SSGCID: Target Page

MyabA.01097.g

Putative oxidoreductase EphD

CENTER ID:	MyabA.01097.g
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01097.g.A1.GE30366	Full length( MyabA.01097.g )		1	306

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.1421
RefSeq:	YP_001702122.1
UniProt:	B1MLP1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAQGEDQVKR SDIKLVVVTG AGSGIGRAAA ERFAQLGARV IVSDINEVAA EATVAAIRDA GGDSCARRLD VTDVNEFEDF ARWVQSEHGV CDVVVNNAGI GILGEVIDTT ESDWDRIVGI NLLGMVHGAR LFAQQMVDAN KPGHIVNVAS AAAFLPMPNL ASYSATKYAV KMFTDCIRGE LAPNHIGASV ICPGPIMTNI FSSATHTGAD EQEQVQRSAL TGAAFEKIRS WHLIPGPQNV ARAIESAVVY NRKTVLVRPE AYLVYLLRRL SPTLLYAFSN LIFGSRTITF AYRLGKNPTV ARLLGR

NT Sequence

TTGGCTCAAG GAGAGGACCA AGTGAAGCGA AGTGACATCA AGCTGGTTGT GGTGACCGGA GCGGGCAGCG GTATAGGACG GGCCGCAGCT GAACGATTCG CACAGCTAGG CGCGCGGGTG ATCGTCAGTG ACATCAACGA GGTGGCGGCG GAGGCGACCG TCGCAGCGAT TCGGGATGCG GGAGGCGATT CGTGTGCCCG CCGTCTTGAC GTAACCGATG TAAACGAATT CGAGGATTTT GCGCGTTGGG TCCAGTCCGA ACACGGCGTA TGCGATGTCG TGGTCAATAA TGCGGGAATC GGCATTCTTG GTGAGGTCAT CGATACCACA GAATCGGACT GGGACCGCAT CGTCGGCATC AACCTGCTAG GCATGGTCCA TGGCGCACGT CTGTTCGCGC AACAGATGGT CGATGCGAAC AAGCCTGGCC ATATCGTAAA TGTCGCCTCA GCGGCAGCGT TTCTTCCGAT GCCGAACCTG GCGTCGTATT CGGCAACCAA GTACGCGGTC AAGATGTTCA CCGATTGCAT TCGCGGCGAA CTTGCTCCGA ATCATATTGG CGCCAGCGTT ATATGCCCTG GGCCAATCAT GACCAATATC TTCTCGTCCG CGACCCATAC CGGTGCCGAT GAGCAGGAGC AGGTGCAGCG CTCGGCGCTT ACTGGGGCGG CTTTCGAGAA GATTCGCAGC TGGCATCTGA TCCCCGGACC CCAGAATGTG GCGCGCGCTA TCGAGAGTGC GGTGGTGTAC AACCGCAAGA CCGTGTTGGT ACGGCCGGAA GCATATTTGG TTTACCTGTT GCGTCGGCTG TCTCCAACGC TGCTGTACGC CTTCTCGAAT CTTATCTTTG GTTCGCGCAC AATCACATTC GCATACCGGC TAGGTAAGAA TCCGACGGTG GCGCGACTGT TGGGCAGG

Details for MyabA.01097.g.A1.GE30366

HARVESTED ON:	9/8/2010
SEQUENCED ON:	9/17/2010
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	108

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLAQGEDQV KRSDIKLVVV TGAGSGIGRA AAERFAQLGA RVIVSDINEV AAEATVAAIR DAGGDSCARR LDVTDVNEFE DFARWVQSEH GVCDVVVNNA GIGILGEVID TTESDWDRIV GINLLGMVHG ARLFAQQMVD ANKPGHIVNV ASAAAFLPMP NLASYSATKY AVKMFTDCIR GELAPNHIGA SVICPGPIMT NIFSSATHTG ADEQEQVQRS ALTGAAFEKI RSWHLIPGPQ NVARAIESAV VYNRKTVLVR PEAYLVYLLR RLSPTLLYAF SNLIFGSRTI TFAYRLGKNP XXRDXWAGNK QHXSXAXXXL XXXXLTXXXX SXXXX

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgg ctcaaggaga ggaccaagtg aagcgaagtg acatcaagct ggttgtggtg accggagcgg gcagcggtat aggacgggcc gcagctgaac gattcgcaca gctaggcgcg cgggtgatcg tcagtgacat caacgaggtg gcggcggagg cgaccgtcgc agcgattcgg gatgcgggag gcgattcgtg tgcccgccgt cttgacgtaa ccgatgtaaa cgaattcgag gattttgcgc gttgggtcca gtccgaacac ggcgtatgcg atgtcgtggt caataatgcg ggaatcggca ttcttggtga ggtcatcgat accacagaat cggactggga ccgcatcgtc ggcatcaacc tgctaggcat ggtccatggc gcacgtctgt tcgcgcaaca gatggtcgat gcgaacaagc ctggccatat cgtaaatgtc gcctcagcgg cagcgtttct tccgatgccg aacctggcgt cgtattcggc aaccaagtac gcggtcaaga tgttcaccga ttgcattcgc ggcgaacttg ctccgaatca tattggcgcc agcgttatat gccctgggcc aatcatgacc aatatcttct cgtccgcgac ccataccggt gccgatgagc aggagcaggt gcagcgctcg gcgcttactg gggcggcttt cgagaagatt cgcagctggc atctgatccc cggaccccag aatgtggcgc gcgctatcga gagtgcggtg gtgtacaacc gcaagaccgt gttggtacgg ccggaagcat atttggttta cctgttgcgt cggctgtctc caacgctgct gtacgccttc tcgaatctta tctttggttc gcgcacaatc acattcgcat accggctagg taagaatccn annnggcgcg actgntgggc aggtaataaa cagcacnaaa gtnctgcanc cnnnntnctc nnngnnnngn ngctaacaaa nnncnaaann agcngantng nnnnngcc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggctcaa ggagaggacc aagtgaagcg aagtgacatc aagctggttg tggtgaccgg agcgggcagc ggtataggac gggccgcagc tgaacgattc gcacagctag gcgcgcgggt gatcgtcagt gacatcaacg aggtggcggc ggaggcgacc gtcgcagcga ttcgggatgc gggaggcgat tcgtgtgccc gccgtcttga cgtaaccgat gtaaacgaat tcgaggattt tgcgcgttgg gtccagtccg aacacggcgt atgcgatgtc gtggtcaata atgcgggaat cggcattctt ggtgaggtca tcgataccac agaatcggac tgggaccgca tcgtcggcat caacctgcta ggcatggtcc atggcgcacg tctgttcgcg caacagatgg tcgatgcgaa caagcctggc catatcgtaa atgtcgcctc agcggcagcg tttcttccga tgccgaacct ggcgtcgtat tcggcaacca agtacgcggt caagatgttc accgattgca ttcgcggcga acttgctccg aatcatattg gcgccagcgt tatatgccct gggccaatca tgaccaatat cttctcgtcc gcgacccata ccggtgccga tgagcaggag caggtgcagc gctcggcgct tactggggcg gctttcgaga agattcgcag ctggcatctg atccccggac cccagaatgt ggcgcgcgct atcgagagtg cggtggtgta caaccgcaag accgtgttgg tacggccgga agcatatttg gtttacctgt tgcgtcggct gtctccaacg ctgctgtacg ccttctcgaa tcttatcttt ggttcgcgca caatcacatt cgcataccgg ctaggtaaga atccgacggt ggcgcgactg ttgggcaggt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa