MyabA.01117.b

Probable acyl-CoA dehydrogenase FadE

CENTER ID:	MyabA.01117.b
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01117.b.A1.GE30373	Full length( MyabA.01117.b )		1	392

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_239169
PATRIC ID:	fig\|36809.5.peg.4236
RefSeq:	YP_001704885.1
UniProt:	B1MIL0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHIAYTPEQE ELRRELRAYF DKLLTPERRE ALASNQGEYG SGNVYRETVE QMGADGWLAL GWPKEFGGQD RSVMDQLIFT DEAAIAGAPV PFLTINSVAP TIMHFGTDEQ KKFFLPKIAA GKLHFSIGYS EPGAGTDLAS LRTSAVRDGD DYIVNGQKMW TSLIEYADYI WLAVRTNTEV KKHRGISMLI VPTTAEGFSY TKVHTMAGPG TSATYYQDVR VPVTSRVGEE NAGWKLVTNQ LNHERVALVS SAPIITALRE VREWAQNTKG PGGRIIDAEW VQLNLARVHA KAEYLKLINW ELASTTDAAP SPADASATKV FGTELATEAY RLLMEVLGTA ATLRQDSPGA QLRGRVERMH RACLILTFGG GTNEVQRDII AMTALGQPAA SR

NT Sequence

ATGCATATCG CCTACACGCC CGAGCAGGAA GAGCTGCGCC GCGAGCTACG CGCATACTTC GACAAACTGC TGACCCCGGA GCGGCGGGAG GCTCTGGCGT CGAACCAAGG TGAATACGGC AGCGGCAACG TTTATCGCGA GACCGTCGAG CAGATGGGAG CCGACGGATG GCTGGCCCTC GGCTGGCCCA AGGAATTCGG CGGCCAGGAC CGCTCGGTCA TGGATCAGCT GATCTTCACC GACGAGGCCG CCATCGCCGG CGCACCGGTG CCGTTCCTGA CGATCAACAG CGTTGCGCCG ACGATCATGC ATTTCGGCAC CGATGAGCAG AAGAAGTTCT TCTTGCCGAA GATCGCCGCG GGCAAGTTGC ACTTCTCCAT CGGGTACTCC GAGCCGGGCG CCGGAACCGA CCTCGCCTCG CTGCGCACCT CGGCGGTACG CGATGGCGAT GACTACATCG TCAACGGTCA GAAGATGTGG ACCAGTCTCA TCGAGTACGC CGACTACATC TGGCTGGCGG TGCGCACCAA CACCGAGGTC AAGAAACACC GCGGTATCTC CATGCTCATC GTTCCCACCA CGGCCGAGGG CTTCTCGTAC ACCAAGGTGC ACACCATGGC CGGGCCCGGC ACCAGCGCCA CCTACTACCA GGATGTGCGC GTCCCGGTCA CGAGCCGGGT CGGCGAGGAG AACGCCGGCT GGAAGCTGGT CACCAATCAG CTCAACCACG AGCGGGTGGC TCTGGTTTCG TCGGCGCCGA TCATCACCGC GCTGCGCGAG GTCCGCGAGT GGGCCCAGAA CACCAAGGGC CCGGGTGGCC GGATCATCGA CGCGGAATGG GTGCAGCTGA ACCTGGCGCG CGTGCATGCC AAGGCCGAAT ATCTCAAGCT CATCAACTGG GAGCTGGCCT CCACTACCGA CGCGGCCCCC TCTCCCGCCG ACGCCTCGGC CACCAAGGTC TTTGGTACCG AGTTGGCCAC CGAGGCCTAC CGCCTTCTCA TGGAGGTCCT CGGCACCGCC GCGACGCTGC GCCAGGACTC GCCCGGCGCG CAGCTGCGCG GCCGCGTCGA GCGGATGCAC CGTGCCTGCT TGATTCTCAC CTTCGGCGGT GGCACCAACG AGGTGCAACG CGACATCATC GCGATGACCG CTCTTGGCCA ACCTGCGGCA AGCCGC

Details for MyabA.01117.b.A1.GE30373

HARVESTED ON:	9/8/2010
SEQUENCED ON:	9/17/2010
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	80
PERCENT COVERAGE:	96

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMHIAYTPEQ EELRRELRAY FDKLLTPERR EALASNQGEY GSGNVYRETV EQMGADGWLA LGWPKEFGGQ DRSVMDQLIF TDEAAIAGAP VPFLTINSVA PTIMHFGTDE QKKFFLPKIA AGKLHFSIGY SEPGAGTDLA SLRTSAVRDG DDYIVNGQKM WTSLIEYADY IWLAVRTNTE VKKHRGISML IVPTTAEGFS YTKVHTMAGP GTSATYYQDV RVPVTSRVGE ENAGWKLVTN QLNHERVALV SSAPIITALR XVREWXQNTX GPGGXIIDAE WVQLNLARVH AKXEYLKLIN WXLGLXXRRX PPLPXTPXPX XSXXPXXXXX XXXPXHXXXX XXPXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX

Validated NT Sequence

ttttgtttac tttangaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcatat cgcctacacg cccgagcagg aagagctgcg ccgcgagcta cgcgcatact tcgacaaact gctgaccccg gagcggcggg aggctctggc gtcgaaccaa ggtgaatacg gcagcggcaa cgtttatcgc gagaccgtcg agcagatggg agccgacgga tggctggccc tcggctggcc caaggaattc ggcggccagg accgctcggt catggatcag ctgatcttca ccgacgaggc cgccatcgcc ggcgcaccgg tgccgttcct gacgatcaac agcgttgcgc cgacgatcat gcatttcggc accgatgagc agaagaagtt cttcttgccg aagatcgccg cgggcaagtt gcacttctcc atcgggtact ccgagccggg cgccggaacc gacctcgcct cgctgcgcac ctcggcggta cgcgatggcg atgactacat cgtcaacggt cagaagatgt ggaccagtct catcgagtac gccgactaca tctggctggc ggtgcgcacc aacaccgagg tcaagaaaca ccgcggtatc tccatgctca tcgttcccac cacggccgag ggcttctcgt acaccaaggt gcacaccatg gccgggcccg gcaccagcgc cacctactac caggatgtgc gcgtcccggt cacgagccgg gtcggcgagg agaacgccgg ctggaagctg gtcaccaatc agctcaacca cgagcgggtg gctctggttt cgtcggcgcc gatcatcacc gcgctgcgcg nggtccgcga gtgggnccag aacaccaang gcccnggtgg ncngatcatc gacgcggaat gggtgcagct gaacctggcg cgcgtgcatg ccaaggncga atatctcaag ctcatcaact ggganctggg nctnnactan cgacgnngnc cccctctccc gncgacgccn cngccancnn ngtcttngnt accgnntngn cnnncnnang ncnnancncc nnctcatgnn gnncntcnnn ancnnccnnn nnncnncncn nnnnnnnnng nnnnnannnn cnnnnncnnn nnnnnnnnnn ncnnnnnntn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nannnnnntn c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMHIAYTPEQ EELRRELRAY FDKLLTPERR EALASNQGEY GSGNVYRETV EQMGADGWLA LGWPKEFGGQ DRSVMDQLIF TDEAAIAGAP VPFLTINSVA PTIMHFGTDE QKKFFLPKIA AGKLHFSIGY SEPGAGTDLA SLRTSAVRDG DDYIVNGQKM WTSLIEYADY IWLAVRTNTE VKKHRGISML IVPTTAEGFS YTKVHTMAGP GTSATYYQDV RVPVTSRVGE ENAGWKLVTN QLNHERVALV SSAPIITALR EVREWAQNTK GPGGRIIDAE WVQLNLARVH AKAEYLKLIN WELASTTDAA PSPADASATK VFGTELATEA YRLLMEVLGT AATLRQDSPG AQLRGRVERM HRACLILTFG GGTNEVQRDI IAMTALGQPA ASR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcatatcgcc tacacgcccg agcaggaaga gctgcgccgc gagctacgcg catacttcga caaactgctg accccggagc ggcgggaggc tctggcgtcg aaccaaggtg aatacggcag cggcaacgtt tatcgcgaga ccgtcgagca gatgggagcc gacggatggc tggccctcgg ctggcccaag gaattcggcg gccaggaccg ctcggtcatg gatcagctga tcttcaccga cgaggccgcc atcgccggcg caccggtgcc gttcctgacg atcaacagcg ttgcgccgac gatcatgcat ttcggcaccg atgagcagaa gaagttcttc ttgccgaaga tcgccgcggg caagttgcac ttctccatcg ggtactccga gccgggcgcc ggaaccgacc tcgcctcgct gcgcacctcg gcggtacgcg atggcgatga ctacatcgtc aacggtcaga agatgtggac cagtctcatc gagtacgccg actacatctg gctggcggtg cgcaccaaca ccgaggtcaa gaaacaccgc ggtatctcca tgctcatcgt tcccaccacg gccgagggct tctcgtacac caaggtgcac accatggccg ggcccggcac cagcgccacc tactaccagg atgtgcgcgt cccggtcacg agccgggtcg gcgaggagaa cgccggctgg aagctggtca ccaatcagct caaccacgag cgggtggctc tggtttcgtc ggcgccgatc atcaccgcgc tgcgcgaggt ccgcgagtgg gcccagaaca ccaagggccc gggtggccgg atcatcgacg cggaatgggt gcagctgaac ctggcgcgcg tgcatgccaa ggccgaatat ctcaagctca tcaactggga gctggcctcc actaccgacg cggccccctc tcccgccgac gcctcggcca ccaaggtctt tggtaccgag ttggccaccg aggcctaccg ccttctcatg gaggtcctcg gcaccgccgc gacgctgcgc caggactcgc ccggcgcgca gctgcgcggc cgcgtcgagc ggatgcaccg tgcctgcttg attctcacct tcggcggtgg caccaacgag gtgcaacgcg acatcatcgc gatgaccgct cttggccaac ctgcggcaag ccgctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa