MyabA.01249.c

Putative short-chain dehydrogenase/reductase

CENTER ID: MyabA.01249.c
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01249.c.A1.GE30490 Full length( MyabA.01249.c ) 1 307
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.685
RefSeq: YP_001701398.1
UniProt: B1MHS2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRSTTKWST TDIPDQTGRI AIVTGANTGL GLETAKALAA HGAHVVLAVR NAEKGKAAAE AITAAHSNAD VTLQSLDLSS LESVRRASDE LKGRYDKIDL LINNAGVMWT EKSSTADGFE LQFGTNHLGH YALTGLLLER LLPVEGSRVV TVSSIGHRIR ADIHFDDLQW ERDYDRVAAY GQSKLANLLF TYELQRRLAG TNTVALAAHP GGSNTELARN SPLWVRAVFD VVAPVLVQGA DMGALPTLRA ATDPAALGGQ YYGPDGFMEQ RGNPKVVASS EQSYNLDLQR RLWSVSEELT DVVFPVK
NT Sequence
ATGTCGCGAT CCACCACCAA ATGGAGCACC ACCGACATCC CCGATCAGAC CGGCCGCATC GCTATCGTCA CCGGTGCCAA TACCGGACTC GGCCTCGAAA CCGCCAAGGC ACTCGCCGCG CACGGCGCTC ACGTGGTACT CGCCGTCCGC AACGCGGAGA AAGGAAAAGC CGCGGCTGAG GCGATCACCG CGGCCCACTC AAACGCCGAT GTCACCCTGC AGTCATTGGA CCTTTCCTCA CTGGAATCCG TCCGCCGGGC CAGTGATGAG CTCAAGGGGC GCTACGACAA GATCGATCTG CTGATCAACA ACGCCGGCGT CATGTGGACG GAAAAGTCCA GCACCGCCGA CGGCTTCGAG CTGCAGTTCG GCACCAATCA CCTGGGCCAT TACGCACTCA CCGGCCTGCT CTTGGAGCGC CTGTTGCCCG TGGAGGGCTC ACGCGTGGTG ACGGTCAGCA GCATCGGGCA CCGCATCCGC GCCGACATCC ACTTCGATGA CCTGCAATGG GAACGCGACT ACGACCGTGT CGCGGCATAT GGACAGTCGA AGCTGGCCAA CCTGTTGTTC ACCTACGAGC TACAGCGGCG CCTGGCCGGC ACCAACACCG TCGCCCTGGC CGCGCATCCC GGTGGCTCCA ATACCGAACT GGCCCGCAAT TCTCCGCTGT GGGTCCGGGC GGTGTTCGAC GTCGTGGCGC CGGTATTGGT CCAGGGAGCG GATATGGGTG CCCTGCCCAC GCTGCGAGCC GCCACCGACC CGGCTGCCCT GGGCGGCCAG TACTACGGGC CCGACGGATT CATGGAACAG CGCGGTAACC CCAAGGTCGT CGCGTCGAGC GAACAGTCCT ACAACCTCGA TTTGCAGCGT CGGCTGTGGT CGGTATCCGA GGAACTCACC GACGTGGTCT TCCCGGTCAA G
Details for MyabA.01249.c.A1.GE30490
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 107
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSRSTTKWS TTDIPDQTGR IAIVTGANTG LGLETAKALA AHGAHVVLAV RNAEKGKAAA EAITAAHSNA DVTLQSLDLS SLESVRRASD ELKGRYDKID LLINNAGVMW TEKSSTADGF ELQFGTNHLG HYALTGLLLE RLLPVEGSRV VTVSSIGHRI RADIHFDDLQ WERDYDRVAA YGQSKLANLL FTYELQRRLA GTNTVALAAH PGGSNTELAR NSPLWVRAVF DVVAPVLVQG ADMGALPTLR AATDPAALGG QYYXPDGFME QRGNPXVVAS SEQSYNLDLQ RXCGRXPRTX RXXLPXXXXX HXXXXXXXXX AXXRXXXXXX L
Validated NT Sequence
tnnntgttta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcgc gatccaccac caaatggagc accaccgaca tccccgatca gaccggccgc atcgctatcg tcaccggtgc caataccgga ctcggcctcg aaaccgccaa ggcactcgcc gcgcacggcg ctcacgtggt actcgccgtc cgcaacgcgg agaaaggaaa agccgcggct gaggcgatca ccgcggccca ctcaaacgcc gatgtcaccc tgcagtcatt ggacctttcc tcactggaat ccgtccgccg ggccagtgat gagctcaagg ggcgctacga caagatcgat ctgctgatca acaacgccgg cgtcatgtgg acggaaaagt ccagcaccgc cgacggcttc gagctgcagt tcggcaccaa tcacctgggc cattacgcac tcaccggcct gctcttggag cgcctgttgc ccgtggaggg ctcacgcgtg gtgacggtca gcagcatcgg gcaccgcatc cgcgccgaca tccacttcga tgacctgcaa tgggaacgcg actacgaccg tgtcgcggca tatggacagt cgaagctggc caacctgttg ttcacctacg agctacagcg gcgcctggcc ggcaccaaca ccgtcgccct ggccgcgcat cccggtggct ccaataccga actggcccgc aattctccgc tgtgggtccg ggcggtgttc gacgtcgtgg cgccggtatt ggtccaggga gcggatatgg gtgccctgcc cacgctgcga gccgccaccg acccggctgc cctgggcggc cagtactacn ggcccgacgg attcatggaa cagcgcggta accccanggt cgtcgcgtcg agcgaacagt cctacaacct cgatttgcag cgtnnntgtg gtcggnancc gagaactcan cgacntngnc ttcccngnna gtannaanag cacnanantc annnnnnnnc nnnnannnnt gctanaancc gaanngnanc tnnnntngng ctgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSRSTTKWS TTDIPDQTGR IAIVTGANTG LGLETAKALA AHGAHVVLAV RNAEKGKAAA EAITAAHSNA DVTLQSLDLS SLESVRRASD ELKGRYDKID LLINNAGVMW TEKSSTADGF ELQFGTNHLG HYALTGLLLE RLLPVEGSRV VTVSSIGHRI RADIHFDDLQ WERDYDRVAA YGQSKLANLL FTYELQRRLA GTNTVALAAH PGGSNTELAR NSPLWVRAVF DVVAPVLVQG ADMGALPTLR AATDPAALGG QYYGPDGFME QRGNPKVVAS SEQSYNLDLQ RRLWSVSEEL TDVVFPVK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgcgatcc accaccaaat ggagcaccac cgacatcccc gatcagaccg gccgcatcgc tatcgtcacc ggtgccaata ccggactcgg cctcgaaacc gccaaggcac tcgccgcgca cggcgctcac gtggtactcg ccgtccgcaa cgcggagaaa ggaaaagccg cggctgaggc gatcaccgcg gcccactcaa acgccgatgt caccctgcag tcattggacc tttcctcact ggaatccgtc cgccgggcca gtgatgagct caaggggcgc tacgacaaga tcgatctgct gatcaacaac gccggcgtca tgtggacgga aaagtccagc accgccgacg gcttcgagct gcagttcggc accaatcacc tgggccatta cgcactcacc ggcctgctct tggagcgcct gttgcccgtg gagggctcac gcgtggtgac ggtcagcagc atcgggcacc gcatccgcgc cgacatccac ttcgatgacc tgcaatggga acgcgactac gaccgtgtcg cggcatatgg acagtcgaag ctggccaacc tgttgttcac ctacgagcta cagcggcgcc tggccggcac caacaccgtc gccctggccg cgcatcccgg tggctccaat accgaactgg cccgcaattc tccgctgtgg gtccgggcgg tgttcgacgt cgtggcgccg gtattggtcc agggagcgga tatgggtgcc ctgcccacgc tgcgagccgc caccgacccg gctgccctgg gcggccagta ctacgggccc gacggattca tggaacagcg cggtaacccc aaggtcgtcg cgtcgagcga acagtcctac aacctcgatt tgcagcgtcg gctgtggtcg gtatccgagg aactcaccga cgtggtcttc ccggtcaagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa