SSGCID: Target Page

MyabA.01249.a

Putative short-chain dehydrogenase/reductase

CENTER ID:	MyabA.01249.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01249.a.A1.GE30488	Full length( MyabA.01249.a )		1	318

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01249.a.A1.PW30365	Full length( MyabA.01249.a )		1	318

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.2368
RefSeq:	YP_001703060.1
UniProt:	B1MAY5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPAPLQIRHF RESDVPDQTG KTHVITGANN GLGLVAAEAL ARAGARVVLA CRNQQTGRAA LDKVRALGPN ADHALVELDL TSLASVRSAA DAIRTQAPTI DVLLNNAGVM AIPLQRTAEG FEMQIGVNHL GHFVLTDALL PSLLAADAPR VISLGSVAHA QGRNNLKVDD LNFTQRRYNR MTAYRASKLA CMLFGSELAR KAAAAGSSLV SVNVHPGVAA TNLFDSMIPK LPGLHKAFYF GMGLVLQDER QGAESELYAA SMPDVQPDDY LGPTQLTGAR GPVARAPRSK EARDPKLAAQ LWQKSVELTG ADYSRLSG

NT Sequence

ATGCCCGCAC CTCTGCAGAT CAGGCACTTT CGTGAATCGG ATGTTCCCGA CCAGACTGGC AAGACACACG TCATCACCGG TGCGAATAAC GGTCTGGGTC TGGTTGCCGC GGAGGCGCTC GCACGTGCCG GCGCCCGCGT CGTGCTTGCC TGCCGCAACC AGCAGACCGG GCGCGCGGCC CTGGACAAGG TGCGTGCCCT GGGTCCGAAT GCTGACCACG CGCTCGTTGA ATTGGACCTC ACCAGTCTGG CATCGGTGCG ATCGGCCGCG GACGCCATCC GCACCCAGGC GCCGACCATC GACGTGCTGC TGAACAACGC GGGTGTCATG GCGATCCCGT TGCAGCGCAC CGCCGAAGGA TTCGAGATGC AGATCGGCGT GAACCATCTC GGCCACTTCG TGCTGACCGA CGCGCTGCTG CCGTCGCTAC TGGCCGCCGA CGCGCCCCGG GTGATTTCTC TGGGCAGCGT CGCGCACGCG CAGGGACGCA ATAACCTGAA GGTCGACGAC CTGAACTTCA CTCAGCGTCG GTACAACCGG ATGACCGCCT ATCGCGCCTC GAAGCTCGCG TGCATGCTGT TCGGCTCGGA GTTGGCTCGA AAGGCCGCGG CCGCCGGATC CTCGCTGGTG TCGGTGAATG TGCACCCCGG AGTGGCGGCC ACCAACCTGT TCGACTCCAT GATTCCGAAA CTCCCGGGGC TGCATAAGGC CTTCTACTTC GGCATGGGCC TGGTGCTGCA GGACGAGCGC CAAGGTGCGG AGTCCGAGCT GTACGCCGCG TCGATGCCGG ATGTCCAGCC CGACGACTAT CTGGGCCCCA CCCAGTTGAC CGGCGCCAGG GGACCGGTAG CGCGCGCACC CCGGAGCAAG GAAGCACGTG ACCCCAAGCT GGCGGCGCAG TTGTGGCAGA AGTCGGTGGA ACTCACCGGG GCCGACTACT CGCGACTCAG CGGT

Details for MyabA.01249.a.A1.GE30488

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMPAPLQI RHFRESDVPD QTGKTHVITG ANNGLGLVAA EALARAGARV VLACRNQQTG RAALDKVRAL GPNADHALVE LDLTSLASVR SAADAIRTQA PTIDVLLNNA GVMAIPLQRT AEGFEMQIGV NHLGHFVLTD ALLPSLLAAD APRVISLGSV AHAQGRNNLK VDDLNFTQRR YNRMTAYRAS KLACMLFGSE LARKAAAAGS SLVSVNVHPG VAATNLFDSM IPKLPGLHKA FYFGMGLVLQ DERQGAESEL YAASMPDVQP DDYLGPTQLT GARGPVARAP RSKEARDPKL AAQLWQKSVE LTGADYSRLS G

Validated NT Sequence

ttattaaccg ctgagtcgcg agtagtcggc cccggtgagt tccaccgact tctgccacaa ctgcgccgcc agcttggggt cacgtgcttc cttgctccgg ggtgcgcgcg ctaccggtcc cctggcgccg gtcaactggg tggggcccag atagtcgtcg ggctggacat ccggcatcga cgcggcgtac agctcggact ccgcaccttg gcgctcgtcc tgcagcacca ggcccatgcc gaagtagaag gccttatgca gccccgggag tttcggaatc atggagtcga acaggttggt ggccgccact ccggggtgca cattcaccga caccagcgag gatccggcgg ccgcggcctt tcgagccaac tccgagccga acagcatgca cgcgagcttc gaggcgcgat aggcggtcat ccggttgtac cgacgctgag tgaagttcag gtcgtcgacc ttcaggttat tgcgtccctg cgcgtgcgcg acgctgccca gagaaatcac ccggggcgcg tcggcggcca gtagcgacgg cagcagcgcg tcggtcagca cgaagtggcc gagatggttc acgccgatct gcatctcgaa tccttcggcg gtgcgctgca acgggatcgc catgacaccc gcgttgttca gcagcacgtc gatggtcggc gcctgggtgc ggatggcgtc cgcggccgat cgcaccgatg ccagactggt gaggtccaat tcaacgagcg cgtggtcagc attcggaccc agggcacgca ccttgtccag ggccgcgcgc ccggtctgct ggttgcggca ggcaagcacg acgcgggcgc cggcacgtgc gagcgcctcc gcggcaacca gacccagacc gttattcgca ccggtgatga cgtgtgtctt gccagtctgg tcgggaacat ccgattcacg aaagtgcctg atctgcagag gtgcgggcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPAPLQIRH FRESDVPDQT GKTHVITGAN NGLGLVAAEA LARAGARVVL ACRNQQTGRA ALDKVRALGP NADHALVELD LTSLASVRSA ADAIRTQAPT IDVLLNNAGV MAIPLQRTAE GFEMQIGVNH LGHFVLTDAL LPSLLAADAP RVISLGSVAH AQGRNNLKVD DLNFTQRRYN RMTAYRASKL ACMLFGSELA RKAAAAGSSL VSVNVHPGVA ATNLFDSMIP KLPGLHKAFY FGMGLVLQDE RQGAESELYA ASMPDVQPDD YLGPTQLTGA RGPVARAPRS KEARDPKLAA QLWQKSVELT GADYSRLSG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcccgcacct ctgcagatca ggcactttcg tgaatcggat gttcccgacc agactggcaa gacacacgtc atcaccggtg cgaataacgg tctgggtctg gttgccgcgg aggcgctcgc acgtgccggc gcccgcgtcg tgcttgcctg ccgcaaccag cagaccgggc gcgcggccct ggacaaggtg cgtgccctgg gtccgaatgc tgaccacgcg ctcgttgaat tggacctcac cagtctggca tcggtgcgat cggccgcgga cgccatccgc acccaggcgc cgaccatcga cgtgctgctg aacaacgcgg gtgtcatggc gatcccgttg cagcgcaccg ccgaaggatt cgagatgcag atcggcgtga accatctcgg ccacttcgtg ctgaccgacg cgctgctgcc gtcgctactg gccgccgacg cgccccgggt gatttctctg ggcagcgtcg cgcacgcgca gggacgcaat aacctgaagg tcgacgacct gaacttcact cagcgtcggt acaaccggat gaccgcctat cgcgcctcga agctcgcgtg catgctgttc ggctcggagt tggctcgaaa ggccgcggcc gccggatcct cgctggtgtc ggtgaatgtg caccccggag tggcggccac caacctgttc gactccatga ttccgaaact cccggggctg cataaggcct tctacttcgg catgggcctg gtgctgcagg acgagcgcca aggtgcggag tccgagctgt acgccgcgtc gatgccggat gtccagcccg acgactatct gggccccacc cagttgaccg gcgccagggg accggtagcg cgcgcacccc ggagcaagga agcacgtgac cccaagctgg cggcgcagtt gtggcagaag tcggtggaac tcaccggggc cgactactcg cgactcagcg gttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MyabA.01249.a.A1.PW30365

PURIFICATION DATe:	12/13/2010
CONCENTRATION:	28.04mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCCGCACCT CTGCAGATCA GGCACTTTCG TGAATCGGAT GTTCCCGACC AGACTGGCAA GACACACGTC ATCACCGGTG CGAATAACGG TCTGGGTCTG GTTGCCGCGG AGGCGCTCGC ACGTGCCGGC GCCCGCGTCG TGCTTGCCTG CCGCAACCAG CAGACCGGGC GCGCGGCCCT GGACAAGGTG CGTGCCCTGG GTCCGAATGC TGACCACGCG CTCGTTGAAT TGGACCTCAC CAGTCTGGCA TCGGTGCGAT CGGCCGCGGA CGCCATCCGC ACCCAGGCGC CGACCATCGA CGTGCTGCTG AACAACGCGG GTGTCATGGC GATCCCGTTG CAGCGCACCG CCGAAGGATT CGAGATGCAG ATCGGCGTGA ACCATCTCGG CCACTTCGTG CTGACCGACG CGCTGCTGCC GTCGCTACTG GCCGCCGACG CGCCCCGGGT GATTTCTCTG GGCAGCGTCG CGCACGCGCA GGGACGCAAT AACCTGAAGG TCGACGACCT GAACTTCACT CAGCGTCGGT ACAACCGGAT GACCGCCTAT CGCGCCTCGA AGCTCGCGTG CATGCTGTTC GGCTCGGAGT TGGCTCGAAA GGCCGCGGCC GCCGGATCCT CGCTGGTGTC GGTGAATGTG CACCCCGGAG TGGCGGCCAC CAACCTGTTC GACTCCATGA TTCCGAAACT CCCGGGGCTG CATAAGGCCT TCTACTTCGG CATGGGCCTG GTGCTGCAGG ACGAGCGCCA AGGTGCGGAG TCCGAGCTGT ACGCCGCGTC GATGCCGGAT GTCCAGCCCG ACGACTATCT GGGCCCCACC CAGTTGACCG GCGCCAGGGG ACCGGTAGCG CGCGCACCCC GGAGCAAGGA AGCACGTGAC CCCAAGCTGG CGGCGCAGTT GTGGCAGAAG TCGGTGGAAC TCACCGGGGC CGACTACTCG CGACTCAGCG GTTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA