MyabA.01097.e

Putative short chain dehydrogenase/reductase

CENTER ID: MyabA.01097.e
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01097.e.A2.GE30364 Mature protein( MyabA.01097.e ) 27 287
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.3992
RefSeq: YP_001704647.1
UniProt: B1MHB2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNFDNKVAV ITGAGSGIGR SLALALAQRG ARLALSDIDT AGVADTAGRC EKAGATAIPF ELDVADRAAV YAHAADVKNE FGQVNLVFNN AGVALSADVK DMEWDDFDWL MNINFWGVAH GTKAFLPHLI ESGDGHIVNV SSVFGLMGIP SQSAYNAAKF AVRGFTESLR QEIRAAKFPV GVTCVHPGGI KTNIASSARG IPDGVDRETV RKGFQLMAIT RPDSAARIIL RGVEKNRPRV LIGPDARVFD AIPRIIGPRY EDLMAPFYGM GRYGIGKKIA GRFGIEL
NT Sequence
ATGAAGAACT TCGACAACAA GGTTGCCGTA ATCACCGGTG CCGGTTCAGG TATCGGCCGC AGCCTGGCGT TGGCGTTGGC GCAGCGTGGT GCGCGCTTGG CACTTTCGGA CATCGATACC GCCGGGGTGG CCGATACCGC CGGGCGCTGC GAGAAGGCGG GCGCGACCGC CATCCCCTTC GAGCTCGACG TGGCCGATCG CGCCGCCGTC TATGCCCATG CCGCCGACGT CAAGAATGAG TTCGGGCAGG TCAATCTGGT GTTCAACAAT GCCGGGGTGG CACTTTCGGC CGATGTCAAG GACATGGAAT GGGATGACTT CGACTGGTTG ATGAATATCA ACTTCTGGGG TGTGGCACAC GGCACCAAGG CATTCCTGCC ACACCTGATC GAATCGGGCG ACGGCCATAT CGTCAATGTG TCGTCGGTGT TCGGTTTGAT GGGCATACCC TCTCAAAGTG CCTACAACGC GGCCAAATTC GCAGTCCGCG GTTTCACCGA ATCGCTGCGC CAGGAGATTC GGGCGGCCAA GTTCCCGGTC GGTGTCACCT GTGTGCACCC CGGGGGCATC AAGACCAACA TCGCCTCCAG CGCGCGCGGT ATCCCCGACG GAGTGGATCG AGAGACCGTC CGCAAGGGGT TCCAGCTGAT GGCCATCACC CGGCCGGACT CGGCGGCACG GATCATTCTG CGGGGTGTCG AAAAGAACCG TCCACGGGTT CTCATCGGTC CCGACGCACG CGTGTTCGAT GCCATCCCGC GCATCATCGG GCCGCGCTAC GAGGACCTGA TGGCGCCCTT CTACGGAATG GGCAGGTACG GGATCGGTAA GAAGATCGCG GGCCGCTTCG GTATCGAGTT G
Details for MyabA.01097.e.A2.GE30364
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 30kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAQRGARLA LSDIDTAGVA DTAGRCEKAG ATAIPFELDV ADRAAVYAHA ADVKNEFGQV NLVFNNAGVA LSADVKDMEW DDFDWLMNIN FWGVAHGTKA FLPHLIESGD GHIVNVSSVF GLMGIPSQSA YNAAKFAVRG FTESLRQEIR AAKFPVGVTC VHPGGIKTNI ASSARGIPDG VDRETVRKGF QLMAITRPDS AARIILRGVE KNRPRVLIGP DARVFDAIPR IIGPRYEDLM APFYGMGRYG IGKKIAGRFX IEL
Validated NT Sequence
ttttntttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcgca gcgtggtgcg cgcttggcac tttcggacat cgataccgcc ggggtggccg ataccgccgg gcgctgcgag aaggcgggcg cgaccgccat ccccttcgag ctcgacgtgg ccgatcgcgc cgccgtctat gcccatgccg ccgacgtcaa gaatgagttc gggcaggtca atctggtgtt caacaatgcc ggggtggcac tttcggccga tgtcaaggac atggaatggg atgacttcga ctggttgatg aatatcaact tctggggtgt ggcacacggc accaaggcat tcctgccaca cctgatcgaa tcgggcgacg gccatatcgt caatgtgtcg tcggtgttcg gtttgatggg cataccctct caaagtgcct acaacgcggc caaattcgca gtccgcggtt tcaccgaatc gctgcgccag gagattcggg cggccaagtt cccggtcggt gtcacctgtg tgcaccccgg gggcatcaag accaacatcg cctccagcgc gcgcggtatc cccgacggag tggatcgaga gaccgtccgc aaggggttcc agctgatggc catcacccgg ccggactcgg cggcacggat cattctgcgg ggtgtcgaaa agaaccgtcc acgggttctc atcggtcccg acgcacgcgt gttcgatgcc atcccgcgca tcatcgggcc gcgctacgag gacctgatgg cgcccttcta cggaatgggc aggtacggga tcggtaagaa gatcgcgggc cgcttcngta tcgagttgta ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatncnggct gctaacaaag cccgaaagga agctgagttg gctgctgccn cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAQRGARLA LSDIDTAGVA DTAGRCEKAG ATAIPFELDV ADRAAVYAHA ADVKNEFGQV NLVFNNAGVA LSADVKDMEW DDFDWLMNIN FWGVAHGTKA FLPHLIESGD GHIVNVSSVF GLMGIPSQSA YNAAKFAVRG FTESLRQEIR AAKFPVGVTC VHPGGIKTNI ASSARGIPDG VDRETVRKGF QLMAITRPDS AARIILRGVE KNRPRVLIGP DARVFDAIPR IIGPRYEDLM APFYGMGRYG IGKKIAGRFG IEL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgcagcgt ggtgcgcgct tggcactttc ggacatcgat accgccgggg tggccgatac cgccgggcgc tgcgagaagg cgggcgcgac cgccatcccc ttcgagctcg acgtggccga tcgcgccgcc gtctatgccc atgccgccga cgtcaagaat gagttcgggc aggtcaatct ggtgttcaac aatgccgggg tggcactttc ggccgatgtc aaggacatgg aatgggatga cttcgactgg ttgatgaata tcaacttctg gggtgtggca cacggcacca aggcattcct gccacacctg atcgaatcgg gcgacggcca tatcgtcaat gtgtcgtcgg tgttcggttt gatgggcata ccctctcaaa gtgcctacaa cgcggccaaa ttcgcagtcc gcggtttcac cgaatcgctg cgccaggaga ttcgggcggc caagttcccg gtcggtgtca cctgtgtgca ccccgggggc atcaagacca acatcgcctc cagcgcgcgc ggtatccccg acggagtgga tcgagagacc gtccgcaagg ggttccagct gatggccatc acccggccgg actcggcggc acggatcatt ctgcggggtg tcgaaaagaa ccgtccacgg gttctcatcg gtcccgacgc acgcgtgttc gatgccatcc cgcgcatcat cgggccgcgc tacgaggacc tgatggcgcc cttctacgga atgggcaggt acgggatcgg taagaagatc gcgggccgct tcggtatcga gttgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa