MyabA.01613.a

Probable acyl-CoA dehydrogenase

CENTER ID: MyabA.01613.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01613.a.A1.GE30531 Full length( MyabA.01613.a ) 1 409
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.2537
RefSeq: YP_001703230.1
UniProt: B1MBF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLAFSAEEE QFRTEVREFL DANLTAELRA AGRLQTSVYS DHEASMRWQA ILHRKGWAAP AWPVKYGGQP WSLAQHYIFS VESMVAGAPS LSPMGIRMVA HAIIKFGSPE QKEFFLPRIL TGEVFFCQGY SEPESGSDLA SLQMSAVADG DDFVLNGSKI WTTHAREANW MFALVRTTKG PKKQLGITFI LIDFTSPGID VKPLVMSSGE EVQNVVFFDN VRVPRANVLG EVDQGWTVAK YLLEFERGGG AVAPMLQVGL DQLSVDARSV PAENGTLADD PVFCAKLADL AIRVDVLEVL EHRVLAVVAG GGNPGAASSM LKILGTELSQ ALTTLTLEAA GPRGRAYQPH VTKPGGPVVE YTPPADGYHS GELWQAVAPL HYFNDRAGSI YAGSNEIQRN ILAKAQLGL
NT Sequence
ATGGACCTGG CCTTCTCGGC AGAGGAAGAA CAATTCCGTA CCGAAGTCCG GGAGTTCCTG GACGCGAACC TCACCGCGGA GCTGCGGGCG GCCGGGCGTC TGCAGACCAG CGTGTACAGC GATCACGAGG CAAGTATGCG GTGGCAGGCG ATCCTGCACC GGAAGGGATG GGCCGCGCCG GCTTGGCCGG TCAAATACGG CGGGCAGCCG TGGAGTCTTG CCCAGCACTA CATCTTTTCG GTTGAATCGA TGGTTGCGGG GGCACCTTCA CTGTCGCCCA TGGGAATTCG GATGGTCGCA CATGCCATCA TCAAGTTCGG CAGCCCGGAA CAGAAAGAGT TCTTCCTGCC CCGGATCCTC ACGGGCGAGG TGTTCTTCTG TCAGGGGTAC AGCGAGCCCG AGTCGGGGTC TGACCTGGCC TCGTTGCAGA TGTCGGCCGT TGCCGACGGT GATGACTTCG TCCTCAATGG CTCCAAGATC TGGACCACCC ATGCGCGCGA GGCGAATTGG ATGTTCGCCC TTGTTCGCAC CACCAAGGGG CCCAAGAAAC AGCTCGGTAT CACCTTCATT CTCATCGACT TCACGTCGCC GGGCATAGAC GTCAAACCCT TGGTGATGAG TTCCGGCGAG GAGGTACAGA ATGTCGTGTT CTTCGACAAC GTGCGTGTGC CGCGGGCCAA TGTGCTCGGG GAGGTTGACC AGGGGTGGAC CGTCGCGAAG TACCTCCTTG AGTTCGAGCG CGGCGGCGGG GCCGTGGCAC CCATGCTGCA GGTGGGCCTC GATCAGCTGA GCGTTGACGC GCGGTCGGTG CCTGCCGAGA ACGGCACCCT GGCAGACGAT CCCGTGTTCT GTGCCAAACT GGCCGACCTG GCGATTCGTG TCGACGTACT CGAGGTCCTC GAGCATCGCG TGCTGGCGGT CGTGGCAGGC GGCGGCAATC CGGGCGCGGC ATCCTCGATG CTGAAAATCC TTGGCACCGA ACTATCGCAG GCGTTGACCA CACTGACGTT AGAGGCGGCA GGGCCGCGGG GCCGGGCATA CCAGCCTCAC GTCACCAAGC CGGGTGGCCC GGTCGTCGAG TACACCCCGC CCGCAGACGG TTATCACAGC GGCGAGCTGT GGCAGGCGGT GGCGCCGTTG CACTATTTCA ACGATCGCGC CGGGTCGATC TATGCCGGTT CCAACGAGAT TCAACGCAAC ATCCTCGCCA AGGCCCAGCT GGGTCTC
Details for MyabA.01613.a.A1.GE30531
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 46kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 88
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDLAFSAEE EQFRTEVREF LDANLTAELR AXGRLQTSVY SDHEASMRWQ AILHRKGWAA PAWPVKYGGQ PWSLAQHYIF SVESMVAGAP SLSPMGIRMV AHAIIKFGSP EQKEFFLPRI LTGEVFFCQG YSEPESGSDL ASLQMSAVAD GDDFVLNGSK IWTTHAREAN WMFALVRTTK GPKKQLGITF ILIDFTSPGI DVKPLVMSSG EEVQNVVFFD NVRVPRANVL GXVDQGWXVA KYLLEFERGG GAVAPMLXVG LDXLXVDARS XPAXXGTLXX DPXXCXXLXD LAIRXXVLXG PXASXCWXXX XXXXXXXXXX XXAXNXXXXX SXXTXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
attttgtnnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggacc tggccttctc ggcagaggaa gaacaattcc gtaccgaagt ccgggagttc ctggacgcga acctcaccgc ggagctgcgg gcggncgggc gtctgcagac cagcgtgtac agcgatcacg aggcaagtat gcggtggcag gcgatcctgc accggaaggg atgggccgcg ccggcttggc cggtcaaata cggcgggcag ccgtggagtc ttgcccagca ctacatcttt tcggttgaat cgatggttgc gggggcacct tcactgtcgc ccatgggaat tcggatggtc gcacatgcca tcatcaagtt cggcagcccg gaacagaaag agttcttcct gccccggatc ctcacgggcg aggtgttctt ctgtcagggg tacagcgagc ccgagtcggg gtctgacctg gcctcgttgc agatgtcggc cgttgccgac ggtgatgact tcgtcctcaa tggctccaag atctggacca cccatgcgcg cgaggcgaat tggatgttcg cccttgttcg caccaccaag gggcccaaga aacagctcgg tatcaccttc attctcatcg acttcacgtc gccgggcata gacgtcaaac ccttggtgat gagttccggc gaggaggtac agaatgtcgt gttcttcgac aacgtgcgtg tgccgcgggc caatgtgctc gggnaggttg accaggggtg gancgtcgcg aagtacctcc ttgagttcga gcgcggcggc ggggccgtgg cacccatgct gcangtgggc ctcgatcanc tgancgttga cgcgcggtcg gngcctgccg anaanggcac cctgncagan gatcccgnnn tctgtgnnna actgnccgac ctggcgattc gtgnnnacgt actcnanggt cctcnagcat cnnngtgctg gnggncnnnn nnnncngnng ncannncnng nnncngnntc cncnatgctn aaaatncnnn gnnncnnnnn tccngnnnna cnnnnnntnn nnnnnnnann gnnngnnnnn nnnnnnggcn nnnnnnncnn tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLAFSAEE EQFRTEVREF LDANLTAELR AAGRLQTSVY SDHEASMRWQ AILHRKGWAA PAWPVKYGGQ PWSLAQHYIF SVESMVAGAP SLSPMGIRMV AHAIIKFGSP EQKEFFLPRI LTGEVFFCQG YSEPESGSDL ASLQMSAVAD GDDFVLNGSK IWTTHAREAN WMFALVRTTK GPKKQLGITF ILIDFTSPGI DVKPLVMSSG EEVQNVVFFD NVRVPRANVL GEVDQGWTVA KYLLEFERGG GAVAPMLQVG LDQLSVDARS VPAENGTLAD DPVFCAKLAD LAIRVDVLEV LEHRVLAVVA GGGNPGAASS MLKILGTELS QALTTLTLEA AGPRGRAYQP HVTKPGGPVV EYTPPADGYH SGELWQAVAP LHYFNDRAGS IYAGSNEIQR NILAKAQLGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacctggcc ttctcggcag aggaagaaca attccgtacc gaagtccggg agttcctgga cgcgaacctc accgcggagc tgcgggcggc cgggcgtctg cagaccagcg tgtacagcga tcacgaggca agtatgcggt ggcaggcgat cctgcaccgg aagggatggg ccgcgccggc ttggccggtc aaatacggcg ggcagccgtg gagtcttgcc cagcactaca tcttttcggt tgaatcgatg gttgcggggg caccttcact gtcgcccatg ggaattcgga tggtcgcaca tgccatcatc aagttcggca gcccggaaca gaaagagttc ttcctgcccc ggatcctcac gggcgaggtg ttcttctgtc aggggtacag cgagcccgag tcggggtctg acctggcctc gttgcagatg tcggccgttg ccgacggtga tgacttcgtc ctcaatggct ccaagatctg gaccacccat gcgcgcgagg cgaattggat gttcgccctt gttcgcacca ccaaggggcc caagaaacag ctcggtatca ccttcattct catcgacttc acgtcgccgg gcatagacgt caaacccttg gtgatgagtt ccggcgagga ggtacagaat gtcgtgttct tcgacaacgt gcgtgtgccg cgggccaatg tgctcgggga ggttgaccag gggtggaccg tcgcgaagta cctccttgag ttcgagcgcg gcggcggggc cgtggcaccc atgctgcagg tgggcctcga tcagctgagc gttgacgcgc ggtcggtgcc tgccgagaac ggcaccctgg cagacgatcc cgtgttctgt gccaaactgg ccgacctggc gattcgtgtc gacgtactcg aggtcctcga gcatcgcgtg ctggcggtcg tggcaggcgg cggcaatccg ggcgcggcat cctcgatgct gaaaatcctt ggcaccgaac tatcgcaggc gttgaccaca ctgacgttag aggcggcagg gccgcggggc cgggcatacc agcctcacgt caccaagccg ggtggcccgg tcgtcgagta caccccgccc gcagacggtt atcacagcgg cgagctgtgg caggcggtgg cgccgttgca ctatttcaac gatcgcgccg ggtcgatcta tgccggttcc aacgagattc aacgcaacat cctcgccaag gcccagctgg gtctctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa