MyabA.01566.a

Probable enoyl-CoA hydratase

CENTER ID: MyabA.01566.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01566.a.A1.GE30524 Full length( MyabA.01566.a ) 1 252
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01566.a.A1.PW30401 Full length( MyabA.01566.a ) 1 252

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.3176
RefSeq: YP_001703855.1
UniProt: B1MD80

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEPILLTHT EDRICTITLN RPQARNALST ALGEEIVKAV TAADADENVD VMILTAADPV FCAGVDLKEL GSGDRAESLD PWWPELSKPV IGAINGAAVT GGLELALACD ILIASEKARF ADTHARVGIL PTWGLTTLLP LSVGRGLARR MSLTGDYLSA EDALRAGLVT EVVAHNELLP AAKRLAATIA GNNQPAVREL LASYRRIESE LVGNGLQVAL DDAHRWIDQN SIAEGVEARR TAIMERGRSQ NA
NT Sequence
ATGACCGAAC CAATTTTGTT GACCCACACC GAAGACCGCA TCTGCACCAT CACCCTGAAC CGGCCGCAAG CACGTAACGC CTTGAGCACC GCCCTGGGCG AGGAGATCGT CAAGGCCGTC ACCGCGGCCG ACGCCGACGA GAACGTCGAC GTGATGATCC TGACCGCGGC CGACCCGGTC TTCTGCGCGG GCGTGGACCT CAAGGAGCTG GGCAGTGGCG ATCGTGCCGA GTCCCTGGAC CCGTGGTGGC CCGAACTGAG CAAGCCGGTC ATCGGCGCCA TCAACGGCGC TGCGGTGACC GGCGGTCTGG AGTTGGCACT GGCCTGCGAC ATTCTCATCG CCTCGGAGAA GGCACGATTC GCCGACACCC ATGCGCGCGT TGGCATCTTG CCGACCTGGG GGCTGACCAC CCTGCTGCCG CTCTCGGTGG GGCGGGGCCT GGCCCGGCGG ATGAGCCTGA CCGGCGACTA CCTCTCCGCC GAGGATGCGC TGCGGGCAGG CCTGGTGACC GAGGTGGTCG CGCACAACGA GCTCCTCCCC GCCGCCAAGC GTCTCGCCGC GACCATTGCC GGCAACAACC AGCCCGCGGT CCGCGAACTG CTGGCGTCCT ACCGCCGTAT AGAGTCCGAG CTCGTCGGCA ACGGCCTGCA GGTGGCTCTC GACGATGCTC ACCGGTGGAT CGATCAGAAC TCGATCGCCG AGGGCGTAGA GGCTCGGCGC ACCGCGATCA TGGAGCGCGG ACGCAGCCAG AACGCC
Details for MyabA.01566.a.A1.GE30524
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMTEPILL THTEDRICTI TLNRPQARNA LSTALGEEIV KAVTAADADE NVDVMILTAA DPVFCAGVDL KELGSGDRAE SLDPWWPELS KPVIGAINGA AVTGGLELAL ACDILIASEK ARFADTHARV GILPTWGLTT LLPLSVGRGL ARRMSLTGDY LSAEDALRAG LVTEVVAHNE LLPAAKRLAA TIAGNNQPAV RELLASYRRI ESELVGNGLQ VALDDAHRWI DQNSIAEGVE ARRTAIMERG RSQ
Validated NT Sequence
tctggctgcg tccgcgctcc atgatcgcgg tgcgccgagc ctctacgccc tcggcgatcg agttctgatc gatccaccgg tgagcatcgt cgagagccac ctgcaggccg ttgccgacga gctcggactc tatacggcgg taggacgcca gcagttcgcg gaccgcgggc tggttgttgc cggcaatggt cgcggcgaga cgcttggcgg cggggaggag ctcgttgtgc gcgaccacct cggtcaccag gcctgcccgc agcgcatcct cggcggagag gtagtcgccg gtcaggctca tccgccgggc caggccccgc cccaccgaga gcggcagcag ggtggtcagc ccccaggtcg gcaagatgcc aacgcgcgca tgggtgtcgg cgaatcgtgc cttctccgag gcgatgagaa tgtcgcaggc cagtgccaac tccagaccgc cggtcaccgc agcgccgttg atggcgccga tgaccggctt gctcagttcg ggccaccacg ggtccaggga ctcggcacga tcgccactgc ccagctcctt gaggtccacg cccgcgcaga agaccgggtc ggccgcggtc aggatcatca cgtcgacgtt ctcgtcggcg tcggccgcgg tgacggcctt gacgatctcc tcgcccaggg cggtgctcaa ggcgttacgt gcttgcggcc ggttcagggt gatggtgcag atgcggtctt cggtgtgggt caacaaaatt ggttcggtca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEPILLTH TEDRICTITL NRPQARNALS TALGEEIVKA VTAADADENV DVMILTAADP VFCAGVDLKE LGSGDRAESL DPWWPELSKP VIGAINGAAV TGGLELALAC DILIASEKAR FADTHARVGI LPTWGLTTLL PLSVGRGLAR RMSLTGDYLS AEDALRAGLV TEVVAHNELL PAAKRLAATI AGNNQPAVRE LLASYRRIES ELVGNGLQVA LDDAHRWIDQ NSIAEGVEAR RTAIMERGRS QNA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgaacca attttgttga cccacaccga agaccgcatc tgcaccatca ccctgaaccg gccgcaagca cgtaacgcct tgagcaccgc cctgggcgag gagatcgtca aggccgtcac cgcggccgac gccgacgaga acgtcgacgt gatgatcctg accgcggccg acccggtctt ctgcgcgggc gtggacctca aggagctggg cagtggcgat cgtgccgagt ccctggaccc gtggtggccc gaactgagca agccggtcat cggcgccatc aacggcgctg cggtgaccgg cggtctggag ttggcactgg cctgcgacat tctcatcgcc tcggagaagg cacgattcgc cgacacccat gcgcgcgttg gcatcttgcc gacctggggg ctgaccaccc tgctgccgct ctcggtgggg cggggcctgg cccggcggat gagcctgacc ggcgactacc tctccgccga ggatgcgctg cgggcaggcc tggtgaccga ggtggtcgcg cacaacgagc tcctccccgc cgccaagcgt ctcgccgcga ccattgccgg caacaaccag cccgcggtcc gcgaactgct ggcgtcctac cgccgtatag agtccgagct cgtcggcaac ggcctgcagg tggctctcga cgatgctcac cggtggatcg atcagaactc gatcgccgag ggcgtagagg ctcggcgcac cgcgatcatg gagcgcggac gcagccagaa cgcctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MyabA.01566.a.A1.PW30401
PURIFICATION DATe: 12/6/2010
CONCENTRATION: 28.39mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMTEPILL THTEDRICTI TLNRPQARNA LSTALGEEIV KAVTAADADE NVDVMILTAA DPVFCAGVDL KELGSGDRAE SLDPWWPELS KPVIGAINGA AVTGGLELAL ACDILIASEK ARFADTHARV GILPTWGLTT LLPLSVGRGL ARRMSLTGDY LSAEDALRAG LVTEVVAHNE LLPAAKRLAA TIAGNNQPAV RELLASYRRI ESELVGNGLQ VALDDAHRWI DQNSIAEGVE ARRTAIMERG RSQ
Validated NT Sequence
tctggctgcg tccgcgctcc atgatcgcgg tgcgccgagc ctctacgccc tcggcgatcg agttctgatc gatccaccgg tgagcatcgt cgagagccac ctgcaggccg ttgccgacga gctcggactc tatacggcgg taggacgcca gcagttcgcg gaccgcgggc tggttgttgc cggcaatggt cgcggcgaga cgcttggcgg cggggaggag ctcgttgtgc gcgaccacct cggtcaccag gcctgcccgc agcgcatcct cggcggagag gtagtcgccg gtcaggctca tccgccgggc caggccccgc cccaccgaga gcggcagcag ggtggtcagc ccccaggtcg gcaagatgcc aacgcgcgca tgggtgtcgg cgaatcgtgc cttctccgag gcgatgagaa tgtcgcaggc cagtgccaac tccagaccgc cggtcaccgc agcgccgttg atggcgccga tgaccggctt gctcagttcg ggccaccacg ggtccaggga ctcggcacga tcgccactgc ccagctcctt gaggtccacg cccgcgcaga agaccgggtc ggccgcggtc aggatcatca cgtcgacgtt ctcgtcggcg tcggccgcgg tgacggcctt gacgatctcc tcgcccaggg cggtgctcaa ggcgttacgt gcttgcggcc ggttcagggt gatggtgcag atgcggtctt cggtgtgggt caacaaaatt ggttcggtca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag cc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEPILLTH TEDRICTITL NRPQARNALS TALGEEIVKA VTAADADENV DVMILTAADP VFCAGVDLKE LGSGDRAESL DPWWPELSKP VIGAINGAAV TGGLELALAC DILIASEKAR FADTHARVGI LPTWGLTTLL PLSVGRGLAR RMSLTGDYLS AEDALRAGLV TEVVAHNELL PAAKRLAATI AGNNQPAVRE LLASYRRIES ELVGNGLQVA LDDAHRWIDQ NSIAEGVEAR RTAIMERGRS QNA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGAACCA ATTTTGTTGA CCCACACCGA AGACCGCATC TGCACCATCA CCCTGAACCG GCCGCAAGCA CGTAACGCCT TGAGCACCGC CCTGGGCGAG GAGATCGTCA AGGCCGTCAC CGCGGCCGAC GCCGACGAGA ACGTCGACGT GATGATCCTG ACCGCGGCCG ACCCGGTCTT CTGCGCGGGC GTGGACCTCA AGGAGCTGGG CAGTGGCGAT CGTGCCGAGT CCCTGGACCC GTGGTGGCCC GAACTGAGCA AGCCGGTCAT CGGCGCCATC AACGGCGCTG CGGTGACCGG CGGTCTGGAG TTGGCACTGG CCTGCGACAT TCTCATCGCC TCGGAGAAGG CACGATTCGC CGACACCCAT GCGCGCGTTG GCATCTTGCC GACCTGGGGG CTGACCACCC TGCTGCCGCT CTCGGTGGGG CGGGGCCTGG CCCGGCGGAT GAGCCTGACC GGCGACTACC TCTCCGCCGA GGATGCGCTG CGGGCAGGCC TGGTGACCGA GGTGGTCGCG CACAACGAGC TCCTCCCCGC CGCCAAGCGT CTCGCCGCGA CCATTGCCGG CAACAACCAG CCCGCGGTCC GCGAACTGCT GGCGTCCTAC CGCCGTATAG AGTCCGAGCT CGTCGGCAAC GGCCTGCAGG TGGCTCTCGA CGATGCTCAC CGGTGGATCG ATCAGAACTC GATCGCCGAG GGCGTAGAGG CTCGGCGCAC CGCGATCATG GAGCGCGGAC GCAGCCAGAA CGCCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA