MyabA.01097.i

Probable short-chain dehydrogenase/reductase

CENTER ID: MyabA.01097.i
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01097.i.A1.GE30368 Full length( MyabA.01097.i ) 1 276
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1428
RefSeq: YP_001702129.1
UniProt: B1MLP8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEGFSGKVAV VTGAGSGIGR ALAVELARSG AKIAISDIDN EGLAETERQI KSLGGDVRSD RLNVAEREAF LLYADAIKEH FGTVNQIYNN AGIDFHGELE ASEFKDIERI IDVDFWGVVN GTKAFLPHLI ASGDGHVVNV SSIFGVMACP GQSSYNAAKF AVRGFTESLR QEMIIGKKPV AVTCVHPGGI KTNVARNATV AEGYDHAEFT KLFDMLARTR PQAAARIILT AVRKKKPRVL VGPDAKAIDI LVRLTGARYQ DLFVLGERFL MPKRSH
NT Sequence
ATGGAGGGCT TCAGCGGCAA AGTCGCCGTG GTGACCGGTG CCGGTTCGGG CATCGGACGC GCACTGGCCG TCGAGCTGGC ACGATCGGGC GCCAAGATCG CCATCAGCGA TATCGACAAC GAGGGCTTGG CCGAAACCGA ACGCCAGATC AAGAGCCTTG GCGGTGATGT CCGATCCGAT CGCCTCAACG TCGCGGAGCG CGAAGCCTTT CTGCTCTACG CCGACGCCAT CAAGGAGCAC TTCGGCACGG TCAACCAGAT CTACAACAAC GCCGGCATCG ACTTCCATGG CGAACTGGAA GCCAGCGAGT TCAAGGACAT CGAGCGCATC ATCGATGTCG ACTTCTGGGG CGTCGTCAAC GGCACCAAGG CCTTCCTGCC ACACCTGATC GCCTCCGGCG ACGGCCACGT CGTCAACGTC TCCTCGATCT TCGGAGTCAT GGCATGCCCG GGCCAAAGCT CCTACAACGC AGCGAAATTC GCGGTGCGCG GCTTCACCGA GTCACTGCGC CAAGAAATGA TCATCGGCAA GAAGCCCGTC GCCGTCACCT GCGTGCACCC CGGCGGCATC AAGACCAACG TGGCCCGCAA CGCCACGGTC GCCGAAGGCT ACGACCATGC TGAATTCACG AAGTTGTTCG ACATGCTCGC CAGAACCAGA CCGCAGGCCG CGGCGCGAAT CATTCTGACC GCCGTACGCA AGAAGAAGCC CCGCGTGCTT GTGGGGCCGG ACGCGAAAGC AATCGACATT CTGGTCCGGC TGACCGGTGC GCGCTACCAG GATCTGTTCG TACTCGGCGA GCGGTTCCTG ATGCCCAAAC GTTCCCAT
Details for MyabA.01097.i.A1.GE30368
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMEGFSGK VAVVTGAGSG IGRALAVELA RSGAKIAISD IDNEGLAETE RQIKSLGGDV RSDRLNVAER EAFLLYADAI KEHFGTVNQI YNNAGIDFHG ELEASEFKDI ERIIDVDFWG VVNGTKAFLP HLIASGDGHV VNVSSIFGVM ACPGQSSYNA AKFAVRGFTE SLRQEMIIGK KPVAVTCVHP GGIKTNVARN ATVAEGYDHA EFTKLFDMLA RTRPQAAARI ILTAVRKKKP RVLVGPDAKA IDILVRLTGA RYQDLFVLGE RFLMPKRSH
Validated NT Sequence
ttattaatgg gaacgtttgg gcatcaggaa ccgctcgccg agtacgaaca gatcctggta gcgcgcaccg gtcagccgga ccagaatgtc gattgctttc gcgtccggcc ccacaagcac gcggggcttc ttcttgcgta cggcggtcag aatgattcgc gccgcggcct gcggtctggt tctggcgagc atgtcgaaca acttcgtgaa ttcagcatgg tcgtagcctt cggcgaccgt ggcgttgcgg gccacgttgg tcttgatgcc gccggggtgc acgcaggtga cggcgacggg cttcttgccg atgatcattt cttggcgcag tgactcggtg aagccgcgca ccgcgaattt cgctgcgttg taggagcttt ggcccgggca tgccatgact ccgaagatcg aggagacgtt gacgacgtgg ccgtcgccgg aggcgatcag gtgtggcagg aaggccttgg tgccgttgac gacgccccag aagtcgacat cgatgatgcg ctcgatgtcc ttgaactcgc tggcttccag ttcgccatgg aagtcgatgc cggcgttgtt gtagatctgg ttgaccgtgc cgaagtgctc cttgatggcg tcggcgtaga gcagaaaggc ttcgcgctcc gcgacgttga ggcgatcgga tcggacatca ccgccaaggc tcttgatctg gcgttcggtt tcggccaagc cctcgttgtc gatatcgctg atggcgatct tggcgcccga tcgtgccagc tcgacggcca gtgcgcgtcc gatgcccgaa ccggcaccgg tcaccacggc gactttgccg ctgaagccct ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEGFSGKVA VVTGAGSGIG RALAVELARS GAKIAISDID NEGLAETERQ IKSLGGDVRS DRLNVAEREA FLLYADAIKE HFGTVNQIYN NAGIDFHGEL EASEFKDIER IIDVDFWGVV NGTKAFLPHL IASGDGHVVN VSSIFGVMAC PGQSSYNAAK FAVRGFTESL RQEMIIGKKP VAVTCVHPGG IKTNVARNAT VAEGYDHAEF TKLFDMLART RPQAAARIIL TAVRKKKPRV LVGPDAKAID ILVRLTGARY QDLFVLGERF LMPKRSH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagggcttc agcggcaaag tcgccgtggt gaccggtgcc ggttcgggca tcggacgcgc actggccgtc gagctggcac gatcgggcgc caagatcgcc atcagcgata tcgacaacga gggcttggcc gaaaccgaac gccagatcaa gagccttggc ggtgatgtcc gatccgatcg cctcaacgtc gcggagcgcg aagcctttct gctctacgcc gacgccatca aggagcactt cggcacggtc aaccagatct acaacaacgc cggcatcgac ttccatggcg aactggaagc cagcgagttc aaggacatcg agcgcatcat cgatgtcgac ttctggggcg tcgtcaacgg caccaaggcc ttcctgccac acctgatcgc ctccggcgac ggccacgtcg tcaacgtctc ctcgatcttc ggagtcatgg catgcccggg ccaaagctcc tacaacgcag cgaaattcgc ggtgcgcggc ttcaccgagt cactgcgcca agaaatgatc atcggcaaga agcccgtcgc cgtcacctgc gtgcaccccg gcggcatcaa gaccaacgtg gcccgcaacg ccacggtcgc cgaaggctac gaccatgctg aattcacgaa gttgttcgac atgctcgcca gaaccagacc gcaggccgcg gcgcgaatca ttctgaccgc cgtacgcaag aagaagcccc gcgtgcttgt ggggccggac gcgaaagcaa tcgacattct ggtccggctg accggtgcgc gctaccagga tctgttcgta ctcggcgagc ggttcctgat gcccaaacgt tcccattaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa