MyabA.00951.d

Putative amidase AmiA2

CENTER ID: MyabA.00951.d
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00951.d.A1.GE30381 Full length( MyabA.00951.d ) 1 466
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1719
RefSeq: YP_001702413.1
UniProt: B1MN47

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGFPTLTSQ AAALSDGSES SVSLTLTALN AIKSSQSTLN AFRVVCTKTA LAEATEADKR LARGERLPLL GVPIAIKDDT DLAGTPTAFG TAGAVGSESE DAELVRRLRA AGAVIVGKTN TCELGQWGFT SGPGFGHTRN PWSRGHTPGG SSGGSAAAVA AGLVAGAIGS DGAGSVRIPA AWTHLIGIKP QRGRISTWPF AEAFNGITVH GPLARTVADA ALLLDAASGN VPGDLHKPPH VRVLDAVHED PGQLRVALSL KVPFSGFPAQ LHPTIEAATR YVAHQLRSLG HMVSEGDPDY GVGLGLNFLP RATAGLLPWR DRLDAGAHWD PRTVTNMRTG RRLAGWALRR ARAAEPRLQA RVGKIFGSFN VVLAPTTAQP PTGIYDFDDV GSLATDRGQV GACPMTWPWN VLGWPSINVP AGFTADGLPI GVQLMGPANS EPLLISLAAQ LESINNWASE IPDPWW
NT Sequence
ATGTCGGGCT TTCCCACGCT GACTTCCCAG GCCGCAGCGC TATCTGACGG TTCGGAGAGT TCGGTCTCGC TCACCCTCAC GGCGCTCAAC GCGATCAAGT CGAGCCAGTC GACCCTCAAC GCATTCCGGG TGGTGTGCAC CAAGACGGCC CTGGCCGAAG CCACCGAGGC CGACAAGCGA TTAGCCAGGG GTGAGCGCCT GCCGTTGCTC GGAGTGCCCA TCGCCATCAA GGACGACACC GACTTGGCGG GCACTCCCAC CGCATTCGGT ACCGCCGGGG CCGTCGGCTC GGAGTCCGAG GACGCCGAGC TGGTGCGCCG ATTGCGCGCG GCGGGCGCGG TGATCGTCGG CAAGACCAAC ACCTGCGAGC TGGGCCAGTG GGGATTCACC AGCGGGCCCG GCTTCGGGCA CACCCGCAAC CCCTGGAGCC GCGGCCACAC CCCGGGCGGA TCCTCAGGTG GAAGCGCCGC GGCGGTGGCG GCCGGGCTCG TCGCCGGCGC CATCGGTTCG GACGGGGCCG GAAGCGTCCG GATCCCGGCG GCCTGGACGC ACCTGATCGG TATCAAGCCG CAACGCGGCC GCATCTCCAC CTGGCCGTTT GCCGAGGCCT TCAACGGGAT AACGGTGCAC GGACCGTTGG CCCGCACGGT CGCCGATGCC GCACTGCTGC TGGACGCGGC ATCGGGAAAC GTCCCCGGGG ACTTGCACAA GCCGCCGCAC GTCCGGGTGC TCGACGCCGT CCACGAGGAC CCGGGACAAC TGCGCGTCGC GTTATCGCTC AAGGTTCCGT TCTCGGGTTT CCCGGCGCAA CTGCATCCGA CCATCGAGGC CGCCACCCGC TATGTGGCCC ACCAGCTGCG TTCACTGGGG CACATGGTGT CCGAGGGCGA CCCGGACTAT GGCGTGGGGC TCGGCCTGAA CTTTCTGCCG CGAGCGACGG CCGGGCTGCT GCCGTGGCGC GATCGGCTGG ACGCCGGAGC GCATTGGGAC CCGCGCACGG TGACGAATAT GCGCACCGGC CGGCGCCTAG CCGGCTGGGC GCTGCGGCGA GCCCGCGCCG CCGAGCCGCG GCTGCAGGCC CGAGTGGGCA AGATCTTCGG CAGCTTCAAT GTGGTGTTGG CACCGACAAC CGCCCAACCG CCCACGGGCA TTTACGATTT CGATGATGTC GGCAGCCTCG CCACGGATCG CGGGCAGGTC GGCGCGTGTC CGATGACGTG GCCATGGAAC GTGCTGGGCT GGCCGTCGAT CAATGTGCCC GCCGGCTTCA CGGCCGACGG GCTGCCGATC GGTGTGCAGT TGATGGGCCC GGCGAACAGC GAGCCGCTGC TGATTTCCCT TGCCGCGCAG CTGGAGTCGA TCAACAATTG GGCGTCGGAG ATCCCCGATC CCTGGTGG
Details for MyabA.00951.d.A1.GE30381
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 50kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 80
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSGFPTLTS QAAALSDGSE SSVSLTLTAL NAIKSSQSTL NAFRVVCTKT ALAEATEADK RLARGERLPL LGVPIAIKDD TDLAGTPTAF GTAGAVGSES EDAELVRRLR AAGAVIVGKT NTCELGQWGF TSGPGFGHTR NPWSRGHTPG GSSGGSAAAV AAGLVAGAIG SDGAGSVRIP AAWTHLIGIK PQRGRISTWP FAEAFNGITV HGPLARTVAD AALLLDAASG NVPGXLHKPP HVRVLDAVHX DPGQLRVALS LKVPFSGFPA QLHPTIEAAT RYVAHQLRSL GHMXSXGDXX YGVGLGPXLS XASXXRGXXR GAIXLXXXXA XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
ttgtnnnntt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgtcgggct ttcccacgct gacttcccag gccgcagcgc tatctgacgg ttcggagagt tcggtctcgc tcaccctcac ggcgctcaac gcgatcaagt cgagccagtc gaccctcaac gcattccggg tggtgtgcac caagacggcc ctggccgaag ccaccgaggc cgacaagcga ttagccaggg gtgagcgcct gccgttgctc ggagtgccca tcgccatcaa ggacgacacc gacttggcgg gcactcccac cgcattcggt accgccgggg ccgtcggctc ggagtccgag gacgccgagc tggtgcgccg attgcgcgcg gcgggcgcgg tgatcgtcgg caagaccaac acctgcgagc tgggccagtg gggattcacc agcgggcccg gcttcgggca cacccgcaac ccctggagcc gcggccacac cccgggcgga tcctcaggtg gaagcgccgc ggcggtggcg gccgggctcg tcgccggcgc catcggttcg gacggggccg gaagcgtccg gatcccggcg gcctggacgc acctgatcgg tatcaagccg caacgcggcc gcatctccac ctggccgttt gccgaggcct tcaacgggat aacggtgcac ggaccgttgg cccgcacggt cgccgatgcc gcactgctgc tggacgcggc atcgggaaac gtccccgggg ncttgcacaa gccgccgcac gtccgggtgc tcgacgccgt ccacgangac ccgggacaac tgcgcgtcgc gttatcgctc aaggttccgt tctcgggttt cccggcgcaa ctgcatccga ccatcgaggc cgccacccgc tatgtggccc accagctgcg ttcactgggg cacatggngt ccnagggcga ccngnactat ggngtggggc tcggnccnnn nctttcngnc gcgagcgang nccggggcnn ctnncgnggn gcnatcnnnc tggnacnccn nancgcatng nnnncnnngn cnnnnngann annnnnnnnn nngnnnnnnc nnnnnnnggn ncnnnnnnnn nnnnnnnnnn nncnngnnnn cnngncnnnn nnnananngn nnnnnnannn nnnnnnnnnn c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGFPTLTS QAAALSDGSE SSVSLTLTAL NAIKSSQSTL NAFRVVCTKT ALAEATEADK RLARGERLPL LGVPIAIKDD TDLAGTPTAF GTAGAVGSES EDAELVRRLR AAGAVIVGKT NTCELGQWGF TSGPGFGHTR NPWSRGHTPG GSSGGSAAAV AAGLVAGAIG SDGAGSVRIP AAWTHLIGIK PQRGRISTWP FAEAFNGITV HGPLARTVAD AALLLDAASG NVPGDLHKPP HVRVLDAVHE DPGQLRVALS LKVPFSGFPA QLHPTIEAAT RYVAHQLRSL GHMVSEGDPD YGVGLGLNFL PRATAGLLPW RDRLDAGAHW DPRTVTNMRT GRRLAGWALR RARAAEPRLQ ARVGKIFGSF NVVLAPTTAQ PPTGIYDFDD VGSLATDRGQ VGACPMTWPW NVLGWPSINV PAGFTADGLP IGVQLMGPAN SEPLLISLAA QLESINNWAS EIPDPWW
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgggcttt cccacgctga cttcccaggc cgcagcgcta tctgacggtt cggagagttc ggtctcgctc accctcacgg cgctcaacgc gatcaagtcg agccagtcga ccctcaacgc attccgggtg gtgtgcacca agacggccct ggccgaagcc accgaggccg acaagcgatt agccaggggt gagcgcctgc cgttgctcgg agtgcccatc gccatcaagg acgacaccga cttggcgggc actcccaccg cattcggtac cgccggggcc gtcggctcgg agtccgagga cgccgagctg gtgcgccgat tgcgcgcggc gggcgcggtg atcgtcggca agaccaacac ctgcgagctg ggccagtggg gattcaccag cgggcccggc ttcgggcaca cccgcaaccc ctggagccgc ggccacaccc cgggcggatc ctcaggtgga agcgccgcgg cggtggcggc cgggctcgtc gccggcgcca tcggttcgga cggggccgga agcgtccgga tcccggcggc ctggacgcac ctgatcggta tcaagccgca acgcggccgc atctccacct ggccgtttgc cgaggccttc aacgggataa cggtgcacgg accgttggcc cgcacggtcg ccgatgccgc actgctgctg gacgcggcat cgggaaacgt ccccggggac ttgcacaagc cgccgcacgt ccgggtgctc gacgccgtcc acgaggaccc gggacaactg cgcgtcgcgt tatcgctcaa ggttccgttc tcgggtttcc cggcgcaact gcatccgacc atcgaggccg ccacccgcta tgtggcccac cagctgcgtt cactggggca catggtgtcc gagggcgacc cggactatgg cgtggggctc ggcctgaact ttctgccgcg agcgacggcc gggctgctgc cgtggcgcga tcggctggac gccggagcgc attgggaccc gcgcacggtg acgaatatgc gcaccggccg gcgcctagcc ggctgggcgc tgcggcgagc ccgcgccgcc gagccgcggc tgcaggcccg agtgggcaag atcttcggca gcttcaatgt ggtgttggca ccgacaaccg cccaaccgcc cacgggcatt tacgatttcg atgatgtcgg cagcctcgcc acggatcgcg ggcaggtcgg cgcgtgtccg atgacgtggc catggaacgt gctgggctgg ccgtcgatca atgtgcccgc cggcttcacg gccgacgggc tgccgatcgg tgtgcagttg atgggcccgg cgaacagcga gccgctgctg atttcccttg ccgcgcagct ggagtcgatc aacaattggg cgtcggagat ccccgatccc tggtggtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa