MyabA.01613.b

Putative acyl-CoA dehydrogenase

CENTER ID: MyabA.01613.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01613.b.A1.GE30532 Full length( MyabA.01613.b ) 1 413
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.3174
RefSeq: YP_001703853.1
UniProt: B1MD78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPVTSSEPSS AGSNALHTSG SSQRDRVAAA AHEVLAKHPP ATTPVTDLLG AVYDAGLAWV HFPVGLGGLD APPALQAVSD TILRAAGVPD PLFVNVIGYG MAAPTVLAHG QPELIRRILR PLFTGEELWC QLFSEPGAGS DLAGLATRAV RDGDEWVING QKVWTSGAHQ ARWALLVARS NPDVAKHRGL TYFVVDMTDP GVEVRPLRQM TGDAEFNEVY LTDVRIPDAH RLGNEGDGWR VSMTTLMNER SALGGAFDHG RGGGSIGNAL NLWKQRPDLH TPELRAKLTN LFVRSEGNRY GTQMRIAAQG DAPMGPEGSI GKLMGAELNQ QIYDFCVELL GIEATLYASY DMRRVVEDDR RADTMWAFLR SKANTIEGGT SDVMRNIIGE RVLGLPGDIR VDTDKAWKDV PRG
NT Sequence
ATGCCAGTGA CGTCATCGGA GCCGTCTAGC GCCGGGTCCA ACGCCCTTCA TACCAGCGGT TCATCGCAAC GCGACCGTGT CGCCGCTGCC GCCCACGAGG TGCTCGCCAA ACATCCCCCC GCGACGACGC CGGTGACCGA CCTATTGGGC GCCGTCTACG ACGCGGGCCT GGCCTGGGTA CATTTCCCGG TCGGCCTGGG TGGCCTGGAC GCGCCGCCGG CGCTGCAGGC CGTTTCCGAC ACCATCCTGC GCGCGGCCGG TGTGCCAGAC CCCTTGTTTG TCAACGTGAT CGGGTATGGC ATGGCAGCGC CGACGGTACT AGCGCATGGA CAACCGGAAT TGATCCGGCG GATCCTGCGT CCCCTGTTCA CCGGTGAAGA GCTGTGGTGT CAGCTCTTCA GCGAGCCGGG TGCCGGCTCC GATTTGGCAG GCCTGGCCAC CCGCGCGGTA CGCGACGGCG ATGAGTGGGT GATCAACGGT CAGAAGGTGT GGACCTCGGG GGCGCATCAG GCGCGCTGGG CCTTGTTGGT GGCCCGCAGC AATCCCGACG TGGCCAAGCA TCGGGGCCTG ACCTACTTCG TCGTCGACAT GACCGATCCC GGCGTCGAGG TCCGTCCCCT GCGCCAGATG ACCGGCGACG CCGAGTTCAA CGAGGTCTAT CTCACCGATG TCCGCATCCC GGACGCACAC CGCCTGGGCA ACGAGGGCGA TGGCTGGCGG GTGTCGATGA CCACGTTGAT GAACGAGCGC AGCGCACTGG GTGGCGCCTT CGATCACGGC CGCGGCGGTG GCTCGATCGG CAATGCCCTC AATCTGTGGA AGCAGCGCCC CGATCTGCAC ACTCCCGAGC TGCGGGCCAA GCTGACGAAT CTTTTCGTAC GCTCGGAAGG CAACCGCTAC GGCACACAGA TGAGGATCGC CGCCCAGGGC GATGCGCCTA TGGGTCCCGA AGGATCTATC GGCAAGCTGA TGGGCGCGGA ACTCAACCAG CAGATCTACG ACTTCTGTGT TGAACTGCTG GGCATCGAGG CGACCCTGTA CGCCTCCTAT GACATGCGCC GGGTGGTGGA AGACGACCGG CGTGCCGACA CCATGTGGGC CTTCCTGCGA TCCAAGGCCA ACACCATCGA AGGCGGTACC TCCGACGTGA TGCGCAACAT CATCGGAGAA CGCGTACTCG GATTGCCCGG CGATATTCGG GTCGACACCG ACAAAGCCTG GAAGGACGTG CCCCGCGGA
Details for MyabA.01613.b.A1.GE30532
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 46kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 89
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPVTSSEPS SAGSNALHTS GSSQRDRVAA AAHEVLAKHP PATTPVTDLL GAVYDAGLAW VHFPVGLGGL DAPPALQAVS DTILRAAGVP DPLFVNVIGY GMAAPTVLAH GQPELIRRIL RPLFTGEELW CQLFSEPGAG SDLAGLATRA VRDGDEWVIN GQKVWTSGAH QARWALLVAR SNPDVAKHRG LTYFVVDMTD PGVEVRPLRQ MTGDAXFNEV YLTDVRIPDA HRLXNEGDGW RVSMTTLMNE RSALGGAFDH GRGGGSIGNA LNLWKQRPDL HTPELRXKLX NLFVXSXGXX YGTQXXDRRP XXAPXGXXXX XGXLMGXEXX SXXXXXXXXX XXXXXTXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
attttgttta ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccag tgacgtcatc ggagccgtct agcgccgggt ccaacgccct tcataccagc ggttcatcgc aacgcgaccg tgtcgccgct gccgcccacg aggtgctcgc caaacatccc cccgcgacga cgccggtgac cgacctattg ggcgccgtct acgacgcggg cctggcctgg gtacatttcc cggtcggcct gggtggcctg gacgcgccgc cggcgctgca ggccgtttcc gacaccatcc tgcgcgcggc cggtgtgcca gaccccttgt ttgtcaacgt gatcgggtat ggcatggcag cgccgacggt actagcgcat ggacaaccgg aattgatccg gcggatcctg cgtcccctgt tcaccggtga agagctgtgg tgtcagctct tcagcgagcc gggtgccggc tccgatttgg caggcctggc cacccgcgcg gtacgcgacg gcgatgagtg ggtgatcaac ggtcagaagg tgtggacctc gggggcgcat caggcgcgct gggccttgtt ggtggcccgc agcaatcccg acgtggccaa gcatcggggc ctgacctact tcgtcgtcga catgaccgat cccggcgtcg aggtccgtcc cctgcgccag atgaccggcg acgccgantt caacgaggtc tatctcaccg atgtccgcat cccggacgca caccgcctgg ncaacgaggg cgatggctgg cgggtgtcga tgaccacgtt gatgaacgag cgcagcgcac tgggtggcgc cttcgatcac ggccgcggcg gtggctcgat cggcaatgcc ctcaatctgt ggaagcagcg ccccgatctg cacactcccg agctgcgggn caagctgang aatcttttcg tangctcngn nggnnncngc tacggcacnc agntgangga tcgccgnccn nnnnatgcnc ctnntgggnn ccnnnnnnct ancggcnnnc tnatgggcnn ngaantnnna tctncnantn nnnnnntnan ngnnnnnnnt nnnnnnnnan cnnnnacgnc nnnnntnnnn nnnnnnnnnn nnnnnnnann annnnnnnnn ncnnnnnnnn nnnnnnnnnn nnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPVTSSEPS SAGSNALHTS GSSQRDRVAA AAHEVLAKHP PATTPVTDLL GAVYDAGLAW VHFPVGLGGL DAPPALQAVS DTILRAAGVP DPLFVNVIGY GMAAPTVLAH GQPELIRRIL RPLFTGEELW CQLFSEPGAG SDLAGLATRA VRDGDEWVIN GQKVWTSGAH QARWALLVAR SNPDVAKHRG LTYFVVDMTD PGVEVRPLRQ MTGDAEFNEV YLTDVRIPDA HRLGNEGDGW RVSMTTLMNE RSALGGAFDH GRGGGSIGNA LNLWKQRPDL HTPELRAKLT NLFVRSEGNR YGTQMRIAAQ GDAPMGPEGS IGKLMGAELN QQIYDFCVEL LGIEATLYAS YDMRRVVEDD RRADTMWAFL RSKANTIEGG TSDVMRNIIG ERVLGLPGDI RVDTDKAWKD VPRG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccagtgacg tcatcggagc cgtctagcgc cgggtccaac gcccttcata ccagcggttc atcgcaacgc gaccgtgtcg ccgctgccgc ccacgaggtg ctcgccaaac atccccccgc gacgacgccg gtgaccgacc tattgggcgc cgtctacgac gcgggcctgg cctgggtaca tttcccggtc ggcctgggtg gcctggacgc gccgccggcg ctgcaggccg tttccgacac catcctgcgc gcggccggtg tgccagaccc cttgtttgtc aacgtgatcg ggtatggcat ggcagcgccg acggtactag cgcatggaca accggaattg atccggcgga tcctgcgtcc cctgttcacc ggtgaagagc tgtggtgtca gctcttcagc gagccgggtg ccggctccga tttggcaggc ctggccaccc gcgcggtacg cgacggcgat gagtgggtga tcaacggtca gaaggtgtgg acctcggggg cgcatcaggc gcgctgggcc ttgttggtgg cccgcagcaa tcccgacgtg gccaagcatc ggggcctgac ctacttcgtc gtcgacatga ccgatcccgg cgtcgaggtc cgtcccctgc gccagatgac cggcgacgcc gagttcaacg aggtctatct caccgatgtc cgcatcccgg acgcacaccg cctgggcaac gagggcgatg gctggcgggt gtcgatgacc acgttgatga acgagcgcag cgcactgggt ggcgccttcg atcacggccg cggcggtggc tcgatcggca atgccctcaa tctgtggaag cagcgccccg atctgcacac tcccgagctg cgggccaagc tgacgaatct tttcgtacgc tcggaaggca accgctacgg cacacagatg aggatcgccg cccagggcga tgcgcctatg ggtcccgaag gatctatcgg caagctgatg ggcgcggaac tcaaccagca gatctacgac ttctgtgttg aactgctggg catcgaggcg accctgtacg cctcctatga catgcgccgg gtggtggaag acgaccggcg tgccgacacc atgtgggcct tcctgcgatc caaggccaac accatcgaag gcggtacctc cgacgtgatg cgcaacatca tcggagaacg cgtactcgga ttgcccggcg atattcgggt cgacaccgac aaagcctgga aggacgtgcc ccgcggataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa