MyabA.01395.a

Putative thiosulfate sulfurtransferase (SseB)

CENTER ID: MyabA.01395.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01395.a.A1.GE30510 Full length( MyabA.01395.a ) 1 278
MyabA.01395.a.AE1.GE43337 Full length( MyabA.01395.a ) 1 278
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01395.a.AE1.PS38635 Full length( MyabA.01395.a ) 1 278
MyabA.01395.a.A1.PW30387 Full length( MyabA.01395.a ) 1 278

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.102
RefSeq: YP_001700825.1
UniProt: B1MEA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDVLISPSEL ADRLTDFRLL DVRWTVMAPD GRAAYLAGHL PGAVFVDLDV DLADHSVTGR GRHPLPTPQA LAASARRWGL NDGDAVVVYD DWNGQAAARA WWLLRAAGVS NVRILDGGWA AWQRSGGPVA TEEVVPVVGD ITITSLHGLA AVDADAVAAQ AQSPDNLVFD ARAAARYRGD EEPLDPRAGH IPGAVSAPTA ENLTADGTFK PVAELRERFD KLGAGAAPVT VYCGSGVTAT HQIAALAIAG YDAALYPGSW SEWSSDPQRP VATGPNPA
NT Sequence
ATGGACGTGC TCATCTCGCC CTCCGAACTC GCTGACCGGC TAACCGACTT CCGGCTGCTG GACGTGCGCT GGACCGTCAT GGCGCCGGAC GGTCGCGCGG CCTACCTGGC GGGTCACCTT CCCGGCGCGG TGTTCGTGGA TCTCGACGTC GACCTCGCCG ATCACTCCGT CACGGGTCGC GGGCGCCATC CGCTGCCCAC ACCGCAGGCT CTTGCGGCCA GCGCCCGCCG CTGGGGTCTG AACGACGGCG ACGCCGTGGT GGTTTACGAC GATTGGAACG GGCAGGCAGC TGCGCGCGCC TGGTGGCTGC TGCGCGCGGC GGGCGTCTCG AATGTCCGGA TACTCGATGG CGGCTGGGCC GCCTGGCAGC GGTCGGGTGG TCCGGTCGCG ACGGAGGAGG TTGTCCCCGT GGTGGGCGAC ATCACCATCA CCTCGCTGCA CGGTCTTGCC GCCGTCGACG CCGATGCGGT TGCCGCTCAA GCCCAATCAC CAGACAATCT GGTATTCGAC GCACGCGCAG CGGCCCGCTA TCGCGGCGAT GAAGAACCCC TGGACCCTCG TGCCGGGCAC ATCCCTGGTG CCGTGTCCGC TCCCACCGCC GAGAACCTGA CCGCGGACGG CACCTTCAAA CCGGTAGCCG AGTTGCGTGA ACGCTTCGAC AAGTTGGGTG CGGGTGCCGC ACCCGTCACG GTGTACTGCG GCTCGGGCGT CACCGCCACC CATCAGATCG CGGCGCTTGC CATCGCCGGA TACGACGCGG CGCTCTACCC GGGCTCGTGG TCGGAGTGGT CGAGCGACCC GCAACGCCCC GTCGCGACGG GTCCCAACCC GGCC
Details for MyabA.01395.a.A1.GE30510
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDVLISPSE LADRLTDFRL LDVRWTVMAP DGRAAYLAGH LPGAVFVDLD VDLADHSVTG RGRHPLPTPQ ALAASARRWG LNDGDAVVVY DDWNGQAAAR AWWLLRAAGV SNVRILDGGW AAWQRSGGPV ATEEVVPVVG DITITSLHGL AAVDADAVAA QAQSPDNLVF DARAAARYRG DEEPLDPRAG HIPGAVSAPT AENLTADGTF KPVAELRERF DKLGAGAAPV TVYCGSGVTA THQIAALAIA GYDAALYPGS WSEWSSDPQR PVATGP
Validated NT Sequence
tgggacccgt cgcgacgggg cgttgcgggt cgctcgacca ctccgaccac gagcccgggt agagcgccgc gtcgtatccg gcgatggcaa gcgccgcgat ctgatgggtg gcggtgacgc ccgagccgca gtacaccgtg acgggtgcgg cacccgcacc caacttgtcg aagcgttcac gcaactcggc taccggtttg aaggtgccgt ccgcggtcag gttctcggcg gtgggagcgg acacggcacc agggatgtgc ccggcacgag ggtccagggg ttcttcatcg ccgcgatagc gggccgctgc gcgtgcgtcg aataccagat tgtctggtga ttgggcttga gcggcaaccg catcggcgtc gacggcggca agaccgtgca gcgaggtgat ggtgatgtcg cccaccacgg ggacaacctc ctccgtcgcg accggaccac ccgaccgctg ccaggcggcc cagccgccat cgagtatccg gacattcgag acgcccgccg cgcgcagcag ccaccaggcg cgcgcagctg cctgcccgtt ccaatcgtcg taaaccacca cggcgtcgcc gtcgttcaga ccccagcggc gggcgctggc cgcaagagcc tgcggtgtgg gcagcggatg gcgcccgcga cccgtgacgg agtgatcggc gaggtcgacg tcgagatcca cgaacaccgc gccgggaagg tgacccgcca ggtaggccgc gcgaccgtcc ggcgccatga cggtccagcg cacgtccagc agccggaagt cggttagccg gtcagcgagt tcggagggcg agatgagcac gtccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDVLISPSE LADRLTDFRL LDVRWTVMAP DGRAAYLAGH LPGAVFVDLD VDLADHSVTG RGRHPLPTPQ ALAASARRWG LNDGDAVVVY DDWNGQAAAR AWWLLRAAGV SNVRILDGGW AAWQRSGGPV ATEEVVPVVG DITITSLHGL AAVDADAVAA QAQSPDNLVF DARAAARYRG DEEPLDPRAG HIPGAVSAPT AENLTADGTF KPVAELRERF DKLGAGAAPV TVYCGSGVTA THQIAALAIA GYDAALYPGS WSEWSSDPQR PVATGPNPA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgtgctc atctcgccct ccgaactcgc tgaccggcta accgacttcc ggctgctgga cgtgcgctgg accgtcatgg cgccggacgg tcgcgcggcc tacctggcgg gtcaccttcc cggcgcggtg ttcgtggatc tcgacgtcga cctcgccgat cactccgtca cgggtcgcgg gcgccatccg ctgcccacac cgcaggctct tgcggccagc gcccgccgct ggggtctgaa cgacggcgac gccgtggtgg tttacgacga ttggaacggg caggcagctg cgcgcgcctg gtggctgctg cgcgcggcgg gcgtctcgaa tgtccggata ctcgatggcg gctgggccgc ctggcagcgg tcgggtggtc cggtcgcgac ggaggaggtt gtccccgtgg tgggcgacat caccatcacc tcgctgcacg gtcttgccgc cgtcgacgcc gatgcggttg ccgctcaagc ccaatcacca gacaatctgg tattcgacgc acgcgcagcg gcccgctatc gcggcgatga agaacccctg gaccctcgtg ccgggcacat ccctggtgcc gtgtccgctc ccaccgccga gaacctgacc gcggacggca ccttcaaacc ggtagccgag ttgcgtgaac gcttcgacaa gttgggtgcg ggtgccgcac ccgtcacggt gtactgcggc tcgggcgtca ccgccaccca tcagatcgcg gcgcttgcca tcgccggata cgacgcggcg ctctacccgg gctcgtggtc ggagtggtcg agcgacccgc aacgccccgt cgcgacgggt cccaacccgg cctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MyabA.01395.a.AE1.GE43337
HARVESTED ON: 7/31/2019
SEQUENCED ON: 8/7/2019
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MDVLISPSEL ADRLTDFRLL DVRWTVMAPD GRAAYLAGHL PGAVFVDLDV DLADHSVTGR GRHPLPTPQA LAASARRWGL NDGDAVVVYD DWNGQAAARA WWLLRAAGVS NVRILDGGWA AWQRSGGPVA TEEVVPVVGD ITITSLHGLA AVDADAVAAQ AQSPDNLVFD ARAAARYRGD EEPLDPRAGH IPGAVSAPTA ENLTADGTFK PVAELRERFD KLGAGAAPVT VYCGSGVTAT HQIAALAIAG YDAALYPGSW SEWSSDPQRP VATGPNPAAG HHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cggccgggtt gggacccgtc gcgacggggc gttgcgggtc gctcgaccac tccgaccacg agcccgggta gagcgccgcg tcgtatccgg cgatggcaag cgccgcgatc tgatgggtgg cggtgacgcc cgagccgcag tacaccgtga cgggtgcggc acccgcaccc aacttgtcga agcgttcacg caactcggct accggtttga aggtgccgtc cgcggtcagg ttctcggcgg tgggagcgga cacggcacca gggatgtgcc cggcacgagg gtccaggggt tcttcatcgc cgcgatagcg ggccgctgcg cgtgcgtcga ataccagatt gtctggtgat tgggcttgag cggcaaccgc atcggcgtcg acggcggcaa gaccgtgcag cgaggtgatg gtgatgtcgc ccaccacggg gacaacctcc tccgtcgcga ccggaccacc cgaccgctgc caggcggccc agccgccatc gagtatccgg acattcgaga cgcccgccgc gcgcagcagc caccaggcgc gcgcagctgc ctgcccgttc caatcgtcgt aaaccaccac ggcgtcgccg tcgttcagac cccagcggcg ggcgctggcc gcaagagcct gcggtgtggg cagcggatgg cgcccgcgac ccgtgacgga gtgatcggcg aggtcgacgt cgagatccac gaacaccgcg ccgggaaggt gacccgccag gtaggccgcg cgaccgtccg gcgccatgac ggtccagcgc acgtccagca gccggaagtc ggttagccgg tcagcgagtt cggagggcga gatgagcacg tccatgggag aga
Expected Protein Sequence
MDVLISPSEL ADRLTDFRLL DVRWTVMAPD GRAAYLAGHL PGAVFVDLDV DLADHSVTGR GRHPLPTPQA LAASARRWGL NDGDAVVVYD DWNGQAAARA WWLLRAAGVS NVRILDGGWA AWQRSGGPVA TEEVVPVVGD ITITSLHGLA AVDADAVAAQ AQSPDNLVFD ARAAARYRGD EEPLDPRAGH IPGAVSAPTA ENLTADGTFK PVAELRERFD KLGAGAAPVT VYCGSGVTAT HQIAALAIAG YDAALYPGSW SEWSSDPQRP VATGPNPAGH HHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat ggacgtgctc atctcgccct ccgaactcgc tgaccggcta accgacttcc ggctgctgga cgtgcgctgg accgtcatgg cgccggacgg tcgcgcggcc tacctggcgg gtcaccttcc cggcgcggtg ttcgtggatc tcgacgtcga cctcgccgat cactccgtca cgggtcgcgg gcgccatccg ctgcccacac cgcaggctct tgcggccagc gcccgccgct ggggtctgaa cgacggcgac gccgtggtgg tttacgacga ttggaacggg caggcagctg cgcgcgcctg gtggctgctg cgcgcggcgg gcgtctcgaa tgtccggata ctcgatggcg gctgggccgc ctggcagcgg tcgggtggtc cggtcgcgac ggaggaggtt gtccccgtgg tgggcgacat caccatcacc tcgctgcacg gtcttgccgc cgtcgacgcc gatgcggttg ccgctcaagc ccaatcacca gacaatctgg tattcgacgc acgcgcagcg gcccgctatc gcggcgatga agaacccctg gaccctcgtg ccgggcacat ccctggtgcc gtgtccgctc ccaccgccga gaacctgacc gcggacggca ccttcaaacc ggtagccgag ttgcgtgaac gcttcgacaa gttgggtgcg ggtgccgcac ccgtcacggt gtactgcggc tcgggcgtca ccgccaccca tcagatcgcg gcgcttgcca tcgccggata cgacgcggcg ctctacccgg gctcgtggtc ggagtggtcg agcgacccgc aacgccccgt cgcgacgggt cccaacccgg ccgggcacca ccatcatcat cattaacgga tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for MyabA.01395.a.AE1.PS38635
PURIFICATION DATe: 2/11/2020
CONCENTRATION: 12.56mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MDVLISPSEL ADRLTDFRLL DVRWTVMAPD GRAAYLAGHL PGAVFVDLDV DLADHSVTGR GRHPLPTPQA LAASARRWGL NDGDAVVVYD DWNGQAAARA WWLLRAAGVS NVRILDGGWA AWQRSGGPVA TEEVVPVVGD ITITSLHGLA AVDADAVAAQ AQSPDNLVFD ARAAARYRGD EEPLDPRAGH IPGAVSAPTA ENLTADGTFK PVAELRERFD KLGAGAAPVT VYCGSGVTAT HQIAALAIAG YDAALYPGSW SEWSSDPQRP VATGPNPAAG HHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cggccgggtt gggacccgtc gcgacggggc gttgcgggtc gctcgaccac tccgaccacg agcccgggta gagcgccgcg tcgtatccgg cgatggcaag cgccgcgatc tgatgggtgg cggtgacgcc cgagccgcag tacaccgtga cgggtgcggc acccgcaccc aacttgtcga agcgttcacg caactcggct accggtttga aggtgccgtc cgcggtcagg ttctcggcgg tgggagcgga cacggcacca gggatgtgcc cggcacgagg gtccaggggt tcttcatcgc cgcgatagcg ggccgctgcg cgtgcgtcga ataccagatt gtctggtgat tgggcttgag cggcaaccgc atcggcgtcg acggcggcaa gaccgtgcag cgaggtgatg gtgatgtcgc ccaccacggg gacaacctcc tccgtcgcga ccggaccacc cgaccgctgc caggcggccc agccgccatc gagtatccgg acattcgaga cgcccgccgc gcgcagcagc caccaggcgc gcgcagctgc ctgcccgttc caatcgtcgt aaaccaccac ggcgtcgccg tcgttcagac cccagcggcg ggcgctggcc gcaagagcct gcggtgtggg cagcggatgg cgcccgcgac ccgtgacgga gtgatcggcg aggtcgacgt cgagatccac gaacaccgcg ccgggaaggt gacccgccag gtaggccgcg cgaccgtccg gcgccatgac ggtccagcgc acgtccagca gccggaagtc ggttagccgg tcagcgagtt cggagggcga gatgagcacg tccatgggag aga
Expressed Protein Sequence
MDVLISPSEL ADRLTDFRLL DVRWTVMAPD GRAAYLAGHL PGAVFVDLDV DLADHSVTGR GRHPLPTPQA LAASARRWGL NDGDAVVVYD DWNGQAAARA WWLLRAAGVS NVRILDGGWA AWQRSGGPVA TEEVVPVVGD ITITSLHGLA AVDADAVAAQ AQSPDNLVFD ARAAARYRGD EEPLDPRAGH IPGAVSAPTA ENLTADGTFK PVAELRERFD KLGAGAAPVT VYCGSGVTAT HQIAALAIAG YDAALYPGSW SEWSSDPQRP VATGPNPAGH HHHHH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GGACGTGCTC ATCTCGCCCT CCGAACTCGC TGACCGGCTA ACCGACTTCC GGCTGCTGGA CGTGCGCTGG ACCGTCATGG CGCCGGACGG TCGCGCGGCC TACCTGGCGG GTCACCTTCC CGGCGCGGTG TTCGTGGATC TCGACGTCGA CCTCGCCGAT CACTCCGTCA CGGGTCGCGG GCGCCATCCG CTGCCCACAC CGCAGGCTCT TGCGGCCAGC GCCCGCCGCT GGGGTCTGAA CGACGGCGAC GCCGTGGTGG TTTACGACGA TTGGAACGGG CAGGCAGCTG CGCGCGCCTG GTGGCTGCTG CGCGCGGCGG GCGTCTCGAA TGTCCGGATA CTCGATGGCG GCTGGGCCGC CTGGCAGCGG TCGGGTGGTC CGGTCGCGAC GGAGGAGGTT GTCCCCGTGG TGGGCGACAT CACCATCACC TCGCTGCACG GTCTTGCCGC CGTCGACGCC GATGCGGTTG CCGCTCAAGC CCAATCACCA GACAATCTGG TATTCGACGC ACGCGCAGCG GCCCGCTATC GCGGCGATGA AGAACCCCTG GACCCTCGTG CCGGGCACAT CCCTGGTGCC GTGTCCGCTC CCACCGCCGA GAACCTGACC GCGGACGGCA CCTTCAAACC GGTAGCCGAG TTGCGTGAAC GCTTCGACAA GTTGGGTGCG GGTGCCGCAC CCGTCACGGT GTACTGCGGC TCGGGCGTCA CCGCCACCCA TCAGATCGCG GCGCTTGCCA TCGCCGGATA CGACGCGGCG CTCTACCCGG GCTCGTGGTC GGAGTGGTCG AGCGACCCGC AACGCCCCGT CGCGACGGGT CCCAACCCGG CCGGGCACCA CCATCATCAT CATTAACGGA TCCGAATTCG AGCTCCGTCG ACAAGCTTGC GGCCGCACTC GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
Details for MyabA.01395.a.A1.PW30387
PURIFICATION DATe: 12/13/2010
CONCENTRATION: 27.24mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDVLISPSE LADRLTDFRL LDVRWTVMAP DGRAAYLAGH LPGAVFVDLD VDLADHSVTG RGRHPLPTPQ ALAASARRWG LNDGDAVVVY DDWNGQAAAR AWWLLRAAGV SNVRILDGGW AAWQRSGGPV ATEEVVPVVG DITITSLHGL AAVDADAVAA QAQSPDNLVF DARAAARYRG DEEPLDPRAG HIPGAVSAPT AENLTADGTF KPVAELRERF DKLGAGAAPV TVYCGSGVTA THQIAALAIA GYDAALYPGS WSEWSSDPQR PVATGP
Validated NT Sequence
tgggacccgt cgcgacgggg cgttgcgggt cgctcgacca ctccgaccac gagcccgggt agagcgccgc gtcgtatccg gcgatggcaa gcgccgcgat ctgatgggtg gcggtgacgc ccgagccgca gtacaccgtg acgggtgcgg cacccgcacc caacttgtcg aagcgttcac gcaactcggc taccggtttg aaggtgccgt ccgcggtcag gttctcggcg gtgggagcgg acacggcacc agggatgtgc ccggcacgag ggtccagggg ttcttcatcg ccgcgatagc gggccgctgc gcgtgcgtcg aataccagat tgtctggtga ttgggcttga gcggcaaccg catcggcgtc gacggcggca agaccgtgca gcgaggtgat ggtgatgtcg cccaccacgg ggacaacctc ctccgtcgcg accggaccac ccgaccgctg ccaggcggcc cagccgccat cgagtatccg gacattcgag acgcccgccg cgcgcagcag ccaccaggcg cgcgcagctg cctgcccgtt ccaatcgtcg taaaccacca cggcgtcgcc gtcgttcaga ccccagcggc gggcgctggc cgcaagagcc tgcggtgtgg gcagcggatg gcgcccgcga cccgtgacgg agtgatcggc gaggtcgacg tcgagatcca cgaacaccgc gccgggaagg tgacccgcca ggtaggccgc gcgaccgtcc ggcgccatga cggtccagcg cacgtccagc agccggaagt cggttagccg gtcagcgagt tcggagggcg agatgagcac gtccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDVLISPSE LADRLTDFRL LDVRWTVMAP DGRAAYLAGH LPGAVFVDLD VDLADHSVTG RGRHPLPTPQ ALAASARRWG LNDGDAVVVY DDWNGQAAAR AWWLLRAAGV SNVRILDGGW AAWQRSGGPV ATEEVVPVVG DITITSLHGL AAVDADAVAA QAQSPDNLVF DARAAARYRG DEEPLDPRAG HIPGAVSAPT AENLTADGTF KPVAELRERF DKLGAGAAPV TVYCGSGVTA THQIAALAIA GYDAALYPGS WSEWSSDPQR PVATGPNPA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACGTGCTC ATCTCGCCCT CCGAACTCGC TGACCGGCTA ACCGACTTCC GGCTGCTGGA CGTGCGCTGG ACCGTCATGG CGCCGGACGG TCGCGCGGCC TACCTGGCGG GTCACCTTCC CGGCGCGGTG TTCGTGGATC TCGACGTCGA CCTCGCCGAT CACTCCGTCA CGGGTCGCGG GCGCCATCCG CTGCCCACAC CGCAGGCTCT TGCGGCCAGC GCCCGCCGCT GGGGTCTGAA CGACGGCGAC GCCGTGGTGG TTTACGACGA TTGGAACGGG CAGGCAGCTG CGCGCGCCTG GTGGCTGCTG CGCGCGGCGG GCGTCTCGAA TGTCCGGATA CTCGATGGCG GCTGGGCCGC CTGGCAGCGG TCGGGTGGTC CGGTCGCGAC GGAGGAGGTT GTCCCCGTGG TGGGCGACAT CACCATCACC TCGCTGCACG GTCTTGCCGC CGTCGACGCC GATGCGGTTG CCGCTCAAGC CCAATCACCA GACAATCTGG TATTCGACGC ACGCGCAGCG GCCCGCTATC GCGGCGATGA AGAACCCCTG GACCCTCGTG CCGGGCACAT CCCTGGTGCC GTGTCCGCTC CCACCGCCGA GAACCTGACC GCGGACGGCA CCTTCAAACC GGTAGCCGAG TTGCGTGAAC GCTTCGACAA GTTGGGTGCG GGTGCCGCAC CCGTCACGGT GTACTGCGGC TCGGGCGTCA CCGCCACCCA TCAGATCGCG GCGCTTGCCA TCGCCGGATA CGACGCGGCG CTCTACCCGG GCTCGTGGTC GGAGTGGTCG AGCGACCCGC AACGCCCCGT CGCGACGGGT CCCAACCCGG CCTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA