SSGCID: Target Page

MyabA.01365.c

Probable Short-chain dehydrogenase/reductase

CENTER ID:	MyabA.01365.c
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01365.c.A1.GE30506	Full length( MyabA.01365.c )		1	261

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.4256
RefSeq:	YP_001704905.1
UniProt:	B1MIN0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLLNFDGKV VVVSGIGPGL GAALAIKFAE AGADVVLAAR TQSRLDEVAR QIAATGRRAL AVATDITDEA SAANLVERAT EEFGGIDVLV NNAFSIPSMK SLEKTDYQHI RDSVELTVVG TLRLTQRVAA ALAQSKGAVV NINSMVIRHS QVRYGSYKIA KAALLAMSQS LATELGPQGV RVNSVVPGYM WGENLKGYFE HQAKKYGGTV EQIYEYTAAN SDLKRLPTTD EVANAVLFLA SDLASGITGQ ALDVNCGEFH A

NT Sequence

ATGAGTCTTC TCAATTTCGA CGGCAAGGTT GTCGTCGTAT CCGGTATTGG GCCCGGCCTT GGTGCCGCCC TCGCCATCAA GTTCGCCGAG GCGGGCGCCG ACGTGGTTCT CGCCGCGCGG ACCCAGTCAC GACTCGACGA GGTGGCCAGA CAGATCGCGG CTACCGGACG CAGGGCTCTC GCGGTGGCCA CGGACATCAC CGATGAGGCC AGCGCCGCCA ATCTCGTCGA GCGCGCCACC GAGGAGTTCG GTGGCATCGA CGTTCTCGTC AACAACGCCT TCAGCATTCC GTCGATGAAG TCGCTGGAGA AGACCGACTA TCAGCACATT CGCGATAGCG TCGAACTCAC CGTGGTCGGC ACACTGCGTC TGACGCAACG GGTGGCCGCC GCTCTCGCTC AATCGAAGGG CGCGGTGGTC AATATCAACT CGATGGTCAT CCGGCATTCG CAGGTGCGCT ACGGCAGTTA CAAGATCGCC AAGGCGGCGC TGCTAGCCAT GTCTCAGTCG CTCGCAACGG AATTGGGGCC GCAGGGAGTC CGGGTGAATT CTGTGGTGCC CGGATACATG TGGGGCGAGA ATCTCAAGGG GTATTTCGAA CACCAGGCCA AGAAGTACGG CGGGACCGTC GAGCAGATCT ATGAGTACAC GGCGGCCAAC TCCGATCTCA AGCGATTGCC GACCACCGAT GAGGTGGCCA ATGCCGTGTT GTTCCTCGCG TCCGATCTCG CGAGCGGGAT CACGGGCCAG GCCCTCGATG TCAACTGCGG CGAGTTCCAC GCC

Details for MyabA.01365.c.A1.GE30506

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSLLNFDGK VVVVSGIGPG LGAALAIKFA EAGADVVLAA RTQSRLDEVA RQIAATGRRA LAVATDITDE ASAANLVERA TEEFGGIDVL VNNAFSIPSM KSLEKTDYQH IRDSVELTVV GTLRLTQRVA AALAQSKGAV VNINSMVIRH SQVRYGSYKI AKAALLAMSQ SLATELGPQG VRVNSVVPGY MWGENLKGYF EHQAKKYGGT VEQIYEYTAA NSDLKRLPTT DEVANAVLFL ASDLASGITG QALDVNCGE

Validated NT Sequence

aactcgccgc agttgacatc gagggcctgg cccgtgatcc cgctcgcgag atcggacgcg aggaacaaca cggcattggc cacctcatcg gtggtcggca atcgcttgag atcggagttg gccgccgtgt actcatagat ctgctcgacg gtcccgccgt acttcttggc ctggtgttcg aaatacccct tgagattctc gccccacatg tatccgggca ccacagaatt cacccggact ccctgcggcc ccaattccgt tgcgagcgac tgagacatgg ctagcagcgc cgccttggcg atcttgtaac tgccgtagcg cacctgcgaa tgccggatga ccatcgagtt gatattgacc accgcgccct tcgattgagc gagagcggcg gccacccgtt gcgtcagacg cagtgtgccg accacggtga gttcgacgct atcgcgaatg tgctgatagt cggtcttctc cagcgacttc atcgacggaa tgctgaaggc gttgttgacg agaacgtcga tgccaccgaa ctcctcggtg gcgcgctcga cgagattggc ggcgctggcc tcatcggtga tgtccgtggc caccgcgaga gccctgcgtc cggtagccgc gatctgtctg gccacctcgt cgagtcgtga ctgggtccgc gcggcgagaa ccacgtcggc gcccgcctcg gcgaacttga tggcgagggc ggcaccaagg ccgggcccaa taccggatac gacgacaacc ttgccgtcga aattgagaag actcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtcttctc aatttcgacg gcaaggttgt cgtcgtatcc ggtattgggc ccggccttgg tgccgccctc gccatcaagt tcgccgaggc gggcgccgac gtggttctcg ccgcgcggac ccagtcacga ctcgacgagg tggccagaca gatcgcggct accggacgca gggctctcgc ggtggccacg gacatcaccg atgaggccag cgccgccaat ctcgtcgagc gcgccaccga ggagttcggt ggcatcgacg ttctcgtcaa caacgccttc agcattccgt cgatgaagtc gctggagaag accgactatc agcacattcg cgatagcgtc gaactcaccg tggtcggcac actgcgtctg acgcaacggg tggccgccgc tctcgctcaa tcgaagggcg cggtggtcaa tatcaactcg atggtcatcc ggcattcgca ggtgcgctac ggcagttaca agatcgccaa ggcggcgctg ctagccatgt ctcagtcgct cgcaacggaa ttggggccgc agggagtccg ggtgaattct gtggtgcccg gatacatgtg gggcgagaat ctcaaggggt atttcgaaca ccaggccaag aagtacggcg ggaccgtcga gcagatctat gagtacacgg cggccaactc cgatctcaag cgattgccga ccaccgatga ggtggccaat gccgtgttgt tcctcgcgtc cgatctcgcg agcgggatca cgggccaggc cctcgatgtc aactgcggcg agttccacgc ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa