SSGCID: Target Page

MyabA.01204.a

Putative short-chain dehydrogenase/reductase

CENTER ID:	MyabA.01204.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01204.a.A2.GE30486	Mature protein( MyabA.01204.a )		24	249

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.1076
RefSeq:	YP_001701783.1
UniProt:	B1MJH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTRQKILITG ASSGLGAEMA RQFAAKGRDL ALCARRTEAL EELKAELLGA NPGIKVAVRA LDVTNHESVP VVFGELRDEL GGLDRVVVNA GIAKGWHLGG GKSWANIQTI ETNLIGALVQ IEAALALFKE QGYGHLVLIS SVTAAKGLPG TKAAYAASKA GLSSLGESLR SEYARGPIKV STIEPGYIQT DLSAKSPTTP MMVDTLTGVT AMVNAIEKEP GRAAVPRWPW EPVMAVMRLL PPRLAGRLA

NT Sequence

GTGACGCGAC AGAAGATCCT CATCACCGGG GCGAGCTCCG GCCTGGGTGC CGAGATGGCC CGTCAGTTCG CTGCGAAGGG ACGGGACCTG GCGCTGTGCG CCCGGCGCAC CGAGGCGCTC GAAGAACTCA AGGCAGAGTT GCTGGGCGCC AACCCGGGAA TCAAGGTCGC GGTGCGGGCG CTGGACGTCA CCAACCACGA ATCCGTGCCG GTCGTATTCG GCGAGCTGCG CGACGAGCTC GGCGGCCTGG ATCGGGTAGT GGTCAACGCC GGCATCGCCA AGGGTTGGCA CCTCGGTGGC GGCAAGTCGT GGGCCAACAT CCAGACCATC GAAACCAACC TCATCGGTGC ACTGGTACAG ATCGAGGCGG CGCTAGCCCT GTTCAAGGAA CAGGGCTACG GGCACCTGGT CCTCATCTCC TCGGTGACAG CGGCCAAGGG GCTGCCTGGC ACCAAAGCCG CCTATGCGGC CAGCAAGGCC GGACTTTCCT CGCTGGGTGA ATCACTGCGA TCCGAATATG CCCGCGGCCC AATCAAAGTC AGCACCATTG AGCCTGGCTA CATACAGACC GATCTGAGCG CCAAGTCGCC GACCACCCCG ATGATGGTCG ACACACTGAC CGGCGTGACG GCAATGGTGA ACGCCATCGA GAAGGAGCCC GGCCGCGCGG CGGTGCCGCG ATGGCCGTGG GAACCGGTGA TGGCGGTCAT GCGGTTGCTG CCGCCCCGGC TGGCGGGGCG CCTCGCG

Details for MyabA.01204.a.A2.GE30486

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAAKGRDLA LCARRTEALE ELKAELLGAN PGIKVAVRAL DVTNHESVPV VFGELRDELG GLDRVVVNAG IAKGWHLGGG KSWANIQTIE TNLIGALVQI EAALALFKEQ GYGHLVLISS VTAAKGLPGT KAAYAASKAG LSSLGESLRS EYARGPIKVS TIEPGYIQTD LSAKSPTTPM MVDTLTGVTA MVNAIEKEPG RAAVPRWPWE PVMAVMRLLP PRLAGRLA

Validated NT Sequence

tttgtttact ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggctgcg aagggacggg acctggcgct gtgcgcccgg cgcaccgagg cgctcgaaga actcaaggca gagttgctgg gcgccaaccc gggaatcaag gtcgcggtgc gggcgctgga cgtcaccaac cacgaatccg tgccggtcgt attcggcgag ctgcgcgacg agctcggcgg cctggatcgg gtagtggtca acgccggcat cgccaagggt tggcacctcg gtggcggcaa gtcgtgggcc aacatccaga ccatcgaaac caacctcatc ggtgcactgg tacagatcga ggcggcgcta gccctgttca aggaacaggg ctacgggcac ctggtcctca tctcctcggt gacagcggcc aaggggctgc ctggcaccaa agccgcctat gcggccagca aggccggact ttcctcgctg ggtgaatcac tgcgatccga atatgcccgc ggcccaatca aagtcagcac cattgagcct ggctacatac agaccgatct gagcgccaag tcgccgacca ccccgatgat ggtcgacaca ctgaccggcg tgacggcaat ggtgaacgcc atcgagaagg agcccggccg cgcggcggtg ccgcgatggc cgtgggaacc ggtgatggcg gtcatgcggt tgctgccgcc ccggctggcg gggcgcctcg cgtaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgcgaag ggacgggacc tggcgctgtg cgcccggcgc accgaggcgc tcgaagaact caaggcagag ttgctgggcg ccaacccggg aatcaaggtc gcggtgcggg cgctggacgt caccaaccac gaatccgtgc cggtcgtatt cggcgagctg cgcgacgagc tcggcggcct ggatcgggta gtggtcaacg ccggcatcgc caagggttgg cacctcggtg gcggcaagtc gtgggccaac atccagacca tcgaaaccaa cctcatcggt gcactggtac agatcgaggc ggcgctagcc ctgttcaagg aacagggcta cgggcacctg gtcctcatct cctcggtgac agcggccaag gggctgcctg gcaccaaagc cgcctatgcg gccagcaagg ccggactttc ctcgctgggt gaatcactgc gatccgaata tgcccgcggc ccaatcaaag tcagcaccat tgagcctggc tacatacaga ccgatctgag cgccaagtcg ccgaccaccc cgatgatggt cgacacactg accggcgtga cggcaatggt gaacgccatc gagaaggagc ccggccgcgc ggcggtgccg cgatggccgt gggaaccggt gatggcggtc atgcggttgc tgccgccccg gctggcgggg cgcctcgcgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa