MyabA.01097.a

Putative short chain dehydrogenase/reductase

CENTER ID: MyabA.01097.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01097.a.A1.GE30360 Full length( MyabA.01097.a ) 1 269
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01097.a.A1.PW31642 Full length( MyabA.01097.a ) 1 269

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.3154
RefSeq: YP_001703834.1
UniProt: B1MD59

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDINGSVAIV TGAGSGIGQA LAWGLVDAGA TVVASDIDAD AVGRTAAARD GIVGQRADAS AVSDIEDLIA LAEKEFGPVD LYAANAGIAG GLGLGIGEAA WDLTIDVNLR AHIRAATLLV PGWVERGRGY FLSVASAAGL LTQIGSPAYS VTKHAAVGFA EWLSITYGGQ GVGVSCLCPM GVKTALLDGL TESDDPDVRV AGTSITAAGD VLEPAVVAAM ALDAIRDEKF LVLPHPQVLD MYRQKGADYD RWIAGMRRYQ AILEDQIIR
NT Sequence
ATGGATATCA ACGGCAGCGT CGCCATCGTC ACCGGAGCCG GTTCGGGAAT CGGACAGGCC CTTGCCTGGG GGCTTGTGGA TGCCGGAGCC ACCGTGGTGG CGTCAGATAT CGACGCCGAT GCCGTGGGGC GCACCGCCGC GGCTCGAGAC GGCATCGTCG GTCAGCGAGC CGATGCCAGC GCGGTCTCCG ATATCGAGGA TCTTATTGCT TTGGCGGAGA AGGAATTTGG TCCCGTCGAT CTCTACGCGG CCAACGCCGG TATCGCCGGC GGCCTCGGGC TCGGTATCGG CGAGGCGGCA TGGGACCTCA CCATCGATGT GAACCTTCGG GCGCATATCC GCGCGGCCAC GCTGCTGGTG CCCGGATGGG TAGAGCGCGG CCGTGGCTAT TTCCTGTCGG TGGCATCAGC GGCCGGACTG CTCACCCAGA TCGGTTCACC CGCGTATTCG GTGACCAAAC ATGCCGCGGT CGGCTTTGCC GAGTGGCTCT CGATCACCTA CGGCGGTCAG GGGGTCGGAG TGAGTTGCCT GTGTCCGATG GGGGTCAAGA CGGCACTCTT GGACGGCCTC ACCGAATCCG ATGACCCGGA CGTGCGTGTG GCGGGTACCT CTATAACAGC GGCGGGTGAC GTGCTCGAAC CGGCGGTGGT GGCTGCCATG GCACTCGATG CGATCCGGGA TGAGAAGTTC TTGGTCTTGC CGCATCCCCA GGTGCTCGAC ATGTATCGCC AGAAGGGCGC CGATTACGAC AGATGGATCG CGGGAATGCG CCGTTACCAG GCCATACTCG AAGACCAGAT CATCCGG
Details for MyabA.01097.a.A1.GE30360
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 96
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDINGSVAI VTGAGSGIGQ ALAWGLVDAG ATVVASDIDA DAVGRTAAAR DGIVGQRADA SAVSDIEDLI ALAEKEFGPV DLYAANAGIA GGLGLGIGEA AWDLTIDVNL RAHIRAATLL VPGWVERGRG YFLSVASAAG LLTQIGSPAY SVTKHAAVGF AEWLSITYGG QGVGVSCLCP MGVKTALLDG LTESDDPDVR VAGTSITAAG DVLEPAVVAA MALDAIRDEK FLVLPHPXVL DMYRQXXRRL XQMDRGNAPL
Validated NT Sequence
ggtaacggcg cattcccgcg atccatctgt ngtaatcggc gccnnttctg gcgatacatg tcgagcaccn ggggatgcgg caagaccaag aacttctcat cccggatcgc atcgagtgcc atggcagcca ccaccgccgg ttcgagcacg tcacccgccg ctgttataga ggtacccgcc acacgcacgt ccgggtcatc ggattcggtg aggccgtcca agagtgccgt cttgaccccc atcggacaca ggcaactcac tccgaccccc tgaccgccgt aggtgatcga gagccactcg gcaaagccga ccgcggcatg tttggtcacc gaatacgcgg gtgaaccgat ctgggtgagc agtccggccg ctgatgccac cgacaggaaa tagccacggc cgcgctctac ccatccgggc accagcagcg tggccgcgcg gatatgcgcc cgaaggttca catcgatggt gaggtcccat gccgcctcgc cgataccgag cccgaggccg ccggcgatac cggcgttggc cgcgtagaga tcgacgggac caaattcctt ctccgccaaa gcaataagat cctcgatatc ggagaccgcg ctggcatcgg ctcgctgacc gacgatgccg tctcgagccg cggcggtgcg ccccacggca tcggcgtcga tatctgacgc caccacggtg gctccggcat ccacaagccc ccaggcaagg gcctgtccga ttcccgaacc ggctccggtg acgatggcga cgctgccgtt gatatccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDINGSVAI VTGAGSGIGQ ALAWGLVDAG ATVVASDIDA DAVGRTAAAR DGIVGQRADA SAVSDIEDLI ALAEKEFGPV DLYAANAGIA GGLGLGIGEA AWDLTIDVNL RAHIRAATLL VPGWVERGRG YFLSVASAAG LLTQIGSPAY SVTKHAAVGF AEWLSITYGG QGVGVSCLCP MGVKTALLDG LTESDDPDVR VAGTSITAAG DVLEPAVVAA MALDAIRDEK FLVLPHPQVL DMYRQKGADY DRWIAGMRRY QAILEDQIIR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatatcaac ggcagcgtcg ccatcgtcac cggagccggt tcgggaatcg gacaggccct tgcctggggg cttgtggatg ccggagccac cgtggtggcg tcagatatcg acgccgatgc cgtggggcgc accgccgcgg ctcgagacgg catcgtcggt cagcgagccg atgccagcgc ggtctccgat atcgaggatc ttattgcttt ggcggagaag gaatttggtc ccgtcgatct ctacgcggcc aacgccggta tcgccggcgg cctcgggctc ggtatcggcg aggcggcatg ggacctcacc atcgatgtga accttcgggc gcatatccgc gcggccacgc tgctggtgcc cggatgggta gagcgcggcc gtggctattt cctgtcggtg gcatcagcgg ccggactgct cacccagatc ggttcacccg cgtattcggt gaccaaacat gccgcggtcg gctttgccga gtggctctcg atcacctacg gcggtcaggg ggtcggagtg agttgcctgt gtccgatggg ggtcaagacg gcactcttgg acggcctcac cgaatccgat gacccggacg tgcgtgtggc gggtacctct ataacagcgg cgggtgacgt gctcgaaccg gcggtggtgg ctgccatggc actcgatgcg atccgggatg agaagttctt ggtcttgccg catccccagg tgctcgacat gtatcgccag aagggcgccg attacgacag atggatcgcg ggaatgcgcc gttaccaggc catactcgaa gaccagatca tccggtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MyabA.01097.a.A1.PW31642
PURIFICATION DATe: 2/5/2011
CONCENTRATION: 37.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 50µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDINGSVAI VTGAGSGIGQ ALAWGLVDAG ATVVASDIDA DAVGRTAAAR DGIVGQRADA SAVSDIEDLI ALAEKEFGPV DLYAANAGIA GGLGLGIGEA AWDLTIDVNL RAHIRAATLL VPGWVERGRG YFLSVASAAG LLTQIGSPAY SVTKHAAVGF AEWLSITYGG QGVGVSCLCP MGVKTALLDG LTESDDPDVR VAGTSITAAG DVLEPAVVAA MALDAIRDEK FLVLPHPXVL DMYRQXXRRL XQMDRGNAPL
Validated NT Sequence
ggtaacggcg cattcccgcg atccatctgt ngtaatcggc gccnnttctg gcgatacatg tcgagcaccn ggggatgcgg caagaccaag aacttctcat cccggatcgc atcgagtgcc atggcagcca ccaccgccgg ttcgagcacg tcacccgccg ctgttataga ggtacccgcc acacgcacgt ccgggtcatc ggattcggtg aggccgtcca agagtgccgt cttgaccccc atcggacaca ggcaactcac tccgaccccc tgaccgccgt aggtgatcga gagccactcg gcaaagccga ccgcggcatg tttggtcacc gaatacgcgg gtgaaccgat ctgggtgagc agtccggccg ctgatgccac cgacaggaaa tagccacggc cgcgctctac ccatccgggc accagcagcg tggccgcgcg gatatgcgcc cgaaggttca catcgatggt gaggtcccat gccgcctcgc cgataccgag cccgaggccg ccggcgatac cggcgttggc cgcgtagaga tcgacgggac caaattcctt ctccgccaaa gcaataagat cctcgatatc ggagaccgcg ctggcatcgg ctcgctgacc gacgatgccg tctcgagccg cggcggtgcg ccccacggca tcggcgtcga tatctgacgc caccacggtg gctccggcat ccacaagccc ccaggcaagg gcctgtccga ttcccgaacc ggctccggtg acgatggcga cgctgccgtt gatatccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDINGSVAI VTGAGSGIGQ ALAWGLVDAG ATVVASDIDA DAVGRTAAAR DGIVGQRADA SAVSDIEDLI ALAEKEFGPV DLYAANAGIA GGLGLGIGEA AWDLTIDVNL RAHIRAATLL VPGWVERGRG YFLSVASAAG LLTQIGSPAY SVTKHAAVGF AEWLSITYGG QGVGVSCLCP MGVKTALLDG LTESDDPDVR VAGTSITAAG DVLEPAVVAA MALDAIRDEK FLVLPHPQVL DMYRQKGADY DRWIAGMRRY QAILEDQIIR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATATCAAC GGCAGCGTCG CCATCGTCAC CGGAGCCGGT TCGGGAATCG GACAGGCCCT TGCCTGGGGG CTTGTGGATG CCGGAGCCAC CGTGGTGGCG TCAGATATCG ACGCCGATGC CGTGGGGCGC ACCGCCGCGG CTCGAGACGG CATCGTCGGT CAGCGAGCCG ATGCCAGCGC GGTCTCCGAT ATCGAGGATC TTATTGCTTT GGCGGAGAAG GAATTTGGTC CCGTCGATCT CTACGCGGCC AACGCCGGTA TCGCCGGCGG CCTCGGGCTC GGTATCGGCG AGGCGGCATG GGACCTCACC ATCGATGTGA ACCTTCGGGC GCATATCCGC GCGGCCACGC TGCTGGTGCC CGGATGGGTA GAGCGCGGCC GTGGCTATTT CCTGTCGGTG GCATCAGCGG CCGGACTGCT CACCCAGATC GGTTCACCCG CGTATTCGGT GACCAAACAT GCCGCGGTCG GCTTTGCCGA GTGGCTCTCG ATCACCTACG GCGGTCAGGG GGTCGGAGTG AGTTGCCTGT GTCCGATGGG GGTCAAGACG GCACTCTTGG ACGGCCTCAC CGAATCCGAT GACCCGGACG TGCGTGTGGC GGGTACCTCT ATAACAGCGG CGGGTGACGT GCTCGAACCG GCGGTGGTGG CTGCCATGGC ACTCGATGCG ATCCGGGATG AGAAGTTCTT GGTCTTGCCG CATCCCCAGG TGCTCGACAT GTATCGCCAG AAGGGCGCCG ATTACGACAG ATGGATCGCG GGAATGCGCC GTTACCAGGC CATACTCGAA GACCAGATCA TCCGGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA