MyabA.01379.a

Probable dehydrogenase/reductase

CENTER ID: MyabA.01379.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01379.a.A1.GE30508 Full length( MyabA.01379.a ) 1 288
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01379.a.A1.PW30385 Full length( MyabA.01379.a ) 1 288

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1259
RefSeq: YP_001701962.1
UniProt: B1MKL6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQQAMKLGY IGLGNMGAPM ARRLVDWPGG LTVFDLRAEA VAPLVEEGAV GASDVADIAD ADLISVTVLD DAQVRQVVGQ VAPKAKPGTI IAIHSTISDT TAVELAQAWR PRDIHVIDAP VSGGAPGAHK GELATMVGAD GETFARVKEA FSKWASLVIH AGAPGAGTRM KLARNLLHFV AFTAAGEAQR LAESCGLDIT DLGKVVRHTD AITGGPGAIM LRDTTEPVTP DSFWLPIFTH VRGLGEKDLS LALALGEEQS VDLPLATLAR EQLAASLGVP HEEGASNG
NT Sequence
ATGACGCAGC AAGCCATGAA ACTGGGATAC ATCGGCCTGG GCAATATGGG GGCGCCCATG GCCCGGCGGC TTGTCGATTG GCCAGGCGGC CTCACGGTCT TCGACCTGCG GGCCGAGGCG GTGGCGCCGC TGGTTGAAGA AGGCGCCGTG GGTGCCTCCG ATGTGGCCGA TATCGCGGAT GCCGACCTCA TCAGTGTCAC CGTGCTCGAT GACGCACAGG TGCGTCAGGT AGTGGGGCAG GTCGCCCCGA AGGCAAAGCC CGGCACCATC ATCGCGATCC ATTCCACCAT TAGCGATACG ACGGCCGTCG AGCTGGCGCA GGCCTGGAGG CCGCGCGATA TTCACGTCAT CGACGCACCG GTGAGCGGCG GTGCGCCGGG TGCGCACAAG GGTGAGCTGG CCACCATGGT GGGTGCCGAC GGCGAGACCT TCGCGCGGGT CAAAGAGGCC TTCTCGAAGT GGGCGTCGTT GGTGATTCAT GCCGGTGCCC CGGGCGCAGG CACCCGAATG AAGCTGGCGC GCAACCTTCT ACATTTTGTG GCCTTCACGG CCGCGGGGGA GGCGCAGCGC TTGGCCGAGA GCTGTGGATT GGACATCACC GACCTGGGCA AGGTCGTGCG GCACACCGAT GCCATCACCG GTGGGCCCGG TGCCATCATG CTGCGCGACA CCACCGAGCC GGTGACGCCG GACAGTTTCT GGCTGCCCAT CTTCACCCAT GTACGGGGCC TGGGCGAGAA GGACCTGAGC CTGGCGCTGG CGCTGGGCGA GGAGCAATCC GTCGACTTGC CGTTGGCGAC GCTGGCCCGT GAGCAGCTGG CGGCGAGTCT TGGTGTGCCA CACGAGGAAG GGGCAAGTAA TGGA
Details for MyabA.01379.a.A1.GE30508
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTQQAMKLG YIGLGNMGAP MARRLVDWPG GLTVFDLRAE AVAPLVEEGA VGASDVADIA DADLISVTVL DDAQVRQVVG QVAPKAKPGT IIAIHSTISD TTAVELAQAW RPRDIHVIDA PVSGGAPGAH KGELATMVGA DGETFARVKE AFSKWASLVI HAGAPGAGTR MKLARNLLHF VAFTAAGEAQ RLAESCGLDI TDLGKVVRHT DAITGGPGAI MLRDTTEPVT PDSFWLPIFT HVRGLGEKDL SLALALGEEQ SVDLPLATLA REQLAASLGV PHEEGAS
Validated NT Sequence
tacttgcccc ttcctcgtgt ggcacaccaa gactcgccgc cagctgctca cgggccagcg tcgccaacgg caagtcgacg gattgctcct cgcccagcgc cagcgccagg ctcaggtcct tctcgcccag gccccgtaca tgggtgaaga tgggcagcca gaaactgtcc ggcgtcaccg gctcggtggt gtcgcgcagc atgatggcac cgggcccacc ggtgatggca tcggtgtgcc gcacgacctt gcccaggtcg gtgatgtcca atccacagct ctcggccaag cgctgcgcct cccccgcggc cgtgaaggcc acaaaatgta gaaggttgcg cgccagcttc attcgggtgc ctgcgcccgg ggcaccggca tgaatcacca acgacgccca cttcgagaag gcctctttga cccgcgcgaa ggtctcgccg tcggcaccca ccatggtggc cagctcaccc ttgtgcgcac ccggcgcacc gccgctcacc ggtgcgtcga tgacgtgaat atcgcgcggc ctccaggcct gcgccagctc gacggccgtc gtatcgctaa tggtggaatg gatcgcgatg atggtgccgg gctttgcctt cggggcgacc tgccccacta cctgacgcac ctgtgcgtca tcgagcacgg tgacactgat gaggtcggca tccgcgatat cggccacatc ggaggcaccc acggcgcctt cttcaaccag cggcgccacc gcctcggccc gcaggtcgaa gaccgtgagg ccgcctggcc aatcgacaag ccgccgggcc atgggcgccc ccatattgcc caggccgatg tatcccagtt tcatggcttg ctgcgtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQQAMKLG YIGLGNMGAP MARRLVDWPG GLTVFDLRAE AVAPLVEEGA VGASDVADIA DADLISVTVL DDAQVRQVVG QVAPKAKPGT IIAIHSTISD TTAVELAQAW RPRDIHVIDA PVSGGAPGAH KGELATMVGA DGETFARVKE AFSKWASLVI HAGAPGAGTR MKLARNLLHF VAFTAAGEAQ RLAESCGLDI TDLGKVVRHT DAITGGPGAI MLRDTTEPVT PDSFWLPIFT HVRGLGEKDL SLALALGEEQ SVDLPLATLA REQLAASLGV PHEEGASNG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcagcaa gccatgaaac tgggatacat cggcctgggc aatatggggg cgcccatggc ccggcggctt gtcgattggc caggcggcct cacggtcttc gacctgcggg ccgaggcggt ggcgccgctg gttgaagaag gcgccgtggg tgcctccgat gtggccgata tcgcggatgc cgacctcatc agtgtcaccg tgctcgatga cgcacaggtg cgtcaggtag tggggcaggt cgccccgaag gcaaagcccg gcaccatcat cgcgatccat tccaccatta gcgatacgac ggccgtcgag ctggcgcagg cctggaggcc gcgcgatatt cacgtcatcg acgcaccggt gagcggcggt gcgccgggtg cgcacaaggg tgagctggcc accatggtgg gtgccgacgg cgagaccttc gcgcgggtca aagaggcctt ctcgaagtgg gcgtcgttgg tgattcatgc cggtgccccg ggcgcaggca cccgaatgaa gctggcgcgc aaccttctac attttgtggc cttcacggcc gcgggggagg cgcagcgctt ggccgagagc tgtggattgg acatcaccga cctgggcaag gtcgtgcggc acaccgatgc catcaccggt gggcccggtg ccatcatgct gcgcgacacc accgagccgg tgacgccgga cagtttctgg ctgcccatct tcacccatgt acggggcctg ggcgagaagg acctgagcct ggcgctggcg ctgggcgagg agcaatccgt cgacttgccg ttggcgacgc tggcccgtga gcagctggcg gcgagtcttg gtgtgccaca cgaggaaggg gcaagtaatg gataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MyabA.01379.a.A1.PW30385
PURIFICATION DATe: 12/13/2010
CONCENTRATION: 26.15mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTQQAMKLG YIGLGNMGAP MARRLVDWPG GLTVFDLRAE AVAPLVEEGA VGASDVADIA DADLISVTVL DDAQVRQVVG QVAPKAKPGT IIAIHSTISD TTAVELAQAW RPRDIHVIDA PVSGGAPGAH KGELATMVGA DGETFARVKE AFSKWASLVI HAGAPGAGTR MKLARNLLHF VAFTAAGEAQ RLAESCGLDI TDLGKVVRHT DAITGGPGAI MLRDTTEPVT PDSFWLPIFT HVRGLGEKDL SLALALGEEQ SVDLPLATLA REQLAASLGV PHEEGAS
Validated NT Sequence
tacttgcccc ttcctcgtgt ggcacaccaa gactcgccgc cagctgctca cgggccagcg tcgccaacgg caagtcgacg gattgctcct cgcccagcgc cagcgccagg ctcaggtcct tctcgcccag gccccgtaca tgggtgaaga tgggcagcca gaaactgtcc ggcgtcaccg gctcggtggt gtcgcgcagc atgatggcac cgggcccacc ggtgatggca tcggtgtgcc gcacgacctt gcccaggtcg gtgatgtcca atccacagct ctcggccaag cgctgcgcct cccccgcggc cgtgaaggcc acaaaatgta gaaggttgcg cgccagcttc attcgggtgc ctgcgcccgg ggcaccggca tgaatcacca acgacgccca cttcgagaag gcctctttga cccgcgcgaa ggtctcgccg tcggcaccca ccatggtggc cagctcaccc ttgtgcgcac ccggcgcacc gccgctcacc ggtgcgtcga tgacgtgaat atcgcgcggc ctccaggcct gcgccagctc gacggccgtc gtatcgctaa tggtggaatg gatcgcgatg atggtgccgg gctttgcctt cggggcgacc tgccccacta cctgacgcac ctgtgcgtca tcgagcacgg tgacactgat gaggtcggca tccgcgatat cggccacatc ggaggcaccc acggcgcctt cttcaaccag cggcgccacc gcctcggccc gcaggtcgaa gaccgtgagg ccgcctggcc aatcgacaag ccgccgggcc atgggcgccc ccatattgcc caggccgatg tatcccagtt tcatggcttg ctgcgtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTQQAMKLG YIGLGNMGAP MARRLVDWPG GLTVFDLRAE AVAPLVEEGA VGASDVADIA DADLISVTVL DDAQVRQVVG QVAPKAKPGT IIAIHSTISD TTAVELAQAW RPRDIHVIDA PVSGGAPGAH KGELATMVGA DGETFARVKE AFSKWASLVI HAGAPGAGTR MKLARNLLHF VAFTAAGEAQ RLAESCGLDI TDLGKVVRHT DAITGGPGAI MLRDTTEPVT PDSFWLPIFT HVRGLGEKDL SLALALGEEQ SVDLPLATLA REQLAASLGV PHEEGASNG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGCAGCAA GCCATGAAAC TGGGATACAT CGGCCTGGGC AATATGGGGG CGCCCATGGC CCGGCGGCTT GTCGATTGGC CAGGCGGCCT CACGGTCTTC GACCTGCGGG CCGAGGCGGT GGCGCCGCTG GTTGAAGAAG GCGCCGTGGG TGCCTCCGAT GTGGCCGATA TCGCGGATGC CGACCTCATC AGTGTCACCG TGCTCGATGA CGCACAGGTG CGTCAGGTAG TGGGGCAGGT CGCCCCGAAG GCAAAGCCCG GCACCATCAT CGCGATCCAT TCCACCATTA GCGATACGAC GGCCGTCGAG CTGGCGCAGG CCTGGAGGCC GCGCGATATT CACGTCATCG ACGCACCGGT GAGCGGCGGT GCGCCGGGTG CGCACAAGGG TGAGCTGGCC ACCATGGTGG GTGCCGACGG CGAGACCTTC GCGCGGGTCA AAGAGGCCTT CTCGAAGTGG GCGTCGTTGG TGATTCATGC CGGTGCCCCG GGCGCAGGCA CCCGAATGAA GCTGGCGCGC AACCTTCTAC ATTTTGTGGC CTTCACGGCC GCGGGGGAGG CGCAGCGCTT GGCCGAGAGC TGTGGATTGG ACATCACCGA CCTGGGCAAG GTCGTGCGGC ACACCGATGC CATCACCGGT GGGCCCGGTG CCATCATGCT GCGCGACACC ACCGAGCCGG TGACGCCGGA CAGTTTCTGG CTGCCCATCT TCACCCATGT ACGGGGCCTG GGCGAGAAGG ACCTGAGCCT GGCGCTGGCG CTGGGCGAGG AGCAATCCGT CGACTTGCCG TTGGCGACGC TGGCCCGTGA GCAGCTGGCG GCGAGTCTTG GTGTGCCACA CGAGGAAGGG GCAAGTAATG GATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA