MyabA.01062.a
Dihydrofolate reductase (DHFR)
CENTER ID:
MyabA.01062.a
ORGANISM:
Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:
Skin abscess
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
2
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
IIIC
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
MyabA.01062.a.B1.GE42259
full length
info
1
163
MyabA.01062.a.B1.GE42259
MyabA.01062.a.B11.GE42655
full length
info
1
163
MyabA.01062.a.B11.GE42655
MyabA.01062.a.B110.GE43448
full length
info
1
163
MyabA.01062.a.B110.GE43448
MyabA.01062.a.B111.GE43449
full length
info
1
163
MyabA.01062.a.B111.GE43449
MyabA.01062.a.B12.GE42656
full length
info
1
163
MyabA.01062.a.B12.GE42656
MyabA.01062.a.B14.GE43311
full length
info
1
163
MyabA.01062.a.B14.GE43311
MyabA.01062.a.B15.GE43312
full length
info
1
163
MyabA.01062.a.B15.GE43312
MyabA.01062.a.B16.GE43313
full length
info
1
163
MyabA.01062.a.B16.GE43313
MyabA.01062.a.B17.GE43314
full length
info
1
161
MyabA.01062.a.B17.GE43314
MyabA.01062.a.B18.GE43450
full length
info
1
161
MyabA.01062.a.B18.GE43450
MyabA.01062.a.B19.GE43451
full length
info
1
163
MyabA.01062.a.B19.GE43451
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
MyabA.01062.a.B11.PS38522
full length
info
1
163
PS38522
MyabA.01062.a.B12.PS38527
full length
info
1
163
PS38527
MyabA.01062.a.B14.PS38593
full length
info
1
163
PS38593
MyabA.01062.a.B17.PS38598
full length
info
1
161
PS38598
MyabA.01062.a.B110.PS38611
full length
info
1
163
PS38611
MyabA.01062.a.B111.PS38612
full length
info
1
163
PS38612
MyabA.01062.a.B1.PW38449
full length
info
1
163
PW38449
Structures
7K6C
DEPOSITED: 9/19/2020
DETERMINATION: XRay
CLONE: MyabA.01062.a.B15.GE43312
PROTEIN: MyabA.01062.a.B15.PS38597
External Resources
RESOURCE
REFERENCE ID
UniProt:
B1MD46
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGTIGLIWA QTRAGVIGAD GAIPWRLPED QARFKRITMG HTVIMGRKTW ESLPGSVRPL PGRPNIVLTR DALFEPDGAL AVGCADAALA ASDEAPWVIG GGEIYRLFLP LAQRCEVTVV EADVPGDALA PELGEGWVVE ENDWQTSESG LRYQFLSYRK VDG
NT Sequence
atgacgggaa ccatcgggct gatctgggcg caaacccgag ccggcgtcat cggcgcggac ggtgccattc cctggcggct acccgaagac caggcacgtt tcaagcgaat caccatgggg cacacagtga tcatggggcg caagacctgg gagtcgctgc ccggtagtgt ccgtcctttg ccgggtcggc ccaatatcgt gctgaccagg gatgccctgt tcgagcccga cggggcattg gccgtcggct gcgcggacgc ggccctggcg gcttccgacg aggcgccctg ggtcatcggc ggcggagaga tctatcggct gtttctgccg ttggcacaac gctgcgaagt gacggtggtg gaggccgacg tgccgggtga cgcgcttgct ccggaactcg gagaaggctg ggttgtcgag gaaaatgact ggcagacaag cgagtccggc ttgcgctatc aattcctgag ttatcggaaa gtcgacgggt ga
Details for MyabA.01062.a.B1.GE42259
HARVESTED ON:
2/23/2018
SEQUENCED ON:
3/1/2018
EXPECTED MW:
19kDa
OBSERVED MW:
19kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
Low Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B11.GE42655
HARVESTED ON:
9/26/2018
SEQUENCED ON:
9/26/2018
EXPECTED MW:
19kDa
OBSERVED MW:
20kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Many (50-100)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
Rosetta 2(DE3) Singles(TM) Competent Cells
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for MyabA.01062.a.B110.GE43448
HARVESTED ON:
9/6/2019
SEQUENCED ON:
8/20/2019
EXPECTED MW:
19kDa
OBSERVED MW:
21kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B111.GE43449
HARVESTED ON:
9/6/2019
SEQUENCED ON:
8/20/2019
EXPECTED MW:
19kDa
OBSERVED MW:
21kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B12.GE42656
HARVESTED ON:
9/27/2018
SEQUENCED ON:
9/26/2018
EXPECTED MW:
19kDa
OBSERVED MW:
20kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Many (50-100)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
Rosetta 2(DE3) Singles(TM) Competent Cells
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for MyabA.01062.a.B14.GE43311
HARVESTED ON:
7/18/2019
SEQUENCED ON:
6/21/2019
EXPECTED MW:
19kDa
OBSERVED MW:
19kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B15.GE43312
HARVESTED ON:
7/18/2019
SEQUENCED ON:
6/21/2019
EXPECTED MW:
19kDa
OBSERVED MW:
19kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B16.GE43313
HARVESTED ON:
7/18/2019
SEQUENCED ON:
6/21/2019
EXPECTED MW:
19kDa
OBSERVED MW:
19kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY:
99
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B17.GE43314
HARVESTED ON:
7/18/2019
SEQUENCED ON:
6/22/2019
EXPECTED MW:
18kDa
OBSERVED MW:
19kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Good (10-50)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B18.GE43450
HARVESTED ON:
9/6/2019
SEQUENCED ON:
8/2/2019
EXPECTED MW:
18kDa
OBSERVED MW:
21kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B19.GE43451
HARVESTED ON:
9/6/2019
SEQUENCED ON:
7/19/2019
EXPECTED MW:
19kDa
OBSERVED MW:
21kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Moderate Expression
SOLUBLE EXPRESSION LEVEL
Low Expression
EXPRESSION HOST:
Rosetta2 (DE3)
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B11.PS38522
PURIFICATION DATe:
10/23/2018
CONCENTRATION:
1.62mg/ml
OBSERVED MW:
18kDa
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:
Rosetta 2(DE3) Singles(TM) Competent Cells
VIAL COUNT (approx.):
5
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for MyabA.01062.a.B12.PS38527
PURIFICATION DATe:
11/6/2018
CONCENTRATION:
3.87mg/ml
OBSERVED MW:
20kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:
Rosetta 2(DE3) Singles(TM) Competent Cells
VIAL COUNT (approx.):
3
VIAL VOLUME:
110µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for MyabA.01062.a.B14.PS38593
PURIFICATION DATe:
8/13/2019
CONCENTRATION:
11.49mg/ml
OBSERVED MW:
19kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:
Rosetta2 (DE3)
VIAL COUNT (approx.):
3
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B17.PS38598
PURIFICATION DATe:
9/4/2019
CONCENTRATION:
15.03mg/ml
OBSERVED MW:
19kDa
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,
5% glycerol, 1 mM TCEP
EXPRESSION HOST:
Rosetta2 (DE3)
VIAL COUNT (approx.):
8
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B110.PS38611
PURIFICATION DATe:
10/16/2019
CONCENTRATION:
14.27mg/ml
OBSERVED MW:
19kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:
Rosetta2 (DE3)
VIAL COUNT (approx.):
7
VIAL VOLUME:
110µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B111.PS38612
PURIFICATION DATe:
10/15/2019
CONCENTRATION:
17.44mg/ml
OBSERVED MW:
19kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:
Rosetta2 (DE3)
VIAL COUNT (approx.):
3
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100
Details for MyabA.01062.a.B1.PW38449
PURIFICATION DATe:
4/2/2018
CONCENTRATION:
13.32mg/ml
OBSERVED MW:
20kDa
EXPRESSION LEVEL:
Moderate Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:
BL 21 (DE3) Rosetta
VIAL COUNT (approx.):
4
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
100