MyabA.01322.a

Putative CoA-transferase alpha subunit

CENTER ID: MyabA.01322.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01322.a.A1.GE30495 Full length( MyabA.01322.a ) 1 293
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.645
RefSeq: YP_001701357.1
UniProt: B1MH19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGDKRTTLDE VVAELRSGMT IGLGGWGSRR KPMAFVRAIL RSDITDLTVV TYGGPDLGLL CSAGKVKKAY YGFVSLDSPP FYDPWFAKAR TTGAIESREM DEGMVKCGLE AAAARLPFLP IRAGLGSDVM NFWGDELKTV TSPYADSSGQ AETLVAMPAL NLDAAFVHLN LGDATGNAAY TGVDPYFDDL YCLAAERRYL SVERVVSTEE LVKAVPLQSL LLNRMMVDKV VEAPGGAHFT IGAADYGRDE KFQRHYAEAA GDPETWQKFV DTYLSGSEED YQAAVKRFKE AQA
NT Sequence
ATGGGAGACA AGCGCACCAC CCTGGACGAG GTTGTCGCCG AGCTGCGCAG TGGGATGACC ATCGGCCTGG GCGGCTGGGG TTCCCGCCGC AAGCCCATGG CCTTCGTGCG CGCGATTCTG CGTTCGGACA TCACGGATTT GACGGTCGTC ACCTATGGCG GACCGGATCT GGGTCTGCTG TGTTCGGCGG GCAAGGTCAA AAAGGCCTAC TACGGCTTCG TGTCGCTGGA TTCGCCGCCC TTCTACGATC CCTGGTTCGC CAAGGCCCGC ACCACCGGTG CCATCGAGTC CCGCGAGATG GACGAGGGCA TGGTCAAGTG CGGCCTGGAA GCCGCCGCCG CCCGACTGCC GTTCCTGCCC ATTCGCGCCG GACTCGGTTC GGACGTCATG AATTTCTGGG GTGACGAACT CAAGACCGTC ACCTCGCCGT ACGCGGACTC ATCGGGTCAG GCGGAAACCC TGGTGGCCAT GCCCGCCCTG AACCTGGACG CCGCCTTCGT GCACCTGAAC CTGGGCGACG CCACCGGCAA TGCCGCGTAC ACGGGCGTCG ACCCGTACTT CGACGACTTG TACTGCTTGG CCGCCGAGCG CCGCTATCTG TCGGTGGAAA GGGTCGTCTC CACCGAGGAA CTGGTCAAAG CCGTCCCACT GCAATCACTT CTGCTGAACC GAATGATGGT CGACAAGGTC GTTGAGGCGC CCGGTGGCGC ACACTTCACC ATCGGCGCAG CCGACTACGG CCGTGACGAG AAGTTTCAGC GGCATTACGC CGAGGCTGCC GGTGACCCGG AGACCTGGCA GAAGTTCGTC GACACGTACC TATCCGGTTC CGAAGAGGAC TACCAGGCAG CCGTCAAGCG TTTCAAGGAG GCTCAGGCA
Details for MyabA.01322.a.A1.GE30495
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 111
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGDKRTTLD EVVAELRSGM TIGLGGWGSR RKPMAFVRAI LRSDITDLTV VTYGGPDLGL LCSAGKVKKA YYGFVSLDSP PFYDPWFAKA RTTGAIESRE MDEGMVKCGL EAAAARLPFL PIRAGLGSDV MNFWGDELKT VTSPYADSSG QAETLVAMPA LNLDAAFVHL NLGDATGNAA YTGVDPYFDD LYCLAAERRY LSVERVVSTE ELVKAVPLQS LLLNRMMVDK VVEAPGGAHF TIGAADYXRD EKXQRHYXXA AGXPETWXKF VXXYLSXXRX GXPXSRXAFX EAXXXXXHXX VLQPXXRXXX AXXXXXXXCX XXXXXXXXX
Validated NT Sequence
attttgtttn nctttaagaa ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggga gacaagcgca ccaccctgga cgaggttgtc gccgagctgc gcagtgggat gaccatcggc ctgggcggct ggggttcccg ccgcaagccc atggccttcg tgcgcgcgat tctgcgttcg gacatcacgg atttgacggt cgtcacctat ggcggaccgg atctgggtct gctgtgttcg gcgggcaagg tcaaaaaggc ctactacggc ttcgtgtcgc tggattcgcc gcccttctac gatccctggt tcgccaaggc ccgcaccacc ggtgccatcg agtcccgcga gatggacgag ggcatggtca agtgcggcct ggaagccgcc gccgcccgac tgccgttcct gcccattcgc gccggactcg gttcggacgt catgaatttc tggggtgacg aactcaagac cgtcacctcg ccgtacgcgg actcatcggg tcaggcggaa accctggtgg ccatgcccgc cctgaacctg gacgccgcct tcgtgcacct gaacctgggc gacgccaccg gcaatgccgc gtacacgggc gtcgacccgt acttcgacga cttgtactgc ttggccgccg agcgccgcta tctgtcggtg gaaagggtcg tctccaccga ggaactggtc aaagccgtcc cactgcaatc acttctgctg aaccgaatga tggtcgacaa ggtcgttgag gcgcccggtg gcgcacactt caccatcggc gcagccgact acngccgtga cgagaagttn cagcggcatt acnccgangc tgccggtgan ccggagacct ggcanaagtt cgtcganncn tacctatccn ggntccgang aggantaccn ngcagccgtc nagcgtttna ggaggctcnn nnntnatann agcacgnnnn ngttctgcag ccnngntntc gannnnnngn ngctannaan nnnnncngan nnnnctgctn nnnncnnnnn nnnnnnnnna annannana
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGDKRTTLD EVVAELRSGM TIGLGGWGSR RKPMAFVRAI LRSDITDLTV VTYGGPDLGL LCSAGKVKKA YYGFVSLDSP PFYDPWFAKA RTTGAIESRE MDEGMVKCGL EAAAARLPFL PIRAGLGSDV MNFWGDELKT VTSPYADSSG QAETLVAMPA LNLDAAFVHL NLGDATGNAA YTGVDPYFDD LYCLAAERRY LSVERVVSTE ELVKAVPLQS LLLNRMMVDK VVEAPGGAHF TIGAADYGRD EKFQRHYAEA AGDPETWQKF VDTYLSGSEE DYQAAVKRFK EAQA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggagacaag cgcaccaccc tggacgaggt tgtcgccgag ctgcgcagtg ggatgaccat cggcctgggc ggctggggtt cccgccgcaa gcccatggcc ttcgtgcgcg cgattctgcg ttcggacatc acggatttga cggtcgtcac ctatggcgga ccggatctgg gtctgctgtg ttcggcgggc aaggtcaaaa aggcctacta cggcttcgtg tcgctggatt cgccgccctt ctacgatccc tggttcgcca aggcccgcac caccggtgcc atcgagtccc gcgagatgga cgagggcatg gtcaagtgcg gcctggaagc cgccgccgcc cgactgccgt tcctgcccat tcgcgccgga ctcggttcgg acgtcatgaa tttctggggt gacgaactca agaccgtcac ctcgccgtac gcggactcat cgggtcaggc ggaaaccctg gtggccatgc ccgccctgaa cctggacgcc gccttcgtgc acctgaacct gggcgacgcc accggcaatg ccgcgtacac gggcgtcgac ccgtacttcg acgacttgta ctgcttggcc gccgagcgcc gctatctgtc ggtggaaagg gtcgtctcca ccgaggaact ggtcaaagcc gtcccactgc aatcacttct gctgaaccga atgatggtcg acaaggtcgt tgaggcgccc ggtggcgcac acttcaccat cggcgcagcc gactacggcc gtgacgagaa gtttcagcgg cattacgccg aggctgccgg tgacccggag acctggcaga agttcgtcga cacgtaccta tccggttccg aagaggacta ccaggcagcc gtcaagcgtt tcaaggaggc tcaggcataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa