SSGCID: Target Page

MyabA.01582.a

Probable short-chain dehydrogenase/reductase

CENTER ID:	MyabA.01582.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01582.a.A1.GE30525	Full length( MyabA.01582.a )		1	292

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01582.a.A1.PW30402	Full length( MyabA.01582.a )		1	292

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.413
RefSeq:	YP_001701133.1
UniProt:	B1MFS9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGQLDGRVAV ITGAGRGIGR EHALLFAKEG ASVVVNDLGG ANDGSGSDAG PAQEVVDEIT AAGGKAVANT DNISTWAGAS NLVNQAVETF GQLDAVVNNA GILRDGFIAG LEEEQWDAVI QVHLKGHFAV LRHAAEYWKG QAKAGADIKA TVVNTASDSG VTLPNPGQGN YGAAKAGIAA LSCVAAAELE RYGVKVNAIA PVARTRLTLA TPGMGAIFAA PVEEGAFDMF SPANISPLVA YLSTEKNPWT GQVFKVTGGS IQRLKGWSVT DTAETDGPWS IDLVRDSIEP WK

NT Sequence

ATGGGACAAC TTGACGGGCG GGTGGCGGTC ATCACCGGTG CCGGGCGCGG CATCGGTCGT GAGCACGCCC TGCTCTTCGC CAAGGAAGGT GCTTCGGTGG TCGTCAACGA CCTCGGTGGC GCCAATGACG GCTCCGGCTC GGATGCCGGG CCCGCACAGG AAGTGGTGGA CGAGATCACC GCGGCGGGAG GCAAAGCCGT TGCGAATACC GACAACATCT CCACGTGGGC CGGCGCCTCG AACCTGGTGA ACCAGGCCGT CGAGACCTTC GGGCAGCTGG ATGCCGTGGT GAACAACGCG GGCATCCTCC GTGACGGTTT CATCGCCGGC CTTGAGGAAG AACAGTGGGA CGCGGTTATC CAGGTCCACC TCAAGGGGCA TTTCGCGGTG CTACGCCACG CCGCCGAATA CTGGAAGGGG CAGGCCAAGG CCGGCGCCGA TATCAAGGCC ACCGTGGTGA ACACGGCATC GGACTCCGGT GTGACGCTGC CGAACCCGGG CCAGGGCAAC TATGGTGCCG CCAAGGCGGG CATCGCGGCC CTGTCCTGTG TCGCGGCCGC CGAACTGGAG CGCTACGGCG TCAAGGTGAA TGCCATTGCG CCGGTTGCCC GCACCCGCCT CACGCTGGCG ACCCCCGGTA TGGGTGCCAT CTTCGCGGCT CCGGTGGAGG AGGGGGCGTT CGATATGTTC AGCCCCGCGA ACATATCCCC GCTGGTGGCA TACCTGAGTA CCGAGAAGAA CCCGTGGACC GGGCAGGTCT TCAAGGTCAC GGGCGGTTCG ATCCAGCGGC TCAAGGGCTG GTCGGTCACC GACACCGCCG AGACCGACGG GCCGTGGAGC ATCGACCTGG TGCGCGACAG CATCGAGCCC TGGAAG

Details for MyabA.01582.a.A1.GE30525

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	32kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMGQLDGR VAVITGAGRG IGREHALLFA KEGASVVVND LGGANDGSGS DAGPAQEVVD EITAAGGKAV ANTDNISTWA GASNLVNQAV ETFGQLDAVV NNAGILRDGF IAGLEEEQWD AVIQVHLKGH FAVLRHAAEY WKGQAKAGAD IKATVVNTAS DSGVTLPNPG QGNYGAAKAG IAALSCVAAA ELERYGVKVN AIAPVARTRL TLATPGMGAI FAAPVEEGAF DMFSPANISP LVAYLSTEKN PWTGQVFKVT GGSIQRLKGW SVTDTAETDG PWSIDLVRDS IEPWK

Validated NT Sequence

ttattacttc cagggctcga tgctgtcgcg caccaggtcg atgctccacg gcccgtcggt ctcggcggtg tcggtgaccg accagccctt gagccgctgg atcgaaccgc ccgtgacctt gaagacctgc ccggtccacg ggttcttctc ggtactcagg tatgccacca gcggggatat gttcgcgggg ctgaacatat cgaacgcccc ctcctccacc ggagccgcga agatggcacc cataccgggg gtcgccagcg tgaggcgggt gcgggcaacc ggcgcaatgg cattcacctt gacgccgtag cgctccagtt cggcggccgc gacacaggac agggccgcga tgcccgcctt ggcggcacca tagttgccct ggcccgggtt cggcagcgtc acaccggagt ccgatgccgt gttcaccacg gtggccttga tatcggcgcc ggccttggcc tgccccttcc agtattcggc ggcgtggcgt agcaccgcga aatgcccctt gaggtggacc tggataaccg cgtcccactg ttcttcctca aggccggcga tgaaaccgtc acggaggatg cccgcgttgt tcaccacggc atccagctgc ccgaaggtct cgacggcctg gttcaccagg ttcgaggcgc cggcccacgt ggagatgttg tcggtattcg caacggcttt gcctcccgcc gcggtgatct cgtccaccac ttcctgtgcg ggcccggcat ccgagccgga gccgtcattg gcgccaccga ggtcgttgac gaccaccgaa gcaccttcct tggcgaagag cagggcgtgc tcacgaccga tgccgcgccc ggcaccggtg atgaccgcca cccgcccgtc aagttgtccc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGQLDGRVA VITGAGRGIG REHALLFAKE GASVVVNDLG GANDGSGSDA GPAQEVVDEI TAAGGKAVAN TDNISTWAGA SNLVNQAVET FGQLDAVVNN AGILRDGFIA GLEEEQWDAV IQVHLKGHFA VLRHAAEYWK GQAKAGADIK ATVVNTASDS GVTLPNPGQG NYGAAKAGIA ALSCVAAAEL ERYGVKVNAI APVARTRLTL ATPGMGAIFA APVEEGAFDM FSPANISPLV AYLSTEKNPW TGQVFKVTGG SIQRLKGWSV TDTAETDGPW SIDLVRDSIE PWK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggacaactt gacgggcggg tggcggtcat caccggtgcc gggcgcggca tcggtcgtga gcacgccctg ctcttcgcca aggaaggtgc ttcggtggtc gtcaacgacc tcggtggcgc caatgacggc tccggctcgg atgccgggcc cgcacaggaa gtggtggacg agatcaccgc ggcgggaggc aaagccgttg cgaataccga caacatctcc acgtgggccg gcgcctcgaa cctggtgaac caggccgtcg agaccttcgg gcagctggat gccgtggtga acaacgcggg catcctccgt gacggtttca tcgccggcct tgaggaagaa cagtgggacg cggttatcca ggtccacctc aaggggcatt tcgcggtgct acgccacgcc gccgaatact ggaaggggca ggccaaggcc ggcgccgata tcaaggccac cgtggtgaac acggcatcgg actccggtgt gacgctgccg aacccgggcc agggcaacta tggtgccgcc aaggcgggca tcgcggccct gtcctgtgtc gcggccgccg aactggagcg ctacggcgtc aaggtgaatg ccattgcgcc ggttgcccgc acccgcctca cgctggcgac ccccggtatg ggtgccatct tcgcggctcc ggtggaggag ggggcgttcg atatgttcag ccccgcgaac atatccccgc tggtggcata cctgagtacc gagaagaacc cgtggaccgg gcaggtcttc aaggtcacgg gcggttcgat ccagcggctc aagggctggt cggtcaccga caccgccgag accgacgggc cgtggagcat cgacctggtg cgcgacagca tcgagccctg gaagtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for MyabA.01582.a.A1.PW30402

PURIFICATION DATe:	12/6/2010
CONCENTRATION:	25.33mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGACAACTT GACGGGCGGG TGGCGGTCAT CACCGGTGCC GGGCGCGGCA TCGGTCGTGA GCACGCCCTG CTCTTCGCCA AGGAAGGTGC TTCGGTGGTC GTCAACGACC TCGGTGGCGC CAATGACGGC TCCGGCTCGG ATGCCGGGCC CGCACAGGAA GTGGTGGACG AGATCACCGC GGCGGGAGGC AAAGCCGTTG CGAATACCGA CAACATCTCC ACGTGGGCCG GCGCCTCGAA CCTGGTGAAC CAGGCCGTCG AGACCTTCGG GCAGCTGGAT GCCGTGGTGA ACAACGCGGG CATCCTCCGT GACGGTTTCA TCGCCGGCCT TGAGGAAGAA CAGTGGGACG CGGTTATCCA GGTCCACCTC AAGGGGCATT TCGCGGTGCT ACGCCACGCC GCCGAATACT GGAAGGGGCA GGCCAAGGCC GGCGCCGATA TCAAGGCCAC CGTGGTGAAC ACGGCATCGG ACTCCGGTGT GACGCTGCCG AACCCGGGCC AGGGCAACTA TGGTGCCGCC AAGGCGGGCA TCGCGGCCCT GTCCTGTGTC GCGGCCGCCG AACTGGAGCG CTACGGCGTC AAGGTGAATG CCATTGCGCC GGTTGCCCGC ACCCGCCTCA CGCTGGCGAC CCCCGGTATG GGTGCCATCT TCGCGGCTCC GGTGGAGGAG GGGGCGTTCG ATATGTTCAG CCCCGCGAAC ATATCCCCGC TGGTGGCATA CCTGAGTACC GAGAAGAACC CGTGGACCGG GCAGGTCTTC AAGGTCACGG GCGGTTCGAT CCAGCGGCTC AAGGGCTGGT CGGTCACCGA CACCGCCGAG ACCGACGGGC CGTGGAGCAT CGACCTGGTG CGCGACAGCA TCGAGCCCTG GAAGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA