccnnngnngg gnnnctnngn nncnaanctc natcancnnn nnnngncnan ctnnatgtcn nnnncncnan annnnannna cnnnnngngn ncnangncnn nnngannann nnngnnngnn nnnnncnnnn ncnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcnnnnn nnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDLLLDDE TEAFRAEVRE FLVSNVPNPP LPSYDSPEGA AAHRQWERTL YDAGFSAVSW PKEYGGRDVT PLQWVIFEEE YFKAGAPARI SQNGMFLLGP TLFAHGSKDQ LDRVLPKMAN GEEIWAQAWS EPESGSDLAS LRSPARKVDG GWRLSGQKIW SSRAPFADRA FGLFRSDPDA QRHQGLTYFM FDLKAEGITV RPIPQLDGDP GFAEIFLDDV FVPDEDVIGA VHDGWRVAMS TSSNERGMSL RSPGRFLATA DRLVELWKSV PENEFASERV ADAWIAAQAY RLHTFGTVTR LAGGGELGAE SSITKVFWSE LDVALHETAI DLRGAEGELT NAWTDGYLFS LGGPIYAGTN EIQRNIIAER ILGLPRESSA SARTGGAK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggaccta cttcttgacg atgagaccga ggcgttccgg gccgaggtcc gcgagttcct cgtatccaat gtccccaatc cgccgctgcc gtcctatgac tcaccggaag gtgcggcggc acaccggcaa tgggaacgca ccctgtacga cgcgggattc tccgccgtgt cctggcccaa ggaatacggc ggccgagatg tcacgccgct gcagtgggtg atcttcgaag aggaatactt caaggccggt gcgcccgcgc gcatcagcca gaacggcatg ttcttgttgg ggccgacgct gttcgcccac ggcagcaagg accagctgga tcgcgtgctt ccgaagatgg ccaacgggga agagatctgg gcacaggcat ggtccgagcc cgagtccggc agcgacctgg cgtcgctgcg ctcccccgct cgaaaggtgg acgggggctg gcgtctgtct ggccagaaga tctggagttc acgagcgccc ttcgcggaca gagcattcgg cttgttccgt tccgatccgg acgcgcagcg ccatcagggt ctgacgtatt tcatgtttga tctgaaggcg gagggcatca ccgtgcgccc gatcccgcag ctcgacggtg atcccgggtt cgcggaaatc ttcctggacg acgtgtttgt gcccgatgaa gacgtcatcg gcgcggtgca tgacggctgg cgcgtggcga tgagcacctc gagcaacgag cgtggcatgt cgctacgcag ccccggacgc ttcctggcca ccgcagaccg cctggtcgag ctgtggaaat cggttccgga gaacgagttc gcatccgaac gcgttgccga cgcctggatc gcggcgcagg cataccggct gcacacattt ggcaccgtga cccggctggc cggtggcggg gagcttggcg ccgaatcctc gatcaccaag gtgttctggt ccgagctcga tgtcgccttg cacgagaccg caatcgacct gcgcggggcc gagggcgagt tgacgaatgc ttggacggac ggatacctgt tctcgctcgg cggtccgatt tatgcgggca ccaacgagat tcagcgcaat atcatcgccg agcgcatcct gggcctaccc agagaaagca gcgcaagcgc tcgcacggga ggtgccaagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa