SSGCID: Target Page

MyabA.01365.b

Possible oxidoreductase

CENTER ID:	MyabA.01365.b
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01365.b.A1.GE30505	Full length( MyabA.01365.b )		1	277

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.4869
RefSeq:	YP_001705512.1
UniProt:	B1MM73

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIRRTVQEF VQRRTLLPDR IDSLIAGPGR DVRGLTVVVT GASAGIGRES VLQLRDQGAR VIGVARRADE LKALAAETGC EWHTCDLSHQ DQIARLLEEL TEQGVDVLVN NAGHSIRRRI VDASDRLHDY QRTMALNYFA AVQLSLGLLP GMIERGRGQF VNVCTWALHA NTFPRFSAYA ASKSALAIFG RSLNAERPHP GVHATNVHFP LVRTEMITPT EEYNDAAALS AQEAGRWIMR AVTHQPVEIS PAVLRAVLPT IDLLSPTASD STIASLT

NT Sequence

GTGTCCATTC GTCGTACCGT CCAAGAGTTT GTGCAGCGCC GCACGTTGCT TCCCGATCGG ATCGACTCCC TGATCGCCGG CCCGGGCCGT GACGTCCGCG GGTTGACCGT CGTAGTCACC GGAGCGTCCG CCGGGATCGG CCGTGAGAGC GTCCTGCAAC TGCGCGACCA GGGCGCGCGA GTGATCGGAG TGGCCCGCCG TGCCGATGAA CTCAAGGCAC TCGCGGCCGA GACCGGCTGC GAATGGCACA CGTGCGATCT GTCCCACCAG GACCAGATCG CACGGTTGTT GGAGGAACTC ACCGAGCAAG GGGTCGACGT ACTGGTGAAC AATGCCGGGC ATTCCATCCG CCGCCGCATT GTCGATGCGT CCGATCGATT GCACGATTAC CAGCGCACCA TGGCGCTGAA CTACTTCGCC GCAGTGCAGC TGAGTCTGGG TTTGTTGCCC GGAATGATCG AGCGGGGCCG CGGTCAATTT GTCAACGTAT GCACATGGGC CCTGCACGCC AACACATTTC CGAGATTCTC CGCGTACGCG GCCTCGAAAA GCGCGCTAGC TATTTTCGGG CGCAGCCTCA ACGCGGAGCG CCCGCATCCC GGAGTGCACG CGACCAACGT GCACTTCCCG CTGGTGCGTA CCGAAATGAT CACTCCCACA GAGGAATACA ATGATGCTGC GGCACTATCC GCCCAGGAAG CCGGGCGTTG GATCATGCGC GCGGTGACCC ATCAGCCCGT GGAGATCAGT CCCGCCGTGC TGCGCGCGGT GCTACCGACA ATCGATCTAC TGTCACCGAC CGCGTCGGAC AGCACCATCG CATCACTCAC G

Details for MyabA.01365.b.A1.GE30505

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	32kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSIRRTVQ EFVQRRTLLP DRIDSLIAGP GRDVRGLTVV VTGASAGIGR ESVLQLRDQG ARVIGVARRA DELKALAAET GCEWHTCDLS HQDQIARLLE ELTEQGVDVL VNNAGHSIRR RIVDASDRLH DYQRTMALNY FAAVQLSLGL LPGMIERGRG QFVNVCTWAL HANTFPRFSA YAASKSALAI FGRSLNAERP HPGVHATNVH FPLVRTEMIT PTEEYNDAAA LSAQEAGRWI MRAVTHQPVE ISPAVLRAVL PTIDLLSPTA XXSTXASLTX

Validated NT Sequence

tttgtttact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgtcc attcgtcgta ccgtccaaga gtttgtgcag cgccgcacgt tgcttcccga tcggatcgac tccctgatcg ccggcccggg ccgtgacgtc cgcgggttga ccgtcgtagt caccggagcg tccgccggga tcggccgtga gagcgtcctg caactgcgcg accagggcgc gcgagtgatc ggagtggccc gccgtgccga tgaactcaag gcactcgcgg ccgagaccgg ctgcgaatgg cacacgtgcg atctgtccca ccaggaccag atcgcacggt tgttggagga actcaccgag caaggggtcg acgtactggt gaacaatgcc gggcattcca tccgccgccg cattgtcgat gcgtccgatc gattgcacga ttaccagcgc accatggcgc tgaactactt cgccgcagtg cagctgagtc tgggtttgtt gcccggaatg atcgagcggg gccgcggtca atttgtcaac gtatgcacat gggccctgca cgccaacaca tttccgagat tctccgcgta cgcggcctcg aaaagcgcgc tagctatttt cgggcgcagc ctcaacgcgg agcgcccgca tcccggagtg cacgcgacca acgtgcactt cccgctggtg cgtaccgaaa tgatcactcc cacagaggaa tacaatgatg ctgcggcact atccgcccag gaagccgggc gttggatcat gcgcgcggtg acccatcagc ccgtggagat cagtcccgcc gtgctgcgcg cggtgctacc gacaatcgat ctactgtcac cgaccgcgtn nnanagcacc ntcgcatcnc tcacgtnnta aacagcacnn ncnagttctg cagccnagct tctcgangat nnngctgcta caaagnncga aangaagctg agtnnnctgc tnncncnnnt nnnnnnnnna nnnnnnnnaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtccatt cgtcgtaccg tccaagagtt tgtgcagcgc cgcacgttgc ttcccgatcg gatcgactcc ctgatcgccg gcccgggccg tgacgtccgc gggttgaccg tcgtagtcac cggagcgtcc gccgggatcg gccgtgagag cgtcctgcaa ctgcgcgacc agggcgcgcg agtgatcgga gtggcccgcc gtgccgatga actcaaggca ctcgcggccg agaccggctg cgaatggcac acgtgcgatc tgtcccacca ggaccagatc gcacggttgt tggaggaact caccgagcaa ggggtcgacg tactggtgaa caatgccggg cattccatcc gccgccgcat tgtcgatgcg tccgatcgat tgcacgatta ccagcgcacc atggcgctga actacttcgc cgcagtgcag ctgagtctgg gtttgttgcc cggaatgatc gagcggggcc gcggtcaatt tgtcaacgta tgcacatggg ccctgcacgc caacacattt ccgagattct ccgcgtacgc ggcctcgaaa agcgcgctag ctattttcgg gcgcagcctc aacgcggagc gcccgcatcc cggagtgcac gcgaccaacg tgcacttccc gctggtgcgt accgaaatga tcactcccac agaggaatac aatgatgctg cggcactatc cgcccaggaa gccgggcgtt ggatcatgcg cgcggtgacc catcagcccg tggagatcag tcccgccgtg ctgcgcgcgg tgctaccgac aatcgatcta ctgtcaccga ccgcgtcgga cagcaccatc gcatcactca cgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa