MyabA.01548.b
Methionine aminopeptidase
| CENTER ID: | MyabA.01548.b |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01548.b.A1.GE30523 | Full length( MyabA.01548.b ) | 1 | 264 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3TAV
DEPOSITED: 8/4/2011
DETERMINATION: XRay
CLONE: MyabA.01548.b.A1.GE30523
PROTEIN: MyabA.01548.b.A1.PS01037
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126721 |
| PATRIC ID: | fig|36809.5.peg.3854 |
| RefSeq: | YP_001704511.1 |
| UniProt: | B1MGB2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGLFGRKKTV EQRTPGELDA MAAAGSIVGA ALVAVRDAAK AGVSTLELDQ VAESVIREAG AVPSFLGYHG FPASICSSVN DQVVHGIPSA TAVLADGDLV SIDCGAILDG WHGDSAWTFA VGTVIPSDEA LSEATRLSME AGIAAMIPGN RLTDVSHAIE LGTRAAEKQF DRAFGIVDGY GGHGIGRSMH LDPFLPNEGA PGKGPLLAVG SVLAIEPMLT LGTTQTRVLA DDWTVVTTDG SRAAHWEHTV AVTEAGPRIL TMRP
NT Sequence
GTGGGTCTCT TCGGCCGTAA GAAGACGGTG GAACAGCGCA CGCCCGGTGA GCTCGACGCC ATGGCTGCTG CCGGCTCCAT CGTGGGGGCG GCGCTGGTCG CGGTGCGCGA TGCCGCCAAG GCCGGGGTGT CGACGCTGGA GTTAGATCAG GTCGCCGAGT CCGTGATCCG CGAGGCCGGA GCGGTGCCCT CGTTCCTGGG CTACCACGGC TTTCCGGCGT CCATCTGCTC ATCGGTCAAC GACCAGGTGG TGCACGGAAT ACCTTCCGCG ACAGCGGTAC TTGCCGACGG CGATCTTGTC TCCATCGATT GTGGCGCGAT ACTGGACGGC TGGCACGGGG ATTCCGCATG GACGTTCGCG GTAGGCACCG TCATCCCCAG TGACGAAGCG CTGTCCGAGG CGACGCGTTT GTCGATGGAG GCGGGCATTG CGGCGATGAT CCCGGGTAAC CGGCTCACCG ATGTCTCGCA CGCCATCGAA CTGGGCACCC GCGCCGCCGA GAAGCAATTC GACCGAGCGT TCGGAATCGT GGACGGCTAC GGCGGGCACG GCATCGGGCG CTCGATGCAT CTGGATCCGT TCCTGCCGAA CGAGGGAGCT CCCGGTAAGG GCCCGCTACT CGCGGTGGGA TCGGTACTCG CCATCGAACC GATGCTCACG CTCGGCACCA CCCAGACTCG GGTGCTTGCC GACGATTGGA CCGTTGTCAC CACCGACGGG TCTCGTGCCG CGCACTGGGA GCACACGGTG GCCGTCACCG AGGCCGGCCC CCGCATCCTC ACCATGCGTC CC
Details for MyabA.01548.b.A1.GE30523
| HARVESTED ON: | 9/21/2010 |
| SEQUENCED ON: | 9/28/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGLFGRKK TVEQRTPGEL DAMAAAGSIV GAALVAVRDA AKAGVSTLEL DQVAESVIRE AGAVPSFLGY HGFPASICSS VNDQVVHGIP SATAVLADGD LVSIDCGAIL DGWHGDSAWT FAVGTVIPSD EALSEATRLS MEAGIAAMIP GNRLTDVSHA IELGTRAAEK QFDRAFGIVD GYGGHGIGRS MHLDPFLPNE GAPGKGPLLA VGSVLAIEPM LTLGTTQTRV LADDWTVVTT DGSRAAHWEH TVAVTEAGPR ILTMRP
Validated NT Sequence
ttattaggga cgcatggtga ggatgcgggg gccggcctcg gtgacggcca ccgtgtgctc ccagtgcgcg gcacgagacc cgtcggtggt gacaacggtc caatcgtcgg caagcacccg agtctgggtg gtgccgagcg tgagcatcgg ttcgatggcg agtaccgatc ccaccgcgag tagcgggccc ttaccgggag ctccctcgtt cggcaggaac ggatccagat gcatcgagcg cccgatgccg tgcccgccgt agccgtccac gattccgaac gctcggtcga attgcttctc ggcggcgcgg gtgcccagtt cgatggcgtg cgagacatcg gtgagccggt tacccgggat catcgccgca atgcccgcct ccatcgacaa acgcgtcgcc tcggacagcg cttcgtcact ggggatgacg gtgcctaccg cgaacgtcca tgcggaatcc ccgtgccagc cgtccagtat cgcgccacaa tcgatggaga caagatcgcc gtcggcaagt accgctgtcg cggaaggtat tccgtgcacc acctggtcgt tgaccgatga gcagatggac gccggaaagc cgtggtagcc caggaacgag ggcaccgctc cggcctcgcg gatcacggac tcggcgacct gatctaactc cagcgtcgac accccggcct tggcggcatc gcgcaccgcg accagcgccg cccccacgat ggagccggca gcagccatgg cgtcgagctc accgggcgtg cgctgttcca ccgtcttctt acggccgaag agacccacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGLFGRKK TVEQRTPGEL DAMAAAGSIV GAALVAVRDA AKAGVSTLEL DQVAESVIRE AGAVPSFLGY HGFPASICSS VNDQVVHGIP SATAVLADGD LVSIDCGAIL DGWHGDSAWT FAVGTVIPSD EALSEATRLS MEAGIAAMIP GNRLTDVSHA IELGTRAAEK QFDRAFGIVD GYGGHGIGRS MHLDPFLPNE GAPGKGPLLA VGSVLAIEPM LTLGTTQTRV LADDWTVVTT DGSRAAHWEH TVAVTEAGPR ILTMRP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggtctc ttcggccgta agaagacggt ggaacagcgc acgcccggtg agctcgacgc catggctgct gccggctcca tcgtgggggc ggcgctggtc gcggtgcgcg atgccgccaa ggccggggtg tcgacgctgg agttagatca ggtcgccgag tccgtgatcc gcgaggccgg agcggtgccc tcgttcctgg gctaccacgg ctttccggcg tccatctgct catcggtcaa cgaccaggtg gtgcacggaa taccttccgc gacagcggta cttgccgacg gcgatcttgt ctccatcgat tgtggcgcga tactggacgg ctggcacggg gattccgcat ggacgttcgc ggtaggcacc gtcatcccca gtgacgaagc gctgtccgag gcgacgcgtt tgtcgatgga ggcgggcatt gcggcgatga tcccgggtaa ccggctcacc gatgtctcgc acgccatcga actgggcacc cgcgccgccg agaagcaatt cgaccgagcg ttcggaatcg tggacggcta cggcgggcac ggcatcgggc gctcgatgca tctggatccg ttcctgccga acgagggagc tcccggtaag ggcccgctac tcgcggtggg atcggtactc gccatcgaac cgatgctcac gctcggcacc acccagactc gggtgcttgc cgacgattgg accgttgtca ccaccgacgg gtctcgtgcc gcgcactggg agcacacggt ggccgtcacc gaggccggcc cccgcatcct caccatgcgt ccctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa