MyabA.01249.d

Probable short-chain dehydrogenase/reductase

CENTER ID: MyabA.01249.d
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01249.d.A1.GE30491 Full length( MyabA.01249.d ) 1 294
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1180
RefSeq: YP_001701886.1
UniProt: B1MKE0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKSTATWSA AKLPSFEGRT VIITGANSGL GLETARELAR VGAHVIMAVR NTEKGNAAQA SIKGSTEVRQ VDVADLASVR AFADTVEGAD ILVNNAGIMM VPPAKTVDGF ESQIGTNHLG AFALTNLLLP KLTDRVIAVS SVAHRAGKID LTDLNYERRR YTRAGAYGAS KLANLLFTKE LQRRLSAAGS PVRALTVHPG VAATELQSHS GNPIFELALK TGNLFAQNAA HGALPTLYAA SQDLPGDTYI GPDGPFEAKG YPTRVGRSGR AQDTETAKGL WRLSEQLTGV SFPL
NT Sequence
GTGAGTAAGT CAACTGCCAC ATGGAGTGCC GCCAAGCTGC CCTCATTCGA GGGCCGCACC GTCATCATCA CCGGAGCCAA TAGCGGACTC GGCTTGGAGA CCGCGCGCGA GCTCGCACGT GTGGGCGCGC ACGTCATCAT GGCGGTGCGC AACACCGAGA AGGGCAACGC CGCCCAGGCT TCGATCAAGG GAAGCACCGA GGTGCGTCAG GTCGACGTGG CAGATCTGGC TTCGGTGCGC GCCTTCGCCG ACACCGTGGA GGGCGCCGAC ATCCTGGTGA ACAACGCGGG CATCATGATG GTGCCACCGG CGAAAACTGT CGATGGCTTC GAAAGCCAAA TCGGGACAAA CCATCTCGGC GCGTTCGCGC TCACCAACCT GCTACTGCCC AAGCTCACCG ACCGGGTGAT CGCGGTGTCG TCCGTCGCGC ATCGTGCCGG GAAAATCGAC CTGACCGACC TCAACTACGA GCGCCGCCGA TACACCCGCG CGGGCGCCTA CGGCGCCTCG AAGCTGGCCA ATCTGCTGTT CACCAAGGAG TTACAGCGCC GCCTGAGCGC AGCCGGATCA CCGGTACGCG CACTGACAGT CCATCCCGGA GTCGCCGCCA CCGAGCTGCA GTCGCACAGC GGGAACCCGA TCTTCGAACT GGCGTTGAAG ACCGGCAACC TCTTCGCCCA GAATGCCGCG CACGGCGCCC TCCCGACGCT GTACGCCGCC TCCCAAGACC TGCCGGGAGA CACATACATC GGGCCCGACG GGCCGTTCGA GGCCAAGGGT TACCCCACGA GGGTGGGCCG CAGCGGTCGG GCGCAGGACA CCGAGACCGC CAAGGGCCTC TGGCGGCTGT CCGAGCAGCT CACCGGGGTT TCTTTCCCGC TT
Details for MyabA.01249.d.A1.GE30491
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 93
PERCENT COVERAGE: 108
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSKSTATW SAAKLPSFEG RTVIITGANS GLGLETAREL ARVGAHVIMA VRNTEKGNAA QASIKGSTEV RQVDVADLAS VRAFADTVEG ADILVNNAGI MMVPPAKTVD GFESQIGTNH LGAFALTNLL LPKLTDRVIA VSSVAHRAGK IDLTDLNYER RRYTRAGAYG ASKLANLLFT KELQRRLSAA GSPVRALTVH PGVAATELQS HSGNPIFELA LKTGNLFAQN AAHGALPTLY AASQXLPGDT YIGPDGPFEA KGYPTRVGRS GRAXXPRPPX ASGGCPXSSP GFLSRXXTAR TSXAXXLXXX PXANKARXXA XXAA
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgag taagtcaact gccacatgga gtgccgccaa gctgccctca ttcgagggcc gcaccgtcat catcaccgga gccaatagcg gactcggctt ggagaccgcg cgcgagctcg cacgtgtggg cgcgcacgtc atcatggcgg tgcgcaacac cgagaagggc aacgccgccc aggcttcgat caagggaagc accgaggtgc gtcaggtcga cgtggcagat ctggcttcgg tgcgcgcctt cgccgacacc gtggagggcg ccgacatcct ggtgaacaac gcgggcatca tgatggtgcc accggcgaaa actgtcgatg gcttcgaaag ccaaatcggg acaaaccatc tcggcgcgtt cgcgctcacc aacctgctac tgcccaagct caccgaccgg gtgatcgcgg tgtcgtccgt cgcgcatcgt gccgggaaaa tcgacctgac cgacctcaac tacgagcgcc gccgatacac ccgcgcgggc gcctacggcg cctcgaagct ggccaatctg ctgttcacca aggagttaca gcgccgcctg agcgcagccg gatcaccggt acgcgcactg acagtccatc ccggagtcgc cgccaccgag ctgcagtcgc acagcgggaa cccgatcttc gaactggcgt tgaagaccgg caacctcttc gcccagaatg ccgcgcacgg cgccctcccg acgctgtacg ccgcctccca aganctgccg ggagacacat acatcgggcc cgacgggccg ttcgaggcca agggttaccc cacgagggtg ggccgcagcg gtcgggcgcn nncaccgaga ccgccanngg cctctggcgg ctgtccganc agctcaccgg ggtttctttc ccgcttnann acagcacgaa caagtnctgc anncnagctn ntcnangnnc cnnctgctaa caaagcccga aannnngctg antnngctgc tnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSKSTATW SAAKLPSFEG RTVIITGANS GLGLETAREL ARVGAHVIMA VRNTEKGNAA QASIKGSTEV RQVDVADLAS VRAFADTVEG ADILVNNAGI MMVPPAKTVD GFESQIGTNH LGAFALTNLL LPKLTDRVIA VSSVAHRAGK IDLTDLNYER RRYTRAGAYG ASKLANLLFT KELQRRLSAA GSPVRALTVH PGVAATELQS HSGNPIFELA LKTGNLFAQN AAHGALPTLY AASQDLPGDT YIGPDGPFEA KGYPTRVGRS GRAQDTETAK GLWRLSEQLT GVSFPL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtaag tcaactgcca catggagtgc cgccaagctg ccctcattcg agggccgcac cgtcatcatc accggagcca atagcggact cggcttggag accgcgcgcg agctcgcacg tgtgggcgcg cacgtcatca tggcggtgcg caacaccgag aagggcaacg ccgcccaggc ttcgatcaag ggaagcaccg aggtgcgtca ggtcgacgtg gcagatctgg cttcggtgcg cgccttcgcc gacaccgtgg agggcgccga catcctggtg aacaacgcgg gcatcatgat ggtgccaccg gcgaaaactg tcgatggctt cgaaagccaa atcgggacaa accatctcgg cgcgttcgcg ctcaccaacc tgctactgcc caagctcacc gaccgggtga tcgcggtgtc gtccgtcgcg catcgtgccg ggaaaatcga cctgaccgac ctcaactacg agcgccgccg atacacccgc gcgggcgcct acggcgcctc gaagctggcc aatctgctgt tcaccaagga gttacagcgc cgcctgagcg cagccggatc accggtacgc gcactgacag tccatcccgg agtcgccgcc accgagctgc agtcgcacag cgggaacccg atcttcgaac tggcgttgaa gaccggcaac ctcttcgccc agaatgccgc gcacggcgcc ctcccgacgc tgtacgccgc ctcccaagac ctgccgggag acacatacat cgggcccgac gggccgttcg aggccaaggg ttaccccacg agggtgggcc gcagcggtcg ggcgcaggac accgagaccg ccaagggcct ctggcggctg tccgagcagc tcaccggggt ttctttcccg ctttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa