SSGCID: Target Page

MyabA.01097.b

Probable oxidoreductase EphD

CENTER ID:	MyabA.01097.b
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.01097.b.A1.GE30361	Full length( MyabA.01097.b )		1	277

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.121
RefSeq:	YP_001700844.1
UniProt:	B1MEC6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSGSAQRNS LVVVTGAGSG IGRETSLAFA RQGARVVVAD INHDTANETV GLIEKLGGSA YPYALDVSDE AAVTAFADDV CAKHGVPDVL INNAGVGHGG RFFETSSTDF QRVLNINLGG VVHGCRAFGP RMAERKSGHI VNISSAAAYT PIPEMGAYAT SKAAVFMFSD VLRGELARDK VKVSTICPGI VNTNIIRTTQ FSGLSPEDEA KRQQQGAGLY AKRGYGPEKV AKEIVNAVAK GKSVVPVTPE AHVQYHFSRL APALNRFFWG LSDKISR

NT Sequence

ATGACATCCG GCAGTGCCCA GCGCAACAGC CTGGTGGTGG TGACCGGGGC CGGCAGCGGC ATCGGCCGCG AGACCTCGCT GGCCTTCGCC CGCCAGGGCG CCCGTGTCGT GGTCGCCGAT ATCAACCATG ACACCGCCAA CGAGACCGTC GGGCTCATCG AGAAGCTCGG CGGGTCCGCG TACCCGTACG CGCTGGACGT GTCCGACGAG GCGGCGGTGA CTGCCTTCGC CGACGATGTG TGCGCCAAGC ACGGCGTGCC CGATGTCCTG ATCAACAACG CCGGCGTCGG ACACGGCGGC CGGTTCTTCG AGACGTCTTC CACCGACTTC CAGCGGGTAC TCAACATCAA CCTGGGCGGA GTGGTCCACG GCTGTCGCGC GTTCGGGCCG CGGATGGCCG AGCGCAAGAG CGGCCACATC GTGAACATCT CCAGTGCCGC TGCATACACG CCGATCCCCG AGATGGGCGC CTACGCCACC AGCAAGGCGG CCGTGTTCAT GTTCTCCGAC GTGCTGCGCG GCGAACTCGC GCGCGACAAG GTGAAGGTGA GCACCATCTG CCCGGGCATC GTCAACACGA ACATCATTCG CACCACCCAG TTCTCGGGCC TCTCGCCGGA GGACGAGGCC AAGCGGCAAC AGCAGGGAGC GGGCCTGTAC GCCAAGCGTG GATACGGCCC GGAGAAGGTC GCCAAGGAAA TCGTCAACGC CGTCGCCAAG GGTAAGTCGG TTGTGCCGGT GACTCCGGAG GCACACGTGC AGTACCACTT CAGCCGCCTG GCCCCGGCGC TGAACAGGTT CTTCTGGGGC CTCAGCGACA AGATCAGTCG G

Details for MyabA.01097.b.A1.GE30361

HARVESTED ON:	9/8/2010
SEQUENCED ON:	9/17/2010
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTSGSAQRN SLVVVTGAGS GIGRETSLAF ARQGARVVVA DINHDTANET VGLIEKLGGS AYPYALDVSD EAAVTAFADD VCAKHGVPDV LINNAGVGHG GRFFETSSTD FQRVLNINLG GVVHGCRAFG PRMAERKSGH IVNISSAAAY TPIPEMGAYA TSKAAVFMFS DVLRGELARD KVKVSTICPG IVNTNIIRTT QFSGLSPEDE AKRQQQGAGL YAKRGYGPEK VAKEIVNAVA KGKSVVPVTP EAHVQYHFSR LAPALNRFFW GLSDKISR

Validated NT Sequence

ttattaccga ctgatcttgt cgctgaggcc ccagaagaac ctgttcagcg ccggggccag gcggctgaag tggtactgca cgtgtgcctc cggagtcacc ggcacaaccg acttaccctt ggcgacggcg ttgacgattt ccttggcgac cttctccggg ccgtatccac gcttggcgta caggcccgct ccctgctgtt gccgcttggc ctcgtcctcc ggcgagaggc ccgagaactg ggtggtgcga atgatgttcg tgttgacgat gcccgggcag atggtgctca ccttcacctt gtcgcgcgcg agttcgccgc gcagcacgtc ggagaacatg aacacggccg ccttgctggt ggcgtaggcg cccatctcgg ggatcggcgt gtatgcagcg gcactggaga tgttcacgat gtggccgctc ttgcgctcgg ccatccgcgg cccgaacgcg cgacagccgt ggaccactcc gcccaggttg atgttgagta cccgctggaa gtcggtggaa gacgtctcga agaaccggcc gccgtgtccg acgccggcgt tgttgatcag gacatcgggc acgccgtgct tggcgcacac atcgtcggcg aaggcagtca ccgccgcctc gtcggacacg tccagcgcgt acgggtacgc ggacccgccg agcttctcga tgagcccgac ggtctcgttg gcggtgtcat ggttgatatc ggcgaccacg acacgggcgc cctggcgggc gaaggccagc gaggtctcgc ggccgatgcc gctgccggcc ccggtcacca ccaccaggct gttgcgctgg gcactgccgg atgtcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacatccggc agtgcccagc gcaacagcct ggtggtggtg accggggccg gcagcggcat cggccgcgag acctcgctgg ccttcgcccg ccagggcgcc cgtgtcgtgg tcgccgatat caaccatgac accgccaacg agaccgtcgg gctcatcgag aagctcggcg ggtccgcgta cccgtacgcg ctggacgtgt ccgacgaggc ggcggtgact gccttcgccg acgatgtgtg cgccaagcac ggcgtgcccg atgtcctgat caacaacgcc ggcgtcggac acggcggccg gttcttcgag acgtcttcca ccgacttcca gcgggtactc aacatcaacc tgggcggagt ggtccacggc tgtcgcgcgt tcgggccgcg gatggccgag cgcaagagcg gccacatcgt gaacatctcc agtgccgctg catacacgcc gatccccgag atgggcgcct acgccaccag caaggcggcc gtgttcatgt tctccgacgt gctgcgcggc gaactcgcgc gcgacaaggt gaaggtgagc accatctgcc cgggcatcgt caacacgaac atcattcgca ccacccagtt ctcgggcctc tcgccggagg acgaggccaa gcggcaacag cagggagcgg gcctgtacgc caagcgtgga tacggcccgg agaaggtcgc caaggaaatc gtcaacgccg tcgccaaggg taagtcggtt gtgccggtga ctccggaggc acacgtgcag taccacttca gccgcctggc cccggcgctg aacaggttct tctggggcct cagcgacaag atcagtcggt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa