SSGCID: Target Page

MyabA.01097.c

Putative oxidoreductase EphD

CENTER ID:	MyabA.01097.c
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01097.c.A1.GE30362	Full length( MyabA.01097.c )		1	295

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.1420
RefSeq:	YP_001702121.1
UniProt:	B1MLP0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNLRDQVVFI TGAGSGIGRA TALAFARGGA KVIATDRNLA SAEQTAELVH VSRGVCEPVL LDVADPLAWE SALARVEEHH GIPRVLVNNA GYTTAGRFLD HSADDWADLV AVNVRGAVTG SRLCARRMVD AGIRGQIINV ASGAAYTPLT VSSPYCTTKA AVMMFSEAFR LELRPHGIGV TAICPGAINT GFYTAARHLG SDAAVNTQRR DLSAGAIARF GSGPDAVAKA IVRSVRANRA IQPVTTEAYL GYALWRLSPT VMKVIARLSG EGTLRYVENT SLVRTAMGWL KERTK

NT Sequence

ATGAACCTGC GTGACCAAGT TGTTTTCATT ACCGGTGCGG GTAGTGGCAT CGGGCGTGCG ACCGCCCTTG CGTTCGCCCG GGGCGGTGCA AAAGTCATAG CCACGGACCG GAATTTGGCC TCCGCAGAGC AGACGGCAGA GCTGGTGCAT GTATCCCGTG GTGTGTGCGA GCCGGTCCTG TTGGATGTGG CCGACCCGCT GGCATGGGAG AGCGCCTTGG CGAGAGTCGA AGAGCACCAT GGTATTCCGC GCGTTCTAGT GAACAATGCC GGATACACGA CAGCGGGCCG CTTTTTGGAC CACTCTGCCG ATGACTGGGC AGACTTGGTT GCGGTCAACG TGCGCGGTGC GGTTACTGGG TCGCGTTTGT GTGCGCGCCG AATGGTCGAC GCCGGTATCC GCGGGCAGAT CATCAATGTG GCTTCGGGAG CCGCGTACAC GCCGTTGACC GTCAGCTCGC CGTATTGCAC GACAAAGGCA GCGGTAATGA TGTTCTCGGA AGCGTTCAGA TTGGAGCTAC GTCCGCACGG GATCGGTGTG ACCGCAATCT GTCCAGGTGC CATCAACACC GGTTTCTATA CCGCGGCTCG CCATCTGGGC TCCGACGCCG CCGTCAACAC TCAACGCCGG GATCTTTCCG CTGGGGCGAT AGCCCGGTTC GGAAGCGGGC CAGATGCGGT GGCCAAAGCC ATCGTGCGAT CGGTGCGCGC CAACCGCGCC ATCCAACCGG TTACCACCGA GGCATATCTG GGGTATGCAC TGTGGCGACT CAGCCCCACA GTGATGAAGG TGATCGCGCG GTTGAGCGGG GAAGGCACCC TGCGATACGT CGAAAACACG TCACTGGTGC GCACGGCCAT GGGTTGGCTC AAGGAGAGGA CCAAG

Details for MyabA.01097.c.A1.GE30362

HARVESTED ON:	9/8/2010
SEQUENCED ON:	9/17/2010
EXPECTED MW:	33kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	94
PERCENT COVERAGE:	106

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNLRDQVVF ITGAGSGIGR ATALAFARGG AKVIATDRNL ASAEQTAELV HVSRGVCEPV LLDVADPLAW ESALARVEEH HGIPRVLVNN AGYTTAGRFL DHSADDWADL VAVNVRGAVT GSRLCARRMV DAGIRGQIIN VASGAAYTPL TVSSPYCTTK AAVMMFSEAF RLELRPHGIG VTAICPGAIN TGFYTAARHL GSDAAVNTQR RDLSAGAIAR FGSGPDAVAK AIVRSVRANR AIQPVTTEAY LGYALWRLXP TVMXVIARLS GEXXPAIRXN TSLVRTXHGL AXXXXXXXST NKXCSXLXXX XANKXXXS

Validated NT Sequence

attttgtnnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaacc tgcgtgacca agttgttttc attaccggtg cgggtagtgg catcgggcgt gcgaccgccc ttgcgttcgc ccggggcggt gcaaaagtca tagccacgga ccggaatttg gcctccgcag agcagacggc agagctggtg catgtatccc gtggtgtgtg cgagccggtc ctgttggatg tggccgaccc gctggcatgg gagagcgcct tggcgagagt cgaagagcac catggtattc cgcgcgttct agtgaacaat gccggataca cgacagcggg ccgctttttg gaccactctg ccgatgactg ggcagacttg gttgcggtca acgtgcgcgg tgcggttact gggtcgcgtt tgtgtgcgcg ccgaatggtc gacgccggta tccgcgggca gatcatcaat gtggcttcgg gagccgcgta cacgccgttg accgtcagct cgccgtattg cacgacaaag gcagcggtaa tgatgttctc ggaagcgttc agattggagc tacgtccgca cgggatcggt gtgaccgcaa tctgtccagg tgccatcaac accggtttct ataccgcggc tcgccatctg ggctccgacg ccgccgtcaa cactcaacgc cgggatcttt ccgctggggc gatagcccgg ttcggaagcg ggccagatgc ggtggccaaa gccatcgtgc gatcggtgcg cgccaaccgc gccatccaac cggttaccac cgaggcatat ctggggtatg cactgtggcg actcnncccc acagtgatga angtgatcgc gcggttgagc ggggaanggn accctgcgat acgtcnaaac acgtcactgg tgcgcacngn ccatgggttg gctcnnnaga gnancnagtn nnacagcacg aacaagtnct gcagccnnct nncnannnnn nntgctaaca agcngaangn nagctgantn gctgctgccn nnnnnnnnnn annnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacctgcgt gaccaagttg ttttcattac cggtgcgggt agtggcatcg ggcgtgcgac cgcccttgcg ttcgcccggg gcggtgcaaa agtcatagcc acggaccgga atttggcctc cgcagagcag acggcagagc tggtgcatgt atcccgtggt gtgtgcgagc cggtcctgtt ggatgtggcc gacccgctgg catgggagag cgccttggcg agagtcgaag agcaccatgg tattccgcgc gttctagtga acaatgccgg atacacgaca gcgggccgct ttttggacca ctctgccgat gactgggcag acttggttgc ggtcaacgtg cgcggtgcgg ttactgggtc gcgtttgtgt gcgcgccgaa tggtcgacgc cggtatccgc gggcagatca tcaatgtggc ttcgggagcc gcgtacacgc cgttgaccgt cagctcgccg tattgcacga caaaggcagc ggtaatgatg ttctcggaag cgttcagatt ggagctacgt ccgcacggga tcggtgtgac cgcaatctgt ccaggtgcca tcaacaccgg tttctatacc gcggctcgcc atctgggctc cgacgccgcc gtcaacactc aacgccggga tctttccgct ggggcgatag cccggttcgg aagcgggcca gatgcggtgg ccaaagccat cgtgcgatcg gtgcgcgcca accgcgccat ccaaccggtt accaccgagg catatctggg gtatgcactg tggcgactca gccccacagt gatgaaggtg atcgcgcggt tgagcgggga aggcaccctg cgatacgtcg aaaacacgtc actggtgcgc acggccatgg gttggctcaa ggagaggacc aagtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa