MyabA.01530.d

Probable enoyl-CoA hydratase/isomerase

CENTER ID: MyabA.01530.d
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01530.d.A1.GE30519 Full length( MyabA.01530.d ) 1 259
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01530.d.A1.PS00901 Full length( MyabA.01530.d ) 1 259

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1107
RefSeq: YP_001701813.1
UniProt: B1MJK3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDTLVQYEV EGRAARLTLD SPHNRNALSS GLVRQLREGL QRAAADQTVR AVVLTHTGNT FCAGADLSEA SEGSPQDIAK DRAGQLTALL RDILELPLPV IIAVNGHVRA GGFGLVGAAD IAVAGPDSTF ALTEARIGVA PSIISLTLLP RLTSRAAGRY FVTGEKFDAR VAAEIGLVTQ SVDSAEAVEA AVTSLVDDIA LGSPQGLAES KRLTTAPILA DFDARAVELT ETSARLFVSP EAQEGMLAFL QKRPPAWQV
NT Sequence
ATGAGTGACA CCCTGGTCCA GTACGAGGTC GAAGGCCGTG CCGCACGCCT GACGCTCGAC TCGCCGCACA ACCGTAATGC GTTGTCCAGC GGGCTGGTAC GGCAATTGCG GGAGGGACTG CAGCGGGCCG CCGCGGACCA GACGGTGCGC GCCGTGGTAC TCACCCACAC CGGCAACACC TTCTGTGCCG GAGCGGATCT GTCTGAGGCC TCTGAGGGGT CACCGCAGGA CATCGCCAAG GACCGGGCGG GTCAGCTCAC CGCACTGCTG CGCGACATCC TGGAGCTGCC ACTGCCCGTC ATCATCGCCG TCAATGGCCA TGTGCGGGCC GGCGGTTTCG GTCTCGTCGG TGCGGCCGAT ATCGCGGTGG CGGGTCCGGA CAGCACGTTC GCGCTCACCG AGGCACGCAT CGGGGTGGCG CCATCGATCA TCTCGCTGAC CCTGCTGCCC CGGCTCACCA GCCGGGCCGC GGGACGCTAC TTCGTCACCG GCGAGAAGTT CGACGCGCGG GTGGCCGCGG AGATCGGTTT GGTCACCCAG TCCGTCGACA GCGCCGAGGC TGTCGAGGCC GCGGTCACCT CGTTGGTGGA CGACATCGCG CTGGGTTCAC CACAGGGACT GGCCGAGTCG AAGCGGCTCA CCACTGCGCC GATCCTCGCC GATTTCGACG CGCGTGCAGT CGAGTTGACG GAAACCTCGG CGCGCCTGTT CGTGTCGCCC GAGGCACAGG AGGGCATGCT GGCGTTCCTC CAGAAGCGGC CTCCCGCCTG GCAAGTG
Details for MyabA.01530.d.A1.GE30519
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 29kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDTLVQYE VEGRAARLTL DSPHNRNALS SGLVRQLREG LQRAAADQTV RAVVLTHTGN TFCAGADLSE ASEGSPQDIA KDRAGQLTAL LRDILELPLP VIIAVNGHVR AGGFGLVGAA DIAVAGPDST FALTEARIGV APSIISLTLL PRLTSRAAGR YFVTGEKFDA RVAAEIGLVT QSVDSAEAVE AAVTSLVDDI ALGSPQGLAE SKRLTTAPIL ADFDARAVEL TETSARLFVS PEAQEGMLAF LQKRPPA
Validated NT Sequence
ccaggcggga ggccgcttct ggaggaacgc cagcatgccc tcctgtgcct cgggcgacac gaacaggcgc gccgaggttt ccgtcaactc gactgcacgc gcgtcgaaat cggcgaggat cggcgcagtg gtgagccgct tcgactcggc cagtccctgt ggtgaaccca gcgcgatgtc gtccaccaac gaggtgaccg cggcctcgac agcctcggcg ctgtcgacgg actgggtgac caaaccgatc tccgcggcca cccgcgcgtc gaacttctcg ccggtgacga agtagcgtcc cgcggcccgg ctggtgagcc ggggcagcag ggtcagcgag atgatcgatg gcgccacccc gatgcgtgcc tcggtgagcg cgaacgtgct gtccggaccc gccaccgcga tatcggccgc accgacgaga ccgaaaccgc cggcccgcac atggccattg acggcgatga tgacgggcag tggcagctcc aggatgtcgc gcagcagtgc ggtgagctga cccgcccggt ccttggcgat gtcctgcggt gacccctcag aggcctcaga cagatccgct ccggcacaga aggtgttgcc ggtgtgggtg agtaccacgg cgcgcaccgt ctggtccgcg gcggcccgct gcagtccctc ccgcaattgc cgtaccagcc cgctggacaa cgcattacgg ttgtgcggcg agtcgagcgt caggcgtgcg gcacggcctt cgacctcgta ctggaccagg gtgtcactca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDTLVQYE VEGRAARLTL DSPHNRNALS SGLVRQLREG LQRAAADQTV RAVVLTHTGN TFCAGADLSE ASEGSPQDIA KDRAGQLTAL LRDILELPLP VIIAVNGHVR AGGFGLVGAA DIAVAGPDST FALTEARIGV APSIISLTLL PRLTSRAAGR YFVTGEKFDA RVAAEIGLVT QSVDSAEAVE AAVTSLVDDI ALGSPQGLAE SKRLTTAPIL ADFDARAVEL TETSARLFVS PEAQEGMLAF LQKRPPAWQV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgacacc ctggtccagt acgaggtcga aggccgtgcc gcacgcctga cgctcgactc gccgcacaac cgtaatgcgt tgtccagcgg gctggtacgg caattgcggg agggactgca gcgggccgcc gcggaccaga cggtgcgcgc cgtggtactc acccacaccg gcaacacctt ctgtgccgga gcggatctgt ctgaggcctc tgaggggtca ccgcaggaca tcgccaagga ccgggcgggt cagctcaccg cactgctgcg cgacatcctg gagctgccac tgcccgtcat catcgccgtc aatggccatg tgcgggccgg cggtttcggt ctcgtcggtg cggccgatat cgcggtggcg ggtccggaca gcacgttcgc gctcaccgag gcacgcatcg gggtggcgcc atcgatcatc tcgctgaccc tgctgccccg gctcaccagc cgggccgcgg gacgctactt cgtcaccggc gagaagttcg acgcgcgggt ggccgcggag atcggtttgg tcacccagtc cgtcgacagc gccgaggctg tcgaggccgc ggtcacctcg ttggtggacg acatcgcgct gggttcacca cagggactgg ccgagtcgaa gcggctcacc actgcgccga tcctcgccga tttcgacgcg cgtgcagtcg agttgacgga aacctcggcg cgcctgttcg tgtcgcccga ggcacaggag ggcatgctgg cgttcctcca gaagcggcct cccgcctggc aagtgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MyabA.01530.d.A1.PS00901
PURIFICATION DATe: 12/21/2010
CONCENTRATION: 203mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDTLVQYE VEGRAARLTL DSPHNRNALS SGLVRQLREG LQRAAADQTV RAVVLTHTGN TFCAGADLSE ASEGSPQDIA KDRAGQLTAL LRDILELPLP VIIAVNGHVR AGGFGLVGAA DIAVAGPDST FALTEARIGV APSIISLTLL PRLTSRAAGR YFVTGEKFDA RVAAEIGLVT QSVDSAEAVE AAVTSLVDDI ALGSPQGLAE SKRLTTAPIL ADFDARAVEL TETSARLFVS PEAQEGMLAF LQKRPPA
Validated NT Sequence
ccaggcggga ggccgcttct ggaggaacgc cagcatgccc tcctgtgcct cgggcgacac gaacaggcgc gccgaggttt ccgtcaactc gactgcacgc gcgtcgaaat cggcgaggat cggcgcagtg gtgagccgct tcgactcggc cagtccctgt ggtgaaccca gcgcgatgtc gtccaccaac gaggtgaccg cggcctcgac agcctcggcg ctgtcgacgg actgggtgac caaaccgatc tccgcggcca cccgcgcgtc gaacttctcg ccggtgacga agtagcgtcc cgcggcccgg ctggtgagcc ggggcagcag ggtcagcgag atgatcgatg gcgccacccc gatgcgtgcc tcggtgagcg cgaacgtgct gtccggaccc gccaccgcga tatcggccgc accgacgaga ccgaaaccgc cggcccgcac atggccattg acggcgatga tgacgggcag tggcagctcc aggatgtcgc gcagcagtgc ggtgagctga cccgcccggt ccttggcgat gtcctgcggt gacccctcag aggcctcaga cagatccgct ccggcacaga aggtgttgcc ggtgtgggtg agtaccacgg cgcgcaccgt ctggtccgcg gcggcccgct gcagtccctc ccgcaattgc cgtaccagcc cgctggacaa cgcattacgg ttgtgcggcg agtcgagcgt caggcgtgcg gcacggcctt cgacctcgta ctggaccagg gtgtcactca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDTLVQYE VEGRAARLTL DSPHNRNALS SGLVRQLREG LQRAAADQTV RAVVLTHTGN TFCAGADLSE ASEGSPQDIA KDRAGQLTAL LRDILELPLP VIIAVNGHVR AGGFGLVGAA DIAVAGPDST FALTEARIGV APSIISLTLL PRLTSRAAGR YFVTGEKFDA RVAAEIGLVT QSVDSAEAVE AAVTSLVDDI ALGSPQGLAE SKRLTTAPIL ADFDARAVEL TETSARLFVS PEAQEGMLAF LQKRPPAWQV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTGACACC CTGGTCCAGT ACGAGGTCGA AGGCCGTGCC GCACGCCTGA CGCTCGACTC GCCGCACAAC CGTAATGCGT TGTCCAGCGG GCTGGTACGG CAATTGCGGG AGGGACTGCA GCGGGCCGCC GCGGACCAGA CGGTGCGCGC CGTGGTACTC ACCCACACCG GCAACACCTT CTGTGCCGGA GCGGATCTGT CTGAGGCCTC TGAGGGGTCA CCGCAGGACA TCGCCAAGGA CCGGGCGGGT CAGCTCACCG CACTGCTGCG CGACATCCTG GAGCTGCCAC TGCCCGTCAT CATCGCCGTC AATGGCCATG TGCGGGCCGG CGGTTTCGGT CTCGTCGGTG CGGCCGATAT CGCGGTGGCG GGTCCGGACA GCACGTTCGC GCTCACCGAG GCACGCATCG GGGTGGCGCC ATCGATCATC TCGCTGACCC TGCTGCCCCG GCTCACCAGC CGGGCCGCGG GACGCTACTT CGTCACCGGC GAGAAGTTCG ACGCGCGGGT GGCCGCGGAG ATCGGTTTGG TCACCCAGTC CGTCGACAGC GCCGAGGCTG TCGAGGCCGC GGTCACCTCG TTGGTGGACG ACATCGCGCT GGGTTCACCA CAGGGACTGG CCGAGTCGAA GCGGCTCACC ACTGCGCCGA TCCTCGCCGA TTTCGACGCG CGTGCAGTCG AGTTGACGGA AACCTCGGCG CGCCTGTTCG TGTCGCCCGA GGCACAGGAG GGCATGCTGG CGTTCCTCCA GAAGCGGCCT CCCGCCTGGC AAGTGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA