MyabA.01019.a

Dihydropteroate synthase (DHPS)

CENTER ID: MyabA.01019.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01019.a.B1.GE43494 full length 1 350
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01019.a.B1.PW38786 full length 1 350

External Resources

RESOURCE REFERENCE ID
UniProt: B1MGV0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIAGVTETPA LTHILGVVNV TDDSFSDGGR FVRQSDAVAH GLALASAGAD IIDVGGQSTR PGAAPIRQKI EMQRVIPVIK ELASHGINIS VDTMRAEVAA AAIDAGANMV NDVSGGRSDP EMAQLIAQRG VPWILMHWRS KDFIHTPATR NYRDVVADVS RELMESVAVA VNAGVSPDKL ILDPGLGFAK TAHHNWLLLQ AIPDLQELGY PILIGASRKR FLGSLLTDLK GVERPPDGRE TATAVITALG ALHEVWGVRV HDVTASMDAL KVVRAWETGG AETVEEGEEE AQEAAAPEPP PAPESPPVTI AQDEVTTEVT VRTARPEPSK TAGGRWRREV AQRGAKGEPK
NT Sequence
atgatcgctg gcgtgacgga aactcccgca ctgacccaca tcctgggagt cgtcaatgtc accgacgact ccttctctga cggtggacga ttcgtccgtc aatcggacgc ggtagcgcac ggcctggcgc ttgcgtccgc gggcgcggac atcatcgatg tcggtggcca gtcgactcgg cctggtgcgg cgccgatcag gcagaagatc gagatgcaac gtgtcatccc ggtgatcaaa gagcttgcca gtcatggcat caacatcagt gtggacacca tgcgtgcgga ggtcgcggcc gccgcgatcg atgccggcgc gaacatggtc aacgatgtct cgggcggcag gtccgacccg gagatggcgc agctgatcgc gcaacgaggt gttccttgga ttctgatgca ttggcgttca aaggatttca tccacacacc ggctacccga aactatcgcg atgtggtcgc cgacgtttct cgtgagctga tggagtcggt ggcggtggcg gtcaacgcgg gtgtctcacc ggacaagctc atcctcgacc cgggcctggg cttcgccaaa accgcacacc acaactggct gctgctgcaa gcgatcccgg acctgcaaga actgggctac ccgatcttga tcggtgcgtc ccgcaaacgg ttcctgggct cgctgcttac cgacctcaag ggtgtggagc ggccgcccga cggccgtgaa accgcgacgg ccgtcatcac cgcgctgggt gcgctgcacg aggtgtgggg ggtacgtgtc catgacgtga cggcctcgat ggacgcgttg aaggtggtgc gcgcctggga gaccggcggt gcggaaaccg tcgaagaggg tgaagaagaa gcccaagagg cagccgcccc ggaaccgccg cccgcgccgg aatccccgcc cgtgaccatc gcgcaggatg aggtcaccac cgaggtgacg gtgcgcaccg cacggcccga gcccagtaag accgcgggtg gcaggtggcg tcgggaggtg gcgcaacgcg gcgccaaagg tgaaccgaaa tga
Details for MyabA.01019.a.B1.GE43494
HARVESTED ON: 11/19/2019
SEQUENCED ON: 12/3/2019
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMI AGVTETPALT HILGVVNVTD DSFSDGGRFV RQSDAVAHGL ALASAGADII DVGGQSTRPG AAPIRQKIEM QRVIPVIKEL ASHGINISVD TMRAEVAAAA IDAGANMVND VSGGRSDPEM AQLIAQRGVP WILMHWRSKD FIHTPATRNY RDVVADVSRE LMESVAVAVN AGVSPDKLIL DPGLGFAKTA HHNWLLLQAI PDLQELGYPI LIGASRKRFL GSLLTDLKGV ERPPDGRETA TAVITALGAL HEVWGVRVHD VTASMDALKV VRAWETGGAE TVEEGEEEAQ EAAAPEPPPA PESPPVTIAQ DEVTTEVTVR TARPEPSKTA GGRWRREVAQ RGAKGEP
Validated NT Sequence
tttcggttca cctttggcgc cgcgttgcgc cacctcccga cgccacctgc cacccgcggt cttactgggc tcgggccgtg cggtgcgcac cgtcacctcg gtggtgacct catcctgcgc gatggtcacg ggcggggatt ccggcgcggg cggcggttcc ggggcggctg cctcttgggc ttcttcttca ccctcttcga cggtttccgc accgccggtc tcccaggcgc gcaccacctt caacgcgtcc atcgaggccg tcacgtcatg gacacgtacc ccccacacct cgtgcagcgc acccagcgcg gtgatgacgg ccgtcgcggt ttcacggccg tcgggcggcc gctccacacc cttgaggtcg gtaagcagcg agcccaggaa ccgtttgcgg gacgcaccga tcaagatcgg gtagcccagt tcttgcaggt ccgggatcgc ttgcagcagc agccagttgt ggtgtgcggt tttggcgaag cccaggcccg ggtcgaggat gagcttgtcc ggtgagacac ccgcgttgac cgccaccgcc accgactcca tcagctcacg agaaacgtcg gcgaccacat cgcgatagtt tcgggtagcc ggtgtgtgga tgaaatcctt tgaacgccaa tgcatcagaa tccaaggaac acctcgttgc gcgatcagct gcgccatctc cgggtcggac ctgccgcccg agacatcgtt gaccatgttc gcgccggcat cgatcgcggc ggccgcgacc tccgcacgca tggtgtccac actgatgttg atgccatgac tggcaagctc tttgatcacc gggatgacac gttgcatctc gatcttctgc ctgatcggcg ccgcaccagg ccgagtcgac tggccaccga catcgatgat gtccgcgccc gcggacgcaa gcgccaggcc gtgcgctacc gcgtccgatt gacggacgaa tcgtccaccg tcagagaagg agtcgtcggt gacattgacg actcccagga tgtgggtcag tgcgggagtt tccgtcacgc cagcgatcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMI AGVTETPALT HILGVVNVTD DSFSDGGRFV RQSDAVAHGL ALASAGADII DVGGQSTRPG AAPIRQKIEM QRVIPVIKEL ASHGINISVD TMRAEVAAAA IDAGANMVND VSGGRSDPEM AQLIAQRGVP WILMHWRSKD FIHTPATRNY RDVVADVSRE LMESVAVAVN AGVSPDKLIL DPGLGFAKTA HHNWLLLQAI PDLQELGYPI LIGASRKRFL GSLLTDLKGV ERPPDGRETA TAVITALGAL HEVWGVRVHD VTASMDALKV VRAWETGGAE TVEEGEEEAQ EAAAPEPPPA PESPPVTIAQ DEVTTEVTVR TARPEPSKTA GGRWRREVAQ RGAKGEPK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcg ctggcgtgac ggaaactccc gcactgaccc acatcctggg agtcgtcaat gtcaccgacg actccttctc tgacggtgga cgattcgtcc gtcaatcgga cgcggtagcg cacggcctgg cgcttgcgtc cgcgggcgcg gacatcatcg atgtcggtgg ccagtcgact cggcctggtg cggcgccgat caggcagaag atcgagatgc aacgtgtcat cccggtgatc aaagagcttg ccagtcatgg catcaacatc agtgtggaca ccatgcgtgc ggaggtcgcg gccgccgcga tcgatgccgg cgcgaacatg gtcaacgatg tctcgggcgg caggtccgac ccggagatgg cgcagctgat cgcgcaacga ggtgttcctt ggattctgat gcattggcgt tcaaaggatt tcatccacac accggctacc cgaaactatc gcgatgtggt cgccgacgtt tctcgtgagc tgatggagtc ggtggcggtg gcggtcaacg cgggtgtctc accggacaag ctcatcctcg acccgggcct gggcttcgcc aaaaccgcac accacaactg gctgctgctg caagcgatcc cggacctgca agaactgggc tacccgatct tgatcggtgc gtcccgcaaa cggttcctgg gctcgctgct taccgacctc aagggtgtgg agcggccgcc cgacggccgt gaaaccgcga cggccgtcat caccgcgctg ggtgcgctgc acgaggtgtg gggggtacgt gtccatgacg tgacggcctc gatggacgcg ttgaaggtgg tgcgcgcctg ggagaccggc ggtgcggaaa ccgtcgaaga gggtgaagaa gaagcccaag aggcagccgc cccggaaccg ccgcccgcgc cggaatcccc gcccgtgacc atcgcgcagg atgaggtcac caccgaggtg acggtgcgca ccgcacggcc cgagcccagt aagaccgcgg gtggcaggtg gcgtcgggag gtggcgcaac gcggcgccaa aggtgaaccg aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MyabA.01019.a.B1.PW38786
PURIFICATION DATe: 1/16/2020
CONCENTRATION: 17.6mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI AGVTETPALT HILGVVNVTD DSFSDGGRFV RQSDAVAHGL ALASAGADII DVGGQSTRPG AAPIRQKIEM QRVIPVIKEL ASHGINISVD TMRAEVAAAA IDAGANMVND VSGGRSDPEM AQLIAQRGVP WILMHWRSKD FIHTPATRNY RDVVADVSRE LMESVAVAVN AGVSPDKLIL DPGLGFAKTA HHNWLLLQAI PDLQELGYPI LIGASRKRFL GSLLTDLKGV ERPPDGRETA TAVITALGAL HEVWGVRVHD VTASMDALKV VRAWETGGAE TVEEGEEEAQ EAAAPEPPPA PESPPVTIAQ DEVTTEVTVR TARPEPSKTA GGRWRREVAQ RGAKGEP
Validated NT Sequence
tttcggttca cctttggcgc cgcgttgcgc cacctcccga cgccacctgc cacccgcggt cttactgggc tcgggccgtg cggtgcgcac cgtcacctcg gtggtgacct catcctgcgc gatggtcacg ggcggggatt ccggcgcggg cggcggttcc ggggcggctg cctcttgggc ttcttcttca ccctcttcga cggtttccgc accgccggtc tcccaggcgc gcaccacctt caacgcgtcc atcgaggccg tcacgtcatg gacacgtacc ccccacacct cgtgcagcgc acccagcgcg gtgatgacgg ccgtcgcggt ttcacggccg tcgggcggcc gctccacacc cttgaggtcg gtaagcagcg agcccaggaa ccgtttgcgg gacgcaccga tcaagatcgg gtagcccagt tcttgcaggt ccgggatcgc ttgcagcagc agccagttgt ggtgtgcggt tttggcgaag cccaggcccg ggtcgaggat gagcttgtcc ggtgagacac ccgcgttgac cgccaccgcc accgactcca tcagctcacg agaaacgtcg gcgaccacat cgcgatagtt tcgggtagcc ggtgtgtgga tgaaatcctt tgaacgccaa tgcatcagaa tccaaggaac acctcgttgc gcgatcagct gcgccatctc cgggtcggac ctgccgcccg agacatcgtt gaccatgttc gcgccggcat cgatcgcggc ggccgcgacc tccgcacgca tggtgtccac actgatgttg atgccatgac tggcaagctc tttgatcacc gggatgacac gttgcatctc gatcttctgc ctgatcggcg ccgcaccagg ccgagtcgac tggccaccga catcgatgat gtccgcgccc gcggacgcaa gcgccaggcc gtgcgctacc gcgtccgatt gacggacgaa tcgtccaccg tcagagaagg agtcgtcggt gacattgacg actcccagga tgtgggtcag tgcgggagtt tccgtcacgc cagcgatcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMI AGVTETPALT HILGVVNVTD DSFSDGGRFV RQSDAVAHGL ALASAGADII DVGGQSTRPG AAPIRQKIEM QRVIPVIKEL ASHGINISVD TMRAEVAAAA IDAGANMVND VSGGRSDPEM AQLIAQRGVP WILMHWRSKD FIHTPATRNY RDVVADVSRE LMESVAVAVN AGVSPDKLIL DPGLGFAKTA HHNWLLLQAI PDLQELGYPI LIGASRKRFL GSLLTDLKGV ERPPDGRETA TAVITALGAL HEVWGVRVHD VTASMDALKV VRAWETGGAE TVEEGEEEAQ EAAAPEPPPA PESPPVTIAQ DEVTTEVTVR TARPEPSKTA GGRWRREVAQ RGAKGEPK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCG CTGGCGTGAC GGAAACTCCC GCACTGACCC ACATCCTGGG AGTCGTCAAT GTCACCGACG ACTCCTTCTC TGACGGTGGA CGATTCGTCC GTCAATCGGA CGCGGTAGCG CACGGCCTGG CGCTTGCGTC CGCGGGCGCG GACATCATCG ATGTCGGTGG CCAGTCGACT CGGCCTGGTG CGGCGCCGAT CAGGCAGAAG ATCGAGATGC AACGTGTCAT CCCGGTGATC AAAGAGCTTG CCAGTCATGG CATCAACATC AGTGTGGACA CCATGCGTGC GGAGGTCGCG GCCGCCGCGA TCGATGCCGG CGCGAACATG GTCAACGATG TCTCGGGCGG CAGGTCCGAC CCGGAGATGG CGCAGCTGAT CGCGCAACGA GGTGTTCCTT GGATTCTGAT GCATTGGCGT TCAAAGGATT TCATCCACAC ACCGGCTACC CGAAACTATC GCGATGTGGT CGCCGACGTT TCTCGTGAGC TGATGGAGTC GGTGGCGGTG GCGGTCAACG CGGGTGTCTC ACCGGACAAG CTCATCCTCG ACCCGGGCCT GGGCTTCGCC AAAACCGCAC ACCACAACTG GCTGCTGCTG CAAGCGATCC CGGACCTGCA AGAACTGGGC TACCCGATCT TGATCGGTGC GTCCCGCAAA CGGTTCCTGG GCTCGCTGCT TACCGACCTC AAGGGTGTGG AGCGGCCGCC CGACGGCCGT GAAACCGCGA CGGCCGTCAT CACCGCGCTG GGTGCGCTGC ACGAGGTGTG GGGGGTACGT GTCCATGACG TGACGGCCTC GATGGACGCG TTGAAGGTGG TGCGCGCCTG GGAGACCGGC GGTGCGGAAA CCGTCGAAGA GGGTGAAGAA GAAGCCCAAG AGGCAGCCGC CCCGGAACCG CCGCCCGCGC CGGAATCCCC GCCCGTGACC ATCGCGCAGG ATGAGGTCAC CACCGAGGTG ACGGTGCGCA CCGCACGGCC CGAGCCCAGT AAGACCGCGG GTGGCAGGTG GCGTCGGGAG GTGGCGCAAC GCGGCGCCAA AGGTGAACCG AAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT