MyabA.01548.a

Methionine aminopeptidase

CENTER ID:	MyabA.01548.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01548.a.A1.GE30522	Full length( MyabA.01548.a )		1	255

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126721
PATRIC ID:	fig\|36809.5.peg.126
RefSeq:	YP_001700848.1
UniProt:	B1MED0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIELKSPAEI GRMRVTGEFV ASVLAELSSL AAPGVNLLDL EQRAREMVSD RGAVSCYWDY SPSFGRGPFR NVICLSVNDA VLHGLPHDYV LADGDLLSMD FAVSIDGWVA DSAVSVIVGT PEPEDVRLID TTRAALDAAI GAALPGNRLG DISAAVAAVA EGAGYRINTD FGGHGLGRTM HEDPHVPNKG TAGRGMKLEP GLTLALEPWF GRGTDRLTVD ADGWTLRMAD GSRGAHSEHT IAITEDGPQV LTVQG

NT Sequence

GTGATCGAGC TGAAGTCGCC CGCGGAGATC GGGCGCATGC GCGTGACCGG CGAATTCGTG GCCTCCGTGC TCGCCGAGTT GTCGTCGTTG GCCGCACCGG GTGTCAATCT GCTCGACCTG GAGCAGCGGG CTCGTGAGAT GGTCAGCGAC CGCGGCGCCG TGTCCTGCTA CTGGGACTAC TCCCCCTCGT TCGGCCGCGG CCCATTCCGC AACGTGATCT GCCTTTCCGT CAATGACGCT GTGCTGCATG GACTTCCTCA CGACTATGTG CTTGCCGACG GCGATCTGTT GAGCATGGAC TTTGCGGTGA GCATCGATGG GTGGGTCGCC GACTCCGCAG TCAGTGTCAT CGTCGGGACA CCAGAGCCCG AGGATGTCCG ACTCATCGAC ACCACGCGGG CAGCTCTCGA TGCCGCCATT GGCGCGGCCC TCCCGGGTAA TCGACTCGGC GATATCTCGG CGGCCGTGGC TGCCGTCGCA GAGGGCGCCG GGTACCGGAT CAACACCGAC TTCGGAGGGC ACGGCCTGGG CCGCACCATG CACGAGGATC CCCACGTGCC CAACAAGGGA ACGGCCGGCC GGGGGATGAA GCTGGAGCCG GGCCTCACGC TCGCGCTCGA ACCATGGTTC GGTCGTGGAA CCGACCGGCT CACCGTGGAC GCGGACGGGT GGACCCTGCG GATGGCGGAC GGTTCACGCG GCGCGCACTC CGAGCACACC ATCGCCATCA CCGAGGACGG GCCGCAGGTG CTGACGGTGC AGGGC

Details for MyabA.01548.a.A1.GE30522

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	29kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMVIELKS PAEIGRMRVT GEFVASVLAE LSSLAAPGVN LLDLEQRARE MVSDRGAVSC YWDYSPSFGR GPFRNVICLS VNDAVLHGLP HDYVLADGDL LSMDFAVSID GWVADSAVSV IVGTPEPEDV RLIDTTRAAL DAAIGAALPG NRLGDISAAV AAVAEGAGYR INTDFGGHGL GRTMHEDPHV PNKGTAGRGM KLEPGLTLAL EPWFGRGTDR LTVDADGWTL RMADGSRGAH SEHTIAITED GPQVLT

Validated NT Sequence

caccgtcagc acctgcggcc cgtcctcggt gatggcgatg gtgtgctcgg agtgcgcgcc gcgtgaaccg tccgccatcc gcagggtcca cccgtccgcg tccacggtga gccggtcggt tccacgaccg aaccatggtt cgagcgcgag cgtgaggccc ggctccagct tcatcccccg gccggccgtt cccttgttgg gcacgtgggg atcctcgtgc atggtgcggc ccaggccgtg ccctccgaag tcggtgttga tccggtaccc ggcgccctct gcgacggcag ccacggccgc cgagatatcg ccgagtcgat tacccgggag ggccgcgcca atggcggcat cgagagctgc ccgcgtggtg tcgatgagtc ggacatcctc gggctctggt gtcccgacga tgacactgac tgcggagtcg gcgacccacc catcgatgct caccgcaaag tccatgctca acagatcgcc gtcggcaagc acatagtcgt gaggaagtcc atgcagcaca gcgtcattga cggaaaggca gatcacgttg cggaatgggc cgcggccgaa cgagggggag tagtcccagt agcaggacac ggcgccgcgg tcgctgacca tctcacgagc ccgctgctcc aggtcgagca gattgacacc cggtgcggcc aacgacgaca actcggcgag cacggaggcc acgaattcgc cggtcacgcg catgcgcccg atctccgcgg gcgacttcag ctcgatcacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVIELKSPA EIGRMRVTGE FVASVLAELS SLAAPGVNLL DLEQRAREMV SDRGAVSCYW DYSPSFGRGP FRNVICLSVN DAVLHGLPHD YVLADGDLLS MDFAVSIDGW VADSAVSVIV GTPEPEDVRL IDTTRAALDA AIGAALPGNR LGDISAAVAA VAEGAGYRIN TDFGGHGLGR TMHEDPHVPN KGTAGRGMKL EPGLTLALEP WFGRGTDRLT VDADGWTLRM ADGSRGAHSE HTIAITEDGP QVLTVQG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgatcgag ctgaagtcgc ccgcggagat cgggcgcatg cgcgtgaccg gcgaattcgt ggcctccgtg ctcgccgagt tgtcgtcgtt ggccgcaccg ggtgtcaatc tgctcgacct ggagcagcgg gctcgtgaga tggtcagcga ccgcggcgcc gtgtcctgct actgggacta ctccccctcg ttcggccgcg gcccattccg caacgtgatc tgcctttccg tcaatgacgc tgtgctgcat ggacttcctc acgactatgt gcttgccgac ggcgatctgt tgagcatgga ctttgcggtg agcatcgatg ggtgggtcgc cgactccgca gtcagtgtca tcgtcgggac accagagccc gaggatgtcc gactcatcga caccacgcgg gcagctctcg atgccgccat tggcgcggcc ctcccgggta atcgactcgg cgatatctcg gcggccgtgg ctgccgtcgc agagggcgcc gggtaccgga tcaacaccga cttcggaggg cacggcctgg gccgcaccat gcacgaggat ccccacgtgc ccaacaaggg aacggccggc cgggggatga agctggagcc gggcctcacg ctcgcgctcg aaccatggtt cggtcgtgga accgaccggc tcaccgtgga cgcggacggg tggaccctgc ggatggcgga cggttcacgc ggcgcgcact ccgagcacac catcgccatc accgaggacg ggccgcaggt gctgacggtg cagggctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa