SSGCID: Target Page

MyabA.01285.a

Probable 5'-methylthioadenosine phosphorylase

CENTER ID:	MyabA.01285.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01285.a.A1.GE30494	Full length( MyabA.01285.a )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.4029
RefSeq:	YP_001704684.1
UniProt:	B1MHE9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLAVIGGSGF YSFFDKPVRT VTPATPYGEP SAPITIGLVG GREVAFLPRH GSRHEFSPHT VPYRANMWAL RAVGVRRVFA PCAVGSLTAD LGPGAIVVPD QLVDRTSGRA QTYFDSAGIH VEFADPYCPG LRAVSLQPGT IDGGTMVVVQ GPRFSTRAES RWYASQGFTL VNMTGYPEAV LARELEICYA AIALVTDLDA GVEHGQGVRA VDVFAEFERN IGGFKELVRS AVTATESVDT CGHCRAHDGV TLPIELP

NT Sequence

ATGCTCGCGG TGATAGGTGG AAGCGGTTTC TATTCGTTCT TCGACAAACC GGTGCGGACC GTCACGCCGG CGACACCGTA CGGTGAGCCC AGCGCGCCGA TCACGATCGG GCTGGTGGGC GGGCGGGAGG TCGCCTTTTT ACCACGCCAT GGCAGCCGTC ACGAATTCTC GCCGCACACC GTGCCCTACC GCGCCAACAT GTGGGCGCTG CGGGCCGTGG GTGTGCGCCG GGTCTTCGCG CCCTGCGCGG TGGGCAGCCT GACCGCCGAC CTGGGGCCGG GTGCCATCGT GGTACCCGAC CAGCTGGTGG ACCGCACCAG TGGGCGTGCG CAGACCTATT TCGACAGCGC CGGCATCCAT GTGGAATTCG CCGATCCGTA CTGCCCAGGC CTGCGCGCGG TGTCCCTACA GCCCGGCACC ATTGACGGCG GCACCATGGT GGTGGTACAG GGTCCGCGAT TCTCGACCCG CGCCGAAAGC CGTTGGTATG CGTCGCAGGG TTTCACCCTG GTCAACATGA CCGGATATCC CGAGGCGGTG CTGGCACGCG AGCTCGAAAT CTGCTATGCG GCAATCGCAT TGGTGACAGA TCTGGATGCC GGCGTTGAGC ATGGCCAGGG GGTGCGGGCG GTGGATGTGT TCGCCGAATT CGAGCGCAAC ATCGGCGGAT TCAAGGAATT GGTGCGATCG GCGGTCACCG CCACCGAATC TGTCGATACC TGTGGGCACT GCCGCGCACA CGACGGCGTC ACACTCCCCA TCGAACTGCC C

Details for MyabA.01285.a.A1.GE30494

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLAVIGGSG FYSFFDKPVR TVTPATPYGE PSAPITIGLV GGREVAFLPR HGSRHEFSPH TVPYRANMWA LRAVGVRRVF APCAVGSLTA DLGPGAIVVP DQLVDRTSGR AQTYFDSAGI HVEFADPYCP GLRAVSLQPG TIDGGTMVVV QGPRFSTRAE SRWYASQGFT LVNMTGYPEA VLARELEICY AAIALVTDLD AGVEHGQGVR AVDVFAEFER NIGGFKELVR SAVTATESVD TCGHCRAHDG VTLPIELX

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctcg cggtgatagg tggaagcggt ttctattcgt tcttcgacaa accggtgcgg accgtcacgc cggcgacacc gtacggtgag cccagcgcgc cgatcacgat cgggctggtg ggcgggcggg aggtcgcctt tttaccacgc catggcagcc gtcacgaatt ctcgccgcac accgtgccct accgcgccaa catgtgggcg ctgcgggccg tgggtgtgcg ccgggtcttc gcgccctgcg cggtgggcag cctgaccgcc gacctggggc cgggtgccat cgtggtaccc gaccagctgg tggaccgcac cagtgggcgt gcgcagacct atttcgacag cgccggcatc catgtggaat tcgccgatcc gtactgccca ggcctgcgcg cggtgtccct acagcccggc accattgacg gcggcaccat ggtggtggta cagggtccgc gattctcgac ccgcgccgaa agccgttggt atgcgtcgca gggtttcacc ctggtcaaca tgaccggata tcccgaggcg gtgctggcac gcgagctcga aatctgctat gcggcaatcg cattggtgac agatctggat gccggcgttg agcatggcca gggggtgcgg gcggtggatg tgttcgccga attcgagcgc aacatcggcg gattcaagga attggtgcga tcggcggtca ccgccaccga atctgtcgat acctgtgggc actgccgcgc acacgacggc gtcacactcc ccatcgaact gncctaataa acagcacgaa caagttctgc agccnagctt ctcgaggatc cngctgctaa caaagcccga agaagctgan ttggctgctg ccnccgctna nnnaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctcgcggtg ataggtggaa gcggtttcta ttcgttcttc gacaaaccgg tgcggaccgt cacgccggcg acaccgtacg gtgagcccag cgcgccgatc acgatcgggc tggtgggcgg gcgggaggtc gcctttttac cacgccatgg cagccgtcac gaattctcgc cgcacaccgt gccctaccgc gccaacatgt gggcgctgcg ggccgtgggt gtgcgccggg tcttcgcgcc ctgcgcggtg ggcagcctga ccgccgacct ggggccgggt gccatcgtgg tacccgacca gctggtggac cgcaccagtg ggcgtgcgca gacctatttc gacagcgccg gcatccatgt ggaattcgcc gatccgtact gcccaggcct gcgcgcggtg tccctacagc ccggcaccat tgacggcggc accatggtgg tggtacaggg tccgcgattc tcgacccgcg ccgaaagccg ttggtatgcg tcgcagggtt tcaccctggt caacatgacc ggatatcccg aggcggtgct ggcacgcgag ctcgaaatct gctatgcggc aatcgcattg gtgacagatc tggatgccgg cgttgagcat ggccaggggg tgcgggcggt ggatgtgttc gccgaattcg agcgcaacat cggcggattc aaggaattgg tgcgatcggc ggtcaccgcc accgaatctg tcgatacctg tgggcactgc cgcgcacacg acggcgtcac actccccatc gaactgccct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa