SSGCID: Target Page

MyabA.00951.c

Amidase family protein

CENTER ID:	MyabA.00951.c
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.00951.c.A1.GE34914	Full length( MyabA.00951.c )		1	455

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|36809.5.peg.4716
RefSeq:	YP_001705366.1
UniProt:	B1ML67

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEVADAVLRR IEKVNPLLNA FVFHNPEQVL RDARRLTEDL TKRTTLPPLY GIPYSVKEVC AVAATPVTSG VVAFKDMVAD RDEPVSARLR ASGGLFLGKT NIAEGGYKAS SDNHLYGSTR NPWHPGMTAG GSSSGAGAAV AAGMGQLAQG TDGGGSIRIP AALNGVVGFK PSLGRIPQTR LAGRFHTFAF HGPITRTVAD AALMLTTMAG FDDEDPLSLP SDGVDYLAAL DQPIESGRMA WSPDLGISKC DSEIQGICEE ALIAFVEMGW TVEEDRPDWT DPAKTMWEGV WGPVYAGKLD AANWDDQAGH VDQELVQVIR DGARLTTVQV QRADAARGKM VDTLREFLRR FDFLVTPVTT AQAFGHDEFC PAVLAGAPLL ERLMGWVLTY PFNMTTNPAI SIPVGFTRNG VPVGLQIVGR LRADADVLRL AAAFEAVRPW AQRRPSDAVL NGENP

NT Sequence

ATGGAGGTCG CCGACGCGGT ACTGCGGCGG ATCGAAAAGG TCAACCCGTT GCTCAACGCG TTTGTCTTTC ACAACCCGGA GCAGGTGCTG CGCGATGCCA GGAGGCTCAC CGAGGACCTC ACCAAACGCA CGACTCTGCC TCCACTGTAC GGAATTCCGT ACAGTGTCAA GGAGGTTTGC GCAGTTGCGG CAACACCGGT CACCTCAGGA GTCGTGGCCT TCAAGGATAT GGTCGCAGAC CGCGACGAGC CGGTGTCCGC TCGGTTGAGG GCATCCGGGG GCTTGTTCCT GGGAAAGACG AATATCGCGG AGGGCGGGTA CAAGGCCAGC TCCGACAATC ACCTCTACGG TTCCACGCGC AATCCTTGGC ATCCGGGAAT GACCGCGGGT GGCTCTAGTA GTGGTGCGGG AGCGGCGGTG GCGGCAGGTA TGGGCCAGCT CGCACAGGGA ACCGATGGCG GCGGCTCTAT CCGGATCCCG GCGGCCCTCA ATGGCGTGGT CGGATTCAAG CCTTCCCTCG GCCGTATTCC CCAAACCCGG CTTGCGGGCC GTTTTCACAC CTTTGCCTTC CACGGCCCGA TCACAAGGAC GGTGGCGGAT GCTGCGCTGA TGCTCACCAC GATGGCCGGC TTTGACGATG AGGACCCGTT ATCGTTGCCG TCGGATGGAG TCGATTACCT CGCTGCACTG GACCAGCCGA TCGAATCTGG GCGCATGGCG TGGTCACCGG ACCTTGGTAT ATCCAAGTGC GATAGCGAGA TTCAAGGAAT CTGTGAAGAA GCGCTCATCG CCTTTGTCGA GATGGGCTGG ACCGTTGAGG AGGATCGCCC GGACTGGACG GACCCTGCCA AGACCATGTG GGAAGGTGTG TGGGGGCCGG TCTATGCGGG CAAACTGGAT GCCGCGAATT GGGATGACCA GGCAGGCCAC GTCGATCAGG AACTTGTCCA GGTGATACGT GATGGCGCAC GGCTGACCAC CGTTCAGGTT CAGCGCGCGG ACGCGGCGAG GGGAAAGATG GTCGACACAT TGCGCGAATT CCTGCGCCGG TTCGACTTTC TCGTCACGCC CGTCACCACG GCACAAGCCT TCGGTCATGA CGAGTTCTGC CCCGCCGTGC TGGCCGGCGC TCCGCTACTG GAACGGCTGA TGGGGTGGGT CCTCACCTAT CCGTTCAATA TGACGACGAA TCCGGCGATT TCCATACCGG TCGGGTTCAC CAGGAATGGC GTGCCCGTCG GCTTGCAGAT TGTGGGCCGC CTCCGGGCAG ACGCCGACGT GCTGCGGCTA GCGGCAGCCT TCGAGGCCGT GCGGCCATGG GCTCAGCGTC GGCCGTCTGA CGCCGTGCTC AATGGCGAAA ACCCT

Details for MyabA.00951.c.A1.GE34914

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	57

Validated AA Sequence

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggagg tcgccgacgc ggtactgcgg cggatcgaaa aggtcaaccc gttgctcaac gcgtttgtct ttcacaaccc ggagcaggtg ctgcgcgatg ccaggaggct caccgaggac ctcaccaaac gcacgactct gcctccactg tacggaattc cgtacagtgt caaggaggtt tgcgcagttg cggcaacacc ggtcacctca ggagtcgtgg ccttcaagga tatggtcgca gaccgcgacg agccggtgtc cgctcggttg agggcatccg ggggcttgtt cctgggaaag acgaatatcg cggagggcgg gtacaaggcc agctccgaca atcacctcta cggttccacg cgcaatcctt ggcatccggg aatgaccgcg ggtggctcta gtagtggtgc gggagcggcg gtggcggcag gtatgggcca gctcgcacag ggaaccgatg gcggcggctc tatccggatc ccggcggccc tcaatggcgt ggtcggattc aagccttccc tcggccgtat tccccaaacc cggcttgcgg gccgttttca cacctttgcc ttccacggcc cgatcacaag gacggtggcg gatgctgcgc tgatgctcac cacgatggcc ggctttgacg atgaggancc gttatcgttg ccgtcggatg gagtcgatta cctcgctgca ctggaccagc cgatcgaatc tgggcgcatg gcgtggncac cggaccttgg tatatccaag tgcnatagcg aga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMEVADAVLR RIEKVNPLLN AFVFHNPEQV LRDARRLTED LTKRTTLPPL YGIPYSVKEV CAVAATPVTS GVVAFKDMVA DRDEPVSARL RASGGLFLGK TNIAEGGYKA SSDNHLYGST RNPWHPGMTA GGSSSGAGAA VAAGMGQLAQ GTDGGGSIRI PAALNGVVGF KPSLGRIPQT RLAGRFHTFA FHGPITRTVA DAALMLTTMA GFDDEDPLSL PSDGVDYLAA LDQPIESGRM AWSPDLGISK CDSEIQGICE EALIAFVEMG WTVEEDRPDW TDPAKTMWEG VWGPVYAGKL DAANWDDQAG HVDQELVQVI RDGARLTTVQ VQRADAARGK MVDTLREFLR RFDFLVTPVT TAQAFGHDEF CPAVLAGAPL LERLMGWVLT YPFNMTTNPA ISIPVGFTRN GVPVGLQIVG RLRADADVLR LAAAFEAVRP WAQRRPSDAV LNGENP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaggtcgcc gacgcggtac tgcggcggat cgaaaaggtc aacccgttgc tcaacgcgtt tgtctttcac aacccggagc aggtgctgcg cgatgccagg aggctcaccg aggacctcac caaacgcacg actctgcctc cactgtacgg aattccgtac agtgtcaagg aggtttgcgc agttgcggca acaccggtca cctcaggagt cgtggccttc aaggatatgg tcgcagaccg cgacgagccg gtgtccgctc ggttgagggc atccgggggc ttgttcctgg gaaagacgaa tatcgcggag ggcgggtaca aggccagctc cgacaatcac ctctacggtt ccacgcgcaa tccttggcat ccgggaatga ccgcgggtgg ctctagtagt ggtgcgggag cggcggtggc ggcaggtatg ggccagctcg cacagggaac cgatggcggc ggctctatcc ggatcccggc ggccctcaat ggcgtggtcg gattcaagcc ttccctcggc cgtattcccc aaacccggct tgcgggccgt tttcacacct ttgccttcca cggcccgatc acaaggacgg tggcggatgc tgcgctgatg ctcaccacga tggccggctt tgacgatgag gacccgttat cgttgccgtc ggatggagtc gattacctcg ctgcactgga ccagccgatc gaatctgggc gcatggcgtg gtcaccggac cttggtatat ccaagtgcga tagcgagatt caaggaatct gtgaagaagc gctcatcgcc tttgtcgaga tgggctggac cgttgaggag gatcgcccgg actggacgga ccctgccaag accatgtggg aaggtgtgtg ggggccggtc tatgcgggca aactggatgc cgcgaattgg gatgaccagg caggccacgt cgatcaggaa cttgtccagg tgatacgtga tggcgcacgg ctgaccaccg ttcaggttca gcgcgcggac gcggcgaggg gaaagatggt cgacacattg cgcgaattcc tgcgccggtt cgactttctc gtcacgcccg tcaccacggc acaagccttc ggtcatgacg agttctgccc cgccgtgctg gccggcgctc cgctactgga acggctgatg gggtgggtcc tcacctatcc gttcaatatg acgacgaatc cggcgatttc cataccggtc gggttcacca ggaatggcgt gcccgtcggc ttgcagattg tgggccgcct ccgggcagac gccgacgtgc tgcggctagc ggcagccttc gaggccgtgc ggccatgggc tcagcgtcgg ccgtctgacg ccgtgctcaa tggcgaaaac ccttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa