MyabA.01097.h

Probable short-chain dehydrogenase/reductase

CENTER ID: MyabA.01097.h
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01097.h.A1.PW30252 Full length( MyabA.01097.h ) 1 278

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1427
RefSeq: YP_001702128.1
UniProt: B1MLP7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEGFSGKVAV VTGAGSGIGR ALAVELARSG AKIAISDIDN EGLAETERQV KALGAEVRAD RLNVAEREAF LLYADAIKDH FGKVNQIYNN AGIAYQGEVE ESQFKDIERI IDVDFWGVVN GTKAFLPHLI ASGDGHVVNV SSLFGVLSMP GQSAYNSAKF AVRGFTESLR QEMIIGKKPV AVTCVHPGGI KTAIARNSTT AEGYDQQAMA AMFDKYLANT SPEAAARIIL TAVRKKKPRV LVGPDAKILD VIVRLTGARY QDIFSVVTRF ILPRPGKK
NT Sequence
ATGGAAGGCT TCAGCGGCAA GGTGGCCGTG GTGACCGGCG CGGGTTCGGG TATCGGACGC GCACTGGCCG TCGAGCTGGC ACGATCGGGC GCCAAGATCG CCATCAGCGA TATCGACAAC GAGGGCTTGG CCGAAACCGA ACGCCAGGTC AAGGCGCTCG GCGCCGAGGT CCGTGCGGAT CGTCTCAATG TCGCGGAGCG CGAAGCCTTC CTGCTCTACG CCGACGCCAT CAAGGACCAC TTCGGCAAGG TCAACCAGAT CTACAACAAC GCCGGCATCG CATACCAGGG CGAGGTAGAG GAAAGCCAGT TCAAGGACAT CGAGCGCATC ATCGATGTCG ACTTCTGGGG CGTCGTCAAC GGCACCAAGG CCTTCCTGCC ACACCTGATC GCCTCCGGCG ACGGCCACGT CGTCAACGTC TCCAGCCTGT TCGGCGTGCT GTCCATGCCC GGCCAAAGTG CTTACAACTC GGCCAAGTTC GCGGTGCGCG GCTTCACCGA GTCACTGCGC CAAGAAATGA TCATCGGCAA GAAGCCCGTC GCCGTCACCT GCGTGCACCC CGGCGGCATC AAGACCGCCA TCGCGCGCAA CTCCACCACG GCCGAGGGCT ACGACCAGCA GGCGATGGCC GCGATGTTCG ACAAGTACCT GGCGAACACC AGCCCGGAGG CCGCTGCCCG CATCATCCTG ACCGCCGTAC GCAAGAAGAA GCCGCGGGTA CTGGTCGGCC CGGACGCCAA GATCCTGGAC GTCATCGTCC GGCTCACCGG TGCCCGTTAC CAGGACATCT TCTCCGTGGT GACCCGCTTC ATCCTGCCCC GGCCGGGTAA GAAG
Details for MyabA.01097.h.A1.PW30252
PURIFICATION DATe: 3/25/2011
CONCENTRATION: 26.57mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 20
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MXHHHHHHMG XXKXRPRXXF DGRLQRQGGR GDXRGFGYRT RTGRRAGTIG RQDRHQRYRQ RGLGRNRTPG QGARRRGPCG SSQCRGARSL PALRRRHQGP LRQGQPDLQQ RRHRIPGRGR GKPVQGHRAH HRCRLLGRRQ RHQGLPATPD RLRRRPRRQR LQPVRRAVHA RPKCLQLGQV RGARLHRVTA PRNDHRQEAR RRHLRAPRRH QDRHRAQLHH GRGLRPAGDG RDVRQVPGEH QPGGRCPHHP DRRTQEEAAG TGRPGRQDPG RHRPAHRCPL PGHLLRGDPL HPAPAG
Validated NT Sequence
cnttcgngct ttgttagcag ccggatcctc gagaagcttg gctgcagaac ttgttcgtgc tgtttattac ttcttacccg gccggggcag gatgaagcgg gtcaccacgg agaagatgtc ctggtaacgg gcaccggtga gccggacgat gacgtccagg atcttggcgt ccgggccgac cagtacccgc ggcttcttct tgcgtacggc ggtcaggatg atgcgggcag cggcctccgg gctggtgttc gccaggtact tgtcgaacat cgcggccatc gcctgctggt cgtagccctc ggccgtggtg gagttgcgcg cgatggcggt cttgatgccg ccggggtgca cgcaggtgac ggcgacgggc ttcttgccga tgatcatttc ttggcgcagt gactcggtga agccgcgcac cgcgaacttg gccgagttgt aagcactttg gccgggcatg gacagcacgc cgaacaggct ggagacgttg acgacgtggc cgtcgccgga ggcgatcagg tgtggcagga aggccttggt gccgttgacg acgccccaga agtcgacatc gatgatgcgc tcgatgtcct tgaactggct ttcctctacc tcgccctggt atgcgatgcc ggcgttgttg tagatctggt tgaccttgcc gaagtggtcc ttgatggcgt cggcgtagag caggaaggct tcgcgctccg cgacattgag acgatccgca cggacctcgg cgccgagcgc cttgacctgg cgttcggttt cggccaagcc ctcgttgtcg atatcgctga tggcgatctt ggcgcccgat cgtgccagct cgacggccag tgcgcgtccg atacccgaac ccgcgccngt caccacggcc accttgccgc tgaagccttc catcgaaccn nnccctgggt ctgancttcc nnnnacccat atgatggtga tggtgatgan ccatggtata tctccttctt naagtnaann naaatnattt ctannnggna ancnnnnnnn nnnnnnnnnn nnnnnggnnn nnnnnnnnna aaaaaaaaaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEGFSGKVA VVTGAGSGIG RALAVELARS GAKIAISDID NEGLAETERQ VKALGAEVRA DRLNVAEREA FLLYADAIKD HFGKVNQIYN NAGIAYQGEV EESQFKDIER IIDVDFWGVV NGTKAFLPHL IASGDGHVVN VSSLFGVLSM PGQSAYNSAK FAVRGFTESL RQEMIIGKKP VAVTCVHPGG IKTAIARNST TAEGYDQQAM AAMFDKYLAN TSPEAAARII LTAVRKKKPR VLVGPDAKIL DVIVRLTGAR YQDIFSVVTR FILPRPGKK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAAGGCTTC AGCGGCAAGG TGGCCGTGGT GACCGGCGCG GGTTCGGGTA TCGGACGCGC ACTGGCCGTC GAGCTGGCAC GATCGGGCGC CAAGATCGCC ATCAGCGATA TCGACAACGA GGGCTTGGCC GAAACCGAAC GCCAGGTCAA GGCGCTCGGC GCCGAGGTCC GTGCGGATCG TCTCAATGTC GCGGAGCGCG AAGCCTTCCT GCTCTACGCC GACGCCATCA AGGACCACTT CGGCAAGGTC AACCAGATCT ACAACAACGC CGGCATCGCA TACCAGGGCG AGGTAGAGGA AAGCCAGTTC AAGGACATCG AGCGCATCAT CGATGTCGAC TTCTGGGGCG TCGTCAACGG CACCAAGGCC TTCCTGCCAC ACCTGATCGC CTCCGGCGAC GGCCACGTCG TCAACGTCTC CAGCCTGTTC GGCGTGCTGT CCATGCCCGG CCAAAGTGCT TACAACTCGG CCAAGTTCGC GGTGCGCGGC TTCACCGAGT CACTGCGCCA AGAAATGATC ATCGGCAAGA AGCCCGTCGC CGTCACCTGC GTGCACCCCG GCGGCATCAA GACCGCCATC GCGCGCAACT CCACCACGGC CGAGGGCTAC GACCAGCAGG CGATGGCCGC GATGTTCGAC AAGTACCTGG CGAACACCAG CCCGGAGGCC GCTGCCCGCA TCATCCTGAC CGCCGTACGC AAGAAGAAGC CGCGGGTACT GGTCGGCCCG GACGCCAAGA TCCTGGACGT CATCGTCCGG CTCACCGGTG CCCGTTACCA GGACATCTTC TCCGTGGTGA CCCGCTTCAT CCTGCCCCGG CCGGGTAAGA AGTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA